RchiOBHm_Chr7g0233381

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007557
Physical Location & Seq
Forward (+)
57786963 .. 57790206
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20919

Sequence Viewer

Length: 396 bp
ATGTCGAAGAGGTTTGGTAATCTGTTGATCGGCAAGACCTACAGTCTCACAGTCCTCCGCCGTAGAATTTATCTGTTTCGCGAAGGGGAGAGGAGCATGATACTTCCGGTGAGCATCCACTGCAACACTTGTGCCAATCGCATGCCCCAAGGCACCAATTTGAACTATGTAAGACGACGCTCCCTCTCCAAGACTTCTTTGGGAGCTCCAATAGTTACATTTTTCTTCAATTGTACCAAGTGCCGGGCACTCATCCGGATGAAGGAAGACCCAGAGAATTCAGGCTATGTTGTTGAGGATGGTGCTACTGAAGTTCTTGAACATGAAAATCAGAACACCTTCGAAAGATTCGTGATGAAGATGGTTTGGATCCTGAAACATCTGACTCTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

131

Amino Acids

15.26

Weight (kDa)

9.83

Isoelectric Point (pI)

56.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 33 - 110 6e-16 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 152
AccII CGCG 1 cut(s) 81
AccIII TCCGGA 1 cut(s) 255
AciI CCGC 1 cut(s) 58
AclWI GGATC 2 cut(s) 364, 377
AcsI RAATTY 2 cut(s) 66, 277
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 2 cut(s) 243, 262
AgsI TTSAA 3 cut(s) 163, 229, 320
AhdI GACNNNNNGTC 1 cut(s) 42
AluBI AGCT 1 cut(s) 206
AluI AGCT 1 cut(s) 206
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 1 cut(s) 50
AlwI GGATC 2 cut(s) 364, 377
Aor13HI TCCGGA 1 cut(s) 255
ApoI RAATTY 2 cut(s) 66, 277
Asp700I GAANNNNTTC 1 cut(s) 338
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 121
AsuII TTCGAA 1 cut(s) 342
BaeGI GKGCMC 1 cut(s) 250
BamHI GGATCC 1 cut(s) 369
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 208
BbsI GAAGAC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 208
BccI CCATC 2 cut(s) 293, 355
BceAI ACGGC 1 cut(s) 45
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 40
Bme1390I CCNGG 1 cut(s) 245
BmeRI GACNNNNNGTC 1 cut(s) 42
BmiI GGNNCC 2 cut(s) 154, 371
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 1 cut(s) 123
BpiI GAAGAC 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 342
BpuMI CCSGG 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 148
BsaWI WCCGGW 2 cut(s) 106, 255
Bsc4I CCNNNNNNNGG 2 cut(s) 243, 262
BseAI TCCGGA 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 148
BseGI GGATG 4 cut(s) 114, 252, 264, 304
BseLI CCNNNNNNNGG 2 cut(s) 243, 262
BseRI GAGGAG 1 cut(s) 106
BseSI GKGCMC 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 81
BshNI GGYRCC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 208
BsiSI CCGG 3 cut(s) 107, 244, 256
BslI CCNNNNNNNGG 2 cut(s) 243, 262
BsmAI GTCTC 1 cut(s) 50
Bsp119I TTCGAA 1 cut(s) 342
Bsp1286I GDGCHC 2 cut(s) 208, 250
Bsp13I TCCGGA 1 cut(s) 255
Bsp143I GATC 2 cut(s) 27, 369
Bsp68I TCGCGA 1 cut(s) 81
BspACI CCGC 1 cut(s) 58
BspEI TCCGGA 1 cut(s) 255
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 154, 371
BspPI GGATC 2 cut(s) 364, 377
BspT104I TTCGAA 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 2 cut(s) 27, 369
BssT1I CCWWGG 1 cut(s) 148
Bst4CI ACNGT 2 cut(s) 44, 52
Bst6I CTCTTC 1 cut(s) 2
BstAPI GCANNNNNTGC 1 cut(s) 120
BstBI TTCGAA 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 143
BstF5I GGATG 4 cut(s) 114, 252, 264, 304
BstFNI CGCG 1 cut(s) 81
BstKTI GATC 2 cut(s) 30, 372
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 2 cut(s) 27, 369
BstMWI GCNNNNNNNGC 1 cut(s) 120
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 243
BstSFI CTRYAG 1 cut(s) 40
BstSLI GKGCMC 1 cut(s) 250
BstUI CGCG 1 cut(s) 81
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 369
BstYI RGATCY 1 cut(s) 369
BtsCI GGATG 4 cut(s) 114, 252, 264, 304
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 118
BtuMI TCGCGA 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 143
CseI GACGC 1 cut(s) 186
Csp6I GTAC 1 cut(s) 234
CviAII CATG 3 cut(s) 97, 142, 323
CviJI RGCY 2 cut(s) 206, 285
CviKI_1 RGCY 2 cut(s) 206, 285
CviQI GTAC 1 cut(s) 234
DpnI GATC 2 cut(s) 29, 371
DpnII GATC 2 cut(s) 27, 369
DriI GACNNNNNGTC 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 2
Eam1105I GACNNNNNGTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 2
EciI GGCGGA 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 206
Eco130I CCWWGG 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 208
Eco53kI GAGCTC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 206
EcoRI GAATTC 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 3 cut(s) 100, 145, 326
FaiI YATR 5 cut(s) 98, 143, 168, 288, 324
FatI CATG 3 cut(s) 96, 141, 322
FokI GGATG 4 cut(s) 101, 239, 271, 311
FriOI GRGCYC 1 cut(s) 208
HapII CCGG 3 cut(s) 107, 244, 256
HgaI GACGC 1 cut(s) 186
Hin1II CATG 3 cut(s) 100, 145, 326
HinfI GANTC 2 cut(s) 348, 385
HpaII CCGG 3 cut(s) 107, 244, 256
HphI GGTGA 1 cut(s) 121
Hpy188I TCNGA 2 cut(s) 333, 384
Hpy188III TCNNGA 5 cut(s) 80, 256, 317, 352, 373
Hpy99I CGWCG 1 cut(s) 180
HpyAV CCTTC 3 cut(s) 77, 256, 349
HpyCH4III ACNGT 2 cut(s) 44, 52
HpyCH4V TGCA 1 cut(s) 123
HpyF10VI GCNNNNNNNGC 1 cut(s) 120
Hsp92II CATG 3 cut(s) 100, 145, 326
Kpn2I TCCGGA 1 cut(s) 255
Kzo9I GATC 2 cut(s) 27, 369
LmnI GCTCC 4 cut(s) 93, 185, 203, 211
LpnPI CCDG 6 cut(s) 120, 257, 267, 269, 285, 386
LweI GCATC 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 214
MalI GATC 2 cut(s) 29, 371
MboI GATC 2 cut(s) 27, 369
MboII GAAGA 4 cut(s) 19, 217, 278, 370
MfeI CAATTG 1 cut(s) 229
MflI RGATCY 1 cut(s) 369
MhlI GDGCHC 2 cut(s) 208, 250
MluCI AATT 5 cut(s) 66, 157, 229, 277, 390
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 5 cut(s) 3, 65, 84, 194, 289
MroI TCCGGA 1 cut(s) 255
MroXI GAANNNNTTC 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 257
MspI CCGG 3 cut(s) 107, 244, 256
MspR9I CCNGG 1 cut(s) 245
MunI CAATTG 1 cut(s) 229
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 1 cut(s) 120
NciI CCSGG 1 cut(s) 245
NdeII GATC 2 cut(s) 27, 369
NlaIII CATG 3 cut(s) 100, 145, 326
NlaIV GGNNCC 2 cut(s) 154, 371
NruI TCGCGA 1 cut(s) 81
NspI RCATGY 1 cut(s) 145
NspV TTCGAA 1 cut(s) 342
PaeI GCATGC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 1 cut(s) 348
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 208
PspN4I GGNNCC 2 cut(s) 154, 371
PsuI RGATCY 1 cut(s) 369
RruI TCGCGA 1 cut(s) 81
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
RseI CAYNNNNRTG 1 cut(s) 257
SacI GAGCTC 1 cut(s) 208
Sau3AI GATC 2 cut(s) 27, 369
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 2 cut(s) 208, 250
SetI ASST 4 cut(s) 14, 41, 208, 341
SfaNI GCATC 1 cut(s) 123
SfcI CTRYAG 1 cut(s) 40
SfuI TTCGAA 1 cut(s) 342
SmiMI CAYNNNNRTG 1 cut(s) 257
SphI GCATGC 1 cut(s) 145
Sse9I AATT 5 cut(s) 66, 157, 229, 277, 390
SsiI CCGC 1 cut(s) 58
SstI GAGCTC 1 cut(s) 208
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 2 cut(s) 44, 52
TaqI TCGA 2 cut(s) 5, 342
TasI AATT 5 cut(s) 66, 157, 229, 277, 390
TfiI GAWTC 1 cut(s) 348
TscAI CASTG 1 cut(s) 125
TspDTI ATGAA 3 cut(s) 275, 339, 371
TspRI CASTG 1 cut(s) 125
XapI RAATTY 2 cut(s) 66, 277
XceI RCATGY 1 cut(s) 145
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XmnI GAANNNNTTC 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.