RLG00000013256

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007556
Physical Location & Seq
Forward (+)
28727508 .. 28727993
486 bp
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UTR
Exon/CDS
Intron
RLM00000013256

Sequence Viewer

Length: 486 bp
ATGTTAGATAACACCATTGGAAATATGTTTTGGTGGGCAATAGCGGCTAACATCCCTACTAATAAGGGAGAAACTGGCGATGATGATAAGTTGTGTGATTTGATGAAGCTTTTACATGAATCTATCAAAGGATTAGACAATGACTATTTGAAGACATTGCAGGGAGAAGATGGATTTAGAGCCATTTCTAAGTTCATCGATCACCATTTAGAGGTTGTGTATTCTTTGAAACCGGAAACATATATATTCACAAGCTGGACTAAGATTTTCAAGGAAGTTGATTTTGGATGTGGGAGACCGTTTTGGATTGGGCTCATGGGGAAAGTTGGACCAACTATGGGCAATCTCACAGTGTTCGTTGAAACACAATGCGGTAAGGGCATAGAAGCATGGGTGACCTTGGAGGAAAAGCAAATGGCTGTAATGGAAAAAGATCCTCAATTTTTAGCATTTACTTCTCCAAACCCACGCATTTCAAGCATGTGA

Protein Analysis

162

Amino Acids

18.23

Weight (kDa)

5.04

Isoelectric Point (pI)

15.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 31 - 148 2e-11 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 44, 372
AclWI GGATC 1 cut(s) 428
AfiI CCNNNNNNNGG 2 cut(s) 211, 338
AgsI TTSAA 5 cut(s) 151, 229, 271, 362, 477
AjuI GAANNNNNNNTTGG 4 cut(s) 13, 45, 267, 299
AluBI AGCT 2 cut(s) 109, 255
AluI AGCT 2 cut(s) 109, 255
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 1 cut(s) 428
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 2 cut(s) 194, 406
AvaII GGWCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 158
BccI CCATC 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 289
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BpiI GAAGAC 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 1 cut(s) 399
BsaWI WCCGGW 1 cut(s) 232
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 338
Bse1I ACTGG 1 cut(s) 79
Bse3DI GCAATG 1 cut(s) 155
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 2 cut(s) 51, 293
BseLI CCNNNNNNNGG 2 cut(s) 211, 338
BseMI GCAATG 1 cut(s) 155
BseNI ACTGG 1 cut(s) 79
BshVI ATCGAT 1 cut(s) 198
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 211, 338
BsmAI GTCTC 1 cut(s) 289
Bso31I GGTCTC 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 2 cut(s) 199, 433
BspACI CCGC 2 cut(s) 44, 372
BspDI ATCGAT 1 cut(s) 198
BspPI GGATC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 289
BsrDI GCAATG 1 cut(s) 155
BsrI ACTGG 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 2 cut(s) 199, 433
BssT1I CCWWGG 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 300, 352
BstDEI CTNAG 2 cut(s) 189, 261
BstEII GGTNACC 1 cut(s) 394
BstF5I GGATG 2 cut(s) 51, 293
BstKTI GATC 2 cut(s) 202, 436
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 2 cut(s) 199, 433
BstMWI GCNNNNNNNGC 3 cut(s) 44, 378, 477
BstNSI RCATGY 1 cut(s) 484
BstPI GGTNACC 1 cut(s) 394
BstV2I GAAGAC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
Bsu15I ATCGAT 1 cut(s) 198
BsuTUI ATCGAT 1 cut(s) 198
BtgZI GCGATG 1 cut(s) 93
BtsCI GGATG 2 cut(s) 51, 293
BtsIMutI CAGTG 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 329
ClaI ATCGAT 1 cut(s) 198
CviAII CATG 4 cut(s) 116, 316, 390, 481
CviJI RGCY 6 cut(s) 47, 109, 182, 255, 313, 419
CviKI_1 RGCY 6 cut(s) 47, 109, 182, 255, 313, 419
DdeI CTNAG 2 cut(s) 189, 261
DpnI GATC 2 cut(s) 201, 435
DpnII GATC 2 cut(s) 199, 433
Eco130I CCWWGG 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 329
Eco91I GGTNACC 1 cut(s) 394
EcoO65I GGTNACC 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 4 cut(s) 119, 319, 393, 484
FatI CATG 4 cut(s) 115, 315, 389, 480
Fnu4HI GCNGC 1 cut(s) 45
FokI GGATG 2 cut(s) 38, 300
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 1 cut(s) 45
GluI GCNGC 1 cut(s) 45
HapII CCGG 1 cut(s) 233
Hin1II CATG 4 cut(s) 119, 319, 393, 484
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 1 cut(s) 119
HpaII CCGG 1 cut(s) 233
HphI GGTGA 2 cut(s) 194, 406
HpyCH4III ACNGT 2 cut(s) 300, 352
HpyCH4V TGCA 1 cut(s) 160
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 378, 477
HpyF3I CTNAG 2 cut(s) 189, 261
Hsp92II CATG 4 cut(s) 119, 319, 393, 484
Kzo9I GATC 2 cut(s) 199, 433
LpnPI CCDG 4 cut(s) 60, 146, 241, 246
MaeIII GTNAC 1 cut(s) 394
MalI GATC 2 cut(s) 201, 435
MboI GATC 2 cut(s) 199, 433
MboII GAAGA 2 cut(s) 163, 179
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 3 cut(s) 205, 397, 447
MspI CCGG 1 cut(s) 233
MwoI GCNNNNNNNGC 3 cut(s) 44, 378, 477
NdeII GATC 2 cut(s) 199, 433
NlaIII CATG 4 cut(s) 119, 319, 393, 484
NmuCI GTSAC 1 cut(s) 394
NspI RCATGY 1 cut(s) 484
PfeI GAWTC 1 cut(s) 119
PkrI GCNGC 1 cut(s) 46
PspEI GGTNACC 1 cut(s) 394
PspPI GGNCC 1 cut(s) 329
PsuI RGATCY 1 cut(s) 433
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 2 cut(s) 199, 433
Sau96I GGNCC 1 cut(s) 329
SduI GDGCHC 1 cut(s) 315
SetI ASST 4 cut(s) 111, 216, 257, 401
SinI GGWCC 1 cut(s) 329
Sse9I AATT 1 cut(s) 440
SsiI CCGC 2 cut(s) 44, 372
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 300, 352
TaqI TCGA 1 cut(s) 198
TasI AATT 1 cut(s) 440
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 1 cut(s) 119
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 1 cut(s) 394
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 3 cut(s) 119, 132, 184
TspRI CASTG 1 cut(s) 357
VpaK11BI GGWCC 1 cut(s) 329
XceI RCATGY 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.