Rw7G035950

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007557
Physical Location & Seq
Forward (+)
54032170 .. 54035245
3076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035950.1

Sequence Viewer

Length: 567 bp
ATGTCGAAGAGGTTTGGTAATCTGTTGATCGGCAAGCCCTACAGTTTCACTGTCGGCGCCAATGTCATCCGCCGTAGAATTTATCTGTTCCGCGAAGGGGAGAGGAGCATGATACTTCCGGTGAGCATCCGCTGCAACACTTGTGCCAATCGCATGCCCCAAGGCACCACTTTGAACTATGTAAGACGACGCCCCCTCTCCAAGACTTCTTTGGGAGCTCCAATAGTTACATTTTTCTTCCATTGTACCAAGTGCCGGGCACTCATCCGGATGAAGGAAGACCCAGAGAATTCAGGCTATGTTGTTGAGGATGGTGCTACAGAAGTTCTTGAACATGAGAAATCAGAACACCTTGGAAAGATTCGTGATGAAGATGGTTTGGATCCTGAAACATCTGACTCTAATTTGAAGAAATCTGGGCATCATCGAAAGATTCATGATGAAGCTGGCTTAGATTATGAAACATCCGACTCTGATTTGAAGAGACAAAAGCTTATGATTGGGCTTCCTAGCAACCCAACAGAGTCGTTCACTCTTTCGGATGGCTCAAGCACAGAGGTATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

188

Amino Acids

21.2

Weight (kDa)

8.26

Isoelectric Point (pI)

48.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 37 - 133 1.8e-17 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 56, 164
AccII CGCG 1 cut(s) 93
AccIII TCCGGA 1 cut(s) 267
AciI CCGC 3 cut(s) 70, 91, 130
AclWI GGATC 2 cut(s) 377, 390
AcsI RAATTY 2 cut(s) 78, 289
AcyI GRCGYC 2 cut(s) 57, 190
AfaI GTAC 1 cut(s) 247
AfiI CCNNNNNNNGG 3 cut(s) 97, 255, 274
AgsI TTSAA 4 cut(s) 175, 332, 409, 481
AluBI AGCT 3 cut(s) 218, 446, 493
AluI AGCT 3 cut(s) 218, 446, 493
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 1 cut(s) 478
AlwI GGATC 2 cut(s) 377, 390
Aor13HI TCCGGA 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 132
ApoI RAATTY 2 cut(s) 78, 289
AspLEI GCGC 1 cut(s) 59
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 262
BamHI GGATCC 1 cut(s) 382
BanI GGYRCC 2 cut(s) 56, 164
BanII GRGCYC 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 119
BccI CCATC 3 cut(s) 305, 368, 536
BceAI ACGGC 1 cut(s) 57
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 1 cut(s) 478
BfaI CTAG 2 cut(s) 510, 565
BfmI CTRYAG 2 cut(s) 40, 318
BfoI RGCGCY 1 cut(s) 60
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
Bme1390I CCNGG 1 cut(s) 257
BmiI GGNNCC 3 cut(s) 58, 166, 384
BmrFI CCNGG 1 cut(s) 257
BmsI GCATC 2 cut(s) 135, 430
BpiI GAAGAC 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 532
BpuMI CCSGG 1 cut(s) 257
BsaHI GRCGYC 2 cut(s) 57, 190
BsaJI CCNNGG 2 cut(s) 160, 352
BsaWI WCCGGW 2 cut(s) 118, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 255, 274
BseAI TCCGGA 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 160, 352
BseGI GGATG 7 cut(s) 66, 126, 264, 276, 316, 464, 547
BseLI CCNNNNNNNGG 3 cut(s) 97, 255, 274
BseRI GAGGAG 1 cut(s) 118
BseSI GKGCMC 1 cut(s) 262
BseXI GCAGC 1 cut(s) 119
Bsh1236I CGCG 1 cut(s) 93
BshNI GGYRCC 2 cut(s) 56, 164
BsiHKAI GWGCWC 1 cut(s) 220
BsiSI CCGG 3 cut(s) 119, 256, 268
BslI CCNNNNNNNGG 3 cut(s) 97, 255, 274
BsmAI GTCTC 1 cut(s) 478
Bsp1286I GDGCHC 2 cut(s) 220, 262
Bsp13I TCCGGA 1 cut(s) 267
Bsp143I GATC 2 cut(s) 27, 382
BspACI CCGC 3 cut(s) 70, 91, 130
BspEI TCCGGA 1 cut(s) 267
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 3 cut(s) 58, 166, 384
BspPI GGATC 2 cut(s) 377, 390
BspT107I GGYRCC 2 cut(s) 56, 164
BssECI CCNNGG 2 cut(s) 160, 352
BssMI GATC 2 cut(s) 27, 382
BssNI GRCGYC 2 cut(s) 57, 190
BssT1I CCWWGG 2 cut(s) 160, 352
Bst4CI ACNGT 2 cut(s) 44, 52
Bst6I CTCTTC 2 cut(s) 2, 476
BstACI GRCGYC 2 cut(s) 57, 190
BstAPI GCANNNNNTGC 1 cut(s) 132
BstC8I GCNNGC 3 cut(s) 35, 155, 448
BstDEI CTNAG 1 cut(s) 451
BstF5I GGATG 7 cut(s) 66, 126, 264, 276, 316, 464, 547
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 2 cut(s) 30, 385
BstMAI GTCTC 1 cut(s) 478
BstMBI GATC 2 cut(s) 27, 382
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 255
BstSFI CTRYAG 2 cut(s) 40, 318
BstSLI GKGCMC 1 cut(s) 262
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 119
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BtsCI GGATG 7 cut(s) 66, 126, 264, 276, 316, 464, 547
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 3 cut(s) 35, 155, 448
CciI TCATGA 1 cut(s) 436
CfoI GCGC 1 cut(s) 59
CseI GACGC 1 cut(s) 198
Csp6I GTAC 1 cut(s) 246
CviAII CATG 4 cut(s) 109, 154, 335, 437
CviJI RGCY 8 cut(s) 37, 218, 297, 446, 450, 493, 505, 546
CviKI_1 RGCY 8 cut(s) 37, 218, 297, 446, 450, 493, 505, 546
CviQI GTAC 1 cut(s) 246
DdeI CTNAG 1 cut(s) 451
DinI GGCGCC 1 cut(s) 58
DpnI GATC 2 cut(s) 29, 384
DpnII GATC 2 cut(s) 27, 382
Eam1104I CTCTTC 2 cut(s) 2, 476
EarI CTCTTC 2 cut(s) 2, 476
EciI GGCGGA 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 218
Eco130I CCWWGG 2 cut(s) 160, 352
Eco24I GRGCYC 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 218
EcoRI GAATTC 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 160, 352
EcoT38I GRGCYC 1 cut(s) 220
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
ErhI CCWWGG 2 cut(s) 160, 352
FaeI CATG 4 cut(s) 112, 157, 338, 440
FaiI YATR 9 cut(s) 110, 155, 180, 300, 336, 438, 459, 497, 562
FatI CATG 4 cut(s) 108, 153, 334, 436
Fnu4HI GCNGC 1 cut(s) 133
FokI GGATG 7 cut(s) 53, 113, 251, 283, 323, 451, 554
FriOI GRGCYC 1 cut(s) 220
Fsp4HI GCNGC 1 cut(s) 133
FspBI CTAG 2 cut(s) 510, 565
GlaI GCGC 1 cut(s) 58
GluI GCNGC 1 cut(s) 133
HaeII RGCGCY 1 cut(s) 60
HapII CCGG 3 cut(s) 119, 256, 268
HgaI GACGC 1 cut(s) 198
HhaI GCGC 1 cut(s) 59
Hin1I GRCGYC 2 cut(s) 57, 190
Hin1II CATG 4 cut(s) 112, 157, 338, 440
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 5 cut(s) 361, 398, 433, 470, 524
HpaII CCGG 3 cut(s) 119, 256, 268
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 1 cut(s) 531
Hpy188I TCNGA 5 cut(s) 346, 397, 469, 475, 541
Hpy188III TCNNGA 5 cut(s) 268, 329, 365, 386, 437
Hpy8I GTNNAC 1 cut(s) 531
Hpy99I CGWCG 1 cut(s) 192
HpyAV CCTTC 2 cut(s) 89, 268
HpyCH4III ACNGT 2 cut(s) 44, 52
HpyCH4V TGCA 1 cut(s) 135
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 1 cut(s) 451
Hsp92I GRCGYC 2 cut(s) 57, 190
Hsp92II CATG 4 cut(s) 112, 157, 338, 440
HspAI GCGC 1 cut(s) 57
KasI GGCGCC 1 cut(s) 56
Kpn2I TCCGGA 1 cut(s) 267
Kzo9I GATC 2 cut(s) 27, 382
LmnI GCTCC 3 cut(s) 105, 215, 223
LpnPI CCDG 8 cut(s) 132, 269, 279, 281, 297, 399, 402, 432
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 2 cut(s) 135, 430
MaeI CTAG 2 cut(s) 510, 565
MaeIII GTNAC 1 cut(s) 226
MalI GATC 2 cut(s) 29, 384
MboI GATC 2 cut(s) 27, 382
MboII GAAGA 6 cut(s) 19, 229, 290, 383, 421, 493
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 220, 262
MluCI AATT 3 cut(s) 78, 289, 403
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 3 cut(s) 392, 464, 533
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 5 cut(s) 3, 96, 206, 301, 550
MroI TCCGGA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 3 cut(s) 119, 256, 268
MspR9I CCNGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 132
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 1 cut(s) 257
NdeII GATC 2 cut(s) 27, 382
NlaIII CATG 4 cut(s) 112, 157, 338, 440
NlaIV GGNNCC 3 cut(s) 58, 166, 384
NspI RCATGY 1 cut(s) 157
PaeI GCATGC 1 cut(s) 157
PagI TCATGA 1 cut(s) 436
PfeI GAWTC 2 cut(s) 361, 433
PkrI GCNGC 1 cut(s) 134
PleI GAGTC 3 cut(s) 392, 464, 532
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 3 cut(s) 392, 464, 532
Psp124BI GAGCTC 1 cut(s) 220
PspN4I GGNNCC 3 cut(s) 58, 166, 384
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 1 cut(s) 247
RsaNI GTAC 1 cut(s) 246
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 1 cut(s) 220
SatI GCNGC 1 cut(s) 133
Sau3AI GATC 2 cut(s) 27, 382
SchI GAGTC 3 cut(s) 392, 464, 533
ScrFI CCNGG 1 cut(s) 257
SduI GDGCHC 2 cut(s) 220, 262
SetI ASST 6 cut(s) 14, 220, 354, 448, 495, 561
SfaNI GCATC 2 cut(s) 135, 430
SfcI CTRYAG 2 cut(s) 40, 318
SfoI GGCGCC 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 547
SmoI CTYRAG 1 cut(s) 547
SphI GCATGC 1 cut(s) 157
Sse9I AATT 3 cut(s) 78, 289, 403
SsiI CCGC 3 cut(s) 70, 91, 130
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 2 cut(s) 510, 565
SstI GAGCTC 1 cut(s) 220
StyD4I CCNGG 1 cut(s) 255
StyI CCWWGG 2 cut(s) 160, 352
TaaI ACNGT 2 cut(s) 44, 52
TaqI TCGA 2 cut(s) 5, 427
TasI AATT 3 cut(s) 78, 289, 403
TfiI GAWTC 2 cut(s) 361, 433
TscAI CASTG 1 cut(s) 55
TseI GCWGC 1 cut(s) 132
TspDTI ATGAA 5 cut(s) 287, 384, 425, 456, 474
TspRI CASTG 1 cut(s) 55
XapI RAATTY 2 cut(s) 78, 289
XceI RCATGY 1 cut(s) 157
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XspI CTAG 2 cut(s) 510, 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.