Rh4BG223600

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007557
Physical Location & Seq
Reverse (-)
38653523 .. 38654826
1304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG223600.1

Sequence Viewer

Length: 390 bp
ATGTCGAAGAGGTTTGGGAATCTGTTGATCGGCAAGACCTACGGTCTCACTGTCCGCCGTAGAATTTATCTGTTCCGCGAAGGAGAGAGGAGGTGGTTACCGCTGAAGACCATGATACTTCCGGTGAGCATCCGCTGCAACACTTGTGCCAATCGCATGCCCCAAGGCACCACTTTGAACTATGTAAGACGACGCCCCCTCTCCAAGACTTCTCTGGGAGCTCCAATAGTTACATTTTTCTTCAATTGTACCAAGTGCCGGGCACTCATCCAGATGAAGGAAGACCCAGAGAATTCAGGCCATGTTGTTGAGGATGGTGCTACAGAAGTTCTTGAACATGAGAAATCAGAACACCTTGGAAAGATTCATGATGAAGATGGTTTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

129

Amino Acids

14.82

Weight (kDa)

9.54

Isoelectric Point (pI)

60.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 38 - 117 2.5e-15 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccII CGCG 1 cut(s) 78
AciI CCGC 4 cut(s) 55, 76, 101, 133
AcsI RAATTY 2 cut(s) 63, 292
AcuI CTGAAG 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 1 cut(s) 250
AfiI CCNNNNNNNGG 2 cut(s) 258, 277
AgsI TTSAA 3 cut(s) 178, 244, 335
AhdI GACNNNNNGTC 1 cut(s) 42
AluBI AGCT 1 cut(s) 221
AluI AGCT 1 cut(s) 221
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 50
AoxI GGCC 1 cut(s) 298
ApeKI GCWGC 1 cut(s) 135
ApoI RAATTY 2 cut(s) 63, 292
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 223
BbsI GAAGAC 2 cut(s) 113, 288
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 1 cut(s) 122
BccI CCATC 2 cut(s) 308, 371
BceAI ACGGC 1 cut(s) 42
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 321
BisI GCNGC 1 cut(s) 136
BlsI GCNGC 1 cut(s) 137
Bme1390I CCNGG 1 cut(s) 260
BmeRI GACNNNNNGTC 1 cut(s) 42
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 1 cut(s) 260
BmsI GCATC 1 cut(s) 138
BpiI GAAGAC 2 cut(s) 113, 288
BpuMI CCSGG 1 cut(s) 260
BsaHI GRCGYC 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 163, 355
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 277
BseDI CCNNGG 2 cut(s) 163, 355
BseGI GGATG 3 cut(s) 129, 267, 319
BseLI CCNNNNNNNGG 2 cut(s) 258, 277
BseRI GAGGAG 1 cut(s) 103
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 1 cut(s) 122
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 300
BshNI GGYRCC 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 2 cut(s) 122, 259
BslI CCNNNNNNNGG 2 cut(s) 258, 277
BsmAI GTCTC 1 cut(s) 50
BsnI GGCC 1 cut(s) 300
Bso31I GGTCTC 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 223, 265
Bsp143I GATC 1 cut(s) 27
BspACI CCGC 4 cut(s) 55, 76, 101, 133
BspANI GGCC 1 cut(s) 300
BspFNI CGCG 1 cut(s) 78
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 169
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 50
BssECI CCNNGG 2 cut(s) 163, 355
BssMI GATC 1 cut(s) 27
BssNI GRCGYC 1 cut(s) 193
BssT1I CCWWGG 2 cut(s) 163, 355
Bst4CI ACNGT 2 cut(s) 44, 52
Bst6I CTCTTC 1 cut(s) 2
BstACI GRCGYC 1 cut(s) 193
BstAPI GCANNNNNTGC 1 cut(s) 135
BstC8I GCNNGC 1 cut(s) 158
BstEII GGTNACC 1 cut(s) 96
BstF5I GGATG 3 cut(s) 129, 267, 319
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 1 cut(s) 30
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 1 cut(s) 135
BstNSI RCATGY 1 cut(s) 160
BstPI GGTNACC 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 258
BstSFI CTRYAG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 265
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 1 cut(s) 122
BstV2I GAAGAC 2 cut(s) 113, 288
BsuRI GGCC 1 cut(s) 300
BtsCI GGATG 3 cut(s) 129, 267, 319
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 158
CciI TCATGA 1 cut(s) 367
CseI GACGC 1 cut(s) 201
Csp6I GTAC 1 cut(s) 249
CviAII CATG 5 cut(s) 112, 157, 302, 338, 368
CviJI RGCY 2 cut(s) 221, 300
CviKI_1 RGCY 2 cut(s) 221, 300
CviQI GTAC 1 cut(s) 249
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
DriI GACNNNNNGTC 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 2
Eam1105I GACNNNNNGTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 2
EciI GGCGGA 1 cut(s) 44
Ecl136II GAGCTC 1 cut(s) 221
Eco130I CCWWGG 2 cut(s) 163, 355
Eco24I GRGCYC 1 cut(s) 223
Eco31I GGTCTC 1 cut(s) 50
Eco53kI GAGCTC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 125
Eco91I GGTNACC 1 cut(s) 96
EcoICRI GAGCTC 1 cut(s) 221
EcoO65I GGTNACC 1 cut(s) 96
EcoRI GAATTC 1 cut(s) 292
EcoT14I CCWWGG 2 cut(s) 163, 355
EcoT38I GRGCYC 1 cut(s) 223
ErhI CCWWGG 2 cut(s) 163, 355
FaeI CATG 5 cut(s) 115, 160, 305, 341, 371
FaiI YATR 6 cut(s) 113, 158, 183, 303, 339, 369
FatI CATG 5 cut(s) 111, 156, 301, 337, 367
Fnu4HI GCNGC 1 cut(s) 136
FokI GGATG 3 cut(s) 116, 254, 326
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 1 cut(s) 136
GluI GCNGC 1 cut(s) 136
HaeIII GGCC 1 cut(s) 300
HapII CCGG 2 cut(s) 122, 259
HgaI GACGC 1 cut(s) 201
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 5 cut(s) 115, 160, 305, 341, 371
HinfI GANTC 2 cut(s) 19, 364
HpaII CCGG 2 cut(s) 122, 259
HphI GGTGA 1 cut(s) 136
Hpy188I TCNGA 1 cut(s) 349
Hpy188III TCNNGA 3 cut(s) 271, 332, 368
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 2 cut(s) 74, 271
HpyCH4III ACNGT 2 cut(s) 44, 52
HpyCH4V TGCA 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 5 cut(s) 115, 160, 305, 341, 371
Kzo9I GATC 1 cut(s) 27
LmnI GCTCC 2 cut(s) 218, 226
LpnPI CCDG 6 cut(s) 135, 200, 272, 282, 284, 300
Lsp1109I GCAGC 1 cut(s) 122
LweI GCATC 1 cut(s) 138
MaeIII GTNAC 2 cut(s) 96, 229
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 5 cut(s) 19, 118, 232, 293, 386
MfeI CAATTG 1 cut(s) 244
MhlI GDGCHC 2 cut(s) 223, 265
MluCI AATT 3 cut(s) 63, 244, 292
MnlI CCTC 5 cut(s) 3, 81, 84, 209, 304
MslI CAYNNNNRTG 1 cut(s) 272
MspA1I CMGCKG 2 cut(s) 103, 135
MspI CCGG 2 cut(s) 122, 259
MspR9I CCNGG 1 cut(s) 260
MunI CAATTG 1 cut(s) 244
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 135
NciI CCSGG 1 cut(s) 260
NdeII GATC 1 cut(s) 27
NlaIII CATG 5 cut(s) 115, 160, 305, 341, 371
NlaIV GGNNCC 1 cut(s) 169
NspI RCATGY 1 cut(s) 160
PaeI GCATGC 1 cut(s) 160
PagI TCATGA 1 cut(s) 367
PfeI GAWTC 2 cut(s) 19, 364
PkrI GCNGC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 223
PspEI GGTNACC 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 169
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
RseI CAYNNNNRTG 1 cut(s) 272
SacI GAGCTC 1 cut(s) 223
SatI GCNGC 1 cut(s) 136
Sau3AI GATC 1 cut(s) 27
ScrFI CCNGG 1 cut(s) 260
SduI GDGCHC 2 cut(s) 223, 265
SetI ASST 5 cut(s) 14, 41, 95, 223, 357
SfaNI GCATC 1 cut(s) 138
SfcI CTRYAG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 272
SphI GCATGC 1 cut(s) 160
Sse9I AATT 3 cut(s) 63, 244, 292
SsiI CCGC 4 cut(s) 55, 76, 101, 133
SstI GAGCTC 1 cut(s) 223
StyD4I CCNGG 1 cut(s) 258
StyI CCWWGG 2 cut(s) 163, 355
TaaI ACNGT 2 cut(s) 44, 52
TaqI TCGA 1 cut(s) 5
TasI AATT 3 cut(s) 63, 244, 292
TfiI GAWTC 2 cut(s) 19, 364
TscAI CASTG 1 cut(s) 55
TseI GCWGC 1 cut(s) 135
TspDTI ATGAA 3 cut(s) 290, 356, 387
TspRI CASTG 1 cut(s) 55
XapI RAATTY 2 cut(s) 63, 292
XceI RCATGY 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.