Rw7G035970

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007557
Physical Location & Seq
Forward (+)
54059820 .. 54062179
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035970.1

Sequence Viewer

Length: 630 bp
ATGAAGTTCTGCAATATATTGATCGGCAAGCCCTACAGTCCCGCTCCCGGTTTCCACGTACTCCGCCATAGAATTTACCGGTTCGGCGAAGGAGAGACGAGGATGTTACCTCTGAAGAGCATGATACTTCCGGTGAGCATCCGCTGCAGCACTTGTGCCAATCACATTGCCCAGGGCACCACTTTGACCTATGTAAGACGGCACGCCTTCTCCGAGACTTCTTTGGGAGCTCCAATAGTAACATTTGTCTTCAATTGTACCAAGTGCCGGGCACTCATCCAGATGAAGGAAGACCCAGAGCACTCAGGCTATGTTGTGGAGGATGGTGCTACAAAATTTTTTGATGAACATGAGAAATCAGAACACCGTGGCAAGATTAGTGATGAAGATGGTTTGGATCATGAAACATCTGACTCTAATTTGAAGAAATCTGGACATTGTCGAAAGATTCGTGATGAAGATGGTTTGGATCATGAAACATCCTACTCTAATTTGAAGAGACAAAAGCTTATGATTGGGCTTCCTAGCAACCCAACAGAGTCGTTCACTCTTTCGGATAGCTCAAGCACAGAGCTAGTCTTTCCCCATGTGGGCAATGAGAATTTCGATTGTGAATTGCTCTCGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

209

Amino Acids

23.59

Weight (kDa)

6.41

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 41 - 133 8.5e-21 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AccBSI CCGCTC 1 cut(s) 44
AciI CCGC 3 cut(s) 42, 64, 142
AclWI GGATC 2 cut(s) 405, 477
AcsI RAATTY 3 cut(s) 72, 335, 601
AcuI CTGAAG 1 cut(s) 134
AfaI GTAC 2 cut(s) 60, 259
AfiI CCNNNNNNNGG 4 cut(s) 47, 267, 286, 590
AgeI ACCGGT 1 cut(s) 78
AgsI TTSAA 3 cut(s) 253, 424, 496
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 4 cut(s) 230, 508, 561, 574
AluI AGCT 4 cut(s) 230, 508, 561, 574
Alw21I GWGCWC 2 cut(s) 232, 303
Alw26I GTCTC 3 cut(s) 89, 209, 493
AlwI GGATC 2 cut(s) 405, 477
Ama87I CYCGRG 1 cut(s) 622
ApeKI GCWGC 2 cut(s) 144, 147
ApoI RAATTY 3 cut(s) 72, 335, 601
AsiGI ACCGGT 1 cut(s) 78
AsuC2I CCSGG 2 cut(s) 48, 269
AsuHPI GGTGA 1 cut(s) 145
AvaI CYCGRG 1 cut(s) 622
BaeGI GKGCMC 2 cut(s) 179, 274
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 232
BbsI GAAGAC 2 cut(s) 241, 297
Bbv12I GWGCWC 2 cut(s) 232, 303
BbvI GCAGC 2 cut(s) 131, 159
BccI CCATC 3 cut(s) 317, 383, 455
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 173
BcnI CCSGG 2 cut(s) 48, 269
BcoDI GTCTC 3 cut(s) 89, 209, 493
BfaI CTAG 2 cut(s) 525, 575
BfmI CTRYAG 2 cut(s) 34, 145
BisI GCNGC 2 cut(s) 145, 148
BlsI GCNGC 2 cut(s) 146, 149
Bme1390I CCNGG 3 cut(s) 48, 173, 269
BmeT110I CYCGRG 1 cut(s) 622
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 3 cut(s) 48, 173, 269
BmsI GCATC 1 cut(s) 147
BpiI GAAGAC 2 cut(s) 241, 297
BpuEI CTTGAG 1 cut(s) 547
BpuMI CCSGG 2 cut(s) 48, 269
BsaAI YACGTR 1 cut(s) 58
BsaJI CCNNGG 3 cut(s) 171, 172, 367
BsaWI WCCGGW 2 cut(s) 78, 130
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 267, 286, 590
Bse118I RCCGGY 1 cut(s) 78
Bse3DI GCAATG 2 cut(s) 165, 601
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 3 cut(s) 171, 172, 367
BseGI GGATG 5 cut(s) 108, 138, 276, 328, 479
BseLI CCNNNNNNNGG 4 cut(s) 47, 267, 286, 590
BseMI GCAATG 2 cut(s) 165, 601
BseMII CTCAG 1 cut(s) 318
BseSI GKGCMC 2 cut(s) 179, 274
BseXI GCAGC 2 cut(s) 131, 159
BshNI GGYRCC 1 cut(s) 176
BshTI ACCGGT 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 232, 303
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 4 cut(s) 48, 79, 131, 268
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 4 cut(s) 47, 267, 286, 590
BsmAI GTCTC 3 cut(s) 89, 209, 493
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 622
Bsp1286I GDGCHC 4 cut(s) 179, 232, 274, 303
Bsp143I GATC 3 cut(s) 21, 397, 469
BspACI CCGC 3 cut(s) 42, 64, 142
BspCNI CTCAG 1 cut(s) 317
BspHI TCATGA 2 cut(s) 400, 472
BspLI GGNNCC 1 cut(s) 178
BspMAI CTGCAG 1 cut(s) 149
BspPI GGATC 2 cut(s) 405, 477
BspQI GCTCTTC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 176
BsrBI CCGCTC 1 cut(s) 44
BsrDI GCAATG 2 cut(s) 165, 601
BsrFI RCCGGY 1 cut(s) 78
BssAI RCCGGY 1 cut(s) 78
BssECI CCNNGG 3 cut(s) 171, 172, 367
BssMI GATC 3 cut(s) 21, 397, 469
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 2 cut(s) 38, 368
Bst6I CTCTTC 2 cut(s) 110, 491
BstAPI GCANNNNNTGC 1 cut(s) 144
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 2 cut(s) 29, 204
BstDEI CTNAG 1 cut(s) 304
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 5 cut(s) 108, 138, 276, 328, 479
BstKTI GATC 3 cut(s) 24, 400, 472
BstMAI GTCTC 3 cut(s) 89, 209, 493
BstMBI GATC 3 cut(s) 21, 397, 469
BstMWI GCNNNNNNNGC 1 cut(s) 144
BstNI CCWGG 1 cut(s) 173
BstSCI CCNGG 3 cut(s) 46, 171, 267
BstSFI CTRYAG 2 cut(s) 34, 145
BstSLI GKGCMC 2 cut(s) 179, 274
BstV1I GCAGC 2 cut(s) 131, 159
BstV2I GAAGAC 2 cut(s) 241, 297
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 5 cut(s) 108, 138, 276, 328, 479
Cac8I GCNNGC 2 cut(s) 29, 204
CciI TCATGA 2 cut(s) 400, 472
Cfr10I RCCGGY 1 cut(s) 78
Csp6I GTAC 2 cut(s) 59, 258
CspAI ACCGGT 1 cut(s) 78
CviAII CATG 5 cut(s) 121, 350, 401, 473, 587
CviJI RGCY 7 cut(s) 31, 230, 309, 508, 520, 561, 574
CviKI_1 RGCY 7 cut(s) 31, 230, 309, 508, 520, 561, 574
CviQI GTAC 2 cut(s) 59, 258
DdeI CTNAG 1 cut(s) 304
DpnI GATC 3 cut(s) 23, 399, 471
DpnII GATC 3 cut(s) 21, 397, 469
Eam1104I CTCTTC 2 cut(s) 110, 491
EarI CTCTTC 2 cut(s) 110, 491
EciI GGCGGA 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 230
Eco24I GRGCYC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 622
EcoICRI GAGCTC 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 89
FaeI CATG 5 cut(s) 124, 353, 404, 476, 590
FaqI GGGAC 1 cut(s) 24
FatI CATG 5 cut(s) 120, 349, 400, 472, 586
FauI CCCGC 1 cut(s) 49
Fnu4HI GCNGC 2 cut(s) 145, 148
FokI GGATG 5 cut(s) 115, 125, 263, 335, 466
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 2 cut(s) 145, 148
FspBI CTAG 2 cut(s) 525, 575
GluI GCNGC 2 cut(s) 145, 148
HapII CCGG 4 cut(s) 48, 79, 131, 268
Hin1II CATG 5 cut(s) 124, 353, 404, 476, 590
HindIII AAGCTT 1 cut(s) 506
HinfI GANTC 3 cut(s) 413, 448, 539
HpaII CCGG 4 cut(s) 48, 79, 131, 268
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 1 cut(s) 546
Hpy188I TCNGA 5 cut(s) 114, 214, 361, 412, 556
Hpy188III TCNNGA 7 cut(s) 280, 401, 432, 452, 473, 622, 624
Hpy8I GTNNAC 1 cut(s) 546
HpyAV CCTTC 3 cut(s) 83, 217, 280
HpyCH4III ACNGT 2 cut(s) 38, 368
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 2 cut(s) 12, 147
HpyF10VI GCNNNNNNNGC 1 cut(s) 144
HpyF3I CTNAG 1 cut(s) 304
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 5 cut(s) 124, 353, 404, 476, 590
Kzo9I GATC 3 cut(s) 21, 397, 469
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 3 cut(s) 49, 227, 235
Lsp1109I GCAGC 2 cut(s) 131, 159
LweI GCATC 1 cut(s) 147
MaeI CTAG 2 cut(s) 525, 575
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 2 cut(s) 105, 238
MalI GATC 3 cut(s) 23, 399, 471
MbiI CCGCTC 1 cut(s) 44
MboI GATC 3 cut(s) 21, 397, 469
MboII GAAGA 7 cut(s) 127, 241, 302, 398, 436, 470, 508
MfeI CAATTG 1 cut(s) 253
MhlI GDGCHC 4 cut(s) 179, 232, 274, 303
MluCI AATT 7 cut(s) 72, 253, 335, 418, 490, 601, 614
MlyI GAGTC 2 cut(s) 407, 548
MnlI CCTC 3 cut(s) 93, 120, 313
MslI CAYNNNNRTG 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 144
MspI CCGG 4 cut(s) 48, 79, 131, 268
MspR9I CCNGG 3 cut(s) 48, 173, 269
MunI CAATTG 1 cut(s) 253
MvaI CCWGG 1 cut(s) 173
MwoI GCNNNNNNNGC 1 cut(s) 144
NciI CCSGG 2 cut(s) 48, 269
NdeII GATC 3 cut(s) 21, 397, 469
NlaIII CATG 5 cut(s) 124, 353, 404, 476, 590
NlaIV GGNNCC 1 cut(s) 178
PaeR7I CTCGAG 1 cut(s) 622
PagI TCATGA 2 cut(s) 400, 472
PasI CCCWGGG 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 110
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 448
PfeI GAWTC 1 cut(s) 448
PflFI GACNNNGTC 1 cut(s) 438
PinAI ACCGGT 1 cut(s) 78
PkrI GCNGC 2 cut(s) 146, 149
PleI GAGTC 2 cut(s) 407, 547
PpsI GAGTC 2 cut(s) 407, 547
Ppu21I YACGTR 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 171
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 1 cut(s) 178
PstI CTGCAG 1 cut(s) 149
PsyI GACNNNGTC 1 cut(s) 438
RsaI GTAC 2 cut(s) 60, 259
RsaNI GTAC 2 cut(s) 59, 258
RseI CAYNNNNRTG 1 cut(s) 281
SacI GAGCTC 1 cut(s) 232
SapI GCTCTTC 1 cut(s) 110
SatI GCNGC 2 cut(s) 145, 148
Sau3AI GATC 3 cut(s) 21, 397, 469
SchI GAGTC 2 cut(s) 407, 548
ScrFI CCNGG 3 cut(s) 48, 173, 269
SduI GDGCHC 4 cut(s) 179, 232, 274, 303
SetI ASST 7 cut(s) 60, 112, 191, 232, 510, 563, 576
SfaNI GCATC 1 cut(s) 147
SfcI CTRYAG 2 cut(s) 34, 145
Sfr274I CTCGAG 1 cut(s) 622
SlaI CTCGAG 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 2 cut(s) 562, 622
SmoI CTYRAG 2 cut(s) 562, 622
Sse9I AATT 7 cut(s) 72, 253, 335, 418, 490, 601, 614
SsiI CCGC 3 cut(s) 42, 64, 142
SspMI CTAG 2 cut(s) 525, 575
SstI GAGCTC 1 cut(s) 232
StyD4I CCNGG 3 cut(s) 46, 171, 267
TaaI ACNGT 2 cut(s) 38, 368
TaiI ACGT 1 cut(s) 60
TaqI TCGA 3 cut(s) 442, 606, 623
TasI AATT 7 cut(s) 72, 253, 335, 418, 490, 601, 614
TfiI GAWTC 1 cut(s) 448
TseI GCWGC 2 cut(s) 144, 147
TspDTI ATGAA 7 cut(s) 17, 299, 360, 399, 417, 471, 489
Tth111I GACNNNGTC 1 cut(s) 438
XapI RAATTY 3 cut(s) 72, 335, 601
XhoI CTCGAG 1 cut(s) 622
XspI CTAG 2 cut(s) 525, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.