Rw7G035940

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007557
Physical Location & Seq
Forward (+)
53962062 .. 53965111
3050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035940.1

Sequence Viewer

Length: 552 bp
ATGTCGAAGAGGTTTGGTAATCTGTTGATCGGCAAGAACTACGGTCTCCCTGTCCGCCGTAGAATTTATCTGTTCCGCGAAGGGGAGAGGAGCATGATACTTCCGGTGAGCATCCGCTGCAACACTTGTGCCAATCGCATGCCCCAAGGCACCACTTTGAACTATGTAAGAAGACGCCCCCTCTCCAAGACTTCTTTGGGAGCTCCAATAGTTACATTTTTCTTCAATTGTACCAAGTGCCGGGCACTCATCCGGATGAAGGAAGACCCGGAGAATTCAGGATATGTTGTTGAGGATGGTGCTACAGAAGTTCTTGAACATGAGAAATCAGAACGCCTTGGAAAGATTCGTGATGAAGATGGTTTGGATCCTGAAACATCTGACTCTAATTTGAAGAAATCTGGGCATCATCGAAGGATTCATGATGAAGCTGGCTTAGATTATGAAACATCCGACTCTGATTTGAAGAGACAAAAGCTTATGATTGGGCTTCCTAGCAACCCAACAGAGTCGTTCACTCTTTCGGATAGCTCAAGCACAGAGGTATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

183

Amino Acids

20.74

Weight (kDa)

8.6

Isoelectric Point (pI)

58.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 32 - 125 2.3e-17 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 149
AccII CGCG 1 cut(s) 78
AccIII TCCGGA 1 cut(s) 252
AciI CCGC 3 cut(s) 55, 76, 115
AclWI GGATC 2 cut(s) 362, 375
AcsI RAATTY 2 cut(s) 63, 274
AcyI GRCGYC 1 cut(s) 175
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 3 cut(s) 82, 240, 259
AgsI TTSAA 5 cut(s) 160, 226, 317, 394, 466
AluBI AGCT 4 cut(s) 203, 431, 478, 531
AluI AGCT 4 cut(s) 203, 431, 478, 531
Alw21I GWGCWC 1 cut(s) 205
Alw26I GTCTC 2 cut(s) 50, 463
AlwI GGATC 2 cut(s) 362, 375
Aor13HI TCCGGA 1 cut(s) 252
ApeKI GCWGC 1 cut(s) 117
ApoI RAATTY 2 cut(s) 63, 274
AsuC2I CCSGG 2 cut(s) 242, 269
AsuHPI GGTGA 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 247
BamHI GGATCC 1 cut(s) 367
BanI GGYRCC 1 cut(s) 149
BanII GRGCYC 1 cut(s) 205
BbsI GAAGAC 2 cut(s) 178, 270
Bbv12I GWGCWC 1 cut(s) 205
BbvI GCAGC 1 cut(s) 104
BccI CCATC 2 cut(s) 290, 353
BceAI ACGGC 1 cut(s) 42
BcnI CCSGG 2 cut(s) 242, 269
BcoDI GTCTC 2 cut(s) 50, 463
BfaI CTAG 2 cut(s) 495, 550
BfmI CTRYAG 1 cut(s) 303
BisI GCNGC 1 cut(s) 118
BlsI GCNGC 1 cut(s) 119
Bme1390I CCNGG 2 cut(s) 242, 269
BmiI GGNNCC 2 cut(s) 151, 369
BmrFI CCNGG 2 cut(s) 242, 269
BmsI GCATC 2 cut(s) 120, 415
BpiI GAAGAC 2 cut(s) 178, 270
BpuEI CTTGAG 1 cut(s) 517
BpuMI CCSGG 2 cut(s) 242, 269
BsaHI GRCGYC 1 cut(s) 175
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 145, 337
BsaWI WCCGGW 2 cut(s) 103, 252
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 240, 259
BseAI TCCGGA 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 145, 337
BseGI GGATG 5 cut(s) 111, 249, 261, 301, 449
BseLI CCNNNNNNNGG 3 cut(s) 82, 240, 259
BseRI GAGGAG 1 cut(s) 103
BseSI GKGCMC 1 cut(s) 247
BseXI GCAGC 1 cut(s) 104
Bsh1236I CGCG 1 cut(s) 78
BshNI GGYRCC 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 205
BsiSI CCGG 4 cut(s) 104, 241, 253, 269
BslI CCNNNNNNNGG 3 cut(s) 82, 240, 259
BsmAI GTCTC 2 cut(s) 50, 463
Bso31I GGTCTC 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 205, 247
Bsp13I TCCGGA 1 cut(s) 252
Bsp143I GATC 2 cut(s) 27, 367
BspACI CCGC 3 cut(s) 55, 76, 115
BspEI TCCGGA 1 cut(s) 252
BspFNI CGCG 1 cut(s) 78
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 2 cut(s) 151, 369
BspPI GGATC 2 cut(s) 362, 375
BspT107I GGYRCC 1 cut(s) 149
BspTNI GGTCTC 1 cut(s) 50
BssECI CCNNGG 2 cut(s) 145, 337
BssMI GATC 2 cut(s) 27, 367
BssNI GRCGYC 1 cut(s) 175
BssT1I CCWWGG 2 cut(s) 145, 337
Bst4CI ACNGT 1 cut(s) 44
Bst6I CTCTTC 2 cut(s) 2, 461
BstACI GRCGYC 1 cut(s) 175
BstAPI GCANNNNNTGC 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 140, 433
BstDEI CTNAG 1 cut(s) 436
BstF5I GGATG 5 cut(s) 111, 249, 261, 301, 449
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 2 cut(s) 30, 370
BstMAI GTCTC 2 cut(s) 50, 463
BstMBI GATC 2 cut(s) 27, 367
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 2 cut(s) 240, 267
BstSFI CTRYAG 1 cut(s) 303
BstSLI GKGCMC 1 cut(s) 247
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 1 cut(s) 104
BstV2I GAAGAC 2 cut(s) 178, 270
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BtsCI GGATG 5 cut(s) 111, 249, 261, 301, 449
Cac8I GCNNGC 2 cut(s) 140, 433
CciI TCATGA 1 cut(s) 421
CseI GACGC 1 cut(s) 183
Csp6I GTAC 1 cut(s) 231
CviAII CATG 4 cut(s) 94, 139, 320, 422
CviJI RGCY 6 cut(s) 203, 431, 435, 478, 490, 531
CviKI_1 RGCY 6 cut(s) 203, 431, 435, 478, 490, 531
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 1 cut(s) 436
DpnI GATC 2 cut(s) 29, 369
DpnII GATC 2 cut(s) 27, 367
Eam1104I CTCTTC 2 cut(s) 2, 461
EarI CTCTTC 2 cut(s) 2, 461
EciI GGCGGA 1 cut(s) 44
Ecl136II GAGCTC 1 cut(s) 203
Eco130I CCWWGG 2 cut(s) 145, 337
Eco24I GRGCYC 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 50
Eco53kI GAGCTC 1 cut(s) 203
EcoICRI GAGCTC 1 cut(s) 203
EcoRI GAATTC 1 cut(s) 274
EcoT14I CCWWGG 2 cut(s) 145, 337
EcoT38I GRGCYC 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 145, 337
FaeI CATG 4 cut(s) 97, 142, 323, 425
FaiI YATR 9 cut(s) 95, 140, 165, 285, 321, 423, 444, 482, 547
FatI CATG 4 cut(s) 93, 138, 319, 421
Fnu4HI GCNGC 1 cut(s) 118
FokI GGATG 5 cut(s) 98, 236, 268, 308, 436
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 1 cut(s) 118
FspBI CTAG 2 cut(s) 495, 550
GluI GCNGC 1 cut(s) 118
HapII CCGG 4 cut(s) 104, 241, 253, 269
HgaI GACGC 1 cut(s) 183
Hin1I GRCGYC 1 cut(s) 175
Hin1II CATG 4 cut(s) 97, 142, 323, 425
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 5 cut(s) 346, 383, 418, 455, 509
HpaII CCGG 4 cut(s) 104, 241, 253, 269
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 516
Hpy188I TCNGA 5 cut(s) 331, 382, 454, 460, 526
Hpy188III TCNNGA 6 cut(s) 253, 279, 314, 350, 371, 422
Hpy8I GTNNAC 1 cut(s) 516
HpyAV CCTTC 3 cut(s) 74, 253, 408
HpyCH4III ACNGT 1 cut(s) 44
HpyCH4V TGCA 1 cut(s) 120
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 1 cut(s) 436
Hsp92I GRCGYC 1 cut(s) 175
Hsp92II CATG 4 cut(s) 97, 142, 323, 425
Kpn2I TCCGGA 1 cut(s) 252
Kzo9I GATC 2 cut(s) 27, 367
LmnI GCTCC 3 cut(s) 90, 200, 208
LpnPI CCDG 9 cut(s) 63, 117, 254, 264, 266, 282, 384, 387, 417
Lsp1109I GCAGC 1 cut(s) 104
LweI GCATC 2 cut(s) 120, 415
MaeI CTAG 2 cut(s) 495, 550
MaeIII GTNAC 1 cut(s) 211
MalI GATC 2 cut(s) 29, 369
MboI GATC 2 cut(s) 27, 367
MboII GAAGA 7 cut(s) 19, 183, 214, 275, 368, 406, 478
MfeI CAATTG 1 cut(s) 226
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 2 cut(s) 205, 247
MluCI AATT 4 cut(s) 63, 226, 274, 388
MlyI GAGTC 3 cut(s) 377, 449, 518
MmeI TCCRAC 1 cut(s) 477
MnlI CCTC 5 cut(s) 3, 81, 191, 286, 535
MroI TCCGGA 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 4 cut(s) 104, 241, 253, 269
MspR9I CCNGG 2 cut(s) 242, 269
MunI CAATTG 1 cut(s) 226
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 117
NciI CCSGG 2 cut(s) 242, 269
NdeII GATC 2 cut(s) 27, 367
NlaIII CATG 4 cut(s) 97, 142, 323, 425
NlaIV GGNNCC 2 cut(s) 151, 369
NspI RCATGY 1 cut(s) 142
PaeI GCATGC 1 cut(s) 142
PagI TCATGA 1 cut(s) 421
PfeI GAWTC 2 cut(s) 346, 418
PkrI GCNGC 1 cut(s) 119
PleI GAGTC 3 cut(s) 377, 449, 517
PpsI GAGTC 3 cut(s) 377, 449, 517
Psp124BI GAGCTC 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 151, 369
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
RseI CAYNNNNRTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 205
SatI GCNGC 1 cut(s) 118
Sau3AI GATC 2 cut(s) 27, 367
SchI GAGTC 3 cut(s) 377, 449, 518
ScrFI CCNGG 2 cut(s) 242, 269
SduI GDGCHC 2 cut(s) 205, 247
SetI ASST 6 cut(s) 14, 205, 433, 480, 533, 546
SfaNI GCATC 2 cut(s) 120, 415
SfcI CTRYAG 1 cut(s) 303
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
SphI GCATGC 1 cut(s) 142
Sse9I AATT 4 cut(s) 63, 226, 274, 388
SsiI CCGC 3 cut(s) 55, 76, 115
SspMI CTAG 2 cut(s) 495, 550
SstI GAGCTC 1 cut(s) 205
StyD4I CCNGG 2 cut(s) 240, 267
StyI CCWWGG 2 cut(s) 145, 337
TaaI ACNGT 1 cut(s) 44
TaqI TCGA 2 cut(s) 5, 412
TasI AATT 4 cut(s) 63, 226, 274, 388
TfiI GAWTC 2 cut(s) 346, 418
TseI GCWGC 1 cut(s) 117
TspDTI ATGAA 5 cut(s) 272, 369, 410, 441, 459
XapI RAATTY 2 cut(s) 63, 274
XceI RCATGY 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XspI CTAG 2 cut(s) 495, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.