Rw3G028630

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007556
Physical Location & Seq
Reverse (-)
43037977 .. 43040077
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G028630.1

Sequence Viewer

Length: 1371 bp
ATGAACATGGAGGTGACTATAATTTCCAGAGATAGCATCACACCAGCCGCTACTTCCTCTTCAGTTCATTGCCATAAGAAACCTTACCAACTTTCCATCATTGATCAACTCTTTCCCAAAACTTACGCTCCACTGGTTTTGTTCTACCCCATTACTAACCCAAATATCAATGTTTCCCAATCCCTAGCCCACCTGAAAAACTCCCTATCATATACACTCGTTACGTATTACCCATTTTCTGGACGGATAAAAAACAACCTTTATATTGATGATTTCGATGCGGGTGTCCCCTACCTTGAAGCCCGAATCAATTGTCGCATGTCTGAGTTTCTTAAGCTCCATGAAATTGAGCTACTCAATCAGTTCGTTCCATTCCATCTACTTCGTCAGGAAGCCCAATCTCATGATGATCTGCTGCCCTTATTAGCAATTCAAGTCAGCGTTTTTTCATGTGGTGGAATAGCAATTGGGGTCTTAGGCTCTCACAAGATCATGGATGGGGTCACGGCAAACAATTTCCTCAAATCTTGGGCTGCTGTTTTTCGTGGGGATCATGACAAAACTCTGCTACACACTAATCTCTACCAAGCTTCATTAAGTTTTCCAGCAAAAGATGACATACCAGAAAAATATCTGGCTTTCACGGATAGCATGTTTTTCAGAGGAAAACGTTATATGACAAAGAGATTTGTGTTTAATGCTAAAGCCATAGACAGTCTACGAGATAAAGCAAAGAGCGAGGTCGTGCCCAATCCAACACGTATAGAGACGCTAACTTGTTTTCTTTGGAAACACGCTACGGCTGCTTCTAGATATGGTAGCACTTCACGAAGAGCATCCATAGTAGCTCATACGGTGAACATAAGGCCGCAATTGAAGCTCTCGATGTTAGATGATGCCATTGGAAATATTTTTTGGTGGGCAATAGCGGCTAACATCCCTACTAATAAGAGAGAAAGTGGTGATGATGATCAGTTGTGTGATCTGATGAAGCTTTTACATGAATCTATAAAAGGATTGGACAATGACTATTTGAAGACATTGCAGGGAGAAGAGGGATTTGGAGCCATTTCTAAGTTCATGGATCGCTATTTAGAGGTTGCGCATTCTTGGAAACCGGAAACATATGTATTCACTAGCTGGACTAAGATTTTCAAGGAAGTTGATTTTGGATGGGGGAGACCGTTCTGGATTGGGGTCATGGGGAAAGTTAGACCAACTTTGGGCAATCTCATAGTGTTTGTTGAAACACAATGGGGTAAGGGCATAGAAGCATGGGTGACCTTGGAGGAAAAGCAAATGGCTGTAATGGAAAAAGATCCTCAATTTTTAGCATTTACTTCTCCAAACCCACGCATTTCAAGCATGTGA

Protein Analysis

456

Amino Acids

51.85

Weight (kDa)

8.4

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 3 - 443 7.7e-72 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1104
AccB7I CCANNNNNTGG 1 cut(s) 239
AccI GTMKAC 1 cut(s) 718
AciI CCGC 4 cut(s) 48, 281, 869, 929
AclI AACGTT 1 cut(s) 670
AclWI GGATC 3 cut(s) 558, 1092, 1313
AcuI CTGAAG 1 cut(s) 45
AfiI CCNNNNNNNGG 2 cut(s) 239, 1223
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 758
AgsI TTSAA 7 cut(s) 299, 434, 877, 1036, 1156, 1247, 1362
AjuI GAANNNNNNNTTGG 6 cut(s) 898, 930, 1044, 1076, 1152, 1184
AluBI AGCT 7 cut(s) 337, 352, 590, 848, 880, 994, 1140
AluI AGCT 7 cut(s) 337, 352, 590, 848, 880, 994, 1140
Alw26I GTCTC 2 cut(s) 761, 1174
AlwI GGATC 3 cut(s) 558, 1092, 1313
AoxI GGCC 1 cut(s) 866
ApeKI GCWGC 3 cut(s) 415, 533, 803
ArsI GACNNNNNNTTYG 2 cut(s) 298, 330
AspLEI GCGC 1 cut(s) 1105
AsuHPI GGTGA 4 cut(s) 25, 868, 974, 1291
BaeGI GKGCMC 1 cut(s) 750
BbsI GAAGAC 1 cut(s) 1043
BbvI GCAGC 3 cut(s) 402, 520, 790
BccI CCATC 4 cut(s) 104, 384, 491, 1167
BceAI ACGGC 2 cut(s) 522, 816
BclI TGATCA 2 cut(s) 103, 970
BcoDI GTCTC 2 cut(s) 761, 1174
BfaI CTAG 3 cut(s) 185, 810, 1137
BfrI CTTAAG 1 cut(s) 332
BisI GCNGC 6 cut(s) 48, 416, 534, 804, 869, 930
BlsI GCNGC 6 cut(s) 49, 417, 535, 805, 870, 931
BmiI GGNNCC 1 cut(s) 1066
BmsI GCATC 4 cut(s) 45, 268, 845, 886
BpiI GAAGAC 1 cut(s) 1043
BsaAI YACGTR 2 cut(s) 225, 761
BsaBI GATNNNNATC 1 cut(s) 969
BsaI GGTCTC 1 cut(s) 1174
BsaJI CCNNGG 1 cut(s) 1284
BsaWI WCCGGW 1 cut(s) 1117
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 1223
Bse1I ACTGG 1 cut(s) 138
Bse3DI GCAATG 2 cut(s) 67, 1040
Bse8I GATNNNNATC 1 cut(s) 969
BseDI CCNNGG 1 cut(s) 1284
BseGI GGATG 4 cut(s) 502, 836, 936, 1178
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 2 cut(s) 239, 1223
BseMI GCAATG 2 cut(s) 67, 1040
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 1 cut(s) 138
BseSI GKGCMC 1 cut(s) 750
BseXI GCAGC 3 cut(s) 402, 520, 790
BshFI GGCC 1 cut(s) 868
BsiSI CCGG 1 cut(s) 1118
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 2 cut(s) 239, 1223
BsmAI GTCTC 2 cut(s) 761, 1174
BsmBI CGTCTC 1 cut(s) 761
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 1105
BsnI GGCC 1 cut(s) 868
Bso31I GGTCTC 1 cut(s) 1174
Bsp1286I GDGCHC 1 cut(s) 750
Bsp143I GATC 8 cut(s) 103, 409, 489, 550, 970, 982, 1084, 1318
BspACI CCGC 4 cut(s) 48, 281, 869, 929
BspANI GGCC 1 cut(s) 868
BspCNI CTCAG 1 cut(s) 316
BspHI TCATGA 2 cut(s) 403, 553
BspLI GGNNCC 1 cut(s) 1066
BspPI GGATC 3 cut(s) 558, 1092, 1313
BspQI GCTCTTC 1 cut(s) 826
BspTI CTTAAG 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 1174
BsrDI GCAATG 2 cut(s) 67, 1040
BsrI ACTGG 1 cut(s) 138
BssECI CCNNGG 1 cut(s) 1284
BssMI GATC 8 cut(s) 103, 409, 489, 550, 970, 982, 1084, 1318
BssT1I CCWWGG 1 cut(s) 1284
Bst4CI ACNGT 3 cut(s) 716, 856, 1185
Bst6I CTCTTC 3 cut(s) 64, 826, 1047
BstAFI CTTAAG 1 cut(s) 332
BstBAI YACGTR 2 cut(s) 225, 761
BstDEI CTNAG 4 cut(s) 324, 475, 1074, 1146
BstEII GGTNACC 1 cut(s) 1279
BstF5I GGATG 4 cut(s) 502, 836, 936, 1178
BstHHI GCGC 1 cut(s) 1105
BstKTI GATC 8 cut(s) 106, 412, 492, 553, 973, 985, 1087, 1321
BstMAI GTCTC 2 cut(s) 761, 1174
BstMBI GATC 8 cut(s) 103, 409, 489, 550, 970, 982, 1084, 1318
BstMWI GCNNNNNNNGC 4 cut(s) 803, 877, 929, 1362
BstNSI RCATGY 3 cut(s) 322, 655, 1369
BstPI GGTNACC 1 cut(s) 1279
BstSLI GKGCMC 1 cut(s) 750
BstSNI TACGTA 1 cut(s) 225
BstV1I GCAGC 3 cut(s) 402, 520, 790
BstV2I GAAGAC 1 cut(s) 1043
BstX2I RGATCY 1 cut(s) 1318
BstYI RGATCY 1 cut(s) 1318
BsuRI GGCC 1 cut(s) 868
BtsCI GGATG 4 cut(s) 502, 836, 936, 1178
BtsIMutI CAGTG 1 cut(s) 131
CciI TCATGA 2 cut(s) 403, 553
CfoI GCGC 1 cut(s) 1105
CseI GACGC 1 cut(s) 778
CspCI CAANNNNNGTGG 2 cut(s) 120, 155
DdeI CTNAG 4 cut(s) 324, 475, 1074, 1146
DpnI GATC 8 cut(s) 105, 411, 491, 552, 972, 984, 1086, 1320
DpnII GATC 8 cut(s) 103, 409, 489, 550, 970, 982, 1084, 1318
Eam1104I CTCTTC 3 cut(s) 64, 826, 1047
EarI CTCTTC 3 cut(s) 64, 826, 1047
Eco105I TACGTA 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 1284
Eco31I GGTCTC 1 cut(s) 1174
Eco57I CTGAAG 1 cut(s) 45
Eco91I GGTNACC 1 cut(s) 1279
EcoO65I GGTNACC 1 cut(s) 1279
EcoT14I CCWWGG 1 cut(s) 1284
ErhI CCWWGG 1 cut(s) 1284
Esp3I CGTCTC 1 cut(s) 761
FaqI GGGAC 1 cut(s) 272
FauI CCCGC 1 cut(s) 274
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 2 cut(s) 103, 970
FblI GTMKAC 1 cut(s) 718
Fnu4HI GCNGC 6 cut(s) 48, 416, 534, 804, 869, 930
FokI GGATG 4 cut(s) 509, 823, 923, 1185
Fsp4HI GCNGC 6 cut(s) 48, 416, 534, 804, 869, 930
FspBI CTAG 3 cut(s) 185, 810, 1137
FspI TGCGCA 1 cut(s) 1104
GlaI GCGC 1 cut(s) 1104
GluI GCNGC 6 cut(s) 48, 416, 534, 804, 869, 930
HaeIII GGCC 1 cut(s) 868
HapII CCGG 1 cut(s) 1118
HgaI GACGC 1 cut(s) 778
HhaI GCGC 1 cut(s) 1105
Hin6I GCGC 1 cut(s) 1103
HinP1I GCGC 1 cut(s) 1103
HindIII AAGCTT 2 cut(s) 588, 992
HinfI GANTC 2 cut(s) 306, 1004
HpaII CCGG 1 cut(s) 1118
HphI GGTGA 4 cut(s) 25, 868, 974, 1291
Hpy166II GTNNAC 2 cut(s) 719, 859
Hpy188I TCNGA 3 cut(s) 325, 662, 987
Hpy188III TCNNGA 9 cut(s) 27, 240, 389, 404, 554, 810, 828, 883, 1189
Hpy8I GTNNAC 2 cut(s) 719, 859
HpyCH4III ACNGT 3 cut(s) 716, 856, 1185
HpyCH4IV ACGT 3 cut(s) 224, 670, 760
HpyCH4V TGCA 1 cut(s) 1045
HpyF10VI GCNNNNNNNGC 4 cut(s) 803, 877, 929, 1362
HpyF3I CTNAG 4 cut(s) 324, 475, 1074, 1146
HpySE526I ACGT 3 cut(s) 224, 670, 760
HspAI GCGC 1 cut(s) 1103
Ksp22I TGATCA 2 cut(s) 103, 970
Kzo9I GATC 8 cut(s) 103, 409, 489, 550, 970, 982, 1084, 1318
LguI GCTCTTC 1 cut(s) 826
LmnI GCTCC 3 cut(s) 133, 342, 1064
Lsp1109I GCAGC 3 cut(s) 402, 520, 790
LweI GCATC 4 cut(s) 45, 268, 845, 886
MaeI CTAG 3 cut(s) 185, 810, 1137
MaeII ACGT 3 cut(s) 224, 670, 760
MaeIII GTNAC 4 cut(s) 13, 220, 502, 1279
MalI GATC 8 cut(s) 105, 411, 491, 552, 972, 984, 1086, 1320
MboI GATC 8 cut(s) 103, 409, 489, 550, 970, 982, 1084, 1318
MboII GAAGA 4 cut(s) 51, 843, 1048, 1064
MfeI CAATTG 3 cut(s) 310, 465, 872
MflI RGATCY 1 cut(s) 1318
MhlI GDGCHC 1 cut(s) 750
MluCI AATT 8 cut(s) 21, 310, 345, 429, 465, 514, 872, 1325
MmeI TCCRAC 1 cut(s) 779
MnlI CCTC 9 cut(s) 4, 67, 530, 656, 733, 1048, 1090, 1282, 1332
MseI TTAA 3 cut(s) 333, 596, 696
MslI CAYNNNNRTG 1 cut(s) 11
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 1 cut(s) 1118
MunI CAATTG 3 cut(s) 310, 465, 872
Mva1269I GAATGC 1 cut(s) 1105
MwoI GCNNNNNNNGC 4 cut(s) 803, 877, 929, 1362
NdeI CATATG 1 cut(s) 1126
NdeII GATC 8 cut(s) 103, 409, 489, 550, 970, 982, 1084, 1318
NlaIV GGNNCC 1 cut(s) 1066
NmuCI GTSAC 3 cut(s) 13, 502, 1279
NsbI TGCGCA 1 cut(s) 1104
NspI RCATGY 3 cut(s) 322, 655, 1369
PagI TCATGA 2 cut(s) 403, 553
PciSI GCTCTTC 1 cut(s) 826
PctI GAATGC 1 cut(s) 1105
PfeI GAWTC 2 cut(s) 306, 1004
PflMI CCANNNNNTGG 1 cut(s) 239
PkrI GCNGC 6 cut(s) 49, 417, 535, 805, 870, 931
Ppu21I YACGTR 2 cut(s) 225, 761
Psp1406I AACGTT 1 cut(s) 670
PspEI GGTNACC 1 cut(s) 1279
PspN4I GGNNCC 1 cut(s) 1066
PsuI RGATCY 1 cut(s) 1318
RseI CAYNNNNRTG 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 826
SaqAI TTAA 3 cut(s) 333, 596, 696
SatI GCNGC 6 cut(s) 48, 416, 534, 804, 869, 930
Sau3AI GATC 8 cut(s) 103, 409, 489, 550, 970, 982, 1084, 1318
SduI GDGCHC 1 cut(s) 750
SfaNI GCATC 4 cut(s) 45, 268, 845, 886
SmiMI CAYNNNNRTG 1 cut(s) 11
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SnaBI TACGTA 1 cut(s) 225
Sse9I AATT 8 cut(s) 21, 310, 345, 429, 465, 514, 872, 1325
SsiI CCGC 4 cut(s) 48, 281, 869, 929
SspI AATATT 1 cut(s) 910
SspMI CTAG 3 cut(s) 185, 810, 1137
StyI CCWWGG 1 cut(s) 1284
TaaI ACNGT 3 cut(s) 716, 856, 1185
TaiI ACGT 3 cut(s) 227, 673, 763
TaqI TCGA 2 cut(s) 276, 884
TasI AATT 8 cut(s) 21, 310, 345, 429, 465, 514, 872, 1325
TauI GCSGC 3 cut(s) 50, 871, 932
TfiI GAWTC 2 cut(s) 306, 1004
Tru1I TTAA 3 cut(s) 333, 596, 696
Tru9I TTAA 3 cut(s) 333, 596, 696
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 3 cut(s) 13, 502, 1279
TseI GCWGC 3 cut(s) 415, 533, 803
Tsp45I GTSAC 3 cut(s) 13, 502, 1279
TspDTI ATGAA 8 cut(s) 17, 56, 357, 438, 582, 1004, 1017, 1069
TspGWI ACGGA 2 cut(s) 259, 659
TspRI CASTG 1 cut(s) 138
Van91I CCANNNNNTGG 1 cut(s) 239
Vha464I CTTAAG 1 cut(s) 332
XbaI TCTAGA 1 cut(s) 809
XceI RCATGY 3 cut(s) 322, 655, 1369
XmiI GTMKAC 1 cut(s) 718
XspI CTAG 3 cut(s) 185, 810, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.