Rh6CG083400

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0004841
Physical Location & Seq
Reverse (-)
9098731 .. 9119438
20708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG083400.1

Sequence Viewer

Length: 1632 bp
ATGTTTGACTGTGCTTCTTTTATAGCCTACTATATATCATTCTCAAAATCAAAATCATATTTGAATCAGAAACGCATTCTGTTGTCCAGGGAAAAATCCTTATGCAAAGTTGCGAAGGAACTCGATGCTCCAGATATGGCTGATACAATAGAGATCAGAAATAGGTATTATGATCTATGCTACTCACAAAGGTCATTTATGCACAACCATCTCCTCAAAAGTATTAATGTACACTATTCAAGCTCAGAGTCCTTAAGAGAACCCATGGCTATTGTAAAAGAACAGAAACAGAGCTCCAAGAATAAGAGGGCAAGATCATCAAACAATGAAGAAAAGCACACGATTAGAACCAATGTGATCAATGTTGATTCAGTGAAGTCTGATAGTAGCAAACAAAAGAAATTTAAGAGAGCCACAGAAGAAAGTTTATATGAGACTTCTGAAGCTCAGTTTTCTGTTCTCGAAAGAGCAAATGAGGTTGCAGCAAACCTAGATCCTCAGTTTCCTAGCATGGTCAAAGTTATGTTGCCATCACATGTCTCAGGAGGATTCTGGCTTGGTCTTTCTAAAACATTCTGCATTAAGCATTTGCCAAAGCAAGACACAATGTTTGTTTTAGAAGATGAAAATGGCAAGAAGTTCGAAACGAAGTATCTTGTTGAAAAGATAGGACTGAGTGGTGGATGGAGAGGGTTTTCAATTGCTCACAAGTTGCTGGCTGGAGATGTCTTAGTTTTTCATCTAGTCACAGAATCTAAGTTCAAGGTTTATGTTATTAGATCAAATGGCTTAGGTGAAGTGGAATGCGCTCTTGGCCTTGTCCAATTAGATGCTGACATTCGACCAGTAGATTCTGATACTAATGCATGTGAAAGGGCAGAAAATGAGAATAGGGAACCTAAATCAGTGGAAAATCCTGATGGAGTTATTCAAAGGAACAAAACCGCAGCCTGTAATAGTAAACGTGGTATAGTGTCAGATCTGTTTGAAGATGACACTGAAGATGCTAGTTGTGAAGTTTTGGAAGGAATCAAGCTTTCAGAGTCTATTGTCCCTTTTGATGAAGTGTGCGGCATTGAGAACTTCAATGTCATCTACAATGGTTTAATCATAAATCCCCTGTTTCCGAAACCCCTTCTGACCAAGTACTATGAGCTCTGCTGCAGTCAAAACTCTTTCCTTCATGAGCACCTTCTTGAGGGTCTTAACTACAAACTGGTAGCTGGAATAATTTCCGAAACAACCAATATTGCAGATGCCATTAAAGCCTGCAAGATCACTACCTTGGAGAGTAGTTTCTCAACTTGGGATAAGACTTTGCAAGCCGGCGAGATGTTAGGCATGAATGTCAGCTTCCTGCGTGAGCAACTTGAACAGCTTGCGAGTCTTGCTTCAAAATTAAAAACGTGTGAAGAGGCTAGACTGGAAAGGGAGCAAGAAGAACAAGAGATGAGAACCAGGTTGGCAAAGCTTTTAGAAGTAAAAGAGAAGAAGGATAGGCTACAGTCTCGAATTTCAGTGAAGCTTATGTCCAGGTTCAAAGAAGTAATCGATGCTCCTTGGTATGCAAAGTTGGAGATGTGTGGCAGAGCATTGGGACTCTCGGCTGAAGAGTTGTCAACTATAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

543

Amino Acids

61.53

Weight (kDa)

6.17

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 172 - 261 3.9e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 945, 1071
AclWI GGATC 1 cut(s) 488
AcsI RAATTY 2 cut(s) 401, 1512
AcuI CTGAAG 3 cut(s) 462, 1020, 1629
AfaI GTAC 2 cut(s) 231, 1148
AfiI CCNNNNNNNGG 1 cut(s) 1198
AflII CTTAAG 1 cut(s) 253
AflIII ACRYGT 2 cut(s) 535, 1406
AjnI CCWGG 3 cut(s) 86, 1457, 1532
AjuI GAANNNNNNNTTGG 2 cut(s) 795, 827
Alw21I GWGCWC 3 cut(s) 296, 1158, 1191
Alw26I GTCTC 3 cut(s) 428, 544, 1512
AlwI GGATC 1 cut(s) 488
AoxI GGCC 1 cut(s) 814
ApeKI GCWGC 3 cut(s) 482, 947, 1161
ApoI RAATTY 2 cut(s) 401, 1512
ArsI GACNNNNNNTTYG 2 cut(s) 593, 625
AseI ATTAAT 1 cut(s) 225
Asp700I GAANNNNTTC 1 cut(s) 1231
AspLEI GCGC 1 cut(s) 809
AsuHPI GGTGA 1 cut(s) 806
AsuII TTCGAA 1 cut(s) 642
BanII GRGCYC 2 cut(s) 296, 1158
Bbv12I GWGCWC 3 cut(s) 296, 1158, 1191
BbvI GCAGC 3 cut(s) 494, 959, 1148
BccI CCATC 4 cut(s) 216, 538, 678, 914
BcgI CGANNNNNNTGC 2 cut(s) 622, 656
BciT130I CCWGG 3 cut(s) 88, 1459, 1534
BclI TGATCA 1 cut(s) 357
BcoDI GTCTC 3 cut(s) 428, 544, 1512
BfaI CTAG 5 cut(s) 491, 507, 743, 1008, 1419
BfmI CTRYAG 2 cut(s) 1162, 1502
BfrI CTTAAG 1 cut(s) 253
BglII AGATCT 1 cut(s) 979
BisI GCNGC 4 cut(s) 483, 948, 1072, 1162
BlsI GCNGC 4 cut(s) 484, 949, 1073, 1163
BmcAI AGTACT 1 cut(s) 1148
Bme1390I CCNGG 3 cut(s) 88, 1459, 1534
BmiI GGNNCC 1 cut(s) 897
BmrFI CCNGG 3 cut(s) 88, 1459, 1534
BmsI GCATC 5 cut(s) 115, 820, 994, 1246, 1543
BpmI CTGGAG 2 cut(s) 114, 741
Bpu10I CCTNAGC 1 cut(s) 790
Bpu14I TTCGAA 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 1217
Bsa29I ATCGAT 1 cut(s) 1551
BsaJI CCNNGG 4 cut(s) 87, 264, 1284, 1559
Bsc4I CCNNNNNNNGG 1 cut(s) 1198
Bse118I RCCGGY 1 cut(s) 1325
Bse1I ACTGG 3 cut(s) 845, 1221, 1428
BseBI CCWGG 3 cut(s) 88, 1459, 1534
BseCI ATCGAT 1 cut(s) 1551
BseDI CCNNGG 4 cut(s) 87, 264, 1284, 1559
BseGI GGATG 1 cut(s) 689
BseLI CCNNNNNNNGG 1 cut(s) 1198
BseMII CTCAG 5 cut(s) 258, 461, 512, 555, 665
BseNI ACTGG 3 cut(s) 845, 1221, 1428
BseRI GAGGAG 1 cut(s) 203
BseXI GCAGC 3 cut(s) 494, 959, 1148
BshFI GGCC 1 cut(s) 816
BshVI ATCGAT 1 cut(s) 1551
BsiHKAI GWGCWC 3 cut(s) 296, 1158, 1191
BsiSI CCGG 1 cut(s) 1326
BslFI GGGAC 2 cut(s) 1037, 1611
BslI CCNNNNNNNGG 1 cut(s) 1198
BsmAI GTCTC 3 cut(s) 428, 544, 1512
BsmFI GGGAC 2 cut(s) 1037, 1611
BsmI GAATGC 2 cut(s) 75, 809
BsnI GGCC 1 cut(s) 816
Bsp119I TTCGAA 1 cut(s) 642
Bsp1286I GDGCHC 3 cut(s) 296, 1158, 1191
Bsp1407I TGTACA 1 cut(s) 229
Bsp143I GATC 8 cut(s) 153, 172, 314, 357, 493, 779, 979, 1275
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 2 cut(s) 945, 1071
BspANI GGCC 1 cut(s) 816
BspCNI CTCAG 5 cut(s) 257, 460, 511, 554, 666
BspDI ATCGAT 1 cut(s) 1551
BspHI TCATGA 1 cut(s) 1183
BspLI GGNNCC 1 cut(s) 897
BspMAI CTGCAG 1 cut(s) 1166
BspPI GGATC 1 cut(s) 488
BspT104I TTCGAA 1 cut(s) 642
BspTI CTTAAG 1 cut(s) 253
BsrFI RCCGGY 1 cut(s) 1325
BsrGI TGTACA 1 cut(s) 229
BsrI ACTGG 3 cut(s) 845, 1221, 1428
BssAI RCCGGY 1 cut(s) 1325
BssECI CCNNGG 4 cut(s) 87, 264, 1284, 1559
BssMI GATC 8 cut(s) 153, 172, 314, 357, 493, 779, 979, 1275
BssT1I CCWWGG 3 cut(s) 264, 1284, 1559
Bst2UI CCWGG 3 cut(s) 88, 1459, 1534
Bst4CI ACNGT 2 cut(s) 11, 1506
Bst6I CTCTTC 2 cut(s) 1407, 1605
BstAFI CTTAAG 1 cut(s) 253
BstAUI TGTACA 1 cut(s) 229
BstBI TTCGAA 1 cut(s) 642
BstC8I GCNNGC 5 cut(s) 717, 1270, 1323, 1327, 1380
BstDEI CTNAG 8 cut(s) 244, 447, 498, 541, 674, 730, 756, 790
BstDSI CCRYGG 1 cut(s) 264
BstENI CCTNNNNNAGG 1 cut(s) 1196
BstF5I GGATG 1 cut(s) 689
BstHHI GCGC 1 cut(s) 809
BstKTI GATC 8 cut(s) 156, 175, 317, 360, 496, 782, 982, 1278
BstMAI GTCTC 3 cut(s) 428, 544, 1512
BstMBI GATC 8 cut(s) 153, 172, 314, 357, 493, 779, 979, 1275
BstMWI GCNNNNNNNGC 3 cut(s) 813, 1265, 1388
BstNI CCWGG 3 cut(s) 88, 1459, 1534
BstNSI RCATGY 2 cut(s) 539, 870
BstSCI CCNGG 3 cut(s) 86, 1457, 1532
BstSFI CTRYAG 2 cut(s) 1162, 1502
BstV1I GCAGC 3 cut(s) 494, 959, 1148
BstX2I RGATCY 2 cut(s) 493, 979
BstYI RGATCY 2 cut(s) 493, 979
Bsu15I ATCGAT 1 cut(s) 1551
BsuRI GGCC 1 cut(s) 816
BsuTUI ATCGAT 1 cut(s) 1551
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 1 cut(s) 689
BtsIMutI CAGTG 4 cut(s) 378, 912, 996, 1524
Cac8I GCNNGC 5 cut(s) 717, 1270, 1323, 1327, 1380
CciI TCATGA 1 cut(s) 1183
CfoI GCGC 1 cut(s) 809
Cfr10I RCCGGY 1 cut(s) 1325
ClaI ATCGAT 1 cut(s) 1551
CsiI ACCWGGT 1 cut(s) 1457
Csp6I GTAC 2 cut(s) 230, 1147
CviAII CATG 7 cut(s) 265, 511, 536, 867, 1184, 1342, 1629
CviQI GTAC 2 cut(s) 230, 1147
DdeI CTNAG 8 cut(s) 244, 447, 498, 541, 674, 730, 756, 790
DpnI GATC 8 cut(s) 155, 174, 316, 359, 495, 781, 981, 1277
DpnII GATC 8 cut(s) 153, 172, 314, 357, 493, 779, 979, 1275
Eam1104I CTCTTC 2 cut(s) 1407, 1605
EarI CTCTTC 2 cut(s) 1407, 1605
Ecl136II GAGCTC 2 cut(s) 294, 1156
Eco130I CCWWGG 3 cut(s) 264, 1284, 1559
Eco24I GRGCYC 2 cut(s) 296, 1158
Eco53kI GAGCTC 2 cut(s) 294, 1156
Eco57I CTGAAG 3 cut(s) 462, 1020, 1629
EcoICRI GAGCTC 2 cut(s) 294, 1156
EcoNI CCTNNNNNAGG 1 cut(s) 1196
EcoRII CCWGG 3 cut(s) 86, 1457, 1532
EcoT14I CCWWGG 3 cut(s) 264, 1284, 1559
EcoT22I ATGCAT 1 cut(s) 868
EcoT38I GRGCYC 2 cut(s) 296, 1158
ErhI CCWWGG 3 cut(s) 264, 1284, 1559
FaeI CATG 7 cut(s) 268, 514, 539, 870, 1187, 1345, 1632
FaqI GGGAC 2 cut(s) 1037, 1611
FatI CATG 7 cut(s) 264, 510, 535, 866, 1183, 1341, 1628
FbaI TGATCA 1 cut(s) 357
Fnu4HI GCNGC 4 cut(s) 483, 948, 1072, 1162
FokI GGATG 1 cut(s) 696
FriOI GRGCYC 2 cut(s) 296, 1158
Fsp4HI GCNGC 4 cut(s) 483, 948, 1072, 1162
FspBI CTAG 5 cut(s) 491, 507, 743, 1008, 1419
GlaI GCGC 1 cut(s) 808
GluI GCNGC 4 cut(s) 483, 948, 1072, 1162
GsuI CTGGAG 2 cut(s) 114, 741
HaeIII GGCC 1 cut(s) 816
HapII CCGG 1 cut(s) 1326
HhaI GCGC 1 cut(s) 809
Hin1II CATG 7 cut(s) 268, 514, 539, 870, 1187, 1345, 1632
Hin6I GCGC 1 cut(s) 807
HinP1I GCGC 1 cut(s) 807
HincII GTYRAC 1 cut(s) 1620
HindII GTYRAC 1 cut(s) 1620
HindIII AAGCTT 3 cut(s) 1034, 1469, 1523
HpaII CCGG 1 cut(s) 1326
HphI GGTGA 1 cut(s) 806
Hpy166II GTNNAC 3 cut(s) 232, 962, 1620
Hpy188III TCNNGA 7 cut(s) 131, 461, 543, 917, 1184, 1196, 1509
Hpy8I GTNNAC 3 cut(s) 232, 962, 1620
HpyAV CCTTC 6 cut(s) 109, 1019, 1145, 1190, 1202, 1486
HpyCH4III ACNGT 2 cut(s) 11, 1506
HpyCH4IV ACGT 2 cut(s) 964, 1406
HpyF10VI GCNNNNNNNGC 3 cut(s) 813, 1265, 1388
HpyF3I CTNAG 8 cut(s) 244, 447, 498, 541, 674, 730, 756, 790
HpySE526I ACGT 2 cut(s) 964, 1406
Hsp92II CATG 7 cut(s) 268, 514, 539, 870, 1187, 1345, 1632
HspAI GCGC 1 cut(s) 807
KroI GCCGGC 1 cut(s) 1325
KroNI GCCGGC 1 cut(s) 1327
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 8 cut(s) 153, 172, 314, 357, 493, 779, 979, 1275
LmnI GCTCC 4 cut(s) 133, 299, 1432, 1561
Lsp1109I GCAGC 3 cut(s) 494, 959, 1148
LweI GCATC 5 cut(s) 115, 820, 994, 1246, 1543
MabI ACCWGGT 1 cut(s) 1457
MaeI CTAG 5 cut(s) 491, 507, 743, 1008, 1419
MaeII ACGT 2 cut(s) 964, 1406
MaeIII GTNAC 1 cut(s) 745
MalI GATC 8 cut(s) 155, 174, 316, 359, 495, 781, 981, 1277
MboI GATC 8 cut(s) 153, 172, 314, 357, 493, 779, 979, 1275
MboII GAAGA 9 cut(s) 341, 431, 632, 1001, 1013, 1424, 1451, 1501, 1622
MfeI CAATTG 1 cut(s) 699
MflI RGATCY 2 cut(s) 493, 979
MhlI GDGCHC 3 cut(s) 296, 1158, 1191
MluCI AATT 6 cut(s) 401, 699, 824, 1230, 1397, 1512
MlyI GAGTC 4 cut(s) 257, 1052, 1393, 1593
MmeI TCCRAC 1 cut(s) 1554
MnlI CCTC 8 cut(s) 224, 300, 469, 507, 539, 683, 1192, 1408
Mph1103I ATGCAT 1 cut(s) 868
MroNI GCCGGC 1 cut(s) 1325
MroXI GAANNNNTTC 1 cut(s) 1231
MseI TTAA 8 cut(s) 225, 254, 405, 582, 1106, 1206, 1263, 1400
MspCI CTTAAG 1 cut(s) 253
MspI CCGG 1 cut(s) 1326
MspR9I CCNGG 3 cut(s) 88, 1459, 1534
MunI CAATTG 1 cut(s) 699
Mva1269I GAATGC 2 cut(s) 75, 809
MvaI CCWGG 3 cut(s) 88, 1459, 1534
MwoI GCNNNNNNNGC 3 cut(s) 813, 1265, 1388
NaeI GCCGGC 1 cut(s) 1327
NcoI CCATGG 1 cut(s) 264
NdeII GATC 8 cut(s) 153, 172, 314, 357, 493, 779, 979, 1275
NgoMIV GCCGGC 1 cut(s) 1325
NlaIII CATG 7 cut(s) 268, 514, 539, 870, 1187, 1345, 1632
NlaIV GGNNCC 1 cut(s) 897
NmeAIII GCCGAG 1 cut(s) 1583
NmuCI GTSAC 1 cut(s) 745
NsiI ATGCAT 1 cut(s) 868
NspI RCATGY 2 cut(s) 539, 870
NspV TTCGAA 1 cut(s) 642
PagI TCATGA 1 cut(s) 1183
PciI ACATGT 1 cut(s) 535
PctI GAATGC 2 cut(s) 75, 809
PdiI GCCGGC 1 cut(s) 1327
PdmI GAANNNNTTC 1 cut(s) 1231
PfeI GAWTC 6 cut(s) 64, 368, 549, 752, 851, 1029
PkrI GCNGC 4 cut(s) 484, 949, 1073, 1163
PleI GAGTC 4 cut(s) 256, 1051, 1392, 1593
PpsI GAGTC 4 cut(s) 256, 1051, 1392, 1593
PscI ACATGT 1 cut(s) 535
PshBI ATTAAT 1 cut(s) 225
Psp124BI GAGCTC 2 cut(s) 296, 1158
Psp6I CCWGG 3 cut(s) 86, 1457, 1532
PspGI CCWGG 3 cut(s) 86, 1457, 1532
PspN4I GGNNCC 1 cut(s) 897
PstI CTGCAG 1 cut(s) 1166
PsuI RGATCY 2 cut(s) 493, 979
RsaI GTAC 2 cut(s) 231, 1148
RsaNI GTAC 2 cut(s) 230, 1147
SacI GAGCTC 2 cut(s) 296, 1158
SaqAI TTAA 8 cut(s) 225, 254, 405, 582, 1106, 1206, 1263, 1400
SatI GCNGC 4 cut(s) 483, 948, 1072, 1162
Sau3AI GATC 8 cut(s) 153, 172, 314, 357, 493, 779, 979, 1275
ScaI AGTACT 1 cut(s) 1148
SchI GAGTC 4 cut(s) 257, 1052, 1393, 1593
ScrFI CCNGG 3 cut(s) 88, 1459, 1534
SduI GDGCHC 3 cut(s) 296, 1158, 1191
SexAI ACCWGGT 1 cut(s) 1457
SfaNI GCATC 5 cut(s) 115, 820, 994, 1246, 1543
SfcI CTRYAG 2 cut(s) 1162, 1502
SfuI TTCGAA 1 cut(s) 642
SmlI CTYRAG 2 cut(s) 253, 1196
SmoI CTYRAG 2 cut(s) 253, 1196
Sse9I AATT 6 cut(s) 401, 699, 824, 1230, 1397, 1512
SsiI CCGC 2 cut(s) 945, 1071
SspI AATATT 1 cut(s) 1249
SspMI CTAG 5 cut(s) 491, 507, 743, 1008, 1419
SstI GAGCTC 2 cut(s) 296, 1158
StyD4I CCNGG 3 cut(s) 86, 1457, 1532
StyI CCWWGG 3 cut(s) 264, 1284, 1559
TaaI ACNGT 2 cut(s) 11, 1506
TaiI ACGT 2 cut(s) 967, 1409
TaqI TCGA 6 cut(s) 123, 462, 642, 841, 1510, 1551
TasI AATT 6 cut(s) 401, 699, 824, 1230, 1397, 1512
TatI WGTACW 2 cut(s) 229, 1146
TauI GCSGC 1 cut(s) 1074
TfiI GAWTC 6 cut(s) 64, 368, 549, 752, 851, 1029
Tru1I TTAA 8 cut(s) 225, 254, 405, 582, 1106, 1206, 1263, 1400
Tru9I TTAA 8 cut(s) 225, 254, 405, 582, 1106, 1206, 1263, 1400
TscAI CASTG 4 cut(s) 378, 912, 1003, 1524
TseFI GTSAC 1 cut(s) 745
TseI GCWGC 3 cut(s) 482, 947, 1161
Tsp45I GTSAC 1 cut(s) 745
TspDTI ATGAA 6 cut(s) 342, 639, 728, 1077, 1172, 1358
TspRI CASTG 4 cut(s) 378, 912, 1003, 1524
Vha464I CTTAAG 1 cut(s) 253
VspI ATTAAT 1 cut(s) 225
XagI CCTNNNNNAGG 1 cut(s) 1196
XapI RAATTY 2 cut(s) 401, 1512
XceI RCATGY 2 cut(s) 539, 870
XmnI GAANNNNTTC 1 cut(s) 1231
XspI CTAG 5 cut(s) 491, 507, 743, 1008, 1419
ZrmI AGTACT 1 cut(s) 1148
Zsp2I ATGCAT 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.