RLG00000006362

function. Its presence in a non-photosynthetic plant (Epifagus virginiana) and experiments in tobacco indicate that it has an essential function which is probably not related to photosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004531
Physical Location & Seq
Reverse (-)
6507952 .. 6509465
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006362

Sequence Viewer

Length: 537 bp
ATGGAAGCCCCCCCGGAACAATTAGGAGATTCTCTAGAAGAAATACAGGGTTCCGCTTCTGGCGACAACATGCTATGGGGTGGTGGTCTCGCTTATGGTGTCAAATCAATACGTTCTAAGAAGAAATATTTGAATATAAATCTCATCGATTTCATAAACATCTCGTCCGGTGGCTTTCCGGCGTCGCCTCGACGTCGTGGGGATGTTGTCGGATGGGTAGCATATGGTTTAGACCTCTGGAATCTCTTTGTCTATGGTGTGGCAGTTGTAGCTGGGCCTATTCGGATTGAGATGGCTTTGGGGCTGGAGGTTTTGTGCACCGGTTTCAAGGAAGGTGACGACGGAGGAGCAGTTTCGCTCCAGACAACGGCGACCTTTACGGCCACCATTGATGGGGTTAGCGGTTTCGATGCAGAGACCCTTACCTGTTTCGGCTTGTCCTTTGTCTGGGTGGTGGTGCAAGATGGTTGGGCGGCTGAACGGGGCGGTGGCGTCGGTGAAGGGTCACAGCCGCTGCAGGTGGTGCGTGGAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA DUF825

Protein Analysis

179

Amino Acids

18.66

Weight (kDa)

4.45

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf2 PF05695 6 - 56 3.8e-20 Ycf2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 508
AatII GACGTC 1 cut(s) 196
Acc36I ACCTGC 1 cut(s) 508
AciI CCGC 5 cut(s) 54, 402, 473, 486, 512
AcoI YGGCCR 1 cut(s) 381
AcyI GRCGYC 3 cut(s) 182, 193, 492
AfiI CCNNNNNNNGG 3 cut(s) 367, 393, 447
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 2 cut(s) 133, 328
AluBI AGCT 1 cut(s) 272
AluI AGCT 1 cut(s) 272
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 2 cut(s) 92, 410
Alw44I GTGCAC 1 cut(s) 316
AlwNI CAGNNNCTG 1 cut(s) 514
AoxI GGCC 2 cut(s) 275, 381
ApaLI GTGCAC 1 cut(s) 316
ApeKI GCWGC 1 cut(s) 514
AsiGI ACCGGT 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 275
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 2 cut(s) 347, 509
BaeGI GKGCMC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 501
BccI CCATC 4 cut(s) 207, 286, 386, 458
BceAI ACGGC 2 cut(s) 384, 396
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 2 cut(s) 92, 410
BfaI CTAG 1 cut(s) 35
BfmI CTRYAG 1 cut(s) 515
BfuAI ACCTGC 1 cut(s) 508
BisI GCNGC 3 cut(s) 474, 512, 515
BlsI GCNGC 3 cut(s) 475, 513, 516
Bme1390I CCNGG 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 14
BmsI GCATC 1 cut(s) 400
BpmI CTGGAG 2 cut(s) 326, 344
BpuMI CCSGG 1 cut(s) 14
Bsa29I ATCGAT 1 cut(s) 147
BsaHI GRCGYC 3 cut(s) 182, 193, 492
BsaI GGTCTC 2 cut(s) 92, 410
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 2 cut(s) 167, 320
BsaXI ACNNNNNCTCC 4 cut(s) 299, 329, 336, 366
Bsc4I CCNNNNNNNGG 3 cut(s) 367, 393, 447
Bse118I RCCGGY 1 cut(s) 320
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 2 cut(s) 208, 218
BseLI CCNNNNNNNGG 3 cut(s) 367, 393, 447
BseRI GAGGAG 1 cut(s) 360
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 1 cut(s) 501
BseYI CCCAGC 1 cut(s) 272
BshFI GGCC 2 cut(s) 277, 383
BshTI ACCGGT 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 147
BsiHKAI GWGCWC 1 cut(s) 320
BsiSI CCGG 4 cut(s) 14, 168, 179, 321
BslI CCNNNNNNNGG 3 cut(s) 367, 393, 447
BsmAI GTCTC 2 cut(s) 92, 410
BsnI GGCC 2 cut(s) 277, 383
Bso31I GGTCTC 2 cut(s) 92, 410
Bsp1286I GDGCHC 1 cut(s) 320
BspACI CCGC 5 cut(s) 54, 402, 473, 486, 512
BspANI GGCC 2 cut(s) 277, 383
BspDI ATCGAT 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 52
BspMAI CTGCAG 1 cut(s) 519
BspMI ACCTGC 1 cut(s) 508
BspTNI GGTCTC 2 cut(s) 92, 410
BsrFI RCCGGY 1 cut(s) 320
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 12
BssNI GRCGYC 3 cut(s) 182, 193, 492
BstACI GRCGYC 3 cut(s) 182, 193, 492
BstAPI GCANNNNNTGC 1 cut(s) 523
BstDEI CTNAG 1 cut(s) 117
BstF5I GGATG 2 cut(s) 208, 218
BstMAI GTCTC 2 cut(s) 92, 410
BstMWI GCNNNNNNNGC 2 cut(s) 269, 523
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 12
BstSFI CTRYAG 1 cut(s) 515
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 501
Bsu15I ATCGAT 1 cut(s) 147
BsuRI GGCC 2 cut(s) 277, 383
BsuTUI ATCGAT 1 cut(s) 147
BtsCI GGATG 2 cut(s) 208, 218
BveI ACCTGC 1 cut(s) 508
CaiI CAGNNNCTG 1 cut(s) 514
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 1 cut(s) 275
ClaI ATCGAT 1 cut(s) 147
CseI GACGC 2 cut(s) 171, 481
CspAI ACCGGT 1 cut(s) 320
CviAII CATG 2 cut(s) 70, 534
DdeI CTNAG 1 cut(s) 117
EaeI YGGCCR 1 cut(s) 381
Eco31I GGTCTC 2 cut(s) 92, 410
FaeI CATG 2 cut(s) 73, 537
FaiI YATR 9 cut(s) 71, 76, 96, 137, 155, 223, 225, 255, 535
FatI CATG 2 cut(s) 69, 533
FauNDI CATATG 1 cut(s) 223
Fnu4HI GCNGC 3 cut(s) 474, 512, 515
FokI GGATG 2 cut(s) 215, 225
Fsp4HI GCNGC 3 cut(s) 474, 512, 515
FspBI CTAG 1 cut(s) 35
GluI GCNGC 3 cut(s) 474, 512, 515
GsaI CCCAGC 1 cut(s) 276
GsuI CTGGAG 2 cut(s) 326, 344
HaeIII GGCC 2 cut(s) 277, 383
HapII CCGG 4 cut(s) 14, 168, 179, 321
HgaI GACGC 2 cut(s) 171, 481
Hin1I GRCGYC 3 cut(s) 182, 193, 492
Hin1II CATG 2 cut(s) 73, 537
HinfI GANTC 2 cut(s) 29, 241
HpaII CCGG 4 cut(s) 14, 168, 179, 321
HphI GGTGA 2 cut(s) 347, 509
Hpy166II GTNNAC 1 cut(s) 318
Hpy188I TCNGA 2 cut(s) 212, 285
Hpy188III TCNNGA 3 cut(s) 35, 238, 361
Hpy8I GTNNAC 1 cut(s) 318
Hpy99I CGWCG 5 cut(s) 187, 195, 198, 344, 497
HpyAV CCTTC 2 cut(s) 326, 494
HpyCH4IV ACGT 2 cut(s) 112, 193
HpyCH4V TGCA 4 cut(s) 318, 413, 460, 517
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 523
HpyF3I CTNAG 1 cut(s) 117
HpySE526I ACGT 2 cut(s) 112, 193
Hsp92I GRCGYC 3 cut(s) 182, 193, 492
Hsp92II CATG 2 cut(s) 73, 537
LmnI GCTCC 3 cut(s) 347, 363, 530
Lsp1109I GCAGC 1 cut(s) 501
LweI GCATC 1 cut(s) 400
MaeI CTAG 1 cut(s) 35
MaeII ACGT 2 cut(s) 112, 193
MaeIII GTNAC 2 cut(s) 335, 504
MboII GAAGA 2 cut(s) 50, 133
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 4 cut(s) 198, 245, 301, 338
MspA1I CMGCKG 1 cut(s) 514
MspI CCGG 4 cut(s) 14, 168, 179, 321
MspR9I CCNGG 1 cut(s) 14
MwoI GCNNNNNNNGC 2 cut(s) 269, 523
NciI CCSGG 1 cut(s) 14
NdeI CATATG 1 cut(s) 223
NlaIII CATG 2 cut(s) 73, 537
NlaIV GGNNCC 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 335, 504
NspI RCATGY 1 cut(s) 73
PaqCI CACCTGC 1 cut(s) 508
PfeI GAWTC 2 cut(s) 29, 241
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 3 cut(s) 475, 513, 516
PspFI CCCAGC 1 cut(s) 272
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 275
PstI CTGCAG 1 cut(s) 519
PstNI CAGNNNCTG 1 cut(s) 514
SatI GCNGC 3 cut(s) 474, 512, 515
Sau96I GGNCC 1 cut(s) 275
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 1 cut(s) 320
SetI ASST 9 cut(s) 115, 196, 237, 274, 312, 337, 377, 428, 522
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 1 cut(s) 515
Sse9I AATT 1 cut(s) 20
SsiI CCGC 5 cut(s) 54, 402, 473, 486, 512
SspI AATATT 1 cut(s) 128
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 12
TaiI ACGT 2 cut(s) 115, 196
TaqI TCGA 3 cut(s) 147, 190, 408
TasI AATT 1 cut(s) 20
TauI GCSGC 2 cut(s) 476, 514
TfiI GAWTC 2 cut(s) 29, 241
TseFI GTSAC 2 cut(s) 335, 504
TseI GCWGC 1 cut(s) 514
Tsp45I GTSAC 2 cut(s) 335, 504
TspDTI ATGAA 1 cut(s) 142
TspGWI ACGGA 1 cut(s) 357
VneI GTGCAC 1 cut(s) 316
XbaI TCTAGA 1 cut(s) 34
XceI RCATGY 1 cut(s) 73
XspI CTAG 1 cut(s) 35
ZraI GACGTC 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.