Rh5CG476900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0004466
Physical Location & Seq
Reverse (-)
66587160 .. 66593410
6251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG476900.1

Sequence Viewer

Length: 1143 bp
ATGGGAGGATTTGGCCCTGTTTATAAGATAAGAGACTTGGATAGGAAGAATGCAGAACTGGAAGAACAAAACAAAAATTTGGCTTCCATGCTTGAAACAAAGGAAGCCGGGAGTAATGTGCTGCAGAAACGTTTGAATGATCTGGAGCAAAATACTGTACCGTCTTTGAGGAAAGCTCTCAAGGATGTCGCAATGGAGAAAGATGCAGCAGTTGTTGCACGGGAGGATCTCTCTGGCCAGCTGCGTATGCTCAAGAAACGTCTAAAGGATGCAGAAGAAGAACAATATCGAGCTGAGGAAGATGCAGCATCCCTGAGAGCAGAACTCAATATGATACAGCAACAAACAATGAATGGCTCACTTGGTGCACTAAACTCAATTGCTAATCCACCTGATCAGATAAAAACATTAGAAATTGAGTTAGCTAACATAAAGTCCGAGTTACAGCGCGAGTCAATGTTTAGACAGCAAGAACGACAACAATTAGGAGAGGAGCAGGCTCGAGCATCCATCCTTACATCTGAGAAGCAAGAATTGGAAGAAAAACTTGCAGCTTTGTCTAGAAAGTCCTCAGAAGTGTCAGACAAAGTGGCCAGCATGGGATTTTCATTAGAAGACAAGGAGGTACTCGAGAAGCAGATGCATGACATGGCTGTAGTGATTGAAAAATTGGAAAGCAGTAGACAAAAGCTTTTGATGGAGATTGATTCCCAATCTTCGGAGATAGAAAGGCTTTTTGAGGAAAACTCTAATTTATCTAGTTCTCATCAGGAGGCTATGAGCATGGCTTTGCACTGGGAGAATCAGGTGAAAGACTGTCTTAGGCAAAATGAAGAGCTCCGAGGAATCTTAGATAAGTTAAGAACTGACCAGGCCAAGGGGTTGCTCGACTCCCATAGCAATGGTTCTAACAAGACTGGTTTAGCTGAATACACACCTGAAGTTCTCTCTATTAAGGATCAACTTGCAAAAGAACAGAGCAAAGCAGAGGCATTATCAGCTGAGGTATTACAGCTCTCAGCAAGACTCCAACAAACCACTCAAGCATACAATGGTCTTGCACGCCTCTATAAACCGGTGTTGCGAAACATAGAAAGCAGTCTCATTAAGATGAAACAAGATGGCCCTGTGACAGTGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

380

Amino Acids

42.86

Weight (kDa)

5.24

Isoelectric Point (pI)

60.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 24
AccI GTMKAC 1 cut(s) 682
AccII CGCG 1 cut(s) 450
AciI CCGC 1 cut(s) 1138
AclI AACGTT 1 cut(s) 130
AclWI GGATC 2 cut(s) 234, 966
AcoI YGGCCR 2 cut(s) 235, 591
AcsI RAATTY 1 cut(s) 76
AcuI CTGAAG 1 cut(s) 960
AdeI CACNNNGTG 1 cut(s) 365
AfaI GTAC 2 cut(s) 159, 627
AfiI CCNNNNNNNGG 2 cut(s) 718, 877
AgeI ACCGGT 1 cut(s) 1075
AgsI TTSAA 3 cut(s) 95, 136, 665
AjnI CCWGG 1 cut(s) 870
AjuI GAANNNNNNNTTGG 2 cut(s) 518, 550
Alw21I GWGCWC 2 cut(s) 370, 840
Alw26I GTCTC 2 cut(s) 27, 1106
Alw44I GTGCAC 1 cut(s) 366
AlwI GGATC 2 cut(s) 234, 966
Ama87I CYCGRG 2 cut(s) 501, 629
AoxI GGCC 5 cut(s) 13, 235, 591, 873, 1123
ApaLI GTGCAC 1 cut(s) 366
ApeKI GCWGC 5 cut(s) 121, 206, 241, 305, 551
ApoI RAATTY 1 cut(s) 76
AsiGI ACCGGT 1 cut(s) 1075
AspLEI GCGC 1 cut(s) 450
AspS9I GGNCC 2 cut(s) 14, 1124
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 820
AvaI CYCGRG 2 cut(s) 501, 629
BaeGI GKGCMC 1 cut(s) 370
BalI TGGCCA 2 cut(s) 237, 593
BanII GRGCYC 1 cut(s) 840
BbsI GAAGAC 1 cut(s) 621
Bbv12I GWGCWC 2 cut(s) 370, 840
BbvCI CCTCAGC 2 cut(s) 294, 1002
BbvI GCAGC 5 cut(s) 108, 218, 228, 317, 563
BccI CCATC 3 cut(s) 518, 691, 1115
BciT130I CCWGG 1 cut(s) 872
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 27, 1106
BfaI CTAG 2 cut(s) 561, 759
BfmI CTRYAG 2 cut(s) 122, 654
BisI GCNGC 5 cut(s) 122, 207, 242, 306, 552
BlsI GCNGC 5 cut(s) 123, 208, 243, 307, 553
Bme1390I CCNGG 2 cut(s) 109, 872
BmeT110I CYCGRG 2 cut(s) 501, 629
BmgT120I GGNCC 2 cut(s) 14, 1124
BmrFI CCNGG 2 cut(s) 109, 872
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 6 cut(s) 193, 259, 292, 317, 515, 630
BmuI ACTGGG 1 cut(s) 805
BpiI GAAGAC 1 cut(s) 621
BplI GAGNNNNNCTC 8 cut(s) 160, 192, 215, 247, 309, 341, 731, 763
BpmI CTGGAG 1 cut(s) 164
Bpu10I CCTNAGC 2 cut(s) 294, 1002
BpuEI CTTGAG 3 cut(s) 164, 236, 1026
BpuMI CCSGG 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 841, 876
BsaWI WCCGGW 1 cut(s) 1075
Bsc4I CCNNNNNNNGG 2 cut(s) 718, 877
Bse118I RCCGGY 1 cut(s) 1075
Bse1I ACTGG 3 cut(s) 63, 800, 922
Bse3DI GCAATG 2 cut(s) 198, 907
BseBI CCWGG 1 cut(s) 872
BseDI CCNNGG 2 cut(s) 841, 876
BseGI GGATG 5 cut(s) 190, 274, 308, 506, 510
BseLI CCNNNNNNNGG 2 cut(s) 718, 877
BseMI GCAATG 2 cut(s) 198, 907
BseMII CTCAG 6 cut(s) 285, 305, 513, 585, 993, 1032
BseNI ACTGG 3 cut(s) 63, 800, 922
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 1 cut(s) 370
BseXI GCAGC 5 cut(s) 108, 218, 228, 317, 563
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 5 cut(s) 15, 237, 593, 875, 1125
BshTI ACCGGT 1 cut(s) 1075
BsiHKAI GWGCWC 2 cut(s) 370, 840
BsiHKCI CYCGRG 2 cut(s) 501, 629
BsiSI CCGG 2 cut(s) 108, 1076
BslI CCNNNNNNNGG 2 cut(s) 718, 877
BsmAI GTCTC 2 cut(s) 27, 1106
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 5 cut(s) 15, 237, 593, 875, 1125
BsoBI CYCGRG 2 cut(s) 501, 629
Bsp1286I GDGCHC 2 cut(s) 370, 840
Bsp143I GATC 4 cut(s) 139, 226, 394, 958
BspACI CCGC 1 cut(s) 1138
BspANI GGCC 5 cut(s) 15, 237, 593, 875, 1125
BspCNI CTCAG 6 cut(s) 286, 306, 514, 584, 994, 1031
BspFNI CGCG 1 cut(s) 450
BspMAI CTGCAG 1 cut(s) 126
BspPI GGATC 2 cut(s) 234, 966
BspQI GCTCTTC 1 cut(s) 828
BsrDI GCAATG 2 cut(s) 198, 907
BsrFI RCCGGY 1 cut(s) 1075
BsrI ACTGG 3 cut(s) 63, 800, 922
BssAI RCCGGY 1 cut(s) 1075
BssECI CCNNGG 2 cut(s) 841, 876
BssMI GATC 4 cut(s) 139, 226, 394, 958
BssT1I CCWWGG 1 cut(s) 876
Bst2UI CCWGG 1 cut(s) 872
Bst4CI ACNGT 4 cut(s) 157, 162, 818, 1135
Bst6I CTCTTC 1 cut(s) 828
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 4 cut(s) 239, 498, 595, 1063
BstDEI CTNAG 8 cut(s) 294, 314, 522, 571, 821, 850, 1002, 1018
BstF5I GGATG 5 cut(s) 190, 274, 308, 506, 510
BstFNI CGCG 1 cut(s) 450
BstHHI GCGC 1 cut(s) 450
BstKTI GATC 4 cut(s) 142, 229, 397, 961
BstMAI GTCTC 2 cut(s) 27, 1106
BstMBI GATC 4 cut(s) 139, 226, 394, 958
BstMWI GCNNNNNNNGC 3 cut(s) 215, 247, 998
BstNI CCWGG 1 cut(s) 872
BstSCI CCNGG 2 cut(s) 107, 870
BstSFI CTRYAG 2 cut(s) 122, 654
BstSLI GKGCMC 1 cut(s) 370
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 5 cut(s) 108, 218, 228, 317, 563
BstV2I GAAGAC 1 cut(s) 621
BstX2I RGATCY 1 cut(s) 226
BstXI CCANNNNNNTGG 1 cut(s) 902
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 5 cut(s) 15, 237, 593, 875, 1125
BtsCI GGATG 5 cut(s) 190, 274, 308, 506, 510
BtsIMutI CAGTG 2 cut(s) 793, 1140
Cac8I GCNNGC 4 cut(s) 239, 498, 595, 1063
CfoI GCGC 1 cut(s) 450
Cfr10I RCCGGY 1 cut(s) 1075
Cfr13I GGNCC 2 cut(s) 14, 1124
Csp6I GTAC 2 cut(s) 158, 626
CspAI ACCGGT 1 cut(s) 1075
CviAII CATG 5 cut(s) 88, 598, 644, 649, 784
CviQI GTAC 2 cut(s) 158, 626
DdeI CTNAG 8 cut(s) 294, 314, 522, 571, 821, 850, 1002, 1018
DpnI GATC 4 cut(s) 141, 228, 396, 960
DpnII GATC 4 cut(s) 139, 226, 394, 958
DraIII CACNNNGTG 1 cut(s) 365
EaeI YGGCCR 2 cut(s) 235, 591
Eam1104I CTCTTC 1 cut(s) 828
EarI CTCTTC 1 cut(s) 828
Ecl136II GAGCTC 1 cut(s) 838
Eco130I CCWWGG 1 cut(s) 876
Eco24I GRGCYC 1 cut(s) 840
Eco53kI GAGCTC 1 cut(s) 838
Eco57I CTGAAG 1 cut(s) 960
Eco88I CYCGRG 2 cut(s) 501, 629
EcoICRI GAGCTC 1 cut(s) 838
EcoRII CCWGG 1 cut(s) 870
EcoT14I CCWWGG 1 cut(s) 876
EcoT22I ATGCAT 1 cut(s) 645
EcoT38I GRGCYC 1 cut(s) 840
ErhI CCWWGG 1 cut(s) 876
FaeI CATG 5 cut(s) 91, 601, 647, 652, 787
FalI AAGNNNNNCTT 4 cut(s) 531, 563, 804, 836
FatI CATG 5 cut(s) 87, 597, 643, 648, 783
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 5 cut(s) 122, 207, 242, 306, 552
FokI GGATG 5 cut(s) 197, 281, 295, 493, 497
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 5 cut(s) 122, 207, 242, 306, 552
FspBI CTAG 2 cut(s) 561, 759
GlaI GCGC 1 cut(s) 449
GluI GCNGC 5 cut(s) 122, 207, 242, 306, 552
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 5 cut(s) 15, 237, 593, 875, 1125
HapII CCGG 2 cut(s) 108, 1076
HhaI GCGC 1 cut(s) 450
Hin1II CATG 5 cut(s) 91, 601, 647, 652, 787
Hin6I GCGC 1 cut(s) 448
HinP1I GCGC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 6 cut(s) 452, 707, 802, 846, 890, 1026
HpaII CCGG 2 cut(s) 108, 1076
HphI GGTGA 1 cut(s) 820
Hpy166II GTNNAC 2 cut(s) 368, 683
Hpy188I TCNGA 7 cut(s) 399, 439, 523, 574, 583, 721, 842
Hpy188III TCNNGA 5 cut(s) 143, 253, 561, 631, 770
Hpy8I GTNNAC 2 cut(s) 368, 683
HpyCH4III ACNGT 4 cut(s) 157, 162, 818, 1135
HpyCH4IV ACGT 2 cut(s) 130, 259
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 247, 998
HpyF3I CTNAG 8 cut(s) 294, 314, 522, 571, 821, 850, 1002, 1018
HpySE526I ACGT 2 cut(s) 130, 259
Hsp92II CATG 5 cut(s) 91, 601, 647, 652, 787
HspAI GCGC 1 cut(s) 448
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 4 cut(s) 139, 226, 394, 958
LguI GCTCTTC 1 cut(s) 828
LmnI GCTCC 3 cut(s) 145, 493, 843
Lsp1109I GCAGC 5 cut(s) 108, 218, 228, 317, 563
LweI GCATC 6 cut(s) 193, 259, 292, 317, 515, 630
MaeI CTAG 2 cut(s) 561, 759
MaeII ACGT 2 cut(s) 130, 259
MaeIII GTNAC 2 cut(s) 441, 1129
MalI GATC 4 cut(s) 141, 228, 396, 960
MboI GATC 4 cut(s) 139, 226, 394, 958
MboII GAAGA 9 cut(s) 58, 74, 287, 290, 311, 551, 626, 708, 845
MfeI CAATTG 1 cut(s) 378
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 2 cut(s) 370, 840
MlsI TGGCCA 2 cut(s) 237, 593
MluCI AATT 7 cut(s) 76, 378, 414, 482, 533, 668, 751
MluNI TGGCCA 2 cut(s) 237, 593
MlyI GAGTC 3 cut(s) 461, 884, 1020
MmeI TCCRAC 1 cut(s) 1054
Mox20I TGGCCA 2 cut(s) 237, 593
Mph1103I ATGCAT 1 cut(s) 645
MscI TGGCCA 2 cut(s) 237, 593
MseI TTAA 3 cut(s) 860, 954, 1107
MslI CAYNNNNRTG 2 cut(s) 900, 1109
Msp20I TGGCCA 2 cut(s) 237, 593
MspA1I CMGCKG 2 cut(s) 241, 1001
MspI CCGG 2 cut(s) 108, 1076
MspR9I CCNGG 2 cut(s) 109, 872
MunI CAATTG 1 cut(s) 378
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 1 cut(s) 872
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 3 cut(s) 215, 247, 998
NciI CCSGG 1 cut(s) 109
NdeII GATC 4 cut(s) 139, 226, 394, 958
NlaIII CATG 5 cut(s) 91, 601, 647, 652, 787
NmuCI GTSAC 1 cut(s) 1129
NsiI ATGCAT 1 cut(s) 645
PaeR7I CTCGAG 2 cut(s) 501, 629
PciSI GCTCTTC 1 cut(s) 828
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 707, 802, 846
PinAI ACCGGT 1 cut(s) 1075
PkrI GCNGC 5 cut(s) 123, 208, 243, 307, 553
PleI GAGTC 3 cut(s) 460, 884, 1020
PpsI GAGTC 3 cut(s) 460, 884, 1020
PsiI TTATAA 1 cut(s) 24
Psp124BI GAGCTC 1 cut(s) 840
Psp1406I AACGTT 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 870
PspGI CCWGG 1 cut(s) 870
PspPI GGNCC 2 cut(s) 14, 1124
PspXI VCTCGAGB 1 cut(s) 501
PstI CTGCAG 1 cut(s) 126
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 2 cut(s) 241, 1001
RsaI GTAC 2 cut(s) 159, 627
RsaNI GTAC 2 cut(s) 158, 626
RseI CAYNNNNRTG 2 cut(s) 900, 1109
SacI GAGCTC 1 cut(s) 840
SapI GCTCTTC 1 cut(s) 828
SaqAI TTAA 3 cut(s) 860, 954, 1107
SatI GCNGC 5 cut(s) 122, 207, 242, 306, 552
Sau3AI GATC 4 cut(s) 139, 226, 394, 958
Sau96I GGNCC 2 cut(s) 14, 1124
SchI GAGTC 3 cut(s) 461, 884, 1020
ScrFI CCNGG 2 cut(s) 109, 872
SduI GDGCHC 2 cut(s) 370, 840
SfaNI GCATC 6 cut(s) 193, 259, 292, 317, 515, 630
SfcI CTRYAG 2 cut(s) 122, 654
Sfr274I CTCGAG 2 cut(s) 501, 629
SlaI CTCGAG 2 cut(s) 501, 629
SmiMI CAYNNNNRTG 2 cut(s) 900, 1109
SmlI CTYRAG 5 cut(s) 179, 251, 501, 629, 1041
SmoI CTYRAG 5 cut(s) 179, 251, 501, 629, 1041
Sse9I AATT 7 cut(s) 76, 378, 414, 482, 533, 668, 751
SsiI CCGC 1 cut(s) 1138
SspMI CTAG 2 cut(s) 561, 759
SstI GAGCTC 1 cut(s) 840
StyD4I CCNGG 2 cut(s) 107, 870
StyI CCWWGG 1 cut(s) 876
TaaI ACNGT 4 cut(s) 157, 162, 818, 1135
TaiI ACGT 2 cut(s) 133, 262
TaqI TCGA 4 cut(s) 289, 502, 630, 888
TasI AATT 7 cut(s) 76, 378, 414, 482, 533, 668, 751
TfiI GAWTC 3 cut(s) 707, 802, 846
Tru1I TTAA 3 cut(s) 860, 954, 1107
Tru9I TTAA 3 cut(s) 860, 954, 1107
TscAI CASTG 2 cut(s) 800, 1140
TseFI GTSAC 1 cut(s) 1129
TseI GCWGC 5 cut(s) 121, 206, 241, 305, 551
Tsp45I GTSAC 1 cut(s) 1129
TspDTI ATGAA 4 cut(s) 365, 597, 846, 1127
TspRI CASTG 2 cut(s) 800, 1140
VneI GTGCAC 1 cut(s) 366
XapI RAATTY 1 cut(s) 76
XbaI TCTAGA 1 cut(s) 560
XhoI CTCGAG 2 cut(s) 501, 629
XmiI GTMKAC 1 cut(s) 682
XspI CTAG 2 cut(s) 561, 759
Zsp2I ATGCAT 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.