Rh6BG253200

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0007556
Physical Location & Seq
Reverse (-)
46484133 .. 46484612
480 bp
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UTR
Exon/CDS
Intron
Rh6BG253200.1

Sequence Viewer

Length: 480 bp
ATGTTAGATAACGCCATTGGAAATATTATTTGGTGGTCAACAGCAACTAACATCCCTACTAATAAGAAAGAAACTGGTGATGATGATCAGTTATGTGATTTGATGAAGCTTTTACATGAATCTATCAAAAGATTGGACAAAGACTATTTGAAGACATTGCAGGGAGAAGAAGGATTTGGAGCCATTTCTGAGTTCATCGATCGCCAAATAGAGGTTGTGTATTCTTTGAAACCGGAAATATATATATTCACTAGCTGGACTAAGATTTTGAAGGAAGTTGATTTTGGATGGGGGAGACCGTTTTGGATTGGGACCATGGGGAAAGTAGGACCAACTCTTGGCAATTTCACAGTGTTCATTGAAACCCCATTAGGCATAGAAGCATGGGTGACCTTGGAGGAAAAGCAAATGGCTGTAATGGAAAATGATCCTCAGTTTTTAGCATTTACTTCTCCAAACCCACGCATTTCAAGCATGTGA

Protein Analysis

159

Amino Acids

18.15

Weight (kDa)

4.66

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 29 - 146 3.7e-11 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 338
AclWI GGATC 1 cut(s) 422
AfiI CCNNNNNNNGG 2 cut(s) 211, 338
AgsI TTSAA 5 cut(s) 151, 229, 271, 362, 471
AjuI GAANNNNNNNTTGG 6 cut(s) 13, 45, 159, 191, 267, 299
AluBI AGCT 2 cut(s) 109, 255
AluI AGCT 2 cut(s) 109, 255
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 1 cut(s) 422
ArsI GACNNNNNNTTYG 2 cut(s) 250, 282
AspS9I GGNCC 2 cut(s) 312, 329
AsuHPI GGTGA 2 cut(s) 89, 400
AvaII GGWCC 2 cut(s) 312, 329
BbsI GAAGAC 1 cut(s) 158
BccI CCATC 1 cut(s) 282
BclI TGATCA 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 1 cut(s) 252
Bme18I GGWCC 2 cut(s) 312, 329
BmgT120I GGNCC 2 cut(s) 312, 329
BmiI GGNNCC 2 cut(s) 181, 313
BpiI GAAGAC 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 2 cut(s) 315, 393
BsaWI WCCGGW 1 cut(s) 232
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 338
Bse1I ACTGG 1 cut(s) 79
Bse3DI GCAATG 1 cut(s) 155
Bse8I GATNNNNATC 1 cut(s) 84
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 2 cut(s) 315, 393
BseGI GGATG 2 cut(s) 51, 293
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 2 cut(s) 211, 338
BseMI GCAATG 1 cut(s) 155
BseMII CTCAG 2 cut(s) 180, 446
BseNI ACTGG 1 cut(s) 79
Bsh1285I CGRYCG 1 cut(s) 202
BshVI ATCGAT 1 cut(s) 198
BsiEI CGRYCG 1 cut(s) 202
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 1 cut(s) 325
BslI CCNNNNNNNGG 2 cut(s) 211, 338
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 325
Bso31I GGTCTC 1 cut(s) 289
Bsp143I GATC 3 cut(s) 85, 199, 427
Bsp19I CCATGG 1 cut(s) 315
BspCNI CTCAG 2 cut(s) 181, 445
BspDI ATCGAT 1 cut(s) 198
BspLI GGNNCC 2 cut(s) 181, 313
BspPI GGATC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 289
BsrDI GCAATG 1 cut(s) 155
BsrI ACTGG 1 cut(s) 79
BssECI CCNNGG 2 cut(s) 315, 393
BssMI GATC 3 cut(s) 85, 199, 427
BssT1I CCWWGG 2 cut(s) 315, 393
Bst4CI ACNGT 2 cut(s) 300, 352
BstDEI CTNAG 3 cut(s) 189, 261, 432
BstDSI CCRYGG 1 cut(s) 315
BstEII GGTNACC 1 cut(s) 388
BstF5I GGATG 2 cut(s) 51, 293
BstKTI GATC 3 cut(s) 88, 202, 430
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 3 cut(s) 85, 199, 427
BstMCI CGRYCG 1 cut(s) 202
BstMWI GCNNNNNNNGC 1 cut(s) 471
BstNSI RCATGY 1 cut(s) 478
BstPI GGTNACC 1 cut(s) 388
BstV2I GAAGAC 1 cut(s) 158
Bsu15I ATCGAT 1 cut(s) 198
BsuTUI ATCGAT 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 2 cut(s) 51, 293
BtsIMutI CAGTG 1 cut(s) 357
Cfr13I GGNCC 2 cut(s) 312, 329
ClaI ATCGAT 1 cut(s) 198
CviAII CATG 4 cut(s) 116, 316, 384, 475
CviJI RGCY 4 cut(s) 109, 182, 255, 413
CviKI_1 RGCY 4 cut(s) 109, 182, 255, 413
DdeI CTNAG 3 cut(s) 189, 261, 432
DpnI GATC 3 cut(s) 87, 201, 429
DpnII GATC 3 cut(s) 85, 199, 427
Eco130I CCWWGG 2 cut(s) 315, 393
Eco31I GGTCTC 1 cut(s) 289
Eco47I GGWCC 2 cut(s) 312, 329
Eco91I GGTNACC 1 cut(s) 388
EcoO65I GGTNACC 1 cut(s) 388
EcoT14I CCWWGG 2 cut(s) 315, 393
ErhI CCWWGG 2 cut(s) 315, 393
FaeI CATG 4 cut(s) 119, 319, 387, 478
FaiI YATR 9 cut(s) 94, 117, 241, 243, 245, 317, 377, 385, 476
FaqI GGGAC 1 cut(s) 325
FatI CATG 4 cut(s) 115, 315, 383, 474
FbaI TGATCA 1 cut(s) 85
FokI GGATG 2 cut(s) 38, 300
FspBI CTAG 1 cut(s) 252
HapII CCGG 1 cut(s) 233
Hin1II CATG 4 cut(s) 119, 319, 387, 478
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 1 cut(s) 119
HpaII CCGG 1 cut(s) 233
HphI GGTGA 2 cut(s) 89, 400
Hpy166II GTNNAC 1 cut(s) 39
Hpy188I TCNGA 1 cut(s) 190
Hpy8I GTNNAC 1 cut(s) 39
HpyAV CCTTC 2 cut(s) 164, 265
HpyCH4III ACNGT 2 cut(s) 300, 352
HpyCH4V TGCA 1 cut(s) 160
HpyF10VI GCNNNNNNNGC 1 cut(s) 471
HpyF3I CTNAG 3 cut(s) 189, 261, 432
Hsp92II CATG 4 cut(s) 119, 319, 387, 478
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 3 cut(s) 85, 199, 427
LmnI GCTCC 1 cut(s) 179
LpnPI CCDG 4 cut(s) 60, 146, 241, 246
MaeI CTAG 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 388
MalI GATC 3 cut(s) 87, 201, 429
MboI GATC 3 cut(s) 85, 199, 427
MboII GAAGA 2 cut(s) 163, 179
MluCI AATT 1 cut(s) 343
MnlI CCTC 3 cut(s) 205, 391, 441
MspI CCGG 1 cut(s) 233
MwoI GCNNNNNNNGC 1 cut(s) 471
NcoI CCATGG 1 cut(s) 315
NdeII GATC 3 cut(s) 85, 199, 427
NlaIII CATG 4 cut(s) 119, 319, 387, 478
NlaIV GGNNCC 2 cut(s) 181, 313
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 1 cut(s) 478
PfeI GAWTC 1 cut(s) 119
PflMI CCANNNNNTGG 1 cut(s) 338
Ple19I CGATCG 1 cut(s) 202
PspEI GGTNACC 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 181, 313
PspPI GGNCC 2 cut(s) 312, 329
PvuI CGATCG 1 cut(s) 202
Sau3AI GATC 3 cut(s) 85, 199, 427
Sau96I GGNCC 2 cut(s) 312, 329
SetI ASST 4 cut(s) 111, 216, 257, 395
SinI GGWCC 2 cut(s) 312, 329
Sse9I AATT 1 cut(s) 343
SspI AATATT 1 cut(s) 25
SspMI CTAG 1 cut(s) 252
StyI CCWWGG 2 cut(s) 315, 393
TaaI ACNGT 2 cut(s) 300, 352
TaqI TCGA 1 cut(s) 198
TasI AATT 1 cut(s) 343
TfiI GAWTC 1 cut(s) 119
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 1 cut(s) 388
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 4 cut(s) 119, 132, 184, 346
TspRI CASTG 1 cut(s) 357
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 2 cut(s) 312, 329
XceI RCATGY 1 cut(s) 478
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.