RchiOBHm_Chr7g0198771

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007556
Physical Location & Seq
Forward (+)
16776379 .. 16776864
486 bp
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UTR
Exon/CDS
Intron
PRQ17785

Sequence Viewer

Length: 486 bp
ATGTCTGATAATGCCACTGGAAATATTTTTTGGTGGGCAACAGTAGCCAGCATCCCTACTGATGAGGGACAAACTAGTGATAACGATAAGTTGTGTGATCCAATGAAGCTTGTACGTGAATCTATCAAAGGATTTGACAATGAGTGTGAAGACATTGCAGGGAGGAGGGATTTGGAGCCATTTCTGAGTTCTTCGATCGGCAGCAAGAGGTTGCGCATGCATTCTTTAGAAGTGGAAACATATGCATTCACCAGCTGGACTAAGATTCTTAATGAAGTTCATTTTGGATGGGGAAGGCCGTGTTGGATTGGGGTCATGGGGAAAGTTGGACCAACATTTAGGAATCTCACAGTGTTCCTTGAAACACAATGGGGTAAGGGCATTGAAGCATGGGTTACCTCGGAGGAAAAGCAAATAGTTATAATGGAAAAGGAACCTCAGTTTTTAGCATTTGCGTCTCCAAACCTACACATTTTAAGCATGTAA

Protein Analysis

161

Amino Acids

18.28

Weight (kDa)

5.08

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 77 - 148 9.1e-07 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 422
Acc16I TGCGCA 1 cut(s) 215
AclWI GGATC 1 cut(s) 92
AfaI GTAC 1 cut(s) 114
AgsI TTSAA 2 cut(s) 362, 386
AhlI ACTAGT 1 cut(s) 74
AjuI GAANNNNNNNTTGG 6 cut(s) 13, 45, 267, 286, 299, 318
AluBI AGCT 2 cut(s) 109, 255
AluI AGCT 2 cut(s) 109, 255
Alw26I GTCTC 1 cut(s) 462
AlwI GGATC 1 cut(s) 92
AoxI GGCC 1 cut(s) 296
ApeKI GCWGC 1 cut(s) 201
AspLEI GCGC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 1 cut(s) 241
AvaII GGWCC 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 156
BbvI GCAGC 1 cut(s) 213
BccI CCATC 1 cut(s) 282
BceAI ACGGC 1 cut(s) 283
BcoDI GTCTC 1 cut(s) 462
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 1 cut(s) 75
BisI GCNGC 1 cut(s) 202
BlsI GCNGC 1 cut(s) 203
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 2 cut(s) 177, 435
BmsI GCATC 1 cut(s) 60
BpiI GAAGAC 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 2 cut(s) 51, 293
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 2 cut(s) 176, 452
BseNI ACTGG 1 cut(s) 22
BseRI GAGGAG 1 cut(s) 178
BseXI GCAGC 1 cut(s) 213
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 1 cut(s) 298
BsiEI CGRYCG 1 cut(s) 198
BslFI GGGAC 1 cut(s) 81
BsmAI GTCTC 1 cut(s) 462
BsmBI CGTCTC 1 cut(s) 462
BsmFI GGGAC 1 cut(s) 81
BsmI GAATGC 2 cut(s) 220, 245
BsnI GGCC 1 cut(s) 298
Bsp143I GATC 2 cut(s) 97, 195
BspANI GGCC 1 cut(s) 298
BspCNI CTCAG 2 cut(s) 177, 451
BspLI GGNNCC 2 cut(s) 177, 435
BspPI GGATC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 153
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 2 cut(s) 97, 195
Bst4CI ACNGT 2 cut(s) 43, 352
BstBAI YACGTR 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 49, 218
BstDEI CTNAG 3 cut(s) 185, 261, 438
BstEII GGTNACC 1 cut(s) 394
BstF5I GGATG 2 cut(s) 51, 293
BstHHI GCGC 1 cut(s) 216
BstKTI GATC 2 cut(s) 100, 198
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 2 cut(s) 97, 195
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNSI RCATGY 2 cut(s) 220, 484
BstPI GGTNACC 1 cut(s) 394
BstV1I GCAGC 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 156
BsuRI GGCC 1 cut(s) 298
BtsCI GGATG 2 cut(s) 51, 293
BtsIMutI CAGTG 2 cut(s) 15, 357
Cac8I GCNNGC 2 cut(s) 49, 218
CfoI GCGC 1 cut(s) 216
Cfr13I GGNCC 1 cut(s) 329
CseI GACGC 1 cut(s) 444
Csp6I GTAC 1 cut(s) 113
CviAII CATG 4 cut(s) 217, 316, 390, 481
CviJI RGCY 5 cut(s) 47, 109, 178, 255, 298
CviKI_1 RGCY 5 cut(s) 47, 109, 178, 255, 298
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 3 cut(s) 185, 261, 438
DpnI GATC 2 cut(s) 99, 197
DpnII GATC 2 cut(s) 97, 195
Eco47I GGWCC 1 cut(s) 329
Eco91I GGTNACC 1 cut(s) 394
EcoO65I GGTNACC 1 cut(s) 394
EcoT22I ATGCAT 2 cut(s) 222, 247
Esp3I CGTCTC 1 cut(s) 462
FaeI CATG 4 cut(s) 220, 319, 393, 484
FaiI YATR 7 cut(s) 218, 241, 243, 317, 391, 422, 482
FaqI GGGAC 1 cut(s) 81
FatI CATG 4 cut(s) 216, 315, 389, 480
FauNDI CATATG 1 cut(s) 241
Fnu4HI GCNGC 1 cut(s) 202
FokI GGATG 2 cut(s) 38, 300
Fsp4HI GCNGC 1 cut(s) 202
FspBI CTAG 1 cut(s) 75
FspI TGCGCA 1 cut(s) 215
GlaI GCGC 1 cut(s) 215
GluI GCNGC 1 cut(s) 202
HaeIII GGCC 1 cut(s) 298
HgaI GACGC 1 cut(s) 444
HhaI GCGC 1 cut(s) 216
Hin1II CATG 4 cut(s) 220, 319, 393, 484
Hin6I GCGC 1 cut(s) 214
HinP1I GCGC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 3 cut(s) 119, 265, 343
HphI GGTGA 1 cut(s) 241
Hpy188I TCNGA 3 cut(s) 7, 186, 403
HpyAV CCTTC 1 cut(s) 288
HpyCH4III ACNGT 2 cut(s) 43, 352
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 3 cut(s) 158, 220, 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 3 cut(s) 185, 261, 438
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 4 cut(s) 220, 319, 393, 484
HspAI GCGC 1 cut(s) 214
Kzo9I GATC 2 cut(s) 97, 195
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 5 cut(s) 3, 61, 144, 241, 265
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 1 cut(s) 60
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 1 cut(s) 394
MalI GATC 2 cut(s) 99, 197
MboI GATC 2 cut(s) 97, 195
MboII GAAGA 2 cut(s) 161, 183
MmeI TCCRAC 2 cut(s) 284, 307
MnlI CCTC 7 cut(s) 58, 156, 159, 201, 397, 409, 447
Mph1103I ATGCAT 2 cut(s) 222, 247
MseI TTAA 2 cut(s) 270, 476
MspA1I CMGCKG 1 cut(s) 255
Mva1269I GAATGC 2 cut(s) 220, 245
MwoI GCNNNNNNNGC 1 cut(s) 44
NdeI CATATG 1 cut(s) 241
NdeII GATC 2 cut(s) 97, 195
NlaIII CATG 4 cut(s) 220, 319, 393, 484
NlaIV GGNNCC 2 cut(s) 177, 435
NsbI TGCGCA 1 cut(s) 215
NsiI ATGCAT 2 cut(s) 222, 247
NspI RCATGY 2 cut(s) 220, 484
PaeI GCATGC 1 cut(s) 220
PctI GAATGC 2 cut(s) 220, 245
PfeI GAWTC 3 cut(s) 119, 265, 343
PkrI GCNGC 1 cut(s) 203
Ple19I CGATCG 1 cut(s) 198
Ppu21I YACGTR 1 cut(s) 116
PsiI TTATAA 1 cut(s) 422
PspEI GGTNACC 1 cut(s) 394
PspN4I GGNNCC 2 cut(s) 177, 435
PspPI GGNCC 1 cut(s) 329
PvuI CGATCG 1 cut(s) 198
PvuII CAGCTG 1 cut(s) 255
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 270, 476
SatI GCNGC 1 cut(s) 202
Sau3AI GATC 2 cut(s) 97, 195
Sau96I GGNCC 1 cut(s) 329
SetI ASST 7 cut(s) 111, 118, 212, 257, 401, 439, 468
SfaNI GCATC 1 cut(s) 60
SinI GGWCC 1 cut(s) 329
SpeI ACTAGT 1 cut(s) 74
SphI GCATGC 1 cut(s) 220
SspI AATATT 1 cut(s) 25
SspMI CTAG 1 cut(s) 75
TaaI ACNGT 2 cut(s) 43, 352
TaiI ACGT 1 cut(s) 118
TaqI TCGA 1 cut(s) 194
TfiI GAWTC 3 cut(s) 119, 265, 343
Tru1I TTAA 2 cut(s) 270, 476
Tru9I TTAA 2 cut(s) 270, 476
TscAI CASTG 2 cut(s) 22, 357
TseI GCWGC 1 cut(s) 201
TspDTI ATGAA 3 cut(s) 119, 269, 288
TspRI CASTG 2 cut(s) 22, 357
VpaK11BI GGWCC 1 cut(s) 329
XceI RCATGY 2 cut(s) 220, 484
XspI CTAG 1 cut(s) 75
Zsp2I ATGCAT 2 cut(s) 222, 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.