Rroxscaffold_1G00014280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004466
Physical Location & Seq
Forward (+)
17593203 .. 17597530
4328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00014280.1

Sequence Viewer

Length: 1326 bp
ATGGATTCCCGGCACGCATCTCTAGGCCGCCGAACGTTGGAAGAAATTCGTCAAAAGCGAGCTGCAGAAAGATTGAGCAAAACTTCTTCGGGGCCCGATCTCAGCCAGATCCCACCTATCGGTAATGGAGGAATGAGAAAATCGGAGAGCGCAAATCGACTCTCTGAGAGCGATGTTAGTACTTTAGTTTCTCAGATAAGAGAGTTGGATAGGAAGAATGCAGAACTGGAAGAACAGAACAAAAATTTGGCTTCCATGCTTGAAACAAAGGAAGCCGGGACTACTGTGCTGCAGAAACGTTTGAATGATCTGGAGCAAAATACTGTACCGTCTTTGAGGAAAGCTCTCAAGGATGTCGCAATGGAGAAAGATGCAGCAGTTGTTGCACGGGAGGATCTCTCTGCCCAGCTGCGTATGCTCAAGAAACGTCTAAAGGACGCAGAAGAAGAACAGTATCGAGCTGAGGAAGATGCAGCATCCCTGAGAGCAGAACTCAATATGATACAGCAACAAACAATGAATGGCTCACTTGGTGCACTAAACTCAATTGCTAATCCACCTGATCAGATAAAAACATTAGAAATTGAGTTAGCTAACATAAAGTCTGAGTTACAGCGCGAGTCAATGTTTAGACAGCAAGAACGACAACAATTAGGAGAGGAGCAGGCTCGAGCATCCATCCTTACATCTGAGAAGCAAGAATTGGAAGAAAAACTTGCAGCTTTGTCTAGAAAGTCCTCAGAAGTGTCAGACAAAGTGGCCAGCAAGGGATTTTCATTATGCATTCATGGCCAGGAAGACAAGGAGATACTCGAGAAGCAGATGCATGACATGGCTGTAGTGATTGAGAAATTGGAAAACAGTCGACAAAAGCTGTTAATGGAGATTGATTCCCAATCCTCAGAGATAGAAAGGCTTTTTGAGGAAAACTCTAATTTATCTAGTTCTCATCAGGAGGCAATGAGCATAGCTGTGCACTGGGAGAATCAGGTGAAAGACTGTCTTAAGCAAAATGAAGAGCTCCGAGGAATCTTAGATAAGTTAAGAACTGACCAGGCCAAGGGGTTGCTTGACTCCCATAACAATGGCTCTAACAAGACCGGTTTAGCTGAATATACACCTGAAGTTCTCTCTATCAAGGAACAACTTGCAAAAGAACAGAGCAAAGCAGAGGCATTATCAGCTGAGGTATTACAGCTCTCAGCAAGACTCCAACAAACCACTCAAGCATACAATGGGCTTGCACGCCTCTATAAACCGGTGTTGCGAAACATAGAAAGCAGTCTCATTAAGATGAAACAAGATGGCCCTGTGACAGTGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

441

Amino Acids

49.57

Weight (kDa)

5.62

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 865
AccII CGCG 1 cut(s) 618
AciI CCGC 2 cut(s) 28, 1321
AclI AACGTT 2 cut(s) 35, 298
AclWI GGATC 2 cut(s) 103, 402
AcoI YGGCCR 2 cut(s) 759, 790
AcsI RAATTY 2 cut(s) 45, 244
AcuI CTGAAG 1 cut(s) 1143
AdeI CACNNNGTG 1 cut(s) 533
AfaI GTAC 2 cut(s) 181, 327
AfiI CCNNNNNNNGG 3 cut(s) 37, 119, 1060
AflII CTTAAG 1 cut(s) 1004
AgeI ACCGGT 2 cut(s) 1100, 1258
AgsI TTSAA 2 cut(s) 263, 304
AjnI CCWGG 2 cut(s) 792, 1053
AjuI GAANNNNNNNTTGG 4 cut(s) 230, 262, 686, 718
Alw21I GWGCWC 3 cut(s) 538, 978, 1023
Alw26I GTCTC 1 cut(s) 1289
Alw44I GTGCAC 2 cut(s) 534, 974
AlwI GGATC 2 cut(s) 103, 402
Ama87I CYCGRG 2 cut(s) 669, 812
AoxI GGCC 6 cut(s) 25, 92, 759, 790, 1056, 1306
ApaI GGGCCC 1 cut(s) 96
ApaLI GTGCAC 2 cut(s) 534, 974
ApeKI GCWGC 6 cut(s) 62, 289, 374, 409, 473, 719
ApoI RAATTY 2 cut(s) 45, 244
AsiGI ACCGGT 2 cut(s) 1100, 1258
Asp700I GAANNNNTTC 1 cut(s) 45
AspLEI GCGC 2 cut(s) 152, 618
AspS9I GGNCC 3 cut(s) 92, 93, 1307
AsuC2I CCSGG 2 cut(s) 10, 277
AsuHPI GGTGA 1 cut(s) 1003
AvaI CYCGRG 2 cut(s) 669, 812
BaeGI GKGCMC 3 cut(s) 96, 538, 978
BalI TGGCCA 2 cut(s) 761, 792
BanII GRGCYC 2 cut(s) 96, 1023
BbsI GAAGAC 1 cut(s) 804
Bbv12I GWGCWC 3 cut(s) 538, 978, 1023
BbvCI CCTCAGC 2 cut(s) 462, 1185
BbvI GCAGC 6 cut(s) 49, 276, 386, 396, 485, 731
BccI CCATC 2 cut(s) 686, 1298
BciT130I CCWGG 2 cut(s) 794, 1055
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 2 cut(s) 10, 277
BcoDI GTCTC 1 cut(s) 1289
BfaI CTAG 3 cut(s) 23, 729, 942
BfmI CTRYAG 3 cut(s) 63, 290, 837
BfrI CTTAAG 1 cut(s) 1004
BisI GCNGC 7 cut(s) 28, 63, 290, 375, 410, 474, 720
BlsI GCNGC 7 cut(s) 29, 64, 291, 376, 411, 475, 721
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 4 cut(s) 10, 277, 794, 1055
BmeT110I CYCGRG 2 cut(s) 669, 812
BmgT120I GGNCC 3 cut(s) 92, 93, 1307
BmiI GGNNCC 2 cut(s) 93, 94
BmrFI CCNGG 4 cut(s) 10, 277, 794, 1055
BmrI ACTGGG 1 cut(s) 988
BmsI GCATC 6 cut(s) 26, 361, 460, 485, 683, 813
BmuI ACTGGG 1 cut(s) 988
BpiI GAAGAC 1 cut(s) 804
BplI GAGNNNNNCTC 8 cut(s) 328, 360, 383, 415, 477, 509, 914, 946
BpmI CTGGAG 1 cut(s) 332
Bpu10I CCTNAGC 2 cut(s) 462, 1185
BpuEI CTTGAG 3 cut(s) 332, 404, 1209
BpuMI CCSGG 2 cut(s) 10, 277
BsaJI CCNNGG 2 cut(s) 1024, 1059
BsaWI WCCGGW 2 cut(s) 1100, 1258
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 119, 1060
Bse118I RCCGGY 2 cut(s) 1100, 1258
Bse1I ACTGG 2 cut(s) 231, 983
Bse3DI GCAATG 2 cut(s) 366, 966
BseBI CCWGG 2 cut(s) 794, 1055
BseDI CCNNGG 2 cut(s) 1024, 1059
BseGI GGATG 4 cut(s) 358, 476, 674, 678
BseLI CCNNNNNNNGG 3 cut(s) 37, 119, 1060
BseMI GCAATG 2 cut(s) 366, 966
BseNI ACTGG 2 cut(s) 231, 983
BseRI GAGGAG 1 cut(s) 674
BseSI GKGCMC 3 cut(s) 96, 538, 978
BseXI GCAGC 6 cut(s) 49, 276, 386, 396, 485, 731
BseYI CCCAGC 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 618
BshFI GGCC 6 cut(s) 27, 94, 761, 792, 1058, 1308
BshTI ACCGGT 2 cut(s) 1100, 1258
BsiHKAI GWGCWC 3 cut(s) 538, 978, 1023
BsiHKCI CYCGRG 2 cut(s) 669, 812
BsiSI CCGG 4 cut(s) 10, 276, 1101, 1259
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 3 cut(s) 37, 119, 1060
BsmAI GTCTC 1 cut(s) 1289
BsmFI GGGAC 1 cut(s) 292
BsmI GAATGC 2 cut(s) 223, 783
BsnI GGCC 6 cut(s) 27, 94, 761, 792, 1058, 1308
BsoBI CYCGRG 2 cut(s) 669, 812
Bsp120I GGGCCC 1 cut(s) 92
Bsp1286I GDGCHC 4 cut(s) 96, 538, 978, 1023
Bsp143I GATC 5 cut(s) 97, 108, 307, 394, 562
BspACI CCGC 2 cut(s) 28, 1321
BspANI GGCC 6 cut(s) 27, 94, 761, 792, 1058, 1308
BspFNI CGCG 1 cut(s) 618
BspLI GGNNCC 2 cut(s) 93, 94
BspMAI CTGCAG 2 cut(s) 67, 294
BspPI GGATC 2 cut(s) 103, 402
BspQI GCTCTTC 1 cut(s) 1011
BspTI CTTAAG 1 cut(s) 1004
BsrDI GCAATG 2 cut(s) 366, 966
BsrFI RCCGGY 2 cut(s) 1100, 1258
BsrI ACTGG 2 cut(s) 231, 983
BssAI RCCGGY 2 cut(s) 1100, 1258
BssECI CCNNGG 2 cut(s) 1024, 1059
BssMI GATC 5 cut(s) 97, 108, 307, 394, 562
BssT1I CCWWGG 1 cut(s) 1059
Bst2UI CCWGG 2 cut(s) 794, 1055
Bst4CI ACNGT 7 cut(s) 286, 325, 330, 453, 863, 1001, 1318
Bst6I CTCTTC 1 cut(s) 1011
BstAFI CTTAAG 1 cut(s) 1004
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 6 cut(s) 15, 60, 666, 763, 1242, 1246
BstF5I GGATG 4 cut(s) 358, 476, 674, 678
BstFNI CGCG 1 cut(s) 618
BstHHI GCGC 2 cut(s) 152, 618
BstKTI GATC 5 cut(s) 100, 111, 310, 397, 565
BstMAI GTCTC 1 cut(s) 1289
BstMBI GATC 5 cut(s) 97, 108, 307, 394, 562
BstMWI GCNNNNNNNGC 4 cut(s) 383, 415, 789, 1181
BstNI CCWGG 2 cut(s) 794, 1055
BstSCI CCNGG 4 cut(s) 8, 275, 792, 1053
BstSFI CTRYAG 3 cut(s) 63, 290, 837
BstSLI GKGCMC 3 cut(s) 96, 538, 978
BstUI CGCG 1 cut(s) 618
BstV1I GCAGC 6 cut(s) 49, 276, 386, 396, 485, 731
BstV2I GAAGAC 1 cut(s) 804
BstX2I RGATCY 2 cut(s) 108, 394
BstXI CCANNNNNNTGG 1 cut(s) 1085
BstYI RGATCY 2 cut(s) 108, 394
BsuRI GGCC 6 cut(s) 27, 94, 761, 792, 1058, 1308
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 4 cut(s) 358, 476, 674, 678
BtsIMutI CAGTG 2 cut(s) 976, 1323
Cac8I GCNNGC 6 cut(s) 15, 60, 666, 763, 1242, 1246
CfoI GCGC 2 cut(s) 152, 618
Cfr10I RCCGGY 2 cut(s) 1100, 1258
Cfr13I GGNCC 3 cut(s) 92, 93, 1307
CseI GACGC 1 cut(s) 446
Csp6I GTAC 2 cut(s) 180, 326
CspAI ACCGGT 2 cut(s) 1100, 1258
CviAII CATG 4 cut(s) 256, 788, 827, 832
CviQI GTAC 2 cut(s) 180, 326
DpnI GATC 5 cut(s) 99, 110, 309, 396, 564
DpnII GATC 5 cut(s) 97, 108, 307, 394, 562
DraIII CACNNNGTG 1 cut(s) 533
EaeI YGGCCR 2 cut(s) 759, 790
Eam1104I CTCTTC 1 cut(s) 1011
EarI CTCTTC 1 cut(s) 1011
Ecl136II GAGCTC 1 cut(s) 1021
Eco130I CCWWGG 1 cut(s) 1059
Eco24I GRGCYC 2 cut(s) 96, 1023
Eco53kI GAGCTC 1 cut(s) 1021
Eco57I CTGAAG 1 cut(s) 1143
Eco88I CYCGRG 2 cut(s) 669, 812
EcoICRI GAGCTC 1 cut(s) 1021
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 2 cut(s) 792, 1053
EcoT14I CCWWGG 1 cut(s) 1059
EcoT22I ATGCAT 2 cut(s) 785, 828
EcoT38I GRGCYC 2 cut(s) 96, 1023
ErhI CCWWGG 1 cut(s) 1059
FaeI CATG 4 cut(s) 259, 791, 830, 835
FalI AAGNNNNNCTT 4 cut(s) 699, 731, 987, 1019
FaqI GGGAC 1 cut(s) 292
FatI CATG 4 cut(s) 255, 787, 826, 831
FbaI TGATCA 1 cut(s) 562
FblI GTMKAC 1 cut(s) 865
Fnu4HI GCNGC 7 cut(s) 28, 63, 290, 375, 410, 474, 720
FokI GGATG 4 cut(s) 365, 463, 661, 665
FriOI GRGCYC 2 cut(s) 96, 1023
Fsp4HI GCNGC 7 cut(s) 28, 63, 290, 375, 410, 474, 720
FspBI CTAG 3 cut(s) 23, 729, 942
GlaI GCGC 2 cut(s) 151, 617
GluI GCNGC 7 cut(s) 28, 63, 290, 375, 410, 474, 720
GsaI CCCAGC 1 cut(s) 409
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 6 cut(s) 27, 94, 761, 792, 1058, 1308
HapII CCGG 4 cut(s) 10, 276, 1101, 1259
HgaI GACGC 1 cut(s) 446
HhaI GCGC 2 cut(s) 152, 618
Hin1II CATG 4 cut(s) 259, 791, 830, 835
Hin6I GCGC 2 cut(s) 150, 616
HinP1I GCGC 2 cut(s) 150, 616
HincII GTYRAC 1 cut(s) 866
HindII GTYRAC 1 cut(s) 866
HinfI GANTC 8 cut(s) 5, 159, 620, 890, 985, 1029, 1073, 1209
HpaII CCGG 4 cut(s) 10, 276, 1101, 1259
HphI GGTGA 1 cut(s) 1003
Hpy166II GTNNAC 3 cut(s) 536, 866, 976
Hpy188III TCNNGA 5 cut(s) 311, 421, 729, 814, 953
Hpy8I GTNNAC 3 cut(s) 536, 866, 976
HpyCH4III ACNGT 7 cut(s) 286, 325, 330, 453, 863, 1001, 1318
HpyCH4IV ACGT 3 cut(s) 35, 298, 427
HpyF10VI GCNNNNNNNGC 4 cut(s) 383, 415, 789, 1181
HpySE526I ACGT 3 cut(s) 35, 298, 427
Hsp92II CATG 4 cut(s) 259, 791, 830, 835
HspAI GCGC 2 cut(s) 150, 616
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 5 cut(s) 97, 108, 307, 394, 562
LguI GCTCTTC 1 cut(s) 1011
LmnI GCTCC 3 cut(s) 313, 661, 1026
Lsp1109I GCAGC 6 cut(s) 49, 276, 386, 396, 485, 731
LweI GCATC 6 cut(s) 26, 361, 460, 485, 683, 813
MaeI CTAG 3 cut(s) 23, 729, 942
MaeII ACGT 3 cut(s) 35, 298, 427
MaeIII GTNAC 2 cut(s) 609, 1312
MalI GATC 5 cut(s) 99, 110, 309, 396, 564
MboI GATC 5 cut(s) 97, 108, 307, 394, 562
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 2 cut(s) 108, 394
MhlI GDGCHC 4 cut(s) 96, 538, 978, 1023
MlsI TGGCCA 2 cut(s) 761, 792
MluCI AATT 8 cut(s) 45, 244, 546, 582, 650, 701, 851, 934
MluNI TGGCCA 2 cut(s) 761, 792
MlyI GAGTC 4 cut(s) 153, 629, 1067, 1203
MmeI TCCRAC 3 cut(s) 18, 186, 1237
Mox20I TGGCCA 2 cut(s) 761, 792
Mph1103I ATGCAT 2 cut(s) 785, 828
MroXI GAANNNNTTC 1 cut(s) 45
MscI TGGCCA 2 cut(s) 761, 792
MseI TTAA 4 cut(s) 878, 1005, 1043, 1290
MslI CAYNNNNRTG 3 cut(s) 971, 1083, 1292
Msp20I TGGCCA 2 cut(s) 761, 792
MspA1I CMGCKG 2 cut(s) 409, 1184
MspCI CTTAAG 1 cut(s) 1004
MspI CCGG 4 cut(s) 10, 276, 1101, 1259
MspR9I CCNGG 4 cut(s) 10, 277, 794, 1055
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 2 cut(s) 223, 783
MvaI CCWGG 2 cut(s) 794, 1055
MvnI CGCG 1 cut(s) 618
MwoI GCNNNNNNNGC 4 cut(s) 383, 415, 789, 1181
NciI CCSGG 2 cut(s) 10, 277
NdeII GATC 5 cut(s) 97, 108, 307, 394, 562
NlaIII CATG 4 cut(s) 259, 791, 830, 835
NlaIV GGNNCC 2 cut(s) 93, 94
NmuCI GTSAC 1 cut(s) 1312
NsiI ATGCAT 2 cut(s) 785, 828
PaeR7I CTCGAG 2 cut(s) 669, 812
PciSI GCTCTTC 1 cut(s) 1011
PcsI WCGNNNNNNNCGW 1 cut(s) 55
PctI GAATGC 2 cut(s) 223, 783
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 4 cut(s) 5, 890, 985, 1029
PinAI ACCGGT 2 cut(s) 1100, 1258
PkrI GCNGC 7 cut(s) 29, 64, 291, 376, 411, 475, 721
PleI GAGTC 4 cut(s) 153, 628, 1067, 1203
PpsI GAGTC 4 cut(s) 153, 628, 1067, 1203
Psp124BI GAGCTC 1 cut(s) 1023
Psp1406I AACGTT 2 cut(s) 35, 298
Psp6I CCWGG 2 cut(s) 792, 1053
PspFI CCCAGC 1 cut(s) 405
PspGI CCWGG 2 cut(s) 792, 1053
PspN4I GGNNCC 2 cut(s) 93, 94
PspOMI GGGCCC 1 cut(s) 92
PspPI GGNCC 3 cut(s) 92, 93, 1307
PspXI VCTCGAGB 1 cut(s) 669
PstI CTGCAG 2 cut(s) 67, 294
PsuI RGATCY 2 cut(s) 108, 394
PvuII CAGCTG 2 cut(s) 409, 1184
RsaI GTAC 2 cut(s) 181, 327
RsaNI GTAC 2 cut(s) 180, 326
RseI CAYNNNNRTG 3 cut(s) 971, 1083, 1292
SacI GAGCTC 1 cut(s) 1023
SalI GTCGAC 1 cut(s) 864
SapI GCTCTTC 1 cut(s) 1011
SaqAI TTAA 4 cut(s) 878, 1005, 1043, 1290
SatI GCNGC 7 cut(s) 28, 63, 290, 375, 410, 474, 720
Sau3AI GATC 5 cut(s) 97, 108, 307, 394, 562
Sau96I GGNCC 3 cut(s) 92, 93, 1307
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 4 cut(s) 153, 629, 1067, 1203
ScrFI CCNGG 4 cut(s) 10, 277, 794, 1055
SduI GDGCHC 4 cut(s) 96, 538, 978, 1023
SfaNI GCATC 6 cut(s) 26, 361, 460, 485, 683, 813
SfcI CTRYAG 3 cut(s) 63, 290, 837
Sfr274I CTCGAG 2 cut(s) 669, 812
SlaI CTCGAG 2 cut(s) 669, 812
SmiMI CAYNNNNRTG 3 cut(s) 971, 1083, 1292
SmlI CTYRAG 6 cut(s) 347, 419, 669, 812, 1004, 1224
SmoI CTYRAG 6 cut(s) 347, 419, 669, 812, 1004, 1224
Sse9I AATT 8 cut(s) 45, 244, 546, 582, 650, 701, 851, 934
SsiI CCGC 2 cut(s) 28, 1321
SspMI CTAG 3 cut(s) 23, 729, 942
SstI GAGCTC 1 cut(s) 1023
StyD4I CCNGG 4 cut(s) 8, 275, 792, 1053
StyI CCWWGG 1 cut(s) 1059
TaaI ACNGT 7 cut(s) 286, 325, 330, 453, 863, 1001, 1318
TaiI ACGT 3 cut(s) 38, 301, 430
TaqI TCGA 5 cut(s) 157, 457, 670, 813, 865
TasI AATT 8 cut(s) 45, 244, 546, 582, 650, 701, 851, 934
TatI WGTACW 1 cut(s) 179
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 4 cut(s) 5, 890, 985, 1029
Tru1I TTAA 4 cut(s) 878, 1005, 1043, 1290
Tru9I TTAA 4 cut(s) 878, 1005, 1043, 1290
TscAI CASTG 2 cut(s) 983, 1323
TseFI GTSAC 1 cut(s) 1312
TseI GCWGC 6 cut(s) 62, 289, 374, 409, 473, 719
Tsp45I GTSAC 1 cut(s) 1312
TspDTI ATGAA 5 cut(s) 533, 765, 776, 1029, 1310
TspRI CASTG 2 cut(s) 983, 1323
Vha464I CTTAAG 1 cut(s) 1004
VneI GTGCAC 2 cut(s) 534, 974
XapI RAATTY 2 cut(s) 45, 244
XbaI TCTAGA 1 cut(s) 728
XhoI CTCGAG 2 cut(s) 669, 812
XmiI GTMKAC 1 cut(s) 865
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 3 cut(s) 23, 729, 942
ZrmI AGTACT 1 cut(s) 181
Zsp2I ATGCAT 2 cut(s) 785, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.