Rroxscaffold_7G00190810

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007556
Physical Location & Seq
Forward (+)
31148967 .. 31151380
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190810.1

Sequence Viewer

Length: 1089 bp
ATGCAGGTGACTATTATTTCCCGAGATACCATCATACCAGCCGCTACTTCCTCTTCAGTTCTTCACCATAAGAAACCTTACCAACTTTGTATCTTTGATCAACTCTTTCCCATAACTTACGTTCCATTGGTTTTCTTCTACCCCATTTTTGACCCAAATATCAATGTTTCCCAAGCCCTAGCCCACCTGAAACGCTCCCTATCATATACACTCGGTGTGTACTACCCATTTTCGGGACGGACGAAAAACAACCTTTGTATTGATGATTTCGATGCGGGTGTCCCCTACCTTGAAGCCCAAATCAATTATCGCCTGTTTGAGTTTCTTAAGGTCCATGAAATCGAGCTACTCAATCAGTTCGTTCCATTCCATCCACTTCGCCAAGAAACCGAATCTCATGATGATCCGCTGCCCTTATTAGCAGTTCAAGTCGGTGTTTTTTCTTGTGGTGGAATAGCAATTGGGGTCTCCATGTCTCACAAGATCTTTGATGGGGACACGGCAAACAATTTCCTTAAATCTTGGGCTGCTGTTTTTCGTGGGGATCATGACAAAATTCCGCTGCACACTAATCTCTACCAAGCTTCATTAAGTTTTCCAGCAAAAGATGACATACCAGAAAAATATGTGTCTTTCATGGGTAGCTTTCTGTTCGGAGGGAAAAGTTATATCTCAAGGAGATTTGTGTTTAATGCTAAAGCCATAGACATTCTACGAGCCAAAGCAAAGAGCGAGGTCGTGCCCAATCCAACACGTATAGAGACACTAACTTGTTTTCTTTGGAAACACGCTACAGCTGCTTCTAGATATGGTAGTGCTTCACCAAGAGCATCAGTAGCAGTGCATGAAGTGAACATAAGGTCGCGATTGAAGCCCTTGATGTTAGATAACGCCATTGGAAATATTTGTTGGTGGGCAACATCAACTAACATCCCTACTAATAAAAGAGAAACTGCTGATGATGATCAGTTGTGTGATTTGATGAAGCTTTTACATGAATCTATCAAAAGATTGGACAAAGACTATTTGAAGACATTGCAGGGAGAAAAGGGATTTAGAGCCATTTCGAGTTCATTGATCGCCAAATAG

Protein Analysis

362

Amino Acids

40.84

Weight (kDa)

8.74

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 344 1.5e-56 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 865
AciI CCGC 4 cut(s) 42, 275, 407, 560
AclWI GGATC 2 cut(s) 398, 552
AcsI RAATTY 1 cut(s) 555
AcuI CTGAAG 1 cut(s) 39
AdeI CACNNNGTG 1 cut(s) 215
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 2 cut(s) 232, 233
AflII CTTAAG 1 cut(s) 326
AflIII ACRYGT 1 cut(s) 752
AgsI TTSAA 4 cut(s) 293, 428, 871, 1030
AjuI GAANNNNNNNTTGG 2 cut(s) 892, 924
AluBI AGCT 5 cut(s) 346, 584, 645, 797, 988
AluI AGCT 5 cut(s) 346, 584, 645, 797, 988
Alw26I GTCTC 3 cut(s) 472, 480, 755
AlwI GGATC 2 cut(s) 398, 552
Ama87I CYCGRG 1 cut(s) 21
ApeKI GCWGC 4 cut(s) 409, 527, 562, 797
ApoI RAATTY 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 331
AsuHPI GGTGA 3 cut(s) 19, 56, 813
AvaI CYCGRG 1 cut(s) 21
AvaII GGWCC 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 744
BbsI GAAGAC 1 cut(s) 1037
BbvI GCAGC 4 cut(s) 396, 514, 549, 784
BccI CCATC 3 cut(s) 38, 378, 485
BceAI ACGGC 1 cut(s) 516
BclI TGATCA 2 cut(s) 97, 964
BcoDI GTCTC 3 cut(s) 472, 480, 755
BfaI CTAG 2 cut(s) 179, 804
BfmI CTRYAG 1 cut(s) 792
BfrI CTTAAG 1 cut(s) 326
BglII AGATCT 1 cut(s) 483
BisI GCNGC 5 cut(s) 42, 410, 528, 563, 798
BlsI GCNGC 5 cut(s) 43, 411, 529, 564, 799
Bme18I GGWCC 1 cut(s) 331
BmeT110I CYCGRG 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 331
BmsI GCATC 2 cut(s) 262, 839
BpiI GAAGAC 1 cut(s) 1037
BpuEI CTTGAG 1 cut(s) 658
BsaAI YACGTR 1 cut(s) 755
BsaBI GATNNNNATC 1 cut(s) 963
BsaI GGTCTC 1 cut(s) 472
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 233
Bse3DI GCAATG 1 cut(s) 1034
Bse8I GATNNNNATC 1 cut(s) 963
BseGI GGATG 2 cut(s) 370, 930
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 2 cut(s) 232, 233
BseMI GCAATG 1 cut(s) 1034
BseSI GKGCMC 1 cut(s) 744
BseXI GCAGC 4 cut(s) 396, 514, 549, 784
BsgI GTGCAG 1 cut(s) 548
Bsh1236I CGCG 1 cut(s) 865
BsiHKCI CYCGRG 1 cut(s) 21
BslFI GGGAC 3 cut(s) 249, 266, 509
BslI CCNNNNNNNGG 2 cut(s) 232, 233
BsmAI GTCTC 3 cut(s) 472, 480, 755
BsmFI GGGAC 3 cut(s) 249, 266, 509
Bso31I GGTCTC 1 cut(s) 472
BsoBI CYCGRG 1 cut(s) 21
Bsp1286I GDGCHC 1 cut(s) 744
Bsp143I GATC 6 cut(s) 97, 403, 483, 544, 964, 1077
Bsp68I TCGCGA 1 cut(s) 865
BspACI CCGC 4 cut(s) 42, 275, 407, 560
BspFNI CGCG 1 cut(s) 865
BspHI TCATGA 2 cut(s) 397, 547
BspPI GGATC 2 cut(s) 398, 552
BspTI CTTAAG 1 cut(s) 326
BspTNI GGTCTC 1 cut(s) 472
BsrDI GCAATG 1 cut(s) 1034
BssMI GATC 6 cut(s) 97, 403, 483, 544, 964, 1077
Bst6I CTCTTC 1 cut(s) 58
BstAFI CTTAAG 1 cut(s) 326
BstBAI YACGTR 1 cut(s) 755
BstF5I GGATG 2 cut(s) 370, 930
BstFNI CGCG 1 cut(s) 865
BstKTI GATC 6 cut(s) 100, 406, 486, 547, 967, 1080
BstMAI GTCTC 3 cut(s) 472, 480, 755
BstMBI GATC 6 cut(s) 97, 403, 483, 544, 964, 1077
BstMWI GCNNNNNNNGC 3 cut(s) 797, 836, 871
BstSFI CTRYAG 1 cut(s) 792
BstSLI GKGCMC 1 cut(s) 744
BstUI CGCG 1 cut(s) 865
BstV1I GCAGC 4 cut(s) 396, 514, 549, 784
BstV2I GAAGAC 1 cut(s) 1037
BstX2I RGATCY 1 cut(s) 483
BstYI RGATCY 1 cut(s) 483
BtsCI GGATG 2 cut(s) 370, 930
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 1 cut(s) 846
BtuMI TCGCGA 1 cut(s) 865
CciI TCATGA 2 cut(s) 397, 547
Cfr13I GGNCC 1 cut(s) 331
Csp6I GTAC 1 cut(s) 220
CviAII CATG 7 cut(s) 335, 398, 472, 548, 637, 845, 995
CviQI GTAC 1 cut(s) 220
DpnI GATC 6 cut(s) 99, 405, 485, 546, 966, 1079
DpnII GATC 6 cut(s) 97, 403, 483, 544, 964, 1077
DraIII CACNNNGTG 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 58
Eco31I GGTCTC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 21
FaeI CATG 7 cut(s) 338, 401, 475, 551, 640, 848, 998
FaqI GGGAC 3 cut(s) 249, 266, 509
FatI CATG 7 cut(s) 334, 397, 471, 547, 636, 844, 994
FauI CCCGC 1 cut(s) 268
FbaI TGATCA 2 cut(s) 97, 964
Fnu4HI GCNGC 5 cut(s) 42, 410, 528, 563, 798
FokI GGATG 2 cut(s) 357, 917
Fsp4HI GCNGC 5 cut(s) 42, 410, 528, 563, 798
FspBI CTAG 2 cut(s) 179, 804
GluI GCNGC 5 cut(s) 42, 410, 528, 563, 798
Hin1II CATG 7 cut(s) 338, 401, 475, 551, 640, 848, 998
HindIII AAGCTT 2 cut(s) 582, 986
HinfI GANTC 2 cut(s) 392, 998
HphI GGTGA 3 cut(s) 19, 56, 813
Hpy166II GTNNAC 2 cut(s) 220, 853
Hpy188I TCNGA 1 cut(s) 656
Hpy188III TCNNGA 6 cut(s) 21, 234, 398, 548, 804, 864
Hpy8I GTNNAC 2 cut(s) 220, 853
HpyCH4IV ACGT 2 cut(s) 120, 754
HpyCH4V TGCA 4 cut(s) 4, 565, 844, 1039
HpyF10VI GCNNNNNNNGC 3 cut(s) 797, 836, 871
HpySE526I ACGT 2 cut(s) 120, 754
Hsp92II CATG 7 cut(s) 338, 401, 475, 551, 640, 848, 998
Ksp22I TGATCA 2 cut(s) 97, 964
Kzo9I GATC 6 cut(s) 97, 403, 483, 544, 964, 1077
LmnI GCTCC 1 cut(s) 200
LpnPI CCDG 6 cut(s) 51, 200, 326, 612, 630, 1025
Lsp1109I GCAGC 4 cut(s) 396, 514, 549, 784
LweI GCATC 2 cut(s) 262, 839
MaeI CTAG 2 cut(s) 179, 804
MaeII ACGT 2 cut(s) 120, 754
MaeIII GTNAC 1 cut(s) 7
MalI GATC 6 cut(s) 99, 405, 485, 546, 966, 1079
MboI GATC 6 cut(s) 97, 403, 483, 544, 964, 1077
MboII GAAGA 4 cut(s) 45, 53, 127, 1042
MfeI CAATTG 1 cut(s) 459
MflI RGATCY 1 cut(s) 483
MhlI GDGCHC 1 cut(s) 744
MluCI AATT 4 cut(s) 304, 459, 508, 555
MmeI TCCRAC 1 cut(s) 773
MnlI CCTC 3 cut(s) 61, 650, 727
MseI TTAA 4 cut(s) 327, 516, 590, 690
MspA1I CMGCKG 3 cut(s) 409, 562, 797
MspCI CTTAAG 1 cut(s) 326
MunI CAATTG 1 cut(s) 459
MvnI CGCG 1 cut(s) 865
MwoI GCNNNNNNNGC 3 cut(s) 797, 836, 871
NdeII GATC 6 cut(s) 97, 403, 483, 544, 964, 1077
NlaIII CATG 7 cut(s) 338, 401, 475, 551, 640, 848, 998
NmuCI GTSAC 1 cut(s) 7
NruI TCGCGA 1 cut(s) 865
PagI TCATGA 2 cut(s) 397, 547
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PfeI GAWTC 2 cut(s) 392, 998
PkrI GCNGC 5 cut(s) 43, 411, 529, 564, 799
Ppu21I YACGTR 1 cut(s) 755
PspPI GGNCC 1 cut(s) 331
PsuI RGATCY 1 cut(s) 483
PvuII CAGCTG 1 cut(s) 797
RruI TCGCGA 1 cut(s) 865
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SaqAI TTAA 4 cut(s) 327, 516, 590, 690
SatI GCNGC 5 cut(s) 42, 410, 528, 563, 798
Sau3AI GATC 6 cut(s) 97, 403, 483, 544, 964, 1077
Sau96I GGNCC 1 cut(s) 331
SduI GDGCHC 1 cut(s) 744
SfaNI GCATC 2 cut(s) 262, 839
SfcI CTRYAG 1 cut(s) 792
SinI GGWCC 1 cut(s) 331
SmlI CTYRAG 2 cut(s) 326, 673
SmoI CTYRAG 2 cut(s) 326, 673
Sse9I AATT 4 cut(s) 304, 459, 508, 555
SsiI CCGC 4 cut(s) 42, 275, 407, 560
SspI AATATT 1 cut(s) 904
SspMI CTAG 2 cut(s) 179, 804
TaiI ACGT 2 cut(s) 123, 757
TaqI TCGA 3 cut(s) 270, 342, 1067
TasI AATT 4 cut(s) 304, 459, 508, 555
TatI WGTACW 1 cut(s) 219
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 2 cut(s) 392, 998
Tru1I TTAA 4 cut(s) 327, 516, 590, 690
Tru9I TTAA 4 cut(s) 327, 516, 590, 690
TscAI CASTG 1 cut(s) 846
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 4 cut(s) 409, 527, 562, 797
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 7 cut(s) 351, 576, 625, 861, 998, 1011, 1062
TspGWI ACGGA 1 cut(s) 253
TspRI CASTG 1 cut(s) 846
Vha464I CTTAAG 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 331
XapI RAATTY 1 cut(s) 555
XbaI TCTAGA 1 cut(s) 803
XspI CTAG 2 cut(s) 179, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.