Rh7AG436200

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0007557
Physical Location & Seq
Forward (+)
58755185 .. 58760848
5664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG436200.1

Sequence Viewer

Length: 555 bp
ATGTCGAAGAGGTTTGGTAATTTGTTGATTGGCAAGCCCTACAGTCTCACTGTCGGCTTCAATGTCCTCCGCTGTAGAATTTATCTGTTCCGCGAAGGAGAGAGGAGGTGGTTACCGCTGAAGACCATGATACTTCCGGTGAGCATCCGCTGGAACACTTGTGCCAATCGCATGCCCCAAGGCACCACTTTGAATTATGTAAGACGACGCCCCCTTTCCAAGACTTCTTTGGGAGCTCCAATAGTTACATTTTTCTTCAATTGTACCAAGTGTCGGGCACTCATCCAGATAAAGGAAGACCCAGAGAATTCAGGCTATGTTGTTGAGGATGGTGCTACAGAAGTTCTTGAACATGAGAAATCAGAACACCTTGGAAAGATTCGTGATGAAGATGGTTTGGATCCTGAAACATCTGACTCTAATTTGAAGACTCCGGAAGATCAGCAGTCTAGCCCAGTTGCTTCCCCTCCAGTCGCTTCCTCAGATTCTGGATCCCTCTCTCAGGTCAGCCAGAAAGGGAGAGCAATCAGATTTTTATGCTTTTTGAGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

184

Amino Acids

20.78

Weight (kDa)

9.34

Isoelectric Point (pI)

65.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 43 - 143 1.7e-14 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 182
AccII CGCG 1 cut(s) 93
AccIII TCCGGA 1 cut(s) 433
AciI CCGC 4 cut(s) 70, 91, 116, 148
AclWI GGATC 4 cut(s) 395, 408, 486, 499
AcsI RAATTY 2 cut(s) 78, 307
AcuI CTGAAG 1 cut(s) 140
AcyI GRCGYC 1 cut(s) 208
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 3 cut(s) 273, 292, 502
AgsI TTSAA 5 cut(s) 61, 193, 259, 350, 427
AluBI AGCT 1 cut(s) 236
AluI AGCT 1 cut(s) 236
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 1 cut(s) 50
AlwI GGATC 4 cut(s) 395, 408, 486, 499
AlwNI CAGNNNCTG 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 433
ApoI RAATTY 2 cut(s) 78, 307
AsuHPI GGTGA 1 cut(s) 151
BaeGI GKGCMC 1 cut(s) 280
BamHI GGATCC 2 cut(s) 400, 491
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 238
BbsI GAAGAC 3 cut(s) 128, 303, 434
Bbv12I GWGCWC 1 cut(s) 238
BccI CCATC 2 cut(s) 323, 386
BcoDI GTCTC 1 cut(s) 50
BfaI CTAG 1 cut(s) 450
BfmI CTRYAG 3 cut(s) 40, 73, 336
BmiI GGNNCC 3 cut(s) 184, 402, 493
BmrI ACTGGG 1 cut(s) 449
BmsI GCATC 1 cut(s) 153
BmuI ACTGGG 1 cut(s) 449
BpiI GAAGAC 3 cut(s) 128, 303, 434
BpmI CTGGAG 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 208
BsaJI CCNNGG 2 cut(s) 178, 370
BsaWI WCCGGW 2 cut(s) 136, 433
Bsc4I CCNNNNNNNGG 3 cut(s) 273, 292, 502
Bse1I ACTGG 2 cut(s) 455, 470
BseAI TCCGGA 1 cut(s) 433
BseDI CCNNGG 2 cut(s) 178, 370
BseGI GGATG 3 cut(s) 144, 282, 334
BseLI CCNNNNNNNGG 3 cut(s) 273, 292, 502
BseMII CTCAG 2 cut(s) 495, 515
BseNI ACTGG 2 cut(s) 455, 470
BseRI GAGGAG 1 cut(s) 118
BseSI GKGCMC 1 cut(s) 280
Bsh1236I CGCG 1 cut(s) 93
BshNI GGYRCC 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 238
BsiSI CCGG 2 cut(s) 137, 434
BslI CCNNNNNNNGG 3 cut(s) 273, 292, 502
BsmAI GTCTC 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 238, 280
Bsp13I TCCGGA 1 cut(s) 433
Bsp143I GATC 3 cut(s) 400, 439, 491
BspACI CCGC 4 cut(s) 70, 91, 116, 148
BspCNI CTCAG 2 cut(s) 494, 514
BspEI TCCGGA 1 cut(s) 433
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 184, 402, 493
BspPI GGATC 4 cut(s) 395, 408, 486, 499
BspT107I GGYRCC 1 cut(s) 182
BsrI ACTGG 2 cut(s) 455, 470
BssECI CCNNGG 2 cut(s) 178, 370
BssMI GATC 3 cut(s) 400, 439, 491
BssNI GRCGYC 1 cut(s) 208
BssT1I CCWWGG 2 cut(s) 178, 370
Bst4CI ACNGT 2 cut(s) 44, 52
Bst6I CTCTTC 1 cut(s) 2
BstACI GRCGYC 1 cut(s) 208
BstC8I GCNNGC 2 cut(s) 35, 173
BstDEI CTNAG 2 cut(s) 481, 501
BstEII GGTNACC 1 cut(s) 111
BstENI CCTNNNNNAGG 1 cut(s) 500
BstF5I GGATG 3 cut(s) 144, 282, 334
BstFNI CGCG 1 cut(s) 93
BstKTI GATC 3 cut(s) 403, 442, 494
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 3 cut(s) 400, 439, 491
BstNSI RCATGY 1 cut(s) 175
BstPI GGTNACC 1 cut(s) 111
BstSFI CTRYAG 3 cut(s) 40, 73, 336
BstSLI GKGCMC 1 cut(s) 280
BstUI CGCG 1 cut(s) 93
BstV2I GAAGAC 3 cut(s) 128, 303, 434
BstX2I RGATCY 2 cut(s) 400, 491
BstYI RGATCY 2 cut(s) 400, 491
BtsCI GGATG 3 cut(s) 144, 282, 334
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 2 cut(s) 35, 173
CaiI CAGNNNCTG 1 cut(s) 488
CseI GACGC 1 cut(s) 216
Csp6I GTAC 1 cut(s) 264
CviAII CATG 3 cut(s) 127, 172, 353
CviJI RGCY 6 cut(s) 37, 57, 236, 315, 453, 510
CviKI_1 RGCY 6 cut(s) 37, 57, 236, 315, 453, 510
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 2 cut(s) 481, 501
DpnI GATC 3 cut(s) 402, 441, 493
DpnII GATC 3 cut(s) 400, 439, 491
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
Ecl136II GAGCTC 1 cut(s) 236
Eco130I CCWWGG 2 cut(s) 178, 370
Eco24I GRGCYC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 236
Eco57I CTGAAG 1 cut(s) 140
Eco91I GGTNACC 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 236
EcoNI CCTNNNNNAGG 1 cut(s) 500
EcoO65I GGTNACC 1 cut(s) 111
EcoRI GAATTC 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 178, 370
EcoT38I GRGCYC 1 cut(s) 238
ErhI CCWWGG 2 cut(s) 178, 370
FaeI CATG 3 cut(s) 130, 175, 356
FaiI YATR 6 cut(s) 128, 173, 198, 318, 354, 538
FatI CATG 3 cut(s) 126, 171, 352
FokI GGATG 3 cut(s) 131, 269, 341
FriOI GRGCYC 1 cut(s) 238
FspBI CTAG 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 453
HapII CCGG 2 cut(s) 137, 434
HgaI GACGC 1 cut(s) 216
Hin1I GRCGYC 1 cut(s) 208
Hin1II CATG 3 cut(s) 130, 175, 356
HinfI GANTC 4 cut(s) 379, 416, 430, 485
HpaII CCGG 2 cut(s) 137, 434
HphI GGTGA 1 cut(s) 151
Hpy188I TCNGA 4 cut(s) 364, 415, 484, 530
Hpy188III TCNNGA 6 cut(s) 286, 347, 383, 404, 434, 489
Hpy99I CGWCG 1 cut(s) 210
HpyAV CCTTC 1 cut(s) 89
HpyCH4III ACNGT 2 cut(s) 44, 52
HpyF3I CTNAG 2 cut(s) 481, 501
Hsp92I GRCGYC 1 cut(s) 208
Hsp92II CATG 3 cut(s) 130, 175, 356
Kpn2I TCCGGA 1 cut(s) 433
Kzo9I GATC 3 cut(s) 400, 439, 491
LmnI GCTCC 2 cut(s) 233, 241
LweI GCATC 1 cut(s) 153
MaeI CTAG 1 cut(s) 450
MaeIII GTNAC 2 cut(s) 111, 244
MalI GATC 3 cut(s) 402, 441, 493
MboI GATC 3 cut(s) 400, 439, 491
MboII GAAGA 7 cut(s) 19, 133, 247, 308, 401, 439, 449
MfeI CAATTG 1 cut(s) 259
MflI RGATCY 2 cut(s) 400, 491
MhlI GDGCHC 2 cut(s) 238, 280
MluCI AATT 6 cut(s) 19, 78, 193, 259, 307, 421
MlyI GAGTC 2 cut(s) 410, 424
MroI TCCGGA 1 cut(s) 433
MspA1I CMGCKG 3 cut(s) 72, 118, 150
MspI CCGG 2 cut(s) 137, 434
MunI CAATTG 1 cut(s) 259
MvnI CGCG 1 cut(s) 93
NdeII GATC 3 cut(s) 400, 439, 491
NlaIII CATG 3 cut(s) 130, 175, 356
NlaIV GGNNCC 3 cut(s) 184, 402, 493
NspI RCATGY 1 cut(s) 175
PaeI GCATGC 1 cut(s) 175
PfeI GAWTC 2 cut(s) 379, 485
PleI GAGTC 2 cut(s) 410, 424
PpsI GAGTC 2 cut(s) 410, 424
Psp124BI GAGCTC 1 cut(s) 238
PspEI GGTNACC 1 cut(s) 111
PspN4I GGNNCC 3 cut(s) 184, 402, 493
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 2 cut(s) 400, 491
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SacI GAGCTC 1 cut(s) 238
Sau3AI GATC 3 cut(s) 400, 439, 491
SchI GAGTC 2 cut(s) 410, 424
SduI GDGCHC 2 cut(s) 238, 280
SetI ASST 6 cut(s) 14, 110, 238, 372, 507, 554
SfaNI GCATC 1 cut(s) 153
SfcI CTRYAG 3 cut(s) 40, 73, 336
SphI GCATGC 1 cut(s) 175
Sse9I AATT 6 cut(s) 19, 78, 193, 259, 307, 421
SsiI CCGC 4 cut(s) 70, 91, 116, 148
SspMI CTAG 1 cut(s) 450
SstI GAGCTC 1 cut(s) 238
StyI CCWWGG 2 cut(s) 178, 370
TaaI ACNGT 2 cut(s) 44, 52
TaqI TCGA 1 cut(s) 5
TasI AATT 6 cut(s) 19, 78, 193, 259, 307, 421
TfiI GAWTC 2 cut(s) 379, 485
TscAI CASTG 1 cut(s) 55
TspDTI ATGAA 1 cut(s) 402
TspRI CASTG 1 cut(s) 55
XagI CCTNNNNNAGG 1 cut(s) 500
XapI RAATTY 2 cut(s) 78, 307
XceI RCATGY 1 cut(s) 175
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XspI CTAG 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.