RLG00000014644

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004841
Physical Location & Seq
Forward (+)
55714086 .. 55718647
4562 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014644

Sequence Viewer

Length: 1785 bp
ATGCCATTAGAGCTTGCAGGCTTTCCAGTTTCCACCCCCCACGGTGCTTATGTATTGTGCGAGAAGACTTTAGATGCCTTTGAGATTTTGGGTATGAATGTTGGATTCTTACGTGCCAGGCTGAAGCGGCTTCTGAGCCTTTCTTTCAGGACCAAACAGGCTCTGATAAGGAAATCAAAGGAGGCTAAACTTGAGCAAGCTCGTGCAAAGGACAAGGTGATAACTCTTGAATCCAAGTATTGGAAATGGAAAGAGAGGATGGTAAGTGCTGATGCTAAGATAAAGGCTCTAAAGGAAGTTGCAGAGAGGCGTGAACTTTTCTTCAGGAAGAGGCTGTTGCTCCATGGGGACGAGAAGAATTCCGGCTCCCATTTTAGGCAAGTTTCTAGTCCCCTTGCGTGTCTCACTTGCAATCTTTCTCTATTTTACACTACACTATTCAAGCTCAGAGTCCTAAGAGAACCCATGGCTATTGTAAAAGAACAGAAACAGAGCTCCAAGGGTTTTGCTTTGCAGAATAAGAGGGCAAGATCATCAAACAATGAAGATGAGCACACGATTGGAACCAGTGTGAGAGAATTACGCTCTGTTTTTTATTACTTTAGCAAACAAAAGAAATTTAAGAGAGCCACAGAAGAAAGTTTATATGAGACTTCTGAAGCTCAGTTTTCTGTTCTCGAAAGAGCAAATGAGGTTGCAGCAAACCTAGATCCTCAGTTTCCTAGCATGGTCAAAGTTATGTTGCCATCACATGTCTCAGGAGGATTCTGGCTTGGTCTTTCTAAAACATTCTGCATTAAGCATTTGCCAAAGCAAGACACAATGTTTGTTTTAGAAGATGAAAATGGCGAGAAGTTTGAAACGAAGTATCTTGTCGAAAAGATAGGACTGAGTGGTGGATGGAGAGGGTTTTCAATTGCTCACAAGTTGCTGGCTGGAGATGTCTTAGTTTTCCATCTAGTCACAGAATCTAAGTTCAAGGTTTATGTTATTAGATCAAATGGCTTAGGTGAAGTGGAATGCGCTCTTGGCCTTGTCCAATTAGATGCTGGCATTCGACCGGTAGATTCTGCAGATACTAATGCATGTGAAAGGGCAGAAAATGAGAATAGGGAACCTACATCAGCGGAAAATCCTGATGGAGTTATTCAAAGGAACAAAACCGCAGCCTGTAATAGTAAACGTGGTACAGTGTCAGATCTGTTTGAAGATGACACTGAAGATGCTAGTTGTGAAGTTTTGGAAGGAATCAAGCTTTCAGAGTCTATTGTCCCTTTTGATGAAGTGTGCGGCATACAGAACTTCAATGTCATCTACAATGGTTTAATCATAAATCCCCTGTTTCCGAAACCCCTTCTGACCAAGTACTATGAGCTCTGCTGCAGTCAAAACTCTTTCCTTCATGAGCACCTTCTTGAGGGTCTTAACTACAAACTGGTAGCTGGAATAATTTCCGAAACAACCAATATTGCAGATGCCATTGAAGCCTGCAAGATCACTACCTTGGAGAGTAGTTTCTCAACTTGGGATAAGACTTTGCAAGCCGGCGAGATGTTAGGCATGAATGTCAGCTTCTTGCGGGAGCGACTTGAACAGCTTGCGAGTCTTGCTTCAAAATTAAAAAGGTGTGAAGAGGCTAGACTGGAAAGGGAGCGAGAAGAACAAGAGATGAGAACCAGGTTGGCAAAGCTTTTAGAAGTAAAAGAGAAGAAGGATAGGCTACAGTCTCGGATTTCAGTGAAGCTTATGTCCAGGTTCAAAGAAGTAATCGACGCTCCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

595

Amino Acids

67.46

Weight (kDa)

8.59

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 244 - 333 9.9e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 240
AciI CCGC 5 cut(s) 127, 1127, 1164, 1290, 1579
AclWI GGATC 1 cut(s) 704
AcsI RAATTY 2 cut(s) 358, 617
AcuI CTGAAG 4 cut(s) 143, 307, 678, 1239
AfaI GTAC 2 cut(s) 1189, 1367
AfiI CCNNNNNNNGG 3 cut(s) 240, 375, 1417
AflIII ACRYGT 1 cut(s) 751
AgeI ACCGGT 1 cut(s) 1060
AjnI CCWGG 3 cut(s) 116, 1676, 1751
AjuI GAANNNNNNNTTGG 2 cut(s) 1011, 1043
Alw21I GWGCWC 4 cut(s) 497, 555, 1377, 1410
Alw26I GTCTC 4 cut(s) 407, 644, 760, 1731
AlwI GGATC 1 cut(s) 704
AlwNI CAGNNNCTG 1 cut(s) 163
AoxI GGCC 1 cut(s) 1030
ApeKI GCWGC 3 cut(s) 698, 1166, 1380
ApoI RAATTY 2 cut(s) 358, 617
ArsI GACNNNNNNTTYG 2 cut(s) 809, 841
AsiGI ACCGGT 1 cut(s) 1060
Asp700I GAANNNNTTC 1 cut(s) 1450
AspLEI GCGC 1 cut(s) 1025
AspS9I GGNCC 1 cut(s) 150
AsuHPI GGTGA 2 cut(s) 229, 1022
AvaII GGWCC 1 cut(s) 150
BanII GRGCYC 2 cut(s) 497, 1377
BauI CACGAG 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 71
Bbv12I GWGCWC 4 cut(s) 497, 555, 1377, 1410
BbvI GCAGC 3 cut(s) 710, 1178, 1367
BccI CCATC 5 cut(s) 253, 754, 894, 963, 1133
BciT130I CCWGG 3 cut(s) 118, 1678, 1753
BcoDI GTCTC 4 cut(s) 407, 644, 760, 1731
BfaI CTAG 6 cut(s) 387, 707, 723, 959, 1227, 1638
BfmI CTRYAG 3 cut(s) 1071, 1381, 1721
BglII AGATCT 1 cut(s) 1198
BisI GCNGC 5 cut(s) 128, 699, 1167, 1291, 1381
BlsI GCNGC 5 cut(s) 129, 700, 1168, 1292, 1382
BmcAI AGTACT 1 cut(s) 1367
Bme1390I CCNGG 3 cut(s) 118, 1678, 1753
Bme18I GGWCC 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 3 cut(s) 367, 565, 1116
BmrFI CCNGG 3 cut(s) 118, 1678, 1753
BmsI GCATC 5 cut(s) 64, 262, 1036, 1213, 1465
BpiI GAAGAC 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 957
Bpu10I CCTNAGC 1 cut(s) 1006
BpuEI CTTGAG 2 cut(s) 212, 1436
BsaAI YACGTR 1 cut(s) 113
BsaJI CCNNGG 6 cut(s) 40, 343, 465, 498, 1503, 1778
BsaWI WCCGGW 1 cut(s) 1060
Bsc4I CCNNNNNNNGG 3 cut(s) 240, 375, 1417
Bse118I RCCGGY 2 cut(s) 1060, 1544
Bse1I ACTGG 4 cut(s) 26, 567, 1440, 1647
BseBI CCWGG 3 cut(s) 118, 1678, 1753
BseDI CCNNGG 6 cut(s) 40, 343, 465, 498, 1503, 1778
BseGI GGATG 2 cut(s) 264, 905
BseLI CCNNNNNNNGG 3 cut(s) 240, 375, 1417
BseMII CTCAG 6 cut(s) 125, 460, 677, 728, 771, 881
BseNI ACTGG 4 cut(s) 26, 567, 1440, 1647
BseXI GCAGC 3 cut(s) 710, 1178, 1367
Bsh1285I CGRYCG 1 cut(s) 1061
BshFI GGCC 1 cut(s) 1032
BshTI ACCGGT 1 cut(s) 1060
BsiEI CGRYCG 1 cut(s) 1061
BsiHKAI GWGCWC 4 cut(s) 497, 555, 1377, 1410
BsiSI CCGG 3 cut(s) 363, 1061, 1545
BslFI GGGAC 3 cut(s) 362, 375, 1256
BslI CCNNNNNNNGG 3 cut(s) 240, 375, 1417
BsmAI GTCTC 4 cut(s) 407, 644, 760, 1731
BsmFI GGGAC 3 cut(s) 362, 375, 1256
BsmI GAATGC 2 cut(s) 1025, 1053
BsnI GGCC 1 cut(s) 1032
Bsp1286I GDGCHC 4 cut(s) 497, 555, 1377, 1410
Bsp143I GATC 5 cut(s) 530, 709, 995, 1198, 1494
Bsp19I CCATGG 2 cut(s) 343, 465
BspACI CCGC 5 cut(s) 127, 1127, 1164, 1290, 1579
BspANI GGCC 1 cut(s) 1032
BspCNI CTCAG 6 cut(s) 126, 459, 676, 727, 770, 882
BspHI TCATGA 1 cut(s) 1402
BspLI GGNNCC 3 cut(s) 367, 565, 1116
BspMAI CTGCAG 2 cut(s) 1075, 1385
BspPI GGATC 1 cut(s) 704
BsrFI RCCGGY 2 cut(s) 1060, 1544
BsrI ACTGG 4 cut(s) 26, 567, 1440, 1647
BssAI RCCGGY 2 cut(s) 1060, 1544
BssECI CCNNGG 6 cut(s) 40, 343, 465, 498, 1503, 1778
BssMI GATC 5 cut(s) 530, 709, 995, 1198, 1494
BssSI CACGAG 1 cut(s) 201
BssT1I CCWWGG 5 cut(s) 343, 465, 498, 1503, 1778
Bst2BI CACGAG 1 cut(s) 201
Bst2UI CCWGG 3 cut(s) 118, 1678, 1753
Bst4CI ACNGT 3 cut(s) 44, 1192, 1725
Bst6I CTCTTC 2 cut(s) 323, 1626
BstBAI YACGTR 1 cut(s) 113
BstC8I GCNNGC 9 cut(s) 15, 19, 198, 933, 1051, 1489, 1542, 1546, 1599
BstDSI CCRYGG 3 cut(s) 40, 343, 465
BstENI CCTNNNNNAGG 1 cut(s) 1415
BstF5I GGATG 2 cut(s) 264, 905
BstHHI GCGC 1 cut(s) 1025
BstKTI GATC 5 cut(s) 533, 712, 998, 1201, 1497
BstMAI GTCTC 4 cut(s) 407, 644, 760, 1731
BstMBI GATC 5 cut(s) 530, 709, 995, 1198, 1494
BstMCI CGRYCG 1 cut(s) 1061
BstMWI GCNNNNNNNGC 5 cut(s) 10, 127, 1029, 1484, 1607
BstNI CCWGG 3 cut(s) 118, 1678, 1753
BstNSI RCATGY 2 cut(s) 755, 1089
BstSCI CCNGG 3 cut(s) 116, 1676, 1751
BstSFI CTRYAG 3 cut(s) 1071, 1381, 1721
BstV1I GCAGC 3 cut(s) 710, 1178, 1367
BstV2I GAAGAC 1 cut(s) 71
BstX2I RGATCY 2 cut(s) 709, 1198
BstYI RGATCY 2 cut(s) 709, 1198
BsuRI GGCC 1 cut(s) 1032
BtgI CCRYGG 3 cut(s) 40, 343, 465
BtsCI GGATG 2 cut(s) 264, 905
BtsIMutI CAGTG 4 cut(s) 574, 1197, 1215, 1743
Cac8I GCNNGC 9 cut(s) 15, 19, 198, 933, 1051, 1489, 1542, 1546, 1599
CaiI CAGNNNCTG 1 cut(s) 163
CciI TCATGA 1 cut(s) 1402
CfoI GCGC 1 cut(s) 1025
Cfr10I RCCGGY 2 cut(s) 1060, 1544
Cfr13I GGNCC 1 cut(s) 150
CsiI ACCWGGT 1 cut(s) 1676
Csp6I GTAC 2 cut(s) 1188, 1366
CspAI ACCGGT 1 cut(s) 1060
CviAII CATG 7 cut(s) 344, 466, 727, 752, 1086, 1403, 1561
CviQI GTAC 2 cut(s) 1188, 1366
DpnI GATC 5 cut(s) 532, 711, 997, 1200, 1496
DpnII GATC 5 cut(s) 530, 709, 995, 1198, 1494
Eam1104I CTCTTC 2 cut(s) 323, 1626
EarI CTCTTC 2 cut(s) 323, 1626
Ecl136II GAGCTC 2 cut(s) 495, 1375
Eco130I CCWWGG 5 cut(s) 343, 465, 498, 1503, 1778
Eco24I GRGCYC 2 cut(s) 497, 1377
Eco47I GGWCC 1 cut(s) 150
Eco53kI GAGCTC 2 cut(s) 495, 1375
Eco57I CTGAAG 4 cut(s) 143, 307, 678, 1239
EcoICRI GAGCTC 2 cut(s) 495, 1375
EcoNI CCTNNNNNAGG 1 cut(s) 1415
EcoRI GAATTC 1 cut(s) 358
EcoRII CCWGG 3 cut(s) 116, 1676, 1751
EcoT14I CCWWGG 5 cut(s) 343, 465, 498, 1503, 1778
EcoT22I ATGCAT 1 cut(s) 1087
EcoT38I GRGCYC 2 cut(s) 497, 1377
ErhI CCWWGG 5 cut(s) 343, 465, 498, 1503, 1778
FaeI CATG 7 cut(s) 347, 469, 730, 755, 1089, 1406, 1564
FaqI GGGAC 3 cut(s) 362, 375, 1256
FatI CATG 7 cut(s) 343, 465, 726, 751, 1085, 1402, 1560
FauI CCCGC 1 cut(s) 1572
Fnu4HI GCNGC 5 cut(s) 128, 699, 1167, 1291, 1381
FokI GGATG 2 cut(s) 271, 912
FriOI GRGCYC 2 cut(s) 497, 1377
Fsp4HI GCNGC 5 cut(s) 128, 699, 1167, 1291, 1381
FspBI CTAG 6 cut(s) 387, 707, 723, 959, 1227, 1638
GlaI GCGC 1 cut(s) 1024
GluI GCNGC 5 cut(s) 128, 699, 1167, 1291, 1381
GsuI CTGGAG 1 cut(s) 957
HaeIII GGCC 1 cut(s) 1032
HapII CCGG 3 cut(s) 363, 1061, 1545
HhaI GCGC 1 cut(s) 1025
Hin1II CATG 7 cut(s) 347, 469, 730, 755, 1089, 1406, 1564
Hin6I GCGC 1 cut(s) 1023
HinP1I GCGC 1 cut(s) 1023
HindIII AAGCTT 3 cut(s) 1253, 1688, 1742
HinfI GANTC 9 cut(s) 105, 230, 450, 765, 968, 1067, 1248, 1262, 1603
HpaII CCGG 3 cut(s) 363, 1061, 1545
HphI GGTGA 2 cut(s) 229, 1022
Hpy166II GTNNAC 2 cut(s) 314, 1181
Hpy188III TCNNGA 8 cut(s) 148, 227, 325, 677, 759, 1136, 1403, 1415
Hpy8I GTNNAC 2 cut(s) 314, 1181
Hpy99I CGWCG 1 cut(s) 1775
HpyAV CCTTC 5 cut(s) 1238, 1364, 1409, 1421, 1705
HpyCH4III ACNGT 3 cut(s) 44, 1192, 1725
HpyCH4IV ACGT 2 cut(s) 112, 1183
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 127, 1029, 1484, 1607
HpySE526I ACGT 2 cut(s) 112, 1183
Hsp92II CATG 7 cut(s) 347, 469, 730, 755, 1089, 1406, 1564
HspAI GCGC 1 cut(s) 1023
KroI GCCGGC 1 cut(s) 1544
KroNI GCCGGC 1 cut(s) 1546
Kzo9I GATC 5 cut(s) 530, 709, 995, 1198, 1494
LmnI GCTCC 6 cut(s) 345, 371, 500, 1582, 1651, 1780
Lsp1109I GCAGC 3 cut(s) 710, 1178, 1367
LweI GCATC 5 cut(s) 64, 262, 1036, 1213, 1465
MabI ACCWGGT 1 cut(s) 1676
MaeI CTAG 6 cut(s) 387, 707, 723, 959, 1227, 1638
MaeII ACGT 2 cut(s) 112, 1183
MaeIII GTNAC 1 cut(s) 961
MalI GATC 5 cut(s) 532, 711, 997, 1200, 1496
MboI GATC 5 cut(s) 530, 709, 995, 1198, 1494
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 2 cut(s) 709, 1198
MhlI GDGCHC 4 cut(s) 497, 555, 1377, 1410
MluCI AATT 7 cut(s) 358, 578, 617, 915, 1040, 1449, 1616
MlyI GAGTC 3 cut(s) 459, 1271, 1612
MmeI TCCRAC 1 cut(s) 82
Mph1103I ATGCAT 1 cut(s) 1087
MroNI GCCGGC 1 cut(s) 1544
MroXI GAANNNNTTC 1 cut(s) 1450
MseI TTAA 5 cut(s) 621, 798, 1325, 1425, 1619
MspA1I CMGCKG 1 cut(s) 1127
MspI CCGG 3 cut(s) 363, 1061, 1545
MspR9I CCNGG 3 cut(s) 118, 1678, 1753
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 2 cut(s) 1025, 1053
MvaI CCWGG 3 cut(s) 118, 1678, 1753
MwoI GCNNNNNNNGC 5 cut(s) 10, 127, 1029, 1484, 1607
NaeI GCCGGC 1 cut(s) 1546
NcoI CCATGG 2 cut(s) 343, 465
NdeII GATC 5 cut(s) 530, 709, 995, 1198, 1494
NgoMIV GCCGGC 1 cut(s) 1544
NlaIII CATG 7 cut(s) 347, 469, 730, 755, 1089, 1406, 1564
NlaIV GGNNCC 3 cut(s) 367, 565, 1116
NmuCI GTSAC 1 cut(s) 961
NsiI ATGCAT 1 cut(s) 1087
NspI RCATGY 2 cut(s) 755, 1089
PagI TCATGA 1 cut(s) 1402
PciI ACATGT 1 cut(s) 751
PctI GAATGC 2 cut(s) 1025, 1053
PdiI GCCGGC 1 cut(s) 1546
PdmI GAANNNNTTC 1 cut(s) 1450
PfeI GAWTC 6 cut(s) 105, 230, 765, 968, 1067, 1248
PflMI CCANNNNNTGG 1 cut(s) 240
PinAI ACCGGT 1 cut(s) 1060
PkrI GCNGC 5 cut(s) 129, 700, 1168, 1292, 1382
PleI GAGTC 3 cut(s) 458, 1270, 1611
PpsI GAGTC 3 cut(s) 458, 1270, 1611
Ppu21I YACGTR 1 cut(s) 113
PscI ACATGT 1 cut(s) 751
Psp124BI GAGCTC 2 cut(s) 497, 1377
Psp6I CCWGG 3 cut(s) 116, 1676, 1751
PspGI CCWGG 3 cut(s) 116, 1676, 1751
PspN4I GGNNCC 3 cut(s) 367, 565, 1116
PspPI GGNCC 1 cut(s) 150
PstI CTGCAG 2 cut(s) 1075, 1385
PstNI CAGNNNCTG 1 cut(s) 163
PsuI RGATCY 2 cut(s) 709, 1198
RsaI GTAC 2 cut(s) 1189, 1367
RsaNI GTAC 2 cut(s) 1188, 1366
SacI GAGCTC 2 cut(s) 497, 1377
SaqAI TTAA 5 cut(s) 621, 798, 1325, 1425, 1619
SatI GCNGC 5 cut(s) 128, 699, 1167, 1291, 1381
Sau3AI GATC 5 cut(s) 530, 709, 995, 1198, 1494
Sau96I GGNCC 1 cut(s) 150
ScaI AGTACT 1 cut(s) 1367
SchI GAGTC 3 cut(s) 459, 1271, 1612
ScrFI CCNGG 3 cut(s) 118, 1678, 1753
SduI GDGCHC 4 cut(s) 497, 555, 1377, 1410
SexAI ACCWGGT 1 cut(s) 1676
SfaNI GCATC 5 cut(s) 64, 262, 1036, 1213, 1465
SfcI CTRYAG 3 cut(s) 1071, 1381, 1721
SinI GGWCC 1 cut(s) 150
SmlI CTYRAG 2 cut(s) 191, 1415
SmoI CTYRAG 2 cut(s) 191, 1415
Sse9I AATT 7 cut(s) 358, 578, 617, 915, 1040, 1449, 1616
SsiI CCGC 5 cut(s) 127, 1127, 1164, 1290, 1579
SspI AATATT 1 cut(s) 1468
SspMI CTAG 6 cut(s) 387, 707, 723, 959, 1227, 1638
SstI GAGCTC 2 cut(s) 497, 1377
StyD4I CCNGG 3 cut(s) 116, 1676, 1751
StyI CCWWGG 5 cut(s) 343, 465, 498, 1503, 1778
TaaI ACNGT 3 cut(s) 44, 1192, 1725
TaiI ACGT 2 cut(s) 115, 1186
TaqI TCGA 4 cut(s) 678, 876, 1057, 1770
TasI AATT 7 cut(s) 358, 578, 617, 915, 1040, 1449, 1616
TatI WGTACW 1 cut(s) 1365
TauI GCSGC 2 cut(s) 130, 1293
TfiI GAWTC 6 cut(s) 105, 230, 765, 968, 1067, 1248
Tru1I TTAA 5 cut(s) 621, 798, 1325, 1425, 1619
Tru9I TTAA 5 cut(s) 621, 798, 1325, 1425, 1619
TscAI CASTG 4 cut(s) 574, 1197, 1222, 1743
TseFI GTSAC 1 cut(s) 961
TseI GCWGC 3 cut(s) 698, 1166, 1380
Tsp45I GTSAC 1 cut(s) 961
TspDTI ATGAA 6 cut(s) 110, 558, 855, 1296, 1391, 1577
TspRI CASTG 4 cut(s) 574, 1197, 1222, 1743
Van91I CCANNNNNTGG 1 cut(s) 240
VpaK11BI GGWCC 1 cut(s) 150
XagI CCTNNNNNAGG 1 cut(s) 1415
XapI RAATTY 2 cut(s) 358, 617
XceI RCATGY 2 cut(s) 755, 1089
XcmI CCANNNNNNNNNTGG 1 cut(s) 1046
XmnI GAANNNNTTC 1 cut(s) 1450
XspI CTAG 6 cut(s) 387, 707, 723, 959, 1227, 1638
ZrmI AGTACT 1 cut(s) 1367
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.