RchiOBHm_Chr6g0277921

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007556
Physical Location & Seq
Reverse (-)
40626760 .. 40628733
1974 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24933

Sequence Viewer

Length: 1365 bp
ATGGAGGTGACTATAATTTCCAGAGATAGCATCACACCAGCTGTTACTTCCTCTTTAGTTCATCGCCATAAGAAACCTTACCAACTTTCCATCTTTGATCAGCTCTTTCCCAAAACTTACATTCCACTGGTTTTGTTCTACCCCATTACTGACCCAAATATCAATGTTTCCCAAACCCTAGCCCACCTAAAAAACTCCCTATCATATACACTCGTTGTGTACTACCCATTTTCCGGACGGATAAAAAACAACCTTTATATTGATGATTTTGATGCGGGTGTCCCCTTCCTTGAAGCCCGAATCAATTGTCGCATGTCTGAGTTTCTTAAGCTCCATGAAATTGAGCTACTCAATCAGTTCATTCCATTCCATCCATTTTGTCAGGAAGCCAAATCTCAAGATGATCTGCTACCCTTATTAGCAATTCAAGTCAGTGTTTTTTCATGTGGTGGAATAGCAATTGGGATCTCCGCATCTCACAAGATCTTTGATGGGGCCACCGGAAACAATTTCCTCAAATCTTGGGCTGCTGTTTTTCGTGGGGATCATGATAAAACTCTGCTACACACTAATCTCTACCAAGCTTCCTTAAGTTTTCCAGCAAAAGATGACATACCAGAAAAATATCTGGCTTTCATGGATAACATCGTTTTCAGTGGAAAACATTATTTGATAAGGAGATTTGTGTTTAATGCTAAAGCCATAGACAATCTACGAGATAAAGCAAAGAGCGAGGTCGTGCCCAATCCAACACGTATAGAGACGCTAACTTGTTTTCTTTGGAAACACGCTACAGCTGCTTCTAGATATGGTAGTACTTCACGAAGAGCATCCATAATAGCGCATGTAGTGGATATAAGGTCGCAATTGAAGCTCTCGATGTTAGATAACGCCATTGGAAATATTTTTTGGTGGGCAATAGCGGCTAACATCCCTACAAATAAGGGAGAAACTGGTGATGATGATCAGTTGTGTGATCTGATGAAGCTTTTACATGAATCTATCAAAGGATTGGACAATGACTATTTGAAGACTTTCCAAGGAGAAGAGGGATTTGGAGCCATTTCTAAGTTCATCGATCGCCATTTAGAGGTTGTGTATTCTTTGAAACCGGAAACATATATATTCACCAGCTGGACTAAGATTTTCAAGGAAGTTGATTTTGGATGGGGGAGACCGTTTTGGATTGGGGTCATGGGGAAAGTTGGACCAACTGCGGGCAATGCCATAATGTTTGTTGAAACACAACAGGGTAAGGGCATAGAAGCATGGGTGACCTTGGAGGAAAAGCAAATGGCTGTAATGGAAAAAGATCCTCAATTTTTAGCATTTACTTCTCAAAACCCACGCATTTCAAGTATGTGA

Protein Analysis

454

Amino Acids

51.4

Weight (kDa)

6.9

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 441 6.5e-74 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 233
AciI CCGC 4 cut(s) 275, 471, 923, 1217
AclWI GGATC 3 cut(s) 473, 552, 1307
AfaI GTAC 2 cut(s) 221, 817
AfiI CCNNNNNNNGG 3 cut(s) 233, 1090, 1217
AflII CTTAAG 2 cut(s) 326, 589
AflIII ACRYGT 1 cut(s) 752
AgsI TTSAA 8 cut(s) 293, 428, 871, 1030, 1108, 1150, 1241, 1356
AjuI GAANNNNNNNTTGG 6 cut(s) 892, 924, 1038, 1070, 1146, 1178
AluBI AGCT 9 cut(s) 41, 103, 331, 346, 584, 797, 874, 988, 1134
AluI AGCT 9 cut(s) 41, 103, 331, 346, 584, 797, 874, 988, 1134
Alw26I GTCTC 2 cut(s) 755, 1168
AlwI GGATC 3 cut(s) 473, 552, 1307
Aor13HI TCCGGA 1 cut(s) 233
AoxI GGCC 1 cut(s) 495
ApeKI GCWGC 2 cut(s) 527, 797
ArsI GACNNNNNNTTYG 2 cut(s) 292, 324
Asp700I GAANNNNTTC 1 cut(s) 1034
AspLEI GCGC 1 cut(s) 844
AspS9I GGNCC 2 cut(s) 495, 1208
AsuHPI GGTGA 4 cut(s) 19, 968, 1120, 1285
AvaII GGWCC 1 cut(s) 1208
BaeGI GKGCMC 1 cut(s) 744
BbsI GAAGAC 1 cut(s) 1037
BbvI GCAGC 2 cut(s) 514, 784
BccI CCATC 4 cut(s) 98, 378, 485, 1161
BclI TGATCA 2 cut(s) 97, 964
BcoDI GTCTC 2 cut(s) 755, 1168
BfaI CTAG 2 cut(s) 179, 804
BfmI CTRYAG 1 cut(s) 792
BfrI CTTAAG 2 cut(s) 326, 589
BglII AGATCT 1 cut(s) 483
BisI GCNGC 3 cut(s) 528, 798, 924
BlsI GCNGC 3 cut(s) 529, 799, 925
BmcAI AGTACT 1 cut(s) 817
Bme18I GGWCC 1 cut(s) 1208
BmgT120I GGNCC 2 cut(s) 495, 1208
BmiI GGNNCC 2 cut(s) 496, 1060
BmsI GCATC 4 cut(s) 39, 262, 482, 839
BpiI GAAGAC 1 cut(s) 1037
BpuEI CTTGAG 1 cut(s) 381
Bsa29I ATCGAT 1 cut(s) 1077
BsaAI YACGTR 1 cut(s) 755
BsaBI GATNNNNATC 1 cut(s) 963
BsaI GGTCTC 1 cut(s) 1168
BsaJI CCNNGG 2 cut(s) 1039, 1278
BsaWI WCCGGW 3 cut(s) 233, 500, 1111
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 1090, 1217
Bse1I ACTGG 2 cut(s) 132, 958
Bse3DI GCAATG 1 cut(s) 1228
Bse8I GATNNNNATC 1 cut(s) 963
BseAI TCCGGA 1 cut(s) 233
BseCI ATCGAT 1 cut(s) 1077
BseDI CCNNGG 2 cut(s) 1039, 1278
BseGI GGATG 4 cut(s) 370, 830, 930, 1172
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 3 cut(s) 233, 1090, 1217
BseMI GCAATG 1 cut(s) 1228
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 132, 958
BseSI GKGCMC 1 cut(s) 744
BseXI GCAGC 2 cut(s) 514, 784
Bsh1285I CGRYCG 1 cut(s) 1081
BshFI GGCC 1 cut(s) 497
BshVI ATCGAT 1 cut(s) 1077
BsiEI CGRYCG 1 cut(s) 1081
BsiSI CCGG 3 cut(s) 234, 501, 1112
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 3 cut(s) 233, 1090, 1217
BsmAI GTCTC 2 cut(s) 755, 1168
BsmBI CGTCTC 1 cut(s) 755
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 1 cut(s) 497
Bso31I GGTCTC 1 cut(s) 1168
Bsp1286I GDGCHC 1 cut(s) 744
Bsp13I TCCGGA 1 cut(s) 233
Bsp143I GATC 9 cut(s) 97, 403, 465, 483, 544, 964, 976, 1078, 1312
BspACI CCGC 4 cut(s) 275, 471, 923, 1217
BspANI GGCC 1 cut(s) 497
BspCNI CTCAG 1 cut(s) 310
BspDI ATCGAT 1 cut(s) 1077
BspEI TCCGGA 1 cut(s) 233
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 2 cut(s) 496, 1060
BspPI GGATC 3 cut(s) 473, 552, 1307
BspQI GCTCTTC 1 cut(s) 820
BspTI CTTAAG 2 cut(s) 326, 589
BspTNI GGTCTC 1 cut(s) 1168
BsrDI GCAATG 1 cut(s) 1228
BsrI ACTGG 2 cut(s) 132, 958
BssECI CCNNGG 2 cut(s) 1039, 1278
BssMI GATC 9 cut(s) 97, 403, 465, 483, 544, 964, 976, 1078, 1312
BssT1I CCWWGG 2 cut(s) 1039, 1278
Bst4CI ACNGT 1 cut(s) 1179
Bst6I CTCTTC 2 cut(s) 820, 1041
BstAFI CTTAAG 2 cut(s) 326, 589
BstBAI YACGTR 1 cut(s) 755
BstC8I GCNNGC 1 cut(s) 1219
BstDEI CTNAG 3 cut(s) 318, 1068, 1140
BstEII GGTNACC 1 cut(s) 1273
BstF5I GGATG 4 cut(s) 370, 830, 930, 1172
BstHHI GCGC 1 cut(s) 844
BstKTI GATC 9 cut(s) 100, 406, 468, 486, 547, 967, 979, 1081, 1315
BstMAI GTCTC 2 cut(s) 755, 1168
BstMBI GATC 9 cut(s) 97, 403, 465, 483, 544, 964, 976, 1078, 1312
BstMCI CGRYCG 1 cut(s) 1081
BstMWI GCNNNNNNNGC 4 cut(s) 797, 871, 923, 1223
BstNSI RCATGY 2 cut(s) 316, 848
BstPI GGTNACC 1 cut(s) 1273
BstSFI CTRYAG 1 cut(s) 792
BstSLI GKGCMC 1 cut(s) 744
BstV1I GCAGC 2 cut(s) 514, 784
BstV2I GAAGAC 1 cut(s) 1037
BstX2I RGATCY 3 cut(s) 465, 483, 1312
BstYI RGATCY 3 cut(s) 465, 483, 1312
Bsu15I ATCGAT 1 cut(s) 1077
BsuRI GGCC 1 cut(s) 497
BsuTUI ATCGAT 1 cut(s) 1077
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 4 cut(s) 370, 830, 930, 1172
BtsIMutI CAGTG 3 cut(s) 125, 439, 661
Cac8I GCNNGC 1 cut(s) 1219
CciI TCATGA 1 cut(s) 547
CfoI GCGC 1 cut(s) 844
Cfr13I GGNCC 2 cut(s) 495, 1208
ClaI ATCGAT 1 cut(s) 1077
CseI GACGC 1 cut(s) 772
Csp6I GTAC 2 cut(s) 220, 816
CspCI CAANNNNNGTGG 2 cut(s) 114, 149
CviAII CATG 9 cut(s) 313, 335, 444, 548, 637, 845, 995, 1195, 1269
CviQI GTAC 2 cut(s) 220, 816
DdeI CTNAG 3 cut(s) 318, 1068, 1140
DpnI GATC 9 cut(s) 99, 405, 467, 485, 546, 966, 978, 1080, 1314
DpnII GATC 9 cut(s) 97, 403, 465, 483, 544, 964, 976, 1078, 1312
Eam1104I CTCTTC 2 cut(s) 820, 1041
EarI CTCTTC 2 cut(s) 820, 1041
Eco130I CCWWGG 2 cut(s) 1039, 1278
Eco31I GGTCTC 1 cut(s) 1168
Eco47I GGWCC 1 cut(s) 1208
Eco91I GGTNACC 1 cut(s) 1273
EcoO65I GGTNACC 1 cut(s) 1273
EcoT14I CCWWGG 2 cut(s) 1039, 1278
ErhI CCWWGG 2 cut(s) 1039, 1278
Esp3I CGTCTC 1 cut(s) 755
FaeI CATG 9 cut(s) 316, 338, 447, 551, 640, 848, 998, 1198, 1272
FaqI GGGAC 1 cut(s) 266
FatI CATG 9 cut(s) 312, 334, 443, 547, 636, 844, 994, 1194, 1268
FauI CCCGC 2 cut(s) 268, 1210
FbaI TGATCA 2 cut(s) 97, 964
Fnu4HI GCNGC 3 cut(s) 528, 798, 924
FokI GGATG 4 cut(s) 357, 817, 917, 1179
Fsp4HI GCNGC 3 cut(s) 528, 798, 924
FspBI CTAG 2 cut(s) 179, 804
GlaI GCGC 1 cut(s) 843
GluI GCNGC 3 cut(s) 528, 798, 924
HaeIII GGCC 1 cut(s) 497
HapII CCGG 3 cut(s) 234, 501, 1112
HgaI GACGC 1 cut(s) 772
HhaI GCGC 1 cut(s) 844
Hin1II CATG 9 cut(s) 316, 338, 447, 551, 640, 848, 998, 1198, 1272
Hin6I GCGC 1 cut(s) 842
HinP1I GCGC 1 cut(s) 842
HindIII AAGCTT 2 cut(s) 582, 986
HinfI GANTC 2 cut(s) 300, 998
HpaII CCGG 3 cut(s) 234, 501, 1112
HphI GGTGA 4 cut(s) 19, 968, 1120, 1285
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 2 cut(s) 319, 981
Hpy188III TCNNGA 8 cut(s) 21, 234, 383, 398, 548, 804, 822, 877
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 1 cut(s) 1179
HpyCH4IV ACGT 1 cut(s) 754
HpyF10VI GCNNNNNNNGC 4 cut(s) 797, 871, 923, 1223
HpyF3I CTNAG 3 cut(s) 318, 1068, 1140
HpySE526I ACGT 1 cut(s) 754
Hsp92II CATG 9 cut(s) 316, 338, 447, 551, 640, 848, 998, 1198, 1272
HspAI GCGC 1 cut(s) 842
Kpn2I TCCGGA 1 cut(s) 233
Ksp22I TGATCA 2 cut(s) 97, 964
Kzo9I GATC 9 cut(s) 97, 403, 465, 483, 544, 964, 976, 1078, 1312
LguI GCTCTTC 1 cut(s) 820
LmnI GCTCC 2 cut(s) 336, 1058
Lsp1109I GCAGC 2 cut(s) 514, 784
LweI GCATC 4 cut(s) 39, 262, 482, 839
MaeI CTAG 2 cut(s) 179, 804
MaeII ACGT 1 cut(s) 754
MaeIII GTNAC 3 cut(s) 7, 43, 1273
MalI GATC 9 cut(s) 99, 405, 467, 485, 546, 966, 978, 1080, 1314
MboI GATC 9 cut(s) 97, 403, 465, 483, 544, 964, 976, 1078, 1312
MboII GAAGA 3 cut(s) 837, 1042, 1058
MfeI CAATTG 3 cut(s) 304, 459, 866
MflI RGATCY 3 cut(s) 465, 483, 1312
MhlI GDGCHC 1 cut(s) 744
MluCI AATT 8 cut(s) 15, 304, 339, 423, 459, 508, 866, 1319
MmeI TCCRAC 2 cut(s) 773, 1186
MnlI CCTC 7 cut(s) 61, 524, 727, 1042, 1084, 1276, 1326
MroI TCCGGA 1 cut(s) 233
MroXI GAANNNNTTC 1 cut(s) 1034
MseI TTAA 3 cut(s) 327, 590, 690
MspA1I CMGCKG 3 cut(s) 41, 797, 1134
MspCI CTTAAG 2 cut(s) 326, 589
MspI CCGG 3 cut(s) 234, 501, 1112
MunI CAATTG 3 cut(s) 304, 459, 866
MwoI GCNNNNNNNGC 4 cut(s) 797, 871, 923, 1223
NdeII GATC 9 cut(s) 97, 403, 465, 483, 544, 964, 976, 1078, 1312
NlaIII CATG 9 cut(s) 316, 338, 447, 551, 640, 848, 998, 1198, 1272
NlaIV GGNNCC 2 cut(s) 496, 1060
NmuCI GTSAC 2 cut(s) 7, 1273
NspI RCATGY 2 cut(s) 316, 848
PagI TCATGA 1 cut(s) 547
PciSI GCTCTTC 1 cut(s) 820
PdmI GAANNNNTTC 1 cut(s) 1034
PfeI GAWTC 2 cut(s) 300, 998
PkrI GCNGC 3 cut(s) 529, 799, 925
Ple19I CGATCG 1 cut(s) 1081
Ppu21I YACGTR 1 cut(s) 755
PspEI GGTNACC 1 cut(s) 1273
PspN4I GGNNCC 2 cut(s) 496, 1060
PspPI GGNCC 2 cut(s) 495, 1208
PsuI RGATCY 3 cut(s) 465, 483, 1312
PvuI CGATCG 1 cut(s) 1081
PvuII CAGCTG 3 cut(s) 41, 797, 1134
RsaI GTAC 2 cut(s) 221, 817
RsaNI GTAC 2 cut(s) 220, 816
SapI GCTCTTC 1 cut(s) 820
SaqAI TTAA 3 cut(s) 327, 590, 690
SatI GCNGC 3 cut(s) 528, 798, 924
Sau3AI GATC 9 cut(s) 97, 403, 465, 483, 544, 964, 976, 1078, 1312
Sau96I GGNCC 2 cut(s) 495, 1208
ScaI AGTACT 1 cut(s) 817
SduI GDGCHC 1 cut(s) 744
SfaNI GCATC 4 cut(s) 39, 262, 482, 839
SfcI CTRYAG 1 cut(s) 792
SinI GGWCC 1 cut(s) 1208
SmlI CTYRAG 3 cut(s) 326, 396, 589
SmoI CTYRAG 3 cut(s) 326, 396, 589
Sse9I AATT 8 cut(s) 15, 304, 339, 423, 459, 508, 866, 1319
SsiI CCGC 4 cut(s) 275, 471, 923, 1217
SspI AATATT 1 cut(s) 904
SspMI CTAG 2 cut(s) 179, 804
StyI CCWWGG 2 cut(s) 1039, 1278
TaaI ACNGT 1 cut(s) 1179
TaiI ACGT 1 cut(s) 757
TaqI TCGA 2 cut(s) 878, 1077
TasI AATT 8 cut(s) 15, 304, 339, 423, 459, 508, 866, 1319
TatI WGTACW 2 cut(s) 219, 815
TauI GCSGC 1 cut(s) 926
TfiI GAWTC 2 cut(s) 300, 998
Tru1I TTAA 3 cut(s) 327, 590, 690
Tru9I TTAA 3 cut(s) 327, 590, 690
TscAI CASTG 3 cut(s) 132, 439, 661
TseFI GTSAC 2 cut(s) 7, 1273
TseI GCWGC 2 cut(s) 527, 797
Tsp45I GTSAC 2 cut(s) 7, 1273
TspDTI ATGAA 8 cut(s) 50, 349, 351, 432, 625, 998, 1011, 1063
TspGWI ACGGA 1 cut(s) 253
TspRI CASTG 3 cut(s) 132, 439, 661
Vha464I CTTAAG 2 cut(s) 326, 589
VpaK11BI GGWCC 1 cut(s) 1208
XbaI TCTAGA 1 cut(s) 803
XceI RCATGY 2 cut(s) 316, 848
XmnI GAANNNNTTC 1 cut(s) 1034
XspI CTAG 2 cut(s) 179, 804
ZrmI AGTACT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.