Rh6BG253100

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0007556
Physical Location & Seq
Reverse (-)
46454092 .. 46456374
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG253100.1

Sequence Viewer

Length: 1128 bp
ATGGGACGGACAAAAAACAACTTTTGTATTGATGATTTCGATGCAGGTGTCCCCTACCTTGAAGCCCGAATCAATTATCGCTTGTTTGAGTTTCTTAGGCTCCATGAAATCGAGCTACTCAATCAGTTCGTTCCATTCCATCCACTTCGCCAGGAAACCGAATCTCATGATGATCTGCTGCCCTTATTAGCAGTTCAAGTCAGTGTTTTTTCTTGTGGTGGAATAGCAATTGGAGTCTCCCTATCTCACAAGATCTTTGATGGGGACACGGCAAACAATTTCCTTAAATCTTGGGCTGTTGTTTTTCGTGGGGATCATGATAAAATTCTGCTGCACACTAATCTCTACCAAGCTTCATTAAGTTTTCCAGCAAAAGATGACATACCAGAAAAATATATGTCTTTCACAGGTAGCTTGGTATTCGGGGGAAAAAATTATATGACAAGGAGATTTATATTTAATGCTAAAGCCATAGACATTCTACGAGACAAAGCAAAGAGCGAGGTGGTGCCCAATACAACACGTATAGAGACGCTAACTTGTTTTCTTTGGAAACACGCTACAGCTGCTTCTAGATATGGTAGTACTTCACGAAGAGCATCAATAGCAGTGCATGCAGTGAACATAAGGCCGCGATTGAAGCCCTCAATGTTAGATAACGCCATTGGAAATATTATTTGGTGGTCAACAGCAACTAACATCCCTACTAATAAGAAAGAAACTGGTGATGATGATCAGTTATGTGATTTGATGAAGCTTTTACATGAATCTATCAAAAGATTGGACAAAGACTATTTGAAGACATTGCAGGGAGAAGAAGGATTTGGAGCCATTTCTGAGTTCATCGATCGCCAAATAGAGGTTGCGTATTCTTTGAAACCGGAAATATATATATTCACCAGCTGGACTAAGATTTTCAAGGAAGTTGATTTTGGATGGGGGAGACCGTTTTGGATTGGGACCATGGGGAAAGTAGGACCAACTCTTGGCAATCTCACAGTGTTCGTTGAAACCCCATTAGGCATAGAAGCATGGGTGACCTTGGAGGAAAAGCAAATGGCTGTAATGGAAAAAGATCCTCAGTTTTTAGCATTTACTTCTCCAAACCCACGCATTTCAAGCATGTGA

Protein Analysis

375

Amino Acids

42.62

Weight (kDa)

6.52

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 13 - 362 2.2e-52 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 35
Acc36I ACCTGC 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 508
AccB7I CCANNNNNTGG 1 cut(s) 986
AccII CGCG 1 cut(s) 634
AciI CCGC 1 cut(s) 632
AclWI GGATC 2 cut(s) 321, 1070
AcsI RAATTY 1 cut(s) 324
AfaI GTAC 1 cut(s) 586
AfiI CCNNNNNNNGG 2 cut(s) 859, 986
AflIII ACRYGT 1 cut(s) 521
AgsI TTSAA 8 cut(s) 62, 197, 640, 799, 877, 919, 1010, 1119
AjnI CCWGG 1 cut(s) 150
AjuI GAANNNNNNNTTGG 6 cut(s) 661, 693, 807, 839, 915, 947
AluBI AGCT 6 cut(s) 115, 353, 414, 566, 757, 903
AluI AGCT 6 cut(s) 115, 353, 414, 566, 757, 903
Alw26I GTCTC 4 cut(s) 241, 480, 524, 937
AlwI GGATC 2 cut(s) 321, 1070
AoxI GGCC 1 cut(s) 629
ApeKI GCWGC 3 cut(s) 178, 331, 566
ApoI RAATTY 1 cut(s) 324
AspS9I GGNCC 2 cut(s) 960, 977
AsuHPI GGTGA 3 cut(s) 737, 889, 1048
AvaII GGWCC 2 cut(s) 960, 977
BaeGI GKGCMC 1 cut(s) 513
BanI GGYRCC 1 cut(s) 508
BbsI GAAGAC 1 cut(s) 806
BbvI GCAGC 3 cut(s) 165, 318, 553
BccI CCATC 3 cut(s) 147, 254, 930
BceAI ACGGC 1 cut(s) 285
BciT130I CCWGG 1 cut(s) 152
BclI TGATCA 1 cut(s) 733
BcoDI GTCTC 4 cut(s) 241, 480, 524, 937
BfaI CTAG 1 cut(s) 573
BfmI CTRYAG 1 cut(s) 561
BfuAI ACCTGC 1 cut(s) 35
BglII AGATCT 1 cut(s) 252
BisI GCNGC 4 cut(s) 179, 332, 567, 632
BlsI GCNGC 4 cut(s) 180, 333, 568, 633
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 2 cut(s) 960, 977
BmgT120I GGNCC 2 cut(s) 960, 977
BmiI GGNNCC 4 cut(s) 101, 510, 829, 961
BmrFI CCNGG 1 cut(s) 152
BmsI GCATC 2 cut(s) 31, 608
BpiI GAAGAC 1 cut(s) 806
Bsa29I ATCGAT 1 cut(s) 846
BsaAI YACGTR 1 cut(s) 524
BsaBI GATNNNNATC 1 cut(s) 732
BsaI GGTCTC 1 cut(s) 937
BsaJI CCNNGG 2 cut(s) 963, 1041
BsaWI WCCGGW 1 cut(s) 880
Bsc4I CCNNNNNNNGG 2 cut(s) 859, 986
Bse1I ACTGG 1 cut(s) 727
Bse3DI GCAATG 1 cut(s) 803
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 1 cut(s) 152
BseCI ATCGAT 1 cut(s) 846
BseDI CCNNGG 2 cut(s) 963, 1041
BseGI GGATG 3 cut(s) 139, 699, 941
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 2 cut(s) 859, 986
BseMI GCAATG 1 cut(s) 803
BseMII CTCAG 2 cut(s) 828, 1094
BseNI ACTGG 1 cut(s) 727
BseSI GKGCMC 1 cut(s) 513
BseXI GCAGC 3 cut(s) 165, 318, 553
BsgI GTGCAG 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 634
Bsh1285I CGRYCG 1 cut(s) 850
BshFI GGCC 1 cut(s) 631
BshNI GGYRCC 1 cut(s) 508
BshVI ATCGAT 1 cut(s) 846
BsiEI CGRYCG 1 cut(s) 850
BsiSI CCGG 1 cut(s) 881
BslFI GGGAC 4 cut(s) 18, 35, 278, 973
BslI CCNNNNNNNGG 2 cut(s) 859, 986
BsmAI GTCTC 4 cut(s) 241, 480, 524, 937
BsmBI CGTCTC 1 cut(s) 524
BsmFI GGGAC 4 cut(s) 18, 35, 278, 973
BsnI GGCC 1 cut(s) 631
Bso31I GGTCTC 1 cut(s) 937
Bsp1286I GDGCHC 1 cut(s) 513
Bsp143I GATC 6 cut(s) 172, 252, 313, 733, 847, 1075
Bsp19I CCATGG 1 cut(s) 963
BspACI CCGC 1 cut(s) 632
BspANI GGCC 1 cut(s) 631
BspCNI CTCAG 2 cut(s) 829, 1093
BspDI ATCGAT 1 cut(s) 846
BspFNI CGCG 1 cut(s) 634
BspHI TCATGA 2 cut(s) 166, 316
BspLI GGNNCC 4 cut(s) 101, 510, 829, 961
BspMI ACCTGC 1 cut(s) 35
BspPI GGATC 2 cut(s) 321, 1070
BspQI GCTCTTC 1 cut(s) 589
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 1 cut(s) 937
BsrDI GCAATG 1 cut(s) 803
BsrI ACTGG 1 cut(s) 727
BssECI CCNNGG 2 cut(s) 963, 1041
BssMI GATC 6 cut(s) 172, 252, 313, 733, 847, 1075
BssT1I CCWWGG 2 cut(s) 963, 1041
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 2 cut(s) 948, 1000
Bst6I CTCTTC 1 cut(s) 589
BstAPI GCANNNNNTGC 1 cut(s) 614
BstBAI YACGTR 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 615
BstDEI CTNAG 4 cut(s) 95, 837, 909, 1080
BstDSI CCRYGG 1 cut(s) 963
BstEII GGTNACC 1 cut(s) 1036
BstF5I GGATG 3 cut(s) 139, 699, 941
BstFNI CGCG 1 cut(s) 634
BstKTI GATC 6 cut(s) 175, 255, 316, 736, 850, 1078
BstMAI GTCTC 4 cut(s) 241, 480, 524, 937
BstMBI GATC 6 cut(s) 172, 252, 313, 733, 847, 1075
BstMCI CGRYCG 1 cut(s) 850
BstMWI GCNNNNNNNGC 5 cut(s) 566, 605, 614, 640, 1119
BstNI CCWGG 1 cut(s) 152
BstNSI RCATGY 2 cut(s) 617, 1126
BstPI GGTNACC 1 cut(s) 1036
BstSCI CCNGG 1 cut(s) 150
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 513
BstUI CGCG 1 cut(s) 634
BstV1I GCAGC 3 cut(s) 165, 318, 553
BstV2I GAAGAC 1 cut(s) 806
BstX2I RGATCY 2 cut(s) 252, 1075
BstYI RGATCY 2 cut(s) 252, 1075
Bsu15I ATCGAT 1 cut(s) 846
BsuRI GGCC 1 cut(s) 631
BsuTUI ATCGAT 1 cut(s) 846
BtgI CCRYGG 1 cut(s) 963
BtsCI GGATG 3 cut(s) 139, 699, 941
BtsI GCAGTG 2 cut(s) 615, 624
BtsIMutI CAGTG 4 cut(s) 208, 615, 624, 1005
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 1 cut(s) 615
CciI TCATGA 2 cut(s) 166, 316
Cfr13I GGNCC 2 cut(s) 960, 977
ClaI ATCGAT 1 cut(s) 846
CseI GACGC 1 cut(s) 541
Csp6I GTAC 1 cut(s) 585
CviAII CATG 8 cut(s) 104, 167, 317, 614, 764, 964, 1032, 1123
CviQI GTAC 1 cut(s) 585
DdeI CTNAG 4 cut(s) 95, 837, 909, 1080
DpnI GATC 6 cut(s) 174, 254, 315, 735, 849, 1077
DpnII GATC 6 cut(s) 172, 252, 313, 733, 847, 1075
Eam1104I CTCTTC 1 cut(s) 589
EarI CTCTTC 1 cut(s) 589
Eco130I CCWWGG 2 cut(s) 963, 1041
Eco31I GGTCTC 1 cut(s) 937
Eco47I GGWCC 2 cut(s) 960, 977
Eco91I GGTNACC 1 cut(s) 1036
EcoO65I GGTNACC 1 cut(s) 1036
EcoRII CCWGG 1 cut(s) 150
EcoT14I CCWWGG 2 cut(s) 963, 1041
ErhI CCWWGG 2 cut(s) 963, 1041
Esp3I CGTCTC 1 cut(s) 524
FaeI CATG 8 cut(s) 107, 170, 320, 617, 767, 967, 1035, 1126
FaqI GGGAC 4 cut(s) 18, 35, 278, 973
FatI CATG 8 cut(s) 103, 166, 316, 613, 763, 963, 1031, 1122
FbaI TGATCA 1 cut(s) 733
Fnu4HI GCNGC 4 cut(s) 179, 332, 567, 632
FokI GGATG 3 cut(s) 126, 686, 948
Fsp4HI GCNGC 4 cut(s) 179, 332, 567, 632
FspBI CTAG 1 cut(s) 573
GluI GCNGC 4 cut(s) 179, 332, 567, 632
HaeIII GGCC 1 cut(s) 631
HapII CCGG 1 cut(s) 881
HgaI GACGC 1 cut(s) 541
Hin1II CATG 8 cut(s) 107, 170, 320, 617, 767, 967, 1035, 1126
HincII GTYRAC 1 cut(s) 687
HindII GTYRAC 1 cut(s) 687
HindIII AAGCTT 2 cut(s) 351, 755
HinfI GANTC 4 cut(s) 69, 161, 234, 767
HpaII CCGG 1 cut(s) 881
HphI GGTGA 3 cut(s) 737, 889, 1048
Hpy166II GTNNAC 2 cut(s) 622, 687
Hpy188I TCNGA 1 cut(s) 838
Hpy188III TCNNGA 4 cut(s) 167, 317, 573, 591
Hpy8I GTNNAC 2 cut(s) 622, 687
HpyAV CCTTC 1 cut(s) 812
HpyCH4III ACNGT 2 cut(s) 948, 1000
HpyCH4IV ACGT 1 cut(s) 523
HpyCH4V TGCA 5 cut(s) 44, 334, 613, 617, 808
HpyF10VI GCNNNNNNNGC 5 cut(s) 566, 605, 614, 640, 1119
HpyF3I CTNAG 4 cut(s) 95, 837, 909, 1080
HpySE526I ACGT 1 cut(s) 523
Hsp92II CATG 8 cut(s) 107, 170, 320, 617, 767, 967, 1035, 1126
Ksp22I TGATCA 1 cut(s) 733
Kzo9I GATC 6 cut(s) 172, 252, 313, 733, 847, 1075
LguI GCTCTTC 1 cut(s) 589
LmnI GCTCC 2 cut(s) 105, 827
Lsp1109I GCAGC 3 cut(s) 165, 318, 553
LweI GCATC 2 cut(s) 31, 608
MaeI CTAG 1 cut(s) 573
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 1 cut(s) 1036
MalI GATC 6 cut(s) 174, 254, 315, 735, 849, 1077
MboI GATC 6 cut(s) 172, 252, 313, 733, 847, 1075
MboII GAAGA 3 cut(s) 606, 811, 827
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 2 cut(s) 252, 1075
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 5 cut(s) 73, 228, 277, 324, 433
MlyI GAGTC 1 cut(s) 243
MnlI CCTC 5 cut(s) 496, 655, 853, 1039, 1089
MseI TTAA 3 cut(s) 285, 359, 459
MspA1I CMGCKG 2 cut(s) 566, 903
MspI CCGG 1 cut(s) 881
MspR9I CCNGG 1 cut(s) 152
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 152
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 5 cut(s) 566, 605, 614, 640, 1119
NcoI CCATGG 1 cut(s) 963
NdeII GATC 6 cut(s) 172, 252, 313, 733, 847, 1075
NlaIII CATG 8 cut(s) 107, 170, 320, 617, 767, 967, 1035, 1126
NlaIV GGNNCC 4 cut(s) 101, 510, 829, 961
NmuCI GTSAC 1 cut(s) 1036
NspI RCATGY 2 cut(s) 617, 1126
PaeI GCATGC 1 cut(s) 617
PagI TCATGA 2 cut(s) 166, 316
PaqCI CACCTGC 1 cut(s) 35
PciSI GCTCTTC 1 cut(s) 589
PfeI GAWTC 3 cut(s) 69, 161, 767
PflMI CCANNNNNTGG 1 cut(s) 986
PkrI GCNGC 4 cut(s) 180, 333, 568, 633
Ple19I CGATCG 1 cut(s) 850
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
Ppu21I YACGTR 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 150
PspEI GGTNACC 1 cut(s) 1036
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 4 cut(s) 101, 510, 829, 961
PspPI GGNCC 2 cut(s) 960, 977
PsuI RGATCY 2 cut(s) 252, 1075
PvuI CGATCG 1 cut(s) 850
PvuII CAGCTG 2 cut(s) 566, 903
RsaI GTAC 1 cut(s) 586
RsaNI GTAC 1 cut(s) 585
SapI GCTCTTC 1 cut(s) 589
SaqAI TTAA 3 cut(s) 285, 359, 459
SatI GCNGC 4 cut(s) 179, 332, 567, 632
Sau3AI GATC 6 cut(s) 172, 252, 313, 733, 847, 1075
Sau96I GGNCC 2 cut(s) 960, 977
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 1 cut(s) 243
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 1 cut(s) 513
SfaNI GCATC 2 cut(s) 31, 608
SfcI CTRYAG 1 cut(s) 561
SinI GGWCC 2 cut(s) 960, 977
SphI GCATGC 1 cut(s) 617
Sse9I AATT 5 cut(s) 73, 228, 277, 324, 433
SsiI CCGC 1 cut(s) 632
SspI AATATT 1 cut(s) 673
SspMI CTAG 1 cut(s) 573
StyD4I CCNGG 1 cut(s) 150
StyI CCWWGG 2 cut(s) 963, 1041
TaaI ACNGT 2 cut(s) 948, 1000
TaiI ACGT 1 cut(s) 526
TaqI TCGA 3 cut(s) 39, 111, 846
TasI AATT 5 cut(s) 73, 228, 277, 324, 433
TatI WGTACW 1 cut(s) 584
TauI GCSGC 1 cut(s) 634
TfiI GAWTC 3 cut(s) 69, 161, 767
Tru1I TTAA 3 cut(s) 285, 359, 459
Tru9I TTAA 3 cut(s) 285, 359, 459
TscAI CASTG 4 cut(s) 208, 615, 624, 1005
TseFI GTSAC 1 cut(s) 1036
TseI GCWGC 3 cut(s) 178, 331, 566
Tsp45I GTSAC 1 cut(s) 1036
TspDTI ATGAA 5 cut(s) 120, 345, 767, 780, 832
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 4 cut(s) 208, 615, 624, 1005
Van91I CCANNNNNTGG 1 cut(s) 986
VpaK11BI GGWCC 2 cut(s) 960, 977
XapI RAATTY 1 cut(s) 324
XbaI TCTAGA 1 cut(s) 572
XceI RCATGY 2 cut(s) 617, 1126
XspI CTAG 1 cut(s) 573
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.