Rh1AG306900

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0007556
Physical Location & Seq
Forward (+)
54051312 .. 54078025
26714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG306900.1

Sequence Viewer

Length: 678 bp
ATGTCACCTGTGAAGACATCCATAGTCGTTCATGCAGTGAACATGAGACCGCGAATGAATTCACAAATGCGATCGGATACTGTCATGATCGGAAATCTTTTTTGGTGGGCCGTAGCTGCCGCCGACGCTTCTGAGGCAGACGCTCATGACTTGCGTGACTTAGTGAACATAATTAAGGAATCAATCAAGGGATTCGATGGTGAGTATTTGGAGAGTTTGCAAGGTGAAGATGGTGGATTCGGAACCATTTCTGAGTTTTGTATTCAGCTTGAGGCTATGCTTTCCTCAGAAACACCGGATATCTATGCATTCACAAGCTGGGCTAGCGTTTTAAAACAGGTTGATTTTGGTTGGGGGAGGCCATTTTGGATCGGAGCCATGGGGAAAGTTGGTCCTGCATTTAGAAATCTCACTGTGTTCGTCGACAGACAATGGGATAGGGGCATTGAAGCATGGGTGAACTTGGAGGAAAACCAAATGGCTCTGTTAGAGAAAGACCCCCAGTTTTTAGCATTTGCTTCTCCAAATCCTGGAATATCAAGCATGTACTTCACCAAAGACATCCATACTCGTTCATGCAGTGAACATGAGACCGCGAATGAATTCACAAATGCAATCGGATACTGTCATGATCGGAAATCTTTTTTGGTGGGCCGTAGCTGCCGCCGATGCTTCTGA

Protein Analysis

225

Amino Acids

25.35

Weight (kDa)

5.14

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 3 - 169 1.5e-18 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 530
AccI GTMKAC 1 cut(s) 423
AccII CGCG 2 cut(s) 52, 596
AciI CCGC 4 cut(s) 50, 120, 594, 664
AclWI GGATC 1 cut(s) 377
AcsI RAATTY 2 cut(s) 58, 602
AfaI GTAC 1 cut(s) 548
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 1 cut(s) 449
AjnI CCWGG 1 cut(s) 529
AjuI GAANNNNNNNTTGG 4 cut(s) 85, 117, 629, 661
AluBI AGCT 4 cut(s) 116, 268, 318, 660
AluI AGCT 4 cut(s) 116, 268, 318, 660
Alw26I GTCTC 2 cut(s) 40, 584
AlwI GGATC 1 cut(s) 377
AoxI GGCC 3 cut(s) 108, 359, 652
ApeKI GCWGC 2 cut(s) 116, 660
ApoI RAATTY 2 cut(s) 58, 602
Asp700I GAANNNNTTC 3 cut(s) 58, 247, 602
AspS9I GGNCC 3 cut(s) 108, 392, 652
AsuHPI GGTGA 4 cut(s) 212, 236, 469, 544
AsuNHI GCTAGC 1 cut(s) 323
AvaII GGWCC 1 cut(s) 392
BbsI GAAGAC 1 cut(s) 20
BbvI GCAGC 2 cut(s) 103, 647
BccI CCATC 2 cut(s) 191, 224
BceAI ACGGC 2 cut(s) 95, 639
BciT130I CCWGG 1 cut(s) 531
BciVI GTATCC 2 cut(s) 70, 614
BcoDI GTCTC 2 cut(s) 40, 584
BfaI CTAG 1 cut(s) 324
BfuI GTATCC 2 cut(s) 70, 614
BisI GCNGC 4 cut(s) 117, 120, 661, 664
BlsI GCNGC 4 cut(s) 118, 121, 662, 665
Bme1390I CCNGG 1 cut(s) 531
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 3 cut(s) 108, 392, 652
BmiI GGNNCC 2 cut(s) 244, 376
BmrFI CCNGG 1 cut(s) 531
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 1 cut(s) 659
BmtI GCTAGC 1 cut(s) 327
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 290
BsaI GGTCTC 2 cut(s) 40, 584
BsaJI CCNNGG 1 cut(s) 378
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 502
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 1 cut(s) 378
BseGI GGATG 2 cut(s) 17, 561
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMII CTCAG 3 cut(s) 123, 243, 300
BseNI ACTGG 1 cut(s) 502
BseXI GCAGC 2 cut(s) 103, 647
BseYI CCCAGC 1 cut(s) 318
Bsh1236I CGCG 2 cut(s) 52, 596
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 3 cut(s) 110, 361, 654
BsiEI CGRYCG 1 cut(s) 74
BsiSI CCGG 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 2 cut(s) 40, 584
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 3 cut(s) 110, 361, 654
Bso31I GGTCTC 2 cut(s) 40, 584
Bsp143I GATC 4 cut(s) 71, 87, 369, 631
Bsp19I CCATGG 1 cut(s) 378
BspACI CCGC 4 cut(s) 50, 120, 594, 664
BspANI GGCC 3 cut(s) 110, 361, 654
BspCNI CTCAG 3 cut(s) 124, 244, 299
BspFNI CGCG 2 cut(s) 52, 596
BspHI TCATGA 3 cut(s) 84, 145, 628
BspLI GGNNCC 2 cut(s) 244, 376
BspOI GCTAGC 1 cut(s) 327
BspPI GGATC 1 cut(s) 377
BspTNI GGTCTC 2 cut(s) 40, 584
BsrI ACTGG 1 cut(s) 502
BssECI CCNNGG 1 cut(s) 378
BssMI GATC 4 cut(s) 71, 87, 369, 631
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 3 cut(s) 82, 415, 626
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 4 cut(s) 132, 160, 252, 286
BstDSI CCRYGG 1 cut(s) 378
BstF5I GGATG 2 cut(s) 17, 561
BstFNI CGCG 2 cut(s) 52, 596
BstKTI GATC 4 cut(s) 74, 90, 372, 634
BstMAI GTCTC 2 cut(s) 40, 584
BstMBI GATC 4 cut(s) 71, 87, 369, 631
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 6 cut(s) 116, 125, 134, 324, 660, 669
BstNI CCWGG 1 cut(s) 531
BstNSI RCATGY 1 cut(s) 547
BstSCI CCNGG 1 cut(s) 529
BstUI CGCG 2 cut(s) 52, 596
BstV1I GCAGC 2 cut(s) 103, 647
BstV2I GAAGAC 1 cut(s) 20
BsuI GTATCC 2 cut(s) 70, 614
BsuRI GGCC 3 cut(s) 110, 361, 654
BtgI CCRYGG 1 cut(s) 378
BtsCI GGATG 2 cut(s) 17, 561
BtsI GCAGTG 2 cut(s) 42, 586
BtsIMutI CAGTG 3 cut(s) 42, 411, 586
Cac8I GCNNGC 1 cut(s) 325
CciI TCATGA 3 cut(s) 84, 145, 628
Cfr13I GGNCC 3 cut(s) 108, 392, 652
CseI GACGC 2 cut(s) 134, 149
Csp6I GTAC 1 cut(s) 547
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 4 cut(s) 132, 160, 252, 286
DpnI GATC 4 cut(s) 73, 89, 371, 633
DpnII GATC 4 cut(s) 71, 87, 369, 631
DraI TTTAAA 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 378
Eco31I GGTCTC 2 cut(s) 40, 584
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 392
EcoRI GAATTC 2 cut(s) 58, 602
EcoRII CCWGG 1 cut(s) 529
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 378
EcoT22I ATGCAT 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 378
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 4 cut(s) 117, 120, 661, 664
FokI GGATG 2 cut(s) 4, 548
Fsp4HI GCNGC 4 cut(s) 117, 120, 661, 664
FspBI CTAG 1 cut(s) 324
GluI GCNGC 4 cut(s) 117, 120, 661, 664
GsaI CCCAGC 1 cut(s) 322
HaeIII GGCC 3 cut(s) 110, 361, 654
HapII CCGG 1 cut(s) 296
HgaI GACGC 2 cut(s) 134, 149
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 3 cut(s) 179, 192, 237
HpaII CCGG 1 cut(s) 296
HphI GGTGA 4 cut(s) 212, 236, 469, 544
Hpy166II GTNNAC 5 cut(s) 40, 166, 424, 460, 584
Hpy188III TCNNGA 3 cut(s) 85, 146, 629
Hpy8I GTNNAC 5 cut(s) 40, 166, 424, 460, 584
Hpy99I CGWCG 2 cut(s) 128, 425
HpyCH4III ACNGT 3 cut(s) 82, 415, 626
HpyCH4V TGCA 6 cut(s) 35, 220, 308, 398, 579, 614
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 125, 134, 324, 660, 669
HpyF3I CTNAG 4 cut(s) 132, 160, 252, 286
Kzo9I GATC 4 cut(s) 71, 87, 369, 631
LmnI GCTCC 1 cut(s) 374
LpnPI CCDG 8 cut(s) 21, 304, 309, 323, 408, 515, 516, 543
Lsp1109I GCAGC 2 cut(s) 103, 647
LweI GCATC 1 cut(s) 659
MaeI CTAG 1 cut(s) 324
MaeIII GTNAC 2 cut(s) 3, 155
MalI GATC 4 cut(s) 73, 89, 371, 633
MboI GATC 4 cut(s) 71, 87, 369, 631
MboII GAAGA 2 cut(s) 25, 239
MluCI AATT 3 cut(s) 58, 171, 602
MnlI CCTC 5 cut(s) 127, 265, 295, 351, 460
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 3 cut(s) 58, 247, 602
MseI TTAA 2 cut(s) 174, 332
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 531
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 1 cut(s) 531
MvnI CGCG 2 cut(s) 52, 596
MwoI GCNNNNNNNGC 6 cut(s) 116, 125, 134, 324, 660, 669
NcoI CCATGG 1 cut(s) 378
NdeII GATC 4 cut(s) 71, 87, 369, 631
NheI GCTAGC 1 cut(s) 323
NlaIV GGNNCC 2 cut(s) 244, 376
NmuCI GTSAC 2 cut(s) 3, 155
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 1 cut(s) 547
PagI TCATGA 3 cut(s) 84, 145, 628
PctI GAATGC 1 cut(s) 308
PdmI GAANNNNTTC 3 cut(s) 58, 247, 602
PfeI GAWTC 3 cut(s) 179, 192, 237
PflMI CCANNNNNTGG 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 529
PkrI GCNGC 4 cut(s) 118, 121, 662, 665
Ple19I CGATCG 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 529
PspFI CCCAGC 1 cut(s) 318
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 2 cut(s) 244, 376
PspPI GGNCC 3 cut(s) 108, 392, 652
PvuI CGATCG 1 cut(s) 74
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
SalI GTCGAC 1 cut(s) 422
SaqAI TTAA 2 cut(s) 174, 332
SatI GCNGC 4 cut(s) 117, 120, 661, 664
Sau3AI GATC 4 cut(s) 71, 87, 369, 631
Sau96I GGNCC 3 cut(s) 108, 392, 652
ScrFI CCNGG 1 cut(s) 531
SetI ASST 7 cut(s) 10, 118, 226, 270, 320, 342, 662
SfaNI GCATC 1 cut(s) 659
SinI GGWCC 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 3 cut(s) 58, 171, 602
SsiI CCGC 4 cut(s) 50, 120, 594, 664
SspMI CTAG 1 cut(s) 324
StyD4I CCNGG 1 cut(s) 529
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 3 cut(s) 82, 415, 626
TaqI TCGA 2 cut(s) 195, 423
TasI AATT 3 cut(s) 58, 171, 602
TatI WGTACW 1 cut(s) 546
TauI GCSGC 2 cut(s) 122, 666
TfiI GAWTC 3 cut(s) 179, 192, 237
Tru1I TTAA 2 cut(s) 174, 332
Tru9I TTAA 2 cut(s) 174, 332
TscAI CASTG 3 cut(s) 42, 418, 586
TseFI GTSAC 2 cut(s) 3, 155
TseI GCWGC 2 cut(s) 116, 660
Tsp45I GTSAC 2 cut(s) 3, 155
TspDTI ATGAA 4 cut(s) 20, 71, 564, 615
TspRI CASTG 3 cut(s) 42, 418, 586
Van91I CCANNNNNTGG 1 cut(s) 530
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 2 cut(s) 58, 602
XceI RCATGY 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 3 cut(s) 58, 247, 602
XspI CTAG 1 cut(s) 324
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.