Rh3DG121100

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0007557
Physical Location & Seq
Forward (+)
10267510 .. 10267953
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG121100.1

Sequence Viewer

Length: 444 bp
ATGTTGGTCGACAAGCCCTACGGTCTCACTACAGGCGTCAATGTAATCCGCCATAGAATTTACCGGTTCCGGGAAGGAGAGAAGAGGTGGTTACAGCTGAAGAGCATGATACTTCCGGTGAGCATCCGATGCACCACTTGTGTCAATCACATGCCCCAAGGCACCACTTTGACCTATGTAAGACGACGCGCCCTCTCCAAGACTTCTTTGGGAGCTCCAATAGTTACATTTTTCTTCAATGCAAATATCCAGATGAAGGAAGACCCAGAGAATTCAGGCCATGTTGTTGAGGATGGTGCTACAGAAATTTTTGAACATGAGAAATCAGAACACCTTGGAAAGATTGGTGATGAAGATGGTTTGGATCATGAAACATCTGACTCTAATTTGAAGAAATCAGGGCATCGTAAGATTCGTGATATGAAGCGTCTGACTCTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

147

Amino Acids

16.84

Weight (kDa)

9.17

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 36 - 131 3.8e-10 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccI GTMKAC 1 cut(s) 9
AccII CGCG 1 cut(s) 189
AciI CCGC 1 cut(s) 49
AclWI GGATC 1 cut(s) 372
AcsI RAATTY 3 cut(s) 57, 271, 306
AcuI CTGAAG 1 cut(s) 119
AcyI GRCGYC 1 cut(s) 36
AfiI CCNNNNNNNGG 2 cut(s) 70, 256
AgeI ACCGGT 1 cut(s) 63
AgsI TTSAA 3 cut(s) 238, 314, 391
AluBI AGCT 2 cut(s) 97, 215
AluI AGCT 2 cut(s) 97, 215
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 372
AoxI GGCC 1 cut(s) 277
ApoI RAATTY 3 cut(s) 57, 271, 306
AsiGI ACCGGT 1 cut(s) 63
AspLEI GCGC 1 cut(s) 191
AsuC2I CCSGG 1 cut(s) 71
AsuHPI GGTGA 2 cut(s) 130, 359
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 217
BccI CCATC 2 cut(s) 287, 350
BcnI CCSGG 1 cut(s) 71
BcoDI GTCTC 1 cut(s) 29
BfmI CTRYAG 2 cut(s) 30, 300
Bme1390I CCNGG 1 cut(s) 71
BmiI GGNNCC 2 cut(s) 68, 163
BmrFI CCNGG 1 cut(s) 71
BmsI GCATC 3 cut(s) 119, 132, 412
BpiI GAAGAC 1 cut(s) 267
BpuMI CCSGG 1 cut(s) 71
BsaHI GRCGYC 1 cut(s) 36
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 157, 334
BsaWI WCCGGW 2 cut(s) 63, 115
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 256
Bse118I RCCGGY 1 cut(s) 63
BseDI CCNNGG 2 cut(s) 157, 334
BseGI GGATG 2 cut(s) 123, 298
BseLI CCNNNNNNNGG 2 cut(s) 70, 256
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 1 cut(s) 279
BshNI GGYRCC 1 cut(s) 161
BshTI ACCGGT 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 3 cut(s) 64, 70, 116
BslI CCNNNNNNNGG 2 cut(s) 70, 256
BsmAI GTCTC 1 cut(s) 29
BsnI GGCC 1 cut(s) 279
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 1 cut(s) 364
BspACI CCGC 1 cut(s) 49
BspANI GGCC 1 cut(s) 279
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 68, 163
BspPI GGATC 1 cut(s) 372
BspQI GCTCTTC 1 cut(s) 95
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 29
BsrFI RCCGGY 1 cut(s) 63
BssAI RCCGGY 1 cut(s) 63
BssECI CCNNGG 2 cut(s) 157, 334
BssMI GATC 1 cut(s) 364
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 2 cut(s) 157, 334
Bst4CI ACNGT 1 cut(s) 23
Bst6I CTCTTC 2 cut(s) 77, 95
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 129
BstF5I GGATG 2 cut(s) 123, 298
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 1 cut(s) 367
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 1 cut(s) 364
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 69
BstSFI CTRYAG 2 cut(s) 30, 300
BstUI CGCG 1 cut(s) 189
BstV2I GAAGAC 1 cut(s) 267
BsuRI GGCC 1 cut(s) 279
BtsCI GGATG 2 cut(s) 123, 298
CciI TCATGA 1 cut(s) 367
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 63
CseI GACGC 3 cut(s) 25, 195, 416
CspAI ACCGGT 1 cut(s) 63
CviAII CATG 5 cut(s) 106, 151, 281, 317, 368
CviJI RGCY 4 cut(s) 16, 97, 215, 279
CviKI_1 RGCY 4 cut(s) 16, 97, 215, 279
DpnI GATC 1 cut(s) 366
DpnII GATC 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 77, 95
EarI CTCTTC 2 cut(s) 77, 95
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 157, 334
Eco24I GRGCYC 1 cut(s) 217
Eco31I GGTCTC 1 cut(s) 29
Eco53kI GAGCTC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 271
EcoT14I CCWWGG 2 cut(s) 157, 334
EcoT38I GRGCYC 1 cut(s) 217
ErhI CCWWGG 2 cut(s) 157, 334
FaeI CATG 5 cut(s) 109, 154, 284, 320, 371
FaiI YATR 8 cut(s) 54, 107, 152, 177, 282, 318, 369, 422
FatI CATG 5 cut(s) 105, 150, 280, 316, 367
FblI GTMKAC 1 cut(s) 9
FokI GGATG 2 cut(s) 110, 305
FriOI GRGCYC 1 cut(s) 217
GlaI GCGC 1 cut(s) 190
HaeIII GGCC 1 cut(s) 279
HapII CCGG 3 cut(s) 64, 70, 116
HgaI GACGC 3 cut(s) 25, 195, 416
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 5 cut(s) 109, 154, 284, 320, 371
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HinfI GANTC 3 cut(s) 380, 412, 433
HpaII CCGG 3 cut(s) 64, 70, 116
HphI GGTGA 2 cut(s) 130, 359
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 5 cut(s) 128, 328, 379, 432, 438
Hpy188III TCNNGA 3 cut(s) 250, 368, 416
Hpy8I GTNNAC 1 cut(s) 10
Hpy99I CGWCG 1 cut(s) 189
HpyAV CCTTC 2 cut(s) 68, 250
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4V TGCA 2 cut(s) 132, 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 5 cut(s) 109, 154, 284, 320, 371
HspAI GCGC 1 cut(s) 189
Kzo9I GATC 1 cut(s) 364
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 2 cut(s) 212, 220
LpnPI CCDG 8 cut(s) 18, 77, 83, 129, 261, 263, 279, 384
LweI GCATC 3 cut(s) 119, 132, 412
MaeIII GTNAC 2 cut(s) 90, 223
MalI GATC 1 cut(s) 366
MboI GATC 1 cut(s) 364
MboII GAAGA 6 cut(s) 94, 112, 226, 272, 365, 403
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 4 cut(s) 57, 271, 306, 385
MlyI GAGTC 2 cut(s) 374, 427
MnlI CCTC 3 cut(s) 78, 203, 283
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 3 cut(s) 64, 70, 116
MspR9I CCNGG 1 cut(s) 71
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 71
NdeII GATC 1 cut(s) 364
NlaIII CATG 5 cut(s) 109, 154, 284, 320, 371
NlaIV GGNNCC 2 cut(s) 68, 163
NspI RCATGY 1 cut(s) 154
PagI TCATGA 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PfeI GAWTC 1 cut(s) 412
PfoI TCCNGGA 1 cut(s) 69
PinAI ACCGGT 1 cut(s) 63
PleI GAGTC 2 cut(s) 374, 427
PpsI GAGTC 2 cut(s) 374, 427
Psp124BI GAGCTC 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 68, 163
PvuII CAGCTG 1 cut(s) 97
SacI GAGCTC 1 cut(s) 217
SalI GTCGAC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 95
Sau3AI GATC 1 cut(s) 364
SchI GAGTC 2 cut(s) 374, 427
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 1 cut(s) 217
SetI ASST 5 cut(s) 89, 99, 176, 217, 336
SfaNI GCATC 3 cut(s) 119, 132, 412
SfcI CTRYAG 2 cut(s) 30, 300
Sse9I AATT 4 cut(s) 57, 271, 306, 385
SsiI CCGC 1 cut(s) 49
SstI GAGCTC 1 cut(s) 217
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 2 cut(s) 157, 334
TaaI ACNGT 1 cut(s) 23
TaqI TCGA 1 cut(s) 9
TasI AATT 4 cut(s) 57, 271, 306, 385
TfiI GAWTC 1 cut(s) 412
TspDTI ATGAA 4 cut(s) 269, 366, 384, 437
XapI RAATTY 3 cut(s) 57, 271, 306
XceI RCATGY 1 cut(s) 154
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XmiI GTMKAC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.