Rh6BG087500

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004841
Physical Location & Seq
Reverse (-)
13599850 .. 13603457
3608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG087500.1

Sequence Viewer

Length: 1284 bp
ATGGCTATTGTAAAAGAACAGAAACAGAGCTCCAAGAATAAGAGAGCAAGATCATCAAACAATGAAGAAAAGCACACGATTAGAACCAATGTGATCAATGTTGATTCAGTGAAGTCTGATAGTAGCAAACGAAAGAAATTTAAGAGAGCCACAGAAGAAAGTTTATATGAGACTTCTGAAGCTCAGTTTTCTGTTCTCGAAAGAGCAAATGAGGTTGCAGCAAACCTAGATCCTCAGTTTCCTAGCATGGTCAAAGTTATGTTGCCATCACATGTCTCAGGAGGATTCTGGCTTGGTCTTTCTAAAACATTCTGCATTAAGCATTTGCCAAAGCAAGACACAATGTTTGTTTTAGAAGATGAAAATGGCAAGAAGTTCGAAACGAAGTATCTTGTTGAAAAGATAGGACTGAGTGGTGGATGGAGAGGGTTTTCAATTGCTCACAAGTTGCTGGCTGGAGATGTCTTAGTTTTTCATCTAGTCACAGAATCTAAGTTCAAGGTTTATGTTATTAGATCAAATGGCTTAGGTGAAGTGGAATGCGCTCTTGGCCTTGTCCAATTAGATGCTGACATTCGACCAGTAGATTCTGCACTAATGCATGTGAAAGGGCAGAAAATGAGAATAGGGAACCTACATCAGTGGAAAATCCTGATGGAGTTATTCAAAGGAACAAAACCGCAGCCTCTTTCAGAGTCTATTGTCCCTTTTGATGAAGTGTGCGGCATAGAGAACTTCAATGTCATCTACAATGGTTTAATCATAAATCCCCTGTTTCCGAAACCCCTTCTGACCAAGTACTATGAGCTCTGCTGCAGTCAAAACTCTTTCCTTCATGAGCACCTTCTTGTGGGTCTTAACTACAAACTGGTAGCTGGAATAATTTCCGAAACAACCAATATTGCAGATGCCATTAAAGCCTGCAAGATCACTACCTTGGAGAGTAGTTTCTCAACTTGGGATAAGACTTTGCAAGCCGGCGAGATGTTAGGCATGAATGTCAACTTCCTGCGTGAGCAACTTGAACAGCTTGCGAGTCTTGCTTCAAAATTAAAAACGTGTGAAGAGGCTAGACTGGAAAGGGAGCAAGAAGAACAAGAGATGAGAACCAGGTTGGCAAAGCTTTTAGAAGTAAAAGAGAAGAAGGATAGGCTACAGTCTCGAATTTCAGTGAAGCTTATGTCCAGGTTCAAAGAAGTAATCGATGCTCCTTGGTATGCAAAGTTGGAGATGTGTGGCAGTGCATTGGGACTCTCGGCTGAAGAGTTGTCAACTATAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

427

Amino Acids

48.26

Weight (kDa)

8.59

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 84 - 173 2.6e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 680, 723
AclWI GGATC 1 cut(s) 224
AcsI RAATTY 2 cut(s) 137, 1164
AcuI CTGAAG 2 cut(s) 198, 1281
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 1 cut(s) 850
AflIII ACRYGT 2 cut(s) 271, 1058
AgsI TTSAA 8 cut(s) 398, 435, 499, 667, 739, 1025, 1047, 1192
AjnI CCWGG 2 cut(s) 1109, 1184
AjuI GAANNNNNNNTTGG 2 cut(s) 531, 563
AluBI AGCT 7 cut(s) 30, 182, 808, 875, 1030, 1123, 1177
AluI AGCT 7 cut(s) 30, 182, 808, 875, 1030, 1123, 1177
Alw21I GWGCWC 3 cut(s) 32, 810, 843
Alw26I GTCTC 3 cut(s) 164, 280, 1164
AlwI GGATC 1 cut(s) 224
AoxI GGCC 1 cut(s) 550
ApeKI GCWGC 3 cut(s) 218, 682, 813
ApoI RAATTY 2 cut(s) 137, 1164
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
Asp700I GAANNNNTTC 1 cut(s) 883
AspLEI GCGC 1 cut(s) 545
AsuHPI GGTGA 1 cut(s) 542
AsuII TTCGAA 1 cut(s) 378
BanII GRGCYC 2 cut(s) 32, 810
Bbv12I GWGCWC 3 cut(s) 32, 810, 843
BbvI GCAGC 3 cut(s) 230, 694, 800
BccI CCATC 3 cut(s) 274, 414, 649
BcgI CGANNNNNNTGC 2 cut(s) 358, 392
BciT130I CCWGG 2 cut(s) 1111, 1186
BclI TGATCA 1 cut(s) 93
BcoDI GTCTC 3 cut(s) 164, 280, 1164
BfaI CTAG 4 cut(s) 227, 243, 479, 1071
BfmI CTRYAG 2 cut(s) 814, 1154
BisI GCNGC 4 cut(s) 219, 683, 724, 814
BlsI GCNGC 4 cut(s) 220, 684, 725, 815
BmcAI AGTACT 1 cut(s) 800
Bme1390I CCNGG 2 cut(s) 1111, 1186
BmiI GGNNCC 1 cut(s) 632
BmrFI CCNGG 2 cut(s) 1111, 1186
BmsI GCATC 3 cut(s) 556, 898, 1195
BpmI CTGGAG 1 cut(s) 477
Bpu10I CCTNAGC 1 cut(s) 526
Bpu14I TTCGAA 1 cut(s) 378
Bsa29I ATCGAT 1 cut(s) 1203
BsaJI CCNNGG 2 cut(s) 936, 1211
Bsc4I CCNNNNNNNGG 1 cut(s) 850
Bse118I RCCGGY 1 cut(s) 977
Bse1I ACTGG 3 cut(s) 581, 873, 1080
BseBI CCWGG 2 cut(s) 1111, 1186
BseCI ATCGAT 1 cut(s) 1203
BseDI CCNNGG 2 cut(s) 936, 1211
BseGI GGATG 1 cut(s) 425
BseLI CCNNNNNNNGG 1 cut(s) 850
BseMII CTCAG 4 cut(s) 197, 248, 291, 401
BseNI ACTGG 3 cut(s) 581, 873, 1080
BseXI GCAGC 3 cut(s) 230, 694, 800
BsgI GTGCAG 1 cut(s) 576
BshFI GGCC 1 cut(s) 552
BshVI ATCGAT 1 cut(s) 1203
BsiHKAI GWGCWC 3 cut(s) 32, 810, 843
BsiSI CCGG 1 cut(s) 978
BslFI GGGAC 2 cut(s) 689, 1263
BslI CCNNNNNNNGG 1 cut(s) 850
BsmAI GTCTC 3 cut(s) 164, 280, 1164
BsmFI GGGAC 2 cut(s) 689, 1263
BsmI GAATGC 1 cut(s) 545
BsnI GGCC 1 cut(s) 552
Bsp119I TTCGAA 1 cut(s) 378
Bsp1286I GDGCHC 3 cut(s) 32, 810, 843
Bsp143I GATC 5 cut(s) 50, 93, 229, 515, 927
BspACI CCGC 2 cut(s) 680, 723
BspANI GGCC 1 cut(s) 552
BspCNI CTCAG 4 cut(s) 196, 247, 290, 402
BspDI ATCGAT 1 cut(s) 1203
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 1 cut(s) 632
BspMAI CTGCAG 1 cut(s) 818
BspPI GGATC 1 cut(s) 224
BspT104I TTCGAA 1 cut(s) 378
BsrFI RCCGGY 1 cut(s) 977
BsrI ACTGG 3 cut(s) 581, 873, 1080
BssAI RCCGGY 1 cut(s) 977
BssECI CCNNGG 2 cut(s) 936, 1211
BssMI GATC 5 cut(s) 50, 93, 229, 515, 927
BssT1I CCWWGG 2 cut(s) 936, 1211
Bst2UI CCWGG 2 cut(s) 1111, 1186
Bst4CI ACNGT 1 cut(s) 1158
Bst6I CTCTTC 2 cut(s) 1059, 1257
BstBI TTCGAA 1 cut(s) 378
BstC8I GCNNGC 5 cut(s) 453, 922, 975, 979, 1032
BstDEI CTNAG 7 cut(s) 183, 234, 277, 410, 466, 492, 526
BstF5I GGATG 1 cut(s) 425
BstHHI GCGC 1 cut(s) 545
BstKTI GATC 5 cut(s) 53, 96, 232, 518, 930
BstMAI GTCTC 3 cut(s) 164, 280, 1164
BstMBI GATC 5 cut(s) 50, 93, 229, 515, 927
BstMWI GCNNNNNNNGC 3 cut(s) 549, 917, 1040
BstNI CCWGG 2 cut(s) 1111, 1186
BstNSI RCATGY 2 cut(s) 275, 605
BstSCI CCNGG 2 cut(s) 1109, 1184
BstSFI CTRYAG 2 cut(s) 814, 1154
BstV1I GCAGC 3 cut(s) 230, 694, 800
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
Bsu15I ATCGAT 1 cut(s) 1203
BsuRI GGCC 1 cut(s) 552
BsuTUI ATCGAT 1 cut(s) 1203
BtsCI GGATG 1 cut(s) 425
BtsI GCAGTG 1 cut(s) 1246
BtsIMutI CAGTG 4 cut(s) 114, 647, 1176, 1246
Cac8I GCNNGC 5 cut(s) 453, 922, 975, 979, 1032
CciI TCATGA 1 cut(s) 835
CfoI GCGC 1 cut(s) 545
Cfr10I RCCGGY 1 cut(s) 977
ClaI ATCGAT 1 cut(s) 1203
CsiI ACCWGGT 1 cut(s) 1109
Csp6I GTAC 1 cut(s) 799
CviAII CATG 6 cut(s) 247, 272, 602, 836, 994, 1281
CviQI GTAC 1 cut(s) 799
DdeI CTNAG 7 cut(s) 183, 234, 277, 410, 466, 492, 526
DpnI GATC 5 cut(s) 52, 95, 231, 517, 929
DpnII GATC 5 cut(s) 50, 93, 229, 515, 927
Eam1104I CTCTTC 2 cut(s) 1059, 1257
EarI CTCTTC 2 cut(s) 1059, 1257
Ecl136II GAGCTC 2 cut(s) 30, 808
Eco130I CCWWGG 2 cut(s) 936, 1211
Eco24I GRGCYC 2 cut(s) 32, 810
Eco53kI GAGCTC 2 cut(s) 30, 808
Eco57I CTGAAG 2 cut(s) 198, 1281
EcoICRI GAGCTC 2 cut(s) 30, 808
EcoRII CCWGG 2 cut(s) 1109, 1184
EcoT14I CCWWGG 2 cut(s) 936, 1211
EcoT22I ATGCAT 1 cut(s) 603
EcoT38I GRGCYC 2 cut(s) 32, 810
ErhI CCWWGG 2 cut(s) 936, 1211
FaeI CATG 6 cut(s) 250, 275, 605, 839, 997, 1284
FaqI GGGAC 2 cut(s) 689, 1263
FatI CATG 6 cut(s) 246, 271, 601, 835, 993, 1280
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 4 cut(s) 219, 683, 724, 814
FokI GGATG 1 cut(s) 432
FriOI GRGCYC 2 cut(s) 32, 810
Fsp4HI GCNGC 4 cut(s) 219, 683, 724, 814
FspBI CTAG 4 cut(s) 227, 243, 479, 1071
GlaI GCGC 1 cut(s) 544
GluI GCNGC 4 cut(s) 219, 683, 724, 814
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 1 cut(s) 552
HapII CCGG 1 cut(s) 978
HhaI GCGC 1 cut(s) 545
Hin1II CATG 6 cut(s) 250, 275, 605, 839, 997, 1284
Hin6I GCGC 1 cut(s) 543
HinP1I GCGC 1 cut(s) 543
HincII GTYRAC 2 cut(s) 1003, 1272
HindII GTYRAC 2 cut(s) 1003, 1272
HindIII AAGCTT 2 cut(s) 1121, 1175
HinfI GANTC 7 cut(s) 104, 285, 488, 587, 695, 1036, 1251
HpaII CCGG 1 cut(s) 978
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 2 cut(s) 1003, 1272
Hpy188I TCNGA 6 cut(s) 118, 178, 694, 780, 792, 889
Hpy188III TCNNGA 5 cut(s) 197, 279, 652, 836, 1161
Hpy8I GTNNAC 2 cut(s) 1003, 1272
HpyAV CCTTC 4 cut(s) 797, 842, 854, 1138
HpyCH4III ACNGT 1 cut(s) 1158
HpyCH4IV ACGT 1 cut(s) 1058
HpyF10VI GCNNNNNNNGC 3 cut(s) 549, 917, 1040
HpyF3I CTNAG 7 cut(s) 183, 234, 277, 410, 466, 492, 526
HpySE526I ACGT 1 cut(s) 1058
Hsp92II CATG 6 cut(s) 250, 275, 605, 839, 997, 1284
HspAI GCGC 1 cut(s) 543
KroI GCCGGC 1 cut(s) 977
KroNI GCCGGC 1 cut(s) 979
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 5 cut(s) 50, 93, 229, 515, 927
LmnI GCTCC 3 cut(s) 35, 1084, 1213
Lsp1109I GCAGC 3 cut(s) 230, 694, 800
LweI GCATC 3 cut(s) 556, 898, 1195
MabI ACCWGGT 1 cut(s) 1109
MaeI CTAG 4 cut(s) 227, 243, 479, 1071
MaeII ACGT 1 cut(s) 1058
MaeIII GTNAC 1 cut(s) 481
MalI GATC 5 cut(s) 52, 95, 231, 517, 929
MboI GATC 5 cut(s) 50, 93, 229, 515, 927
MboII GAAGA 7 cut(s) 77, 167, 368, 1076, 1103, 1153, 1274
MfeI CAATTG 1 cut(s) 435
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 3 cut(s) 32, 810, 843
MluCI AATT 6 cut(s) 137, 435, 560, 882, 1049, 1164
MlyI GAGTC 3 cut(s) 704, 1045, 1245
MmeI TCCRAC 1 cut(s) 1206
MnlI CCTC 6 cut(s) 205, 243, 275, 419, 696, 1060
Mph1103I ATGCAT 1 cut(s) 603
MroNI GCCGGC 1 cut(s) 977
MroXI GAANNNNTTC 1 cut(s) 883
MseI TTAA 6 cut(s) 141, 318, 758, 858, 915, 1052
MspI CCGG 1 cut(s) 978
MspR9I CCNGG 2 cut(s) 1111, 1186
MunI CAATTG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 545
MvaI CCWGG 2 cut(s) 1111, 1186
MwoI GCNNNNNNNGC 3 cut(s) 549, 917, 1040
NaeI GCCGGC 1 cut(s) 979
NdeII GATC 5 cut(s) 50, 93, 229, 515, 927
NgoMIV GCCGGC 1 cut(s) 977
NlaIII CATG 6 cut(s) 250, 275, 605, 839, 997, 1284
NlaIV GGNNCC 1 cut(s) 632
NmeAIII GCCGAG 1 cut(s) 1235
NmuCI GTSAC 1 cut(s) 481
NsiI ATGCAT 1 cut(s) 603
NspI RCATGY 2 cut(s) 275, 605
NspV TTCGAA 1 cut(s) 378
PagI TCATGA 1 cut(s) 835
PciI ACATGT 1 cut(s) 271
PctI GAATGC 1 cut(s) 545
PdiI GCCGGC 1 cut(s) 979
PdmI GAANNNNTTC 1 cut(s) 883
PfeI GAWTC 4 cut(s) 104, 285, 488, 587
PkrI GCNGC 4 cut(s) 220, 684, 725, 815
PleI GAGTC 3 cut(s) 703, 1044, 1245
PpsI GAGTC 3 cut(s) 703, 1044, 1245
PscI ACATGT 1 cut(s) 271
Psp124BI GAGCTC 2 cut(s) 32, 810
Psp6I CCWGG 2 cut(s) 1109, 1184
PspGI CCWGG 2 cut(s) 1109, 1184
PspN4I GGNNCC 1 cut(s) 632
PstI CTGCAG 1 cut(s) 818
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
SacI GAGCTC 2 cut(s) 32, 810
SaqAI TTAA 6 cut(s) 141, 318, 758, 858, 915, 1052
SatI GCNGC 4 cut(s) 219, 683, 724, 814
Sau3AI GATC 5 cut(s) 50, 93, 229, 515, 927
ScaI AGTACT 1 cut(s) 800
SchI GAGTC 3 cut(s) 704, 1045, 1245
ScrFI CCNGG 2 cut(s) 1111, 1186
SduI GDGCHC 3 cut(s) 32, 810, 843
SexAI ACCWGGT 1 cut(s) 1109
SfaNI GCATC 3 cut(s) 556, 898, 1195
SfcI CTRYAG 2 cut(s) 814, 1154
SfuI TTCGAA 1 cut(s) 378
Sse9I AATT 6 cut(s) 137, 435, 560, 882, 1049, 1164
SsiI CCGC 2 cut(s) 680, 723
SspI AATATT 1 cut(s) 901
SspMI CTAG 4 cut(s) 227, 243, 479, 1071
SstI GAGCTC 2 cut(s) 32, 810
StyD4I CCNGG 2 cut(s) 1109, 1184
StyI CCWWGG 2 cut(s) 936, 1211
TaaI ACNGT 1 cut(s) 1158
TaiI ACGT 1 cut(s) 1061
TaqI TCGA 5 cut(s) 198, 378, 577, 1162, 1203
TasI AATT 6 cut(s) 137, 435, 560, 882, 1049, 1164
TatI WGTACW 1 cut(s) 798
TauI GCSGC 1 cut(s) 726
TfiI GAWTC 4 cut(s) 104, 285, 488, 587
Tru1I TTAA 6 cut(s) 141, 318, 758, 858, 915, 1052
Tru9I TTAA 6 cut(s) 141, 318, 758, 858, 915, 1052
TscAI CASTG 4 cut(s) 114, 647, 1176, 1246
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 3 cut(s) 218, 682, 813
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 6 cut(s) 78, 375, 464, 729, 824, 1010
TspRI CASTG 4 cut(s) 114, 647, 1176, 1246
XapI RAATTY 2 cut(s) 137, 1164
XceI RCATGY 2 cut(s) 275, 605
XmnI GAANNNNTTC 1 cut(s) 883
XspI CTAG 4 cut(s) 227, 243, 479, 1071
ZrmI AGTACT 1 cut(s) 800
Zsp2I ATGCAT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.