RLG00000013258

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007556
Physical Location & Seq
Reverse (-)
28768028 .. 28770432
2405 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013258

Sequence Viewer

Length: 1302 bp
ATGGAGGTGACTATTATTTCCAGAGATACCATCATTCCAGCCGCTACTTCCTCTTTAGTTCATCGCCCTAAAAAACCTTACCAACTTTCTATCTTCGATCAGCTCTCTCCCATAACTTACGTTCCATTGGTTTTCTTGTACCCCATTTTTGATATCAATGTTTCCCTAACCCTAGCCCACCTGAAAAGCTCCCTATCATATACACTCGGTGTGTACTACCCATTTTCAGGACGGACGAAAAACAACCTTTGTATTGATGATTTCGATGCGGGTGTCCCCTACCTTGAAGCCCAAATCAATTATCGCCTGTTTGAGTTTCTTAAGCTCCATGAAATCGAGCTACTCAATCAGTTTGTTCCATTCCATCCATTTCGTCAGGAAACCAAATCTCATGATGATCCACTTCCCTTATTAGCAGTTCAAGTCAGTGTTTTTTCTTGTGGTGGAATAGCAATTGGGGTCTCCTTATCTCACAAGATCCTTGATGGGGACACGGCAAACAATTTCCTTGAATCTTGGGCTGCTGTTTTTCGTGGGGATCATGACAAAATTCTGCTACACACTAATCTCTACCAAGCTTCATTAAGTTTTCCAGCAAAAGATGACATACCAGAAAAATATATGTCTTTCATGGGTGGCATTCTGTTCAGGGGGAAAAGTTATATGACAAGGAGATTTGTGTTTAATGCTAAAACCATAAACATTCTACGAGACAAAGCAAAGAGCGAGGTCGTGCCCAATCCAACACGCAGTACTTCACGAAGAGCATCAATAGCAGTGCATGCAGTGAACATAAGGCCGCGATTGAAGCCCTCGATGTTAGATAATGCCATTGGAAATATTCTTTGGTGGGCAACAACAACTAACATCCCAACTAATAAGAGAGAAATTGGTGATGATGATCAGTTGTGTGATTTGATGAAGCTTTTACATGAATCTATCAAACGATTGGACAAAGACTATTTGAAGACATTGCAGGGAGAAGAGGGATTTGGAGCCATTTCTGAGTTCATCGATCACCAAATAGAGGTTGCGTATTCTTTGAAACCAGAAACATATATATTCACCAGCTGGACTAAGATTTTCAAGGAAGTTGATTTTGGATGGGGGAGACCATTTTGGATTGGGGTCAAGGGGAAAGTAGGACCAACTCTTGGCAATCACATAGTGTTCGTTGAAACACAATGGGGCAAGGGCATAGAAGCATGGGTGACCTTGGAGGAAAAGCAAATGGCTATAATGGAAAATGATCCTCAGTTTTTAGCATTTACTTGTCCAAACCCACGCATTTCAAGCATGTGA

Protein Analysis

434

Amino Acids

49.23

Weight (kDa)

6.87

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 420 1.2e-57 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1154
AccII CGCG 1 cut(s) 802
AciI CCGC 3 cut(s) 42, 269, 800
AclWI GGATC 4 cut(s) 392, 472, 546, 1244
AcsI RAATTY 1 cut(s) 549
AdeI CACNNNGTG 2 cut(s) 209, 1168
AfaI GTAC 3 cut(s) 140, 215, 754
AfiI CCNNNNNNNGG 4 cut(s) 227, 487, 1027, 1154
AflII CTTAAG 1 cut(s) 320
AgsI TTSAA 9 cut(s) 287, 422, 512, 808, 967, 1045, 1087, 1178, 1293
AjuI GAANNNNNNNTTGG 6 cut(s) 829, 861, 975, 1007, 1083, 1115
AluBI AGCT 7 cut(s) 103, 189, 325, 340, 578, 925, 1071
AluI AGCT 7 cut(s) 103, 189, 325, 340, 578, 925, 1071
Alw26I GTCTC 3 cut(s) 466, 705, 1105
AlwI GGATC 4 cut(s) 392, 472, 546, 1244
AoxI GGCC 1 cut(s) 797
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 1 cut(s) 549
AspS9I GGNCC 1 cut(s) 1145
AsuHPI GGTGA 5 cut(s) 19, 905, 1010, 1057, 1222
AvaII GGWCC 1 cut(s) 1145
BaeGI GKGCMC 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 974
BbvI GCAGC 1 cut(s) 508
BccI CCATC 4 cut(s) 38, 372, 479, 1098
BceAI ACGGC 1 cut(s) 510
BclI TGATCA 1 cut(s) 901
BcoDI GTCTC 3 cut(s) 466, 705, 1105
BfaI CTAG 1 cut(s) 173
BfrI CTTAAG 1 cut(s) 320
BisI GCNGC 3 cut(s) 42, 522, 800
BlsI GCNGC 3 cut(s) 43, 523, 801
BmcAI AGTACT 1 cut(s) 754
Bme18I GGWCC 1 cut(s) 1145
BmgT120I GGNCC 1 cut(s) 1145
BmiI GGNNCC 1 cut(s) 997
BmsI GCATC 2 cut(s) 256, 776
BpiI GAAGAC 1 cut(s) 974
Bsa29I ATCGAT 1 cut(s) 1014
BsaBI GATNNNNATC 1 cut(s) 900
BsaI GGTCTC 2 cut(s) 466, 1105
BsaJI CCNNGG 1 cut(s) 1215
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 487, 1027, 1154
Bse3DI GCAATG 1 cut(s) 971
Bse8I GATNNNNATC 1 cut(s) 900
BseCI ATCGAT 1 cut(s) 1014
BseDI CCNNGG 1 cut(s) 1215
BseGI GGATG 3 cut(s) 364, 867, 1109
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 4 cut(s) 227, 487, 1027, 1154
BseMI GCAATG 1 cut(s) 971
BseMII CTCAG 2 cut(s) 996, 1268
BseSI GKGCMC 1 cut(s) 738
BseXI GCAGC 1 cut(s) 508
Bsh1236I CGCG 1 cut(s) 802
BshFI GGCC 1 cut(s) 799
BshVI ATCGAT 1 cut(s) 1014
BslFI GGGAC 2 cut(s) 260, 503
BslI CCNNNNNNNGG 4 cut(s) 227, 487, 1027, 1154
BsmAI GTCTC 3 cut(s) 466, 705, 1105
BsmFI GGGAC 2 cut(s) 260, 503
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 1 cut(s) 799
Bso31I GGTCTC 2 cut(s) 466, 1105
Bsp1286I GDGCHC 1 cut(s) 738
Bsp143I GATC 7 cut(s) 97, 397, 477, 538, 901, 1015, 1249
BspACI CCGC 3 cut(s) 42, 269, 800
BspANI GGCC 1 cut(s) 799
BspCNI CTCAG 2 cut(s) 997, 1267
BspDI ATCGAT 1 cut(s) 1014
BspFNI CGCG 1 cut(s) 802
BspHI TCATGA 2 cut(s) 391, 541
BspLI GGNNCC 1 cut(s) 997
BspPI GGATC 4 cut(s) 392, 472, 546, 1244
BspQI GCTCTTC 1 cut(s) 757
BspTI CTTAAG 1 cut(s) 320
BspTNI GGTCTC 2 cut(s) 466, 1105
BsrDI GCAATG 1 cut(s) 971
BssECI CCNNGG 1 cut(s) 1215
BssMI GATC 7 cut(s) 97, 397, 477, 538, 901, 1015, 1249
BssT1I CCWWGG 1 cut(s) 1215
Bst6I CTCTTC 2 cut(s) 757, 978
BstAFI CTTAAG 1 cut(s) 320
BstAPI GCANNNNNTGC 1 cut(s) 782
BstC8I GCNNGC 1 cut(s) 783
BstDEI CTNAG 3 cut(s) 1005, 1077, 1254
BstEII GGTNACC 1 cut(s) 1210
BstF5I GGATG 3 cut(s) 364, 867, 1109
BstFNI CGCG 1 cut(s) 802
BstKTI GATC 7 cut(s) 100, 400, 480, 541, 904, 1018, 1252
BstMAI GTCTC 3 cut(s) 466, 705, 1105
BstMBI GATC 7 cut(s) 97, 397, 477, 538, 901, 1015, 1249
BstMWI GCNNNNNNNGC 4 cut(s) 773, 782, 808, 1293
BstNSI RCATGY 2 cut(s) 785, 1300
BstPI GGTNACC 1 cut(s) 1210
BstSLI GKGCMC 1 cut(s) 738
BstUI CGCG 1 cut(s) 802
BstV1I GCAGC 1 cut(s) 508
BstV2I GAAGAC 1 cut(s) 974
BstX2I RGATCY 1 cut(s) 477
BstYI RGATCY 1 cut(s) 477
Bsu15I ATCGAT 1 cut(s) 1014
BsuRI GGCC 1 cut(s) 799
BsuTUI ATCGAT 1 cut(s) 1014
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 3 cut(s) 364, 867, 1109
BtsI GCAGTG 2 cut(s) 783, 792
BtsIMutI CAGTG 3 cut(s) 433, 783, 792
Cac8I GCNNGC 1 cut(s) 783
CciI TCATGA 2 cut(s) 391, 541
Cfr13I GGNCC 1 cut(s) 1145
ClaI ATCGAT 1 cut(s) 1014
Csp6I GTAC 3 cut(s) 139, 214, 753
CviAII CATG 8 cut(s) 329, 392, 542, 631, 782, 932, 1206, 1297
CviQI GTAC 3 cut(s) 139, 214, 753
DdeI CTNAG 3 cut(s) 1005, 1077, 1254
DpnI GATC 7 cut(s) 99, 399, 479, 540, 903, 1017, 1251
DpnII GATC 7 cut(s) 97, 397, 477, 538, 901, 1015, 1249
DraIII CACNNNGTG 2 cut(s) 209, 1168
Eam1104I CTCTTC 2 cut(s) 757, 978
EarI CTCTTC 2 cut(s) 757, 978
Eco130I CCWWGG 1 cut(s) 1215
Eco31I GGTCTC 2 cut(s) 466, 1105
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 1145
Eco91I GGTNACC 1 cut(s) 1210
EcoO65I GGTNACC 1 cut(s) 1210
EcoRV GATATC 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 1215
ErhI CCWWGG 1 cut(s) 1215
FaeI CATG 8 cut(s) 332, 395, 545, 634, 785, 935, 1209, 1300
FaqI GGGAC 2 cut(s) 260, 503
FatI CATG 8 cut(s) 328, 391, 541, 630, 781, 931, 1205, 1296
FauI CCCGC 1 cut(s) 262
FbaI TGATCA 1 cut(s) 901
Fnu4HI GCNGC 3 cut(s) 42, 522, 800
FokI GGATG 3 cut(s) 351, 854, 1116
Fsp4HI GCNGC 3 cut(s) 42, 522, 800
FspBI CTAG 1 cut(s) 173
GluI GCNGC 3 cut(s) 42, 522, 800
HaeIII GGCC 1 cut(s) 799
Hin1II CATG 8 cut(s) 332, 395, 545, 634, 785, 935, 1209, 1300
HindIII AAGCTT 2 cut(s) 576, 923
HinfI GANTC 2 cut(s) 512, 935
HphI GGTGA 5 cut(s) 19, 905, 1010, 1057, 1222
Hpy166II GTNNAC 2 cut(s) 214, 790
Hpy188I TCNGA 1 cut(s) 1006
Hpy188III TCNNGA 6 cut(s) 21, 228, 377, 392, 542, 759
Hpy8I GTNNAC 2 cut(s) 214, 790
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 3 cut(s) 781, 785, 976
HpyF10VI GCNNNNNNNGC 4 cut(s) 773, 782, 808, 1293
HpyF3I CTNAG 3 cut(s) 1005, 1077, 1254
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 8 cut(s) 332, 395, 545, 634, 785, 935, 1209, 1300
Ksp22I TGATCA 1 cut(s) 901
Kzo9I GATC 7 cut(s) 97, 397, 477, 538, 901, 1015, 1249
LguI GCTCTTC 1 cut(s) 757
LmnI GCTCC 3 cut(s) 194, 330, 995
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 2 cut(s) 256, 776
MaeI CTAG 1 cut(s) 173
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 2 cut(s) 7, 1210
MalI GATC 7 cut(s) 99, 399, 479, 540, 903, 1017, 1251
MboI GATC 7 cut(s) 97, 397, 477, 538, 901, 1015, 1249
MboII GAAGA 4 cut(s) 85, 774, 979, 995
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 1 cut(s) 738
MluCI AATT 5 cut(s) 298, 453, 502, 549, 888
MmeI TCCRAC 1 cut(s) 767
MnlI CCTC 7 cut(s) 61, 721, 823, 979, 1021, 1213, 1263
MseI TTAA 3 cut(s) 321, 584, 684
MspA1I CMGCKG 1 cut(s) 1071
MspCI CTTAAG 1 cut(s) 320
MunI CAATTG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 639
MvnI CGCG 1 cut(s) 802
MwoI GCNNNNNNNGC 4 cut(s) 773, 782, 808, 1293
NdeII GATC 7 cut(s) 97, 397, 477, 538, 901, 1015, 1249
NlaIII CATG 8 cut(s) 332, 395, 545, 634, 785, 935, 1209, 1300
NlaIV GGNNCC 1 cut(s) 997
NmuCI GTSAC 2 cut(s) 7, 1210
NspI RCATGY 2 cut(s) 785, 1300
PaeI GCATGC 1 cut(s) 785
PagI TCATGA 2 cut(s) 391, 541
PciSI GCTCTTC 1 cut(s) 757
PctI GAATGC 1 cut(s) 639
PfeI GAWTC 2 cut(s) 512, 935
PflMI CCANNNNNTGG 1 cut(s) 1154
PkrI GCNGC 3 cut(s) 43, 523, 801
PspEI GGTNACC 1 cut(s) 1210
PspN4I GGNNCC 1 cut(s) 997
PspPI GGNCC 1 cut(s) 1145
PsuI RGATCY 1 cut(s) 477
PvuII CAGCTG 1 cut(s) 1071
RsaI GTAC 3 cut(s) 140, 215, 754
RsaNI GTAC 3 cut(s) 139, 214, 753
SapI GCTCTTC 1 cut(s) 757
SaqAI TTAA 3 cut(s) 321, 584, 684
SatI GCNGC 3 cut(s) 42, 522, 800
Sau3AI GATC 7 cut(s) 97, 397, 477, 538, 901, 1015, 1249
Sau96I GGNCC 1 cut(s) 1145
ScaI AGTACT 1 cut(s) 754
SduI GDGCHC 1 cut(s) 738
SfaNI GCATC 2 cut(s) 256, 776
SinI GGWCC 1 cut(s) 1145
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SphI GCATGC 1 cut(s) 785
Sse9I AATT 5 cut(s) 298, 453, 502, 549, 888
SsiI CCGC 3 cut(s) 42, 269, 800
SspI AATATT 1 cut(s) 841
SspMI CTAG 1 cut(s) 173
StyI CCWWGG 1 cut(s) 1215
TaiI ACGT 1 cut(s) 123
TaqI TCGA 5 cut(s) 96, 264, 336, 815, 1014
TasI AATT 5 cut(s) 298, 453, 502, 549, 888
TatI WGTACW 2 cut(s) 213, 752
TauI GCSGC 2 cut(s) 44, 802
TfiI GAWTC 2 cut(s) 512, 935
Tru1I TTAA 3 cut(s) 321, 584, 684
Tru9I TTAA 3 cut(s) 321, 584, 684
TscAI CASTG 3 cut(s) 433, 783, 792
TseFI GTSAC 2 cut(s) 7, 1210
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 2 cut(s) 7, 1210
TspDTI ATGAA 7 cut(s) 50, 345, 570, 619, 935, 948, 1000
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 3 cut(s) 433, 783, 792
Van91I CCANNNNNTGG 1 cut(s) 1154
Vha464I CTTAAG 1 cut(s) 320
VpaK11BI GGWCC 1 cut(s) 1145
XapI RAATTY 1 cut(s) 549
XceI RCATGY 2 cut(s) 785, 1300
XspI CTAG 1 cut(s) 173
ZrmI AGTACT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.