Rroxscaffold_7G00208180

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004841
Physical Location & Seq
Forward (+)
58171766 .. 58174005
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00208180.1

Sequence Viewer

Length: 963 bp
ATGTTTGTTTTAGAAGATGAAAATGGCGAGAAGTTCGAAACGAAGTATCTTGTCAAAAAGATAGGACTGAGTGGTGGATGGAGAGGGTTTTCAATTGCTCACAAGTTGCTGGCTGGAGATGTCTTAGTTTTCCATCTAGTCACAGAATCTAAGTTCAAGGTTTATGTTATTAGATCAAATGGCTTAGGTGAAGTGGAATGCACTCTTGGCCTTGTCCAATTAGATGCTGGCATTCGACCAGTAGATTCTGCAGATACTAATGCATATGAAAGGGCAGAAAATGAGAATAGGGAACCTACATCAGTGGAAAATCCTGATCGAATTATTCAAAGGAACAAAACCACAGCCTGTAATAGTAAACGTGGTACAGTGTCAGATCTGTTTGAAGATGACACTGAAGATGCTACTTGTGAAGTTTTGGAAGGAATCAAGCTTTCAGAGTCAATTGTCCCTTTTGATGAAGTGTGCGGCATAGAGAACTTCAATGTCATCTACAATGGTTTAATCATAAATCCCCTGTTTCCGAAACCCCTTCTGACCAAGTACTATGAGCTCTGCTGCAGTCAAAACTCTTTCCTTCATGAGCACCTTCTTGAGGGTCTTAACTACAAACTGGTAGCTGGAATAATTTCCGAAACAACCAATATTGCAGATGCCATTGAAGCTTGCAAGATCACTACCTTGGAGAGTAGTTTCTCAACTTGGGATAAGACTTTGCAAGCCGGCGAGATGTTAGGCATGAATGTCAGCTTCTTGCGTGAGCGACTTGAACAGCTTGCGAGTCTTGCTTCAAAATTAAAAAGGTGTGAAGAGGCTAGACTGGAACGGGAGCAAGAAGAACAAGAGATGAGAACCAGGTTGGCAAAGCTTTTAGAAGTAAAAGAGAAGAAGGATAGGCTACAGTCTCGGATTTCAGTGAAGCTTATGTCCAGGTTCAAAGAAGTAATCGACGCTCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

320

Amino Acids

36.25

Weight (kDa)

5.08

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 2 - 59 2.4e-09 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 468
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 2 cut(s) 367, 545
AfiI CCNNNNNNNGG 1 cut(s) 595
AgsI TTSAA 9 cut(s) 93, 157, 329, 386, 484, 662, 770, 792, 937
AjnI CCWGG 2 cut(s) 854, 929
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 8 cut(s) 433, 553, 620, 665, 750, 775, 868, 922
AluI AGCT 8 cut(s) 433, 553, 620, 665, 750, 775, 868, 922
Alw21I GWGCWC 2 cut(s) 555, 588
Alw26I GTCTC 1 cut(s) 909
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 1 cut(s) 558
Asp700I GAANNNNTTC 1 cut(s) 628
AsuHPI GGTGA 1 cut(s) 200
AsuII TTCGAA 1 cut(s) 36
BanII GRGCYC 1 cut(s) 555
Bbv12I GWGCWC 2 cut(s) 555, 588
BbvI GCAGC 1 cut(s) 545
BccI CCATC 2 cut(s) 72, 141
BciT130I CCWGG 2 cut(s) 856, 931
BcoDI GTCTC 1 cut(s) 909
BfaI CTAG 2 cut(s) 137, 816
BfmI CTRYAG 3 cut(s) 249, 559, 899
BglII AGATCT 1 cut(s) 376
BisI GCNGC 2 cut(s) 469, 559
BlsI GCNGC 2 cut(s) 470, 560
BmcAI AGTACT 1 cut(s) 545
Bme1390I CCNGG 2 cut(s) 856, 931
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 2 cut(s) 856, 931
BmsI GCATC 3 cut(s) 214, 391, 643
BpmI CTGGAG 1 cut(s) 135
Bpu10I CCTNAGC 1 cut(s) 184
Bpu14I TTCGAA 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 614
BsaJI CCNNGG 2 cut(s) 681, 956
Bsc4I CCNNNNNNNGG 1 cut(s) 595
Bse118I RCCGGY 1 cut(s) 722
Bse1I ACTGG 3 cut(s) 239, 618, 825
BseBI CCWGG 2 cut(s) 856, 931
BseDI CCNNGG 2 cut(s) 681, 956
BseGI GGATG 1 cut(s) 83
BseLI CCNNNNNNNGG 1 cut(s) 595
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 3 cut(s) 239, 618, 825
BseXI GCAGC 1 cut(s) 545
BshFI GGCC 1 cut(s) 210
BsiHKAI GWGCWC 2 cut(s) 555, 588
BsiSI CCGG 1 cut(s) 723
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 595
BsmAI GTCTC 1 cut(s) 909
BsmFI GGGAC 1 cut(s) 434
BsmI GAATGC 2 cut(s) 203, 231
BsnI GGCC 1 cut(s) 210
Bsp119I TTCGAA 1 cut(s) 36
Bsp1286I GDGCHC 2 cut(s) 555, 588
Bsp143I GATC 4 cut(s) 173, 316, 376, 672
BspACI CCGC 1 cut(s) 468
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 1 cut(s) 60
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 1 cut(s) 294
BspMAI CTGCAG 2 cut(s) 253, 563
BspT104I TTCGAA 1 cut(s) 36
BsrFI RCCGGY 1 cut(s) 722
BsrI ACTGG 3 cut(s) 239, 618, 825
BssAI RCCGGY 1 cut(s) 722
BssECI CCNNGG 2 cut(s) 681, 956
BssMI GATC 4 cut(s) 173, 316, 376, 672
BssT1I CCWWGG 2 cut(s) 681, 956
Bst2UI CCWGG 2 cut(s) 856, 931
Bst4CI ACNGT 2 cut(s) 370, 903
Bst6I CTCTTC 1 cut(s) 804
BstBI TTCGAA 1 cut(s) 36
BstC8I GCNNGC 6 cut(s) 111, 229, 667, 720, 724, 777
BstDEI CTNAG 4 cut(s) 68, 124, 150, 184
BstENI CCTNNNNNAGG 1 cut(s) 593
BstF5I GGATG 1 cut(s) 83
BstKTI GATC 4 cut(s) 176, 319, 379, 675
BstMAI GTCTC 1 cut(s) 909
BstMBI GATC 4 cut(s) 173, 316, 376, 672
BstMWI GCNNNNNNNGC 3 cut(s) 207, 662, 785
BstNI CCWGG 2 cut(s) 856, 931
BstSCI CCNGG 2 cut(s) 854, 929
BstSFI CTRYAG 3 cut(s) 249, 559, 899
BstV1I GCAGC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 1 cut(s) 210
BtsCI GGATG 1 cut(s) 83
BtsIMutI CAGTG 4 cut(s) 309, 375, 393, 921
Cac8I GCNNGC 6 cut(s) 111, 229, 667, 720, 724, 777
CciI TCATGA 1 cut(s) 580
Cfr10I RCCGGY 1 cut(s) 722
CsiI ACCWGGT 1 cut(s) 854
Csp6I GTAC 2 cut(s) 366, 544
CviAII CATG 2 cut(s) 581, 739
CviQI GTAC 2 cut(s) 366, 544
DdeI CTNAG 4 cut(s) 68, 124, 150, 184
DpnI GATC 4 cut(s) 175, 318, 378, 674
DpnII GATC 4 cut(s) 173, 316, 376, 672
Eam1104I CTCTTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 804
Ecl136II GAGCTC 1 cut(s) 553
Eco130I CCWWGG 2 cut(s) 681, 956
Eco24I GRGCYC 1 cut(s) 555
Eco53kI GAGCTC 1 cut(s) 553
Eco57I CTGAAG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 553
EcoNI CCTNNNNNAGG 1 cut(s) 593
EcoRII CCWGG 2 cut(s) 854, 929
EcoT14I CCWWGG 2 cut(s) 681, 956
EcoT22I ATGCAT 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 555
ErhI CCWWGG 2 cut(s) 681, 956
FaeI CATG 2 cut(s) 584, 742
FaiI YATR 9 cut(s) 165, 265, 267, 473, 509, 549, 582, 740, 926
FaqI GGGAC 1 cut(s) 434
FatI CATG 2 cut(s) 580, 738
FauNDI CATATG 1 cut(s) 265
Fnu4HI GCNGC 2 cut(s) 469, 559
FokI GGATG 1 cut(s) 90
FriOI GRGCYC 1 cut(s) 555
Fsp4HI GCNGC 2 cut(s) 469, 559
FspBI CTAG 2 cut(s) 137, 816
GluI GCNGC 2 cut(s) 469, 559
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 723
Hin1II CATG 2 cut(s) 584, 742
HindIII AAGCTT 4 cut(s) 431, 663, 866, 920
HinfI GANTC 5 cut(s) 146, 245, 426, 440, 781
HpaII CCGG 1 cut(s) 723
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 1 cut(s) 359
Hpy188I TCNGA 6 cut(s) 376, 439, 525, 537, 634, 909
Hpy188III TCNNGA 3 cut(s) 314, 581, 593
Hpy8I GTNNAC 1 cut(s) 359
Hpy99I CGWCG 1 cut(s) 953
HpyAV CCTTC 5 cut(s) 416, 542, 587, 599, 883
HpyCH4III ACNGT 2 cut(s) 370, 903
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 7 cut(s) 201, 251, 263, 561, 650, 669, 718
HpyF10VI GCNNNNNNNGC 3 cut(s) 207, 662, 785
HpyF3I CTNAG 4 cut(s) 68, 124, 150, 184
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 2 cut(s) 584, 742
KroI GCCGGC 1 cut(s) 722
KroNI GCCGGC 1 cut(s) 724
Kzo9I GATC 4 cut(s) 173, 316, 376, 672
LmnI GCTCC 2 cut(s) 829, 958
Lsp1109I GCAGC 1 cut(s) 545
LweI GCATC 3 cut(s) 214, 391, 643
MabI ACCWGGT 1 cut(s) 854
MaeI CTAG 2 cut(s) 137, 816
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 1 cut(s) 139
MalI GATC 4 cut(s) 175, 318, 378, 674
MboI GATC 4 cut(s) 173, 316, 376, 672
MboII GAAGA 6 cut(s) 26, 398, 410, 821, 848, 898
MfeI CAATTG 2 cut(s) 93, 444
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 2 cut(s) 555, 588
MluCI AATT 6 cut(s) 93, 218, 321, 444, 627, 794
MlyI GAGTC 2 cut(s) 449, 790
MnlI CCTC 3 cut(s) 77, 589, 805
Mph1103I ATGCAT 1 cut(s) 265
MroNI GCCGGC 1 cut(s) 722
MroXI GAANNNNTTC 1 cut(s) 628
MseI TTAA 3 cut(s) 503, 603, 797
MspI CCGG 1 cut(s) 723
MspR9I CCNGG 2 cut(s) 856, 931
MunI CAATTG 2 cut(s) 93, 444
Mva1269I GAATGC 2 cut(s) 203, 231
MvaI CCWGG 2 cut(s) 856, 931
MwoI GCNNNNNNNGC 3 cut(s) 207, 662, 785
NaeI GCCGGC 1 cut(s) 724
NdeI CATATG 1 cut(s) 265
NdeII GATC 4 cut(s) 173, 316, 376, 672
NgoMIV GCCGGC 1 cut(s) 722
NlaIII CATG 2 cut(s) 584, 742
NlaIV GGNNCC 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 139
NsiI ATGCAT 1 cut(s) 265
NspV TTCGAA 1 cut(s) 36
PagI TCATGA 1 cut(s) 580
PctI GAATGC 2 cut(s) 203, 231
PdiI GCCGGC 1 cut(s) 724
PdmI GAANNNNTTC 1 cut(s) 628
PfeI GAWTC 3 cut(s) 146, 245, 426
PkrI GCNGC 2 cut(s) 470, 560
PleI GAGTC 2 cut(s) 448, 789
PpsI GAGTC 2 cut(s) 448, 789
Psp124BI GAGCTC 1 cut(s) 555
Psp6I CCWGG 2 cut(s) 854, 929
PspGI CCWGG 2 cut(s) 854, 929
PspN4I GGNNCC 1 cut(s) 294
PstI CTGCAG 2 cut(s) 253, 563
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 2 cut(s) 367, 545
RsaNI GTAC 2 cut(s) 366, 544
SacI GAGCTC 1 cut(s) 555
SaqAI TTAA 3 cut(s) 503, 603, 797
SatI GCNGC 2 cut(s) 469, 559
Sau3AI GATC 4 cut(s) 173, 316, 376, 672
ScaI AGTACT 1 cut(s) 545
SchI GAGTC 2 cut(s) 449, 790
ScrFI CCNGG 2 cut(s) 856, 931
SduI GDGCHC 2 cut(s) 555, 588
SexAI ACCWGGT 1 cut(s) 854
SfaNI GCATC 3 cut(s) 214, 391, 643
SfcI CTRYAG 3 cut(s) 249, 559, 899
SfuI TTCGAA 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
Sse9I AATT 6 cut(s) 93, 218, 321, 444, 627, 794
SsiI CCGC 1 cut(s) 468
SspI AATATT 1 cut(s) 646
SspMI CTAG 2 cut(s) 137, 816
SstI GAGCTC 1 cut(s) 555
StyD4I CCNGG 2 cut(s) 854, 929
StyI CCWWGG 2 cut(s) 681, 956
TaaI ACNGT 2 cut(s) 370, 903
TaiI ACGT 1 cut(s) 364
TaqI TCGA 4 cut(s) 36, 235, 319, 948
TasI AATT 6 cut(s) 93, 218, 321, 444, 627, 794
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 471
TfiI GAWTC 3 cut(s) 146, 245, 426
Tru1I TTAA 3 cut(s) 503, 603, 797
Tru9I TTAA 3 cut(s) 503, 603, 797
TscAI CASTG 4 cut(s) 309, 375, 400, 921
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 1 cut(s) 558
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 5 cut(s) 33, 282, 474, 569, 755
TspRI CASTG 4 cut(s) 309, 375, 400, 921
XagI CCTNNNNNAGG 1 cut(s) 593
XcmI CCANNNNNNNNNTGG 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 628
XspI CTAG 2 cut(s) 137, 816
ZrmI AGTACT 1 cut(s) 545
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.