RchiOBHm_Chr7g0233401

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007557
Physical Location & Seq
Forward (+)
57809939 .. 57812125
2187 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20921

Sequence Viewer

Length: 591 bp
ATGAAGTTCTGCAATATATTGATCGGCAAGCCCTACAGTCCCGCTCCCAGTTTCCACGTACTTCGCCATAGAATTTACCTGTTCGGCGAAGGAGAGACGAGGGTGTTACCACTGAAGAGCATGATACTTCCGGTGAGCATCGGCTGCAGCACTTGTGCCAATCACATTGCCCAGGGCACCACTTTGACCTATGTAAAACGGCACGGCTTCTCCGAGACTTCTTTGGGAGCTCCAATAGTAACATTTGTCTTCAATTGTACCAAGTGCCGGGCACTCATCCAGATGAAGGAAGACCCAGAGAACTCAGGCTATGTTGTTGAGGATGGTGCTACAAATTTTTTTGATGAACATGAGAAATCAGAACACCGTGGCAAGATTAGTGATGAAGATGGTTTGGATCATGAAACATCTGACTCTAATTTGAAGAAATTTGGACATTATTGTCGAAAGATTCGTGATGAAGATGGTTTGGATCATGAAACATCCTACTCTAATTTGAAGAGACAAAAGTTTATGATTGGGCTTCCCAGCAACCCGACAGACGTGTTCACTATTTCGGATAGCTCAAGCACAGAGGTATGCTCGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

196

Amino Acids

21.98

Weight (kDa)

6.19

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 41 - 135 1.2e-19 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 176
AccBSI CCGCTC 1 cut(s) 44
AciI CCGC 1 cut(s) 42
AclWI GGATC 2 cut(s) 405, 480
AcsI RAATTY 3 cut(s) 72, 334, 428
AcuI CTGAAG 1 cut(s) 134
AfaI GTAC 2 cut(s) 60, 259
AfiI CCNNNNNNNGG 2 cut(s) 267, 286
AflIII ACRYGT 1 cut(s) 543
AgsI TTSAA 3 cut(s) 253, 424, 499
AjiI CACGTC 1 cut(s) 544
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 2 cut(s) 230, 564
AluI AGCT 2 cut(s) 230, 564
Alw21I GWGCWC 1 cut(s) 232
Alw26I GTCTC 3 cut(s) 89, 209, 496
AlwI GGATC 2 cut(s) 405, 480
ApeKI GCWGC 2 cut(s) 144, 147
ApoI RAATTY 3 cut(s) 72, 334, 428
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 145
BaeGI GKGCMC 2 cut(s) 179, 274
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 232
BbsI GAAGAC 2 cut(s) 241, 297
Bbv12I GWGCWC 1 cut(s) 232
BbvI GCAGC 2 cut(s) 131, 159
BccI CCATC 3 cut(s) 317, 383, 458
BceAI ACGGC 2 cut(s) 215, 220
BciT130I CCWGG 1 cut(s) 173
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 3 cut(s) 89, 209, 496
BfmI CTRYAG 2 cut(s) 34, 145
BisI GCNGC 2 cut(s) 145, 148
BlsI GCNGC 2 cut(s) 146, 149
Bme1390I CCNGG 2 cut(s) 173, 269
BmgBI CACGTC 1 cut(s) 544
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 2 cut(s) 173, 269
BmrI ACTGGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 147
BmuI ACTGGG 1 cut(s) 42
BpiI GAAGAC 2 cut(s) 241, 297
BplI GAGNNNNNCTC 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 550
BpuMI CCSGG 1 cut(s) 269
BsaAI YACGTR 1 cut(s) 58
BsaJI CCNNGG 3 cut(s) 171, 172, 367
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 267, 286
Bse1I ACTGG 1 cut(s) 48
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 3 cut(s) 171, 172, 367
BseGI GGATG 3 cut(s) 276, 328, 482
BseLI CCNNNNNNNGG 2 cut(s) 267, 286
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 1 cut(s) 48
BseSI GKGCMC 2 cut(s) 179, 274
BseXI GCAGC 2 cut(s) 131, 159
BseYI CCCAGC 1 cut(s) 527
BshNI GGYRCC 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 232
BsiSI CCGG 2 cut(s) 131, 268
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 2 cut(s) 267, 286
BsmAI GTCTC 3 cut(s) 89, 209, 496
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 24
Bsp1286I GDGCHC 3 cut(s) 179, 232, 274
Bsp143I GATC 3 cut(s) 21, 397, 472
BspACI CCGC 1 cut(s) 42
BspCNI CTCAG 1 cut(s) 317
BspHI TCATGA 2 cut(s) 400, 475
BspLI GGNNCC 1 cut(s) 178
BspMAI CTGCAG 1 cut(s) 149
BspPI GGATC 2 cut(s) 405, 480
BspQI GCTCTTC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 176
BsrBI CCGCTC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 165
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 3 cut(s) 171, 172, 367
BssMI GATC 3 cut(s) 21, 397, 472
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 2 cut(s) 38, 368
Bst6I CTCTTC 2 cut(s) 110, 494
BstAPI GCANNNNNTGC 1 cut(s) 144
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 2 cut(s) 29, 584
BstDEI CTNAG 1 cut(s) 304
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 3 cut(s) 276, 328, 482
BstKTI GATC 3 cut(s) 24, 400, 475
BstMAI GTCTC 3 cut(s) 89, 209, 496
BstMBI GATC 3 cut(s) 21, 397, 472
BstMWI GCNNNNNNNGC 1 cut(s) 144
BstNI CCWGG 1 cut(s) 173
BstSCI CCNGG 2 cut(s) 171, 267
BstSFI CTRYAG 2 cut(s) 34, 145
BstSLI GKGCMC 2 cut(s) 179, 274
BstV1I GCAGC 2 cut(s) 131, 159
BstV2I GAAGAC 2 cut(s) 241, 297
BtgI CCRYGG 1 cut(s) 367
BtrI CACGTC 1 cut(s) 544
BtsCI GGATG 3 cut(s) 276, 328, 482
BtsIMutI CAGTG 2 cut(s) 110, 586
Cac8I GCNNGC 2 cut(s) 29, 584
CciI TCATGA 2 cut(s) 400, 475
Csp6I GTAC 2 cut(s) 59, 258
CviAII CATG 4 cut(s) 121, 350, 401, 476
CviJI RGCY 7 cut(s) 31, 144, 207, 230, 309, 523, 564
CviKI_1 RGCY 7 cut(s) 31, 144, 207, 230, 309, 523, 564
CviQI GTAC 2 cut(s) 59, 258
DdeI CTNAG 1 cut(s) 304
DpnI GATC 3 cut(s) 23, 399, 474
DpnII GATC 3 cut(s) 21, 397, 472
DrdI GACNNNNNNGTC 1 cut(s) 441
DseDI GACNNNNNNGTC 1 cut(s) 441
Eam1104I CTCTTC 2 cut(s) 110, 494
EarI CTCTTC 2 cut(s) 110, 494
Ecl136II GAGCTC 1 cut(s) 230
Eco24I GRGCYC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 89
FaeI CATG 4 cut(s) 124, 353, 404, 479
FaqI GGGAC 1 cut(s) 24
FatI CATG 4 cut(s) 120, 349, 400, 475
FauI CCCGC 1 cut(s) 49
Fnu4HI GCNGC 2 cut(s) 145, 148
FokI GGATG 3 cut(s) 263, 335, 469
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 2 cut(s) 145, 148
GluI GCNGC 2 cut(s) 145, 148
GsaI CCCAGC 1 cut(s) 531
HapII CCGG 2 cut(s) 131, 268
Hin1II CATG 4 cut(s) 124, 353, 404, 479
HinfI GANTC 2 cut(s) 413, 451
HpaII CCGG 2 cut(s) 131, 268
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 1 cut(s) 549
Hpy188I TCNGA 4 cut(s) 214, 361, 412, 559
Hpy188III TCNNGA 4 cut(s) 280, 401, 455, 476
Hpy8I GTNNAC 1 cut(s) 549
HpyAV CCTTC 2 cut(s) 83, 280
HpyCH4III ACNGT 2 cut(s) 38, 368
HpyCH4IV ACGT 2 cut(s) 57, 543
HpyCH4V TGCA 2 cut(s) 12, 147
HpyF10VI GCNNNNNNNGC 1 cut(s) 144
HpyF3I CTNAG 1 cut(s) 304
HpySE526I ACGT 2 cut(s) 57, 543
Hsp92II CATG 4 cut(s) 124, 353, 404, 479
Kzo9I GATC 3 cut(s) 21, 397, 472
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 3 cut(s) 49, 227, 235
Lsp1109I GCAGC 2 cut(s) 131, 159
LweI GCATC 1 cut(s) 147
MaeII ACGT 2 cut(s) 57, 543
MaeIII GTNAC 2 cut(s) 105, 238
MalI GATC 3 cut(s) 23, 399, 474
MbiI CCGCTC 1 cut(s) 44
MboI GATC 3 cut(s) 21, 397, 472
MboII GAAGA 7 cut(s) 127, 241, 302, 398, 436, 473, 511
MfeI CAATTG 1 cut(s) 253
MhlI GDGCHC 3 cut(s) 179, 232, 274
MluCI AATT 6 cut(s) 72, 253, 334, 418, 428, 493
MlyI GAGTC 1 cut(s) 407
MnlI CCTC 3 cut(s) 93, 313, 568
MslI CAYNNNNRTG 1 cut(s) 281
MspI CCGG 2 cut(s) 131, 268
MspR9I CCNGG 2 cut(s) 173, 269
MunI CAATTG 1 cut(s) 253
MvaI CCWGG 1 cut(s) 173
MwoI GCNNNNNNNGC 1 cut(s) 144
NciI CCSGG 1 cut(s) 269
NdeII GATC 3 cut(s) 21, 397, 472
NlaIII CATG 4 cut(s) 124, 353, 404, 479
NlaIV GGNNCC 1 cut(s) 178
PagI TCATGA 2 cut(s) 400, 475
PasI CCCWGGG 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 110
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 451
PfeI GAWTC 1 cut(s) 451
PkrI GCNGC 2 cut(s) 146, 149
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
Ppu21I YACGTR 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 171
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 1 cut(s) 178
PstI CTGCAG 1 cut(s) 149
RsaI GTAC 2 cut(s) 60, 259
RsaNI GTAC 2 cut(s) 59, 258
RseI CAYNNNNRTG 1 cut(s) 281
SacI GAGCTC 1 cut(s) 232
SapI GCTCTTC 1 cut(s) 110
SatI GCNGC 2 cut(s) 145, 148
Sau3AI GATC 3 cut(s) 21, 397, 472
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 2 cut(s) 173, 269
SduI GDGCHC 3 cut(s) 179, 232, 274
SetI ASST 7 cut(s) 60, 81, 191, 232, 546, 566, 579
SfaNI GCATC 1 cut(s) 147
SfcI CTRYAG 2 cut(s) 34, 145
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 565
SmoI CTYRAG 1 cut(s) 565
Sse9I AATT 6 cut(s) 72, 253, 334, 418, 428, 493
SsiI CCGC 1 cut(s) 42
SstI GAGCTC 1 cut(s) 232
StyD4I CCNGG 2 cut(s) 171, 267
TaaI ACNGT 2 cut(s) 38, 368
TaiI ACGT 2 cut(s) 60, 546
TaqI TCGA 1 cut(s) 445
TasI AATT 6 cut(s) 72, 253, 334, 418, 428, 493
TfiI GAWTC 1 cut(s) 451
TscAI CASTG 1 cut(s) 117
TseI GCWGC 2 cut(s) 144, 147
TspDTI ATGAA 7 cut(s) 17, 299, 360, 399, 417, 474, 492
TspRI CASTG 1 cut(s) 117
XapI RAATTY 3 cut(s) 72, 334, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.