RchiOBHm_Chr6g0287751

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002772
Physical Location & Seq
Reverse (-)
51052640 .. 51054247
1608 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25815

Sequence Viewer

Length: 1407 bp
ATGCCCTCTATAATCTTTATCAGCAATCCGACAAACAACTCAACTCTAGGAGGGTCTGCAATGGAGTCAACAACAGTCCTCTGCTTCCTCTTCTTCTTCATCATCCACACCATCACAGCCTCACCACCACCTCTCCTCCCAAAACAAGCCCTCCCCACCAAGTCAGGCTACCTCCCAGTCAACCCCACCACATCCTCTGCAATATTCTACACCTTCTATGAAGCCCAGAACCCAACTTCACCTCTCTCCCAAACCCCACTCCTCATTTGGCTCCAGGGCGGCCCCGGCTGCTCCTCCATGGTCGGCAACTTCTTCGAGCTCGGTCCCTGGCGCGTTAATTTCCACAAACACCCCTCTGATTCCCTCACCTTGGAACCCAACTCTGGTTCCTGGAACCGCATGTTCGGCTTGGTTTTTCTCGACAATCCCGTCGGAACCGGGTTCAGTATAGCTTCCACACCCGAAGAGATTCCGAGAAACCAGTTTGCGGTTGCACAACACCTGTTCGCTGCAATTACCGAGTTCATTGAGCTTGATCCTGTGTTTAGGTCGAGGCCTCTTTACATCACTGGGGAGAGCTATGCAGGCAAGTATATTCCGGCAATTGGGTACTACATTTTGCAGAGGAATATTGATGCTGAGAGTTCTGGGTCTGATCGGCATGTGAATTTAGCTGGGGTTGCTATAGGAAATGGGGAGACAGACCCGGTGATCCAAGTGAAAACTCATGCTGTAAATGCTTACTTTTCGGGTTTGATCAATGAGAGGCAGAAGAGGGAGGTAGAGGAGCTTCAATTTGAGGCAGTGGATTTGTCTAGAGCTGGGAAATGGAGCGAGGCGAAAATTGCAAGAAGCAGAGTGTTGGATAGGTTGCAAGACATGACCGGGTTGCCCACTTTGTATGATATCAGAAGGAATGCTAGCTATGAAACGCATTTGGTTGGGGAGTTATTGAGGAATGAGGAGGTGAGGAAGGCTTTGGGGGTGTCGAATGAGTCCATAGTTTTCGAGGAATGTAGCCATTTGGTGGGGGAGGTATTGCATGAGGATATGATGAAGAGTGTGAAGTACATGGTGGAAGAGTTGGTGAGGAAGAGCAAGGTTTTGTTGTACCAGGGGCAGTTTGATATGAGATGTGGCGTGGTTGCGAATGAGGCTTGGGTGAAGACCATGAAATGGGAGGGGATAGAGAGGTTTCTGTCGGTGGATAGGAGAGTTTGGAGATTGGGTGGAGAGGTTGCTGGTTATGTTCAGAAATGGGGGAGTCTGAGCTTTGTTTTGGTCTCAGGGGCTGGTCATCTTGTGCCTGCTGACCAGCCATTAAGAGCTCAAGCAATGATTGAAGACTGGGTTTTGGGTAAAGGGTTGTTTGCCAATGTGGAGGAGGATAACCTGTCATCAATATGA

Protein Analysis

468

Amino Acids

52.09

Weight (kDa)

5.58

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 50 - 452 5.1e-103 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 428
AccB7I CCANNNNNTGG 2 cut(s) 1027, 1176
AccII CGCG 1 cut(s) 333
AciI CCGC 3 cut(s) 279, 397, 488
AclWI GGATC 2 cut(s) 530, 706
AcsI RAATTY 1 cut(s) 667
AfaI GTAC 3 cut(s) 611, 1070, 1112
AfiI CCNNNNNNNGG 6 cut(s) 370, 383, 487, 605, 1027, 1176
AgsI TTSAA 2 cut(s) 794, 1343
AjnI CCWGG 4 cut(s) 273, 326, 389, 1113
Alw21I GWGCWC 2 cut(s) 321, 1330
Alw26I GTCTC 2 cut(s) 692, 1288
AlwI GGATC 2 cut(s) 530, 706
AlwNI CAGNNNCTG 1 cut(s) 1292
AoxI GGCC 2 cut(s) 280, 554
ApeKI GCWGC 2 cut(s) 288, 509
ApoI RAATTY 1 cut(s) 667
Asp700I GAANNNNTTC 1 cut(s) 468
AspLEI GCGC 1 cut(s) 333
AspS9I GGNCC 2 cut(s) 281, 323
AsuC2I CCSGG 4 cut(s) 285, 439, 707, 886
AsuHPI GGTGA 7 cut(s) 114, 231, 358, 721, 979, 1099, 1174
AsuNHI GCTAGC 1 cut(s) 920
AvaII GGWCC 1 cut(s) 323
BanII GRGCYC 2 cut(s) 321, 1330
BarI GAAGNNNNNNTAC 2 cut(s) 774, 806
BbsI GAAGAC 2 cut(s) 1172, 1350
Bbv12I GWGCWC 2 cut(s) 321, 1330
BbvI GCAGC 2 cut(s) 275, 496
BccI CCATC 1 cut(s) 119
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 4 cut(s) 275, 328, 391, 1115
BclI TGATCA 1 cut(s) 756
BcnI CCSGG 4 cut(s) 285, 439, 707, 886
BcoDI GTCTC 2 cut(s) 692, 1288
BfaI CTAG 3 cut(s) 47, 816, 921
BfmI CTRYAG 1 cut(s) 684
BisI GCNGC 3 cut(s) 280, 289, 510
BlsI GCNGC 3 cut(s) 281, 290, 511
Bme1390I CCNGG 8 cut(s) 275, 285, 328, 391, 439, 707, 886, 1115
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 281, 323
BmiI GGNNCC 7 cut(s) 272, 283, 325, 375, 388, 395, 436
BmrFI CCNGG 8 cut(s) 275, 285, 328, 391, 439, 707, 886, 1115
BmrI ACTGGG 3 cut(s) 170, 579, 1357
BmsI GCATC 1 cut(s) 625
BmtI GCTAGC 1 cut(s) 924
BmuI ACTGGG 3 cut(s) 170, 579, 1357
BpiI GAAGAC 2 cut(s) 1172, 1350
BpmI CTGGAG 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 1314
BpuMI CCSGG 4 cut(s) 285, 439, 707, 886
BsaI GGTCTC 1 cut(s) 1288
BsaJI CCNNGG 6 cut(s) 274, 283, 297, 326, 369, 1114
BsaXI ACNNNNNCTCC 8 cut(s) 117, 120, 135, 147, 150, 165, 1213, 1243
Bsc4I CCNNNNNNNGG 6 cut(s) 370, 383, 487, 605, 1027, 1176
Bse1I ACTGG 4 cut(s) 176, 481, 574, 1352
Bse3DI GCAATG 2 cut(s) 66, 1341
BseBI CCWGG 4 cut(s) 275, 328, 391, 1115
BseDI CCNNGG 6 cut(s) 274, 283, 297, 326, 369, 1114
BseGI GGATG 2 cut(s) 102, 191
BseLI CCNNNNNNNGG 6 cut(s) 370, 383, 487, 605, 1027, 1176
BseMI GCAATG 2 cut(s) 66, 1341
BseMII CTCAG 3 cut(s) 630, 1259, 1299
BseNI ACTGG 4 cut(s) 176, 481, 574, 1352
BseRI GAGGAG 6 cut(s) 125, 251, 283, 800, 977, 1397
BseXI GCAGC 2 cut(s) 275, 496
BseYI CCCAGC 2 cut(s) 674, 821
Bsh1236I CGCG 1 cut(s) 333
BshFI GGCC 2 cut(s) 282, 556
BsiHKAI GWGCWC 2 cut(s) 321, 1330
BsiSI CCGG 5 cut(s) 285, 438, 599, 707, 885
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 6 cut(s) 370, 383, 487, 605, 1027, 1176
BsmAI GTCTC 2 cut(s) 692, 1288
BsmFI GGGAC 1 cut(s) 309
BsmI GAATGC 1 cut(s) 922
BsnI GGCC 2 cut(s) 282, 556
Bso31I GGTCTC 1 cut(s) 1288
Bsp1286I GDGCHC 2 cut(s) 321, 1330
Bsp143I GATC 4 cut(s) 535, 655, 711, 756
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 3 cut(s) 279, 397, 488
BspANI GGCC 2 cut(s) 282, 556
BspCNI CTCAG 3 cut(s) 631, 1260, 1298
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 7 cut(s) 272, 283, 325, 375, 388, 395, 436
BspOI GCTAGC 1 cut(s) 924
BspPI GGATC 2 cut(s) 530, 706
BspQI GCTCTTC 1 cut(s) 1088
BspTNI GGTCTC 1 cut(s) 1288
BsrDI GCAATG 2 cut(s) 66, 1341
BsrI ACTGG 4 cut(s) 176, 481, 574, 1352
BssECI CCNNGG 6 cut(s) 274, 283, 297, 326, 369, 1114
BssMI GATC 4 cut(s) 535, 655, 711, 756
BssT1I CCWWGG 2 cut(s) 297, 369
Bst2UI CCWGG 4 cut(s) 275, 328, 391, 1115
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 6 cut(s) 95, 459, 767, 1052, 1074, 1088
BstC8I GCNNGC 3 cut(s) 586, 922, 1308
BstDEI CTNAG 3 cut(s) 639, 1268, 1285
BstDSI CCRYGG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 102, 191
BstFNI CGCG 1 cut(s) 333
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 4 cut(s) 538, 658, 714, 759
BstMAI GTCTC 2 cut(s) 692, 1288
BstMBI GATC 4 cut(s) 535, 655, 711, 756
BstMWI GCNNNNNNNGC 8 cut(s) 285, 288, 405, 585, 680, 737, 845, 1154
BstNI CCWGG 4 cut(s) 275, 328, 391, 1115
BstNSI RCATGY 2 cut(s) 403, 665
BstSCI CCNGG 8 cut(s) 273, 283, 326, 389, 437, 705, 884, 1113
BstSFI CTRYAG 1 cut(s) 684
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 275, 496
BstV2I GAAGAC 2 cut(s) 1172, 1350
BsuRI GGCC 2 cut(s) 282, 556
BtgI CCRYGG 1 cut(s) 297
BtsCI GGATG 2 cut(s) 102, 191
BtsI GCAGTG 1 cut(s) 810
BtsIMutI CAGTG 2 cut(s) 567, 810
Cac8I GCNNGC 3 cut(s) 586, 922, 1308
CaiI CAGNNNCTG 1 cut(s) 1292
CfoI GCGC 1 cut(s) 333
Cfr13I GGNCC 2 cut(s) 281, 323
Csp6I GTAC 3 cut(s) 610, 1069, 1111
CviAII CATG 8 cut(s) 298, 400, 662, 728, 880, 1043, 1072, 1171
CviQI GTAC 3 cut(s) 610, 1069, 1111
DdeI CTNAG 3 cut(s) 639, 1268, 1285
DpnI GATC 4 cut(s) 537, 657, 713, 758
DpnII GATC 4 cut(s) 535, 655, 711, 756
DrdI GACNNNNNNGTC 1 cut(s) 428
DseDI GACNNNNNNGTC 1 cut(s) 428
Eam1104I CTCTTC 6 cut(s) 95, 459, 767, 1052, 1074, 1088
EarI CTCTTC 6 cut(s) 95, 459, 767, 1052, 1074, 1088
Ecl136II GAGCTC 2 cut(s) 319, 1328
Eco130I CCWWGG 2 cut(s) 297, 369
Eco147I AGGCCT 1 cut(s) 556
Eco24I GRGCYC 2 cut(s) 321, 1330
Eco31I GGTCTC 1 cut(s) 1288
Eco32I GATATC 1 cut(s) 907
Eco47I GGWCC 1 cut(s) 323
Eco53kI GAGCTC 2 cut(s) 319, 1328
EcoICRI GAGCTC 2 cut(s) 319, 1328
EcoRII CCWGG 4 cut(s) 273, 326, 389, 1113
EcoRV GATATC 1 cut(s) 907
EcoT14I CCWWGG 2 cut(s) 297, 369
EcoT38I GRGCYC 2 cut(s) 321, 1330
ErhI CCWWGG 2 cut(s) 297, 369
FaeI CATG 8 cut(s) 301, 403, 665, 731, 883, 1046, 1075, 1174
FaqI GGGAC 1 cut(s) 309
FatI CATG 8 cut(s) 297, 399, 661, 727, 879, 1042, 1071, 1170
FbaI TGATCA 1 cut(s) 756
Fnu4HI GCNGC 3 cut(s) 280, 289, 510
FokI GGATG 2 cut(s) 89, 178
FriOI GRGCYC 2 cut(s) 321, 1330
Fsp4HI GCNGC 3 cut(s) 280, 289, 510
FspBI CTAG 3 cut(s) 47, 816, 921
GlaI GCGC 1 cut(s) 332
GluI GCNGC 3 cut(s) 280, 289, 510
GsaI CCCAGC 2 cut(s) 678, 825
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 2 cut(s) 282, 556
HapII CCGG 5 cut(s) 285, 438, 599, 707, 885
HhaI GCGC 1 cut(s) 333
Hin1II CATG 8 cut(s) 301, 403, 665, 731, 883, 1046, 1075, 1174
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HincII GTYRAC 2 cut(s) 69, 181
HindII GTYRAC 2 cut(s) 69, 181
HinfI GANTC 5 cut(s) 65, 359, 469, 995, 1264
HpaII CCGG 5 cut(s) 285, 438, 599, 707, 885
HphI GGTGA 7 cut(s) 114, 231, 358, 721, 979, 1099, 1174
Hpy166II GTNNAC 2 cut(s) 69, 181
Hpy188I TCNGA 8 cut(s) 30, 358, 434, 474, 655, 911, 1254, 1269
Hpy188III TCNNGA 2 cut(s) 419, 816
Hpy8I GTNNAC 2 cut(s) 69, 181
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 3 cut(s) 223, 906, 967
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4V TGCA 9 cut(s) 59, 200, 494, 512, 584, 622, 848, 874, 1042
HpyF10VI GCNNNNNNNGC 8 cut(s) 285, 288, 405, 585, 680, 737, 845, 1154
HpyF3I CTNAG 3 cut(s) 639, 1268, 1285
Hsp92II CATG 8 cut(s) 301, 403, 665, 731, 883, 1046, 1075, 1174
HspAI GCGC 1 cut(s) 331
Ksp22I TGATCA 1 cut(s) 756
Kzo9I GATC 4 cut(s) 535, 655, 711, 756
LguI GCTCTTC 1 cut(s) 1088
LmnI GCTCC 4 cut(s) 276, 296, 787, 831
Lsp1109I GCAGC 2 cut(s) 275, 496
LweI GCATC 1 cut(s) 625
MaeI CTAG 3 cut(s) 47, 816, 921
MalI GATC 4 cut(s) 537, 657, 713, 758
MboI GATC 4 cut(s) 535, 655, 711, 756
MfeI CAATTG 1 cut(s) 603
MhlI GDGCHC 2 cut(s) 321, 1330
MluCI AATT 6 cut(s) 337, 513, 603, 667, 794, 843
MlyI GAGTC 3 cut(s) 74, 1004, 1273
MmeI TCCRAC 3 cut(s) 53, 412, 843
MroXI GAANNNNTTC 1 cut(s) 468
MseI TTAA 2 cut(s) 336, 1322
MslI CAYNNNNRTG 1 cut(s) 1402
MspI CCGG 5 cut(s) 285, 438, 599, 707, 885
MspR9I CCNGG 8 cut(s) 275, 285, 328, 391, 439, 707, 886, 1115
MunI CAATTG 1 cut(s) 603
Mva1269I GAATGC 1 cut(s) 922
MvaI CCWGG 4 cut(s) 275, 328, 391, 1115
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 8 cut(s) 285, 288, 405, 585, 680, 737, 845, 1154
NciI CCSGG 4 cut(s) 285, 439, 707, 886
NcoI CCATGG 1 cut(s) 297
NdeII GATC 4 cut(s) 535, 655, 711, 756
NheI GCTAGC 1 cut(s) 920
NlaIII CATG 8 cut(s) 301, 403, 665, 731, 883, 1046, 1075, 1174
NlaIV GGNNCC 7 cut(s) 272, 283, 325, 375, 388, 395, 436
NspI RCATGY 2 cut(s) 403, 665
PceI AGGCCT 1 cut(s) 556
PciSI GCTCTTC 1 cut(s) 1088
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PctI GAATGC 1 cut(s) 922
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 2 cut(s) 359, 469
PflMI CCANNNNNTGG 2 cut(s) 1027, 1176
PfoI TCCNGGA 1 cut(s) 389
PkrI GCNGC 3 cut(s) 281, 290, 511
PleI GAGTC 3 cut(s) 73, 1003, 1272
PpsI GAGTC 3 cut(s) 73, 1003, 1272
Psp124BI GAGCTC 2 cut(s) 321, 1330
Psp6I CCWGG 4 cut(s) 273, 326, 389, 1113
PspFI CCCAGC 2 cut(s) 674, 821
PspGI CCWGG 4 cut(s) 273, 326, 389, 1113
PspN4I GGNNCC 7 cut(s) 272, 283, 325, 375, 388, 395, 436
PspPI GGNCC 2 cut(s) 281, 323
PstNI CAGNNNCTG 1 cut(s) 1292
RsaI GTAC 3 cut(s) 611, 1070, 1112
RsaNI GTAC 3 cut(s) 610, 1069, 1111
RseI CAYNNNNRTG 1 cut(s) 1402
SacI GAGCTC 2 cut(s) 321, 1330
SapI GCTCTTC 1 cut(s) 1088
SaqAI TTAA 2 cut(s) 336, 1322
SatI GCNGC 3 cut(s) 280, 289, 510
Sau3AI GATC 4 cut(s) 535, 655, 711, 756
Sau96I GGNCC 2 cut(s) 281, 323
SchI GAGTC 3 cut(s) 74, 1004, 1273
ScrFI CCNGG 8 cut(s) 275, 285, 328, 391, 439, 707, 886, 1115
SduI GDGCHC 2 cut(s) 321, 1330
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 1 cut(s) 684
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 1402
SmlI CTYRAG 1 cut(s) 1329
SmoI CTYRAG 1 cut(s) 1329
Sse9I AATT 6 cut(s) 337, 513, 603, 667, 794, 843
SseBI AGGCCT 1 cut(s) 556
SsiI CCGC 3 cut(s) 279, 397, 488
SspI AATATT 2 cut(s) 204, 631
SspMI CTAG 3 cut(s) 47, 816, 921
SstI GAGCTC 2 cut(s) 321, 1330
StuI AGGCCT 1 cut(s) 556
StyD4I CCNGG 8 cut(s) 273, 283, 326, 389, 437, 705, 884, 1113
StyI CCWWGG 2 cut(s) 297, 369
TaaI ACNGT 1 cut(s) 76
TaqI TCGA 5 cut(s) 315, 420, 551, 989, 1008
TaqII GACCGA 1 cut(s) 311
TasI AATT 6 cut(s) 337, 513, 603, 667, 794, 843
TatI WGTACW 1 cut(s) 1068
TauI GCSGC 1 cut(s) 282
TfiI GAWTC 2 cut(s) 359, 469
Tru1I TTAA 2 cut(s) 336, 1322
Tru9I TTAA 2 cut(s) 336, 1322
TscAI CASTG 2 cut(s) 574, 810
TseI GCWGC 2 cut(s) 288, 509
TspDTI ATGAA 6 cut(s) 88, 234, 514, 942, 1070, 1187
TspRI CASTG 2 cut(s) 574, 810
Van91I CCANNNNNTGG 2 cut(s) 1027, 1176
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 667
XbaI TCTAGA 1 cut(s) 815
XceI RCATGY 2 cut(s) 403, 665
XcmI CCANNNNNNNNNTGG 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 3 cut(s) 47, 816, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.