Rorug05G0577900

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004841
Physical Location & Seq
Forward (+)
77398835 .. 77401883
3049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0577900.1

Sequence Viewer

Length: 2232 bp
ATGACAAAAGGGTTTACTGTGTTTTCTAGTTTTCCGGCGACTCATGTGATTTGGCTGTCTACCTTTCCACCTCAAATTACCGACACGCGATCTTCACCATCACCGGAGTCCAGTGCAATTTCACAGTTTGCTTTCTCAGTGATCAAGTTCCGTAAAAGTGCGGAATCACAACCCCGTGTAAATAGCATATACATTATGATGACAGGAAACAACAACAAAGATGTTGCAGAATCATCAGAAGTTCTGAATAGAAGCTCTAATGACATTGAACAGAAGAGAAACTCGCACGAAAACAAACAGCAGAGTAATTTTCATGAAATTCAATTGAAGAAACACTCTCATGATAGTACTGAAGAGAAAAGGGACTCACATGATACTGAAGAGAATAGGAACTCTCACGGTACTGAAGAGAAAACCAACTCCAACTCTCATGATATTGAAGAGAAGACCAACTCTCATGAAAATGAAGGGAAGATCGACTCCCATGAAGGTGATGAACCACCTGAGAAAGGGACAATCACTACTAGAAGTCCCAATGCACCAAGCTCTATTAGAAAGAGAAGAGGAGGATGGAAACTTGCAATTCTCTTGCTAGCAAATCAAGGCCTAGCAACACTAGCTTTCTTTGGTGTTGGTGTGAACTTGGTTTTGTTTCTAGTCAGAGTTCTTGAACAATCAAATGCCGTAGCGGCCAACAATGTTAGCAAGTGGACTGGAACAGTTTATCTGTTCTCTCTGGTTGGAGCTTTCCTCAGCGATTCCTATTGGGGCCGTTACTTAACCTGTGCCGTCTTTCAGGTCTTCTTTGTAATGGGCTTGGGGCTAATGTCACTTACATCTTGGCTCTTTCTGATCCATCCATCTGGTTGTGGCGATGGAGAAAAACTTTGCAATCCCCCATCCAAAGCTGGTGTTATTCTTTTTTACCTATCAATATATTTAGTGGCATTTGGATATGGTGGTCACCAACCTACTATAGCAACATTTGGAGCGGACCAATTTGATGAGGCAGATCCTGAACAGCAAAGTTCCAGAGCAGTTTTCTTCTCTTATTTCTACTTTGCACTCAACGTGGGGTGTCTCTTCTCAAACACCATCTTAGTTTATTATGAGGATCATGGATATTGGAATATAGGGTTTCTGGTCTCATTAGGGTCAGCAGTCATAGCCCTATTGTCCTATTTGATGGGATCAAGTCGGTATCGGTATATAAAACCATGTGGCAACCCTTTACCTCGTGTTGCTCAGGTATTTGTGGCTGCTGCTAGAAAATGGCATGTTGTTCCAGCTGATCCAGATAATCTGTATGAGATGGAAGGAAAAGAATCTGCAATCAAAGGGAGTAGAAAGATTCTTCACAGTAATGAAATTGAGTTTTTGGACAAGGCAGCAACGATAACGGACAGGGATCAAAATGAGAAAAACCCTTGGAGACTTTGTACTGTAACCCAAGTCGAAGAAGCCAAATGCGTCCTGAAACTTCTACCTATTTGGCTTTGCACTATTATTTACTCAGTGATCTTCACACAAATGGCTTCTCTATTCGTAGAGCAAGGTGATGTGATGCATGCCCAAATCGGAAATTTTCATATCCCAGCTGCTAGCATGTCAGCTTTTGACATATGCAGCGTCCTAATCTCCACCGGAATTTACCGCCAAGTCCTTGTGCCTTTAGCTGGAAGATTAAGTGGAAACCCTAAAGGGTTATCAGAGCTTCAGAGAATGGGAATTGGACTAATTATTGGAATGTTAGCAATGGTTGCAGCTGGTGCCACGGAGATTGCAAGGCTCCAATATGTGGAGCCGGGTAAGAAAGTAAGCTCTTTGAGCATATTTTGGCAAATTCCACAATATGTTTTTGTTGGTGTTTCAGAAGTTTTCATGTATGTTGGTCAACTTGAGTTCTTTAATGGAGAAGCCCCAGATGGGATAAAAAGCTTTGGAAGTTCGTTGTGCATGGCTTCAATCTCTCTTGGGAATTATGTGAGCAGTATGCTAGTTGCTATGGTGATGGGGATTACAGCTAGAGGTTCAGACCCAGGTTGGATTCCAAATAATTTGAATGAAGGTCATATGGACAGGTTCTACTTCTTGATTGCAGGACTAACAGTTCTCGACTTTGCTTTTTATTTATATCTTGCTAAGAGGTACAAGTGCATTAACCTTGAAGGCCCTGAGAATGAAAAGGTTAGTCAAGAAAGTCAGGATGATGAGGTGCTCAGAAGCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

743

Amino Acids

82.49

Weight (kDa)

5.95

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 261 - 682 1.2e-88 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1769
AccB7I CCANNNNNTGG 1 cut(s) 1798
AccBSI CCGCTC 1 cut(s) 992
AccI GTMKAC 1 cut(s) 59
AccII CGCG 1 cut(s) 88
AciI CCGC 4 cut(s) 161, 689, 992, 1654
AclWI GGATC 6 cut(s) 847, 1007, 1122, 1198, 1286, 1416
AcoI YGGCCR 1 cut(s) 690
AcsI RAATTY 4 cut(s) 318, 1582, 1647, 1842
AcuI CTGAAG 4 cut(s) 372, 399, 426, 1700
AfaI GTAC 4 cut(s) 349, 403, 1441, 2150
AfiI CCNNNNNNNGG 4 cut(s) 509, 1676, 1798, 1926
AgsI TTSAA 8 cut(s) 269, 323, 328, 440, 671, 1965, 2062, 2168
AjnI CCWGG 1 cut(s) 2038
AloI GAACNNNNNNTCC 2 cut(s) 2094, 2126
Alw21I GWGCWC 1 cut(s) 2220
Alw26I GTCTC 3 cut(s) 1085, 1150, 1426
AlwI GGATC 6 cut(s) 847, 1007, 1122, 1198, 1286, 1416
AlwNI CAGNNNCTG 1 cut(s) 1016
AoxI GGCC 4 cut(s) 604, 690, 769, 2170
ApeKI GCWGC 6 cut(s) 1259, 1262, 1388, 1598, 1626, 1763
ApoI RAATTY 4 cut(s) 318, 1582, 1647, 1842
AspS9I GGNCC 3 cut(s) 769, 994, 2171
AsuC2I CCSGG 1 cut(s) 1806
AsuHPI GGTGA 6 cut(s) 87, 93, 503, 956, 1568, 2020
AsuNHI GCTAGC 2 cut(s) 592, 1601
AvaII GGWCC 1 cut(s) 994
BanI GGYRCC 1 cut(s) 1769
BauI CACGAG 1 cut(s) 1236
BbsI GAAGAC 2 cut(s) 452, 793
Bbv12I GWGCWC 1 cut(s) 2220
BbvCI CCTCAGC 1 cut(s) 752
BbvI GCAGC 6 cut(s) 1246, 1249, 1400, 1585, 1638, 1775
BceAI ACGGC 3 cut(s) 668, 756, 773
BciT130I CCWGG 1 cut(s) 2040
BclI TGATCA 1 cut(s) 141
BcnI CCSGG 1 cut(s) 1806
BcoDI GTCTC 3 cut(s) 1085, 1150, 1426
BfmI CTRYAG 1 cut(s) 975
BglI GCCNNNNNGGC 1 cut(s) 689
BisI GCNGC 7 cut(s) 690, 1260, 1263, 1389, 1599, 1627, 1764
BlsI GCNGC 7 cut(s) 691, 1261, 1264, 1390, 1600, 1628, 1765
BmcAI AGTACT 1 cut(s) 349
Bme1390I CCNGG 2 cut(s) 1806, 2040
Bme18I GGWCC 1 cut(s) 994
BmgT120I GGNCC 3 cut(s) 769, 994, 2171
BmiI GGNNCC 4 cut(s) 770, 1771, 1790, 1803
BmrFI CCNGG 2 cut(s) 1806, 2040
BmsI GCATC 1 cut(s) 1554
BmtI GCTAGC 2 cut(s) 596, 1605
BpiI GAAGAC 2 cut(s) 452, 793
BplI GAGNNNNNCTC 2 cut(s) 735, 767
Bpu10I CCTNAGC 2 cut(s) 752, 1245
BpuEI CTTGAG 1 cut(s) 1919
BpuMI CCSGG 1 cut(s) 1806
BsaI GGTCTC 1 cut(s) 1150
BsaJI CCNNGG 3 cut(s) 1427, 1773, 2038
BsaWI WCCGGW 2 cut(s) 103, 1643
BsaXI ACNNNNNCTCC 2 cut(s) 1423, 1453
Bsc4I CCNNNNNNNGG 4 cut(s) 509, 1676, 1798, 1926
Bse1I ACTGG 2 cut(s) 111, 718
Bse3DI GCAATG 1 cut(s) 1761
BseBI CCWGG 1 cut(s) 2040
BseDI CCNNGG 3 cut(s) 1427, 1773, 2038
BseGI GGATG 4 cut(s) 575, 856, 899, 2212
BseLI CCNNNNNNNGG 4 cut(s) 509, 1676, 1798, 1926
BseMI GCAATG 1 cut(s) 1761
BseMII CTCAG 6 cut(s) 150, 495, 766, 1259, 1527, 2166
BseNI ACTGG 2 cut(s) 111, 718
BseRI GAGGAG 1 cut(s) 579
BseXI GCAGC 6 cut(s) 1246, 1249, 1400, 1585, 1638, 1775
BseYI CCCAGC 1 cut(s) 1594
Bsh1236I CGCG 1 cut(s) 88
BshFI GGCC 4 cut(s) 606, 692, 771, 2172
BshNI GGYRCC 1 cut(s) 1769
BsiHKAI GWGCWC 1 cut(s) 2220
BsiSI CCGG 4 cut(s) 35, 104, 1644, 1805
BslFI GGGAC 3 cut(s) 377, 516, 526
BslI CCNNNNNNNGG 4 cut(s) 509, 1676, 1798, 1926
BsmAI GTCTC 3 cut(s) 1085, 1150, 1426
BsmFI GGGAC 3 cut(s) 377, 516, 526
BsnI GGCC 4 cut(s) 606, 692, 771, 2172
Bso31I GGTCTC 1 cut(s) 1150
Bsp1286I GDGCHC 1 cut(s) 2220
BspACI CCGC 4 cut(s) 161, 689, 992, 1654
BspANI GGCC 4 cut(s) 606, 692, 771, 2172
BspCNI CTCAG 7 cut(s) 149, 496, 765, 1258, 1526, 2167, 2232
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 4 cut(s) 313, 340, 430, 457
BspLI GGNNCC 4 cut(s) 770, 1771, 1790, 1803
BspOI GCTAGC 2 cut(s) 596, 1605
BspPI GGATC 6 cut(s) 847, 1007, 1122, 1198, 1286, 1416
BspT107I GGYRCC 1 cut(s) 1769
BspTNI GGTCTC 1 cut(s) 1150
BsrBI CCGCTC 1 cut(s) 992
BsrDI GCAATG 1 cut(s) 1761
BsrI ACTGG 2 cut(s) 111, 718
BssECI CCNNGG 3 cut(s) 1427, 1773, 2038
BssSI CACGAG 1 cut(s) 1236
BssT1I CCWWGG 1 cut(s) 1427
Bst2BI CACGAG 1 cut(s) 1236
Bst2UI CCWGG 1 cut(s) 2040
Bst4CI ACNGT 7 cut(s) 19, 126, 401, 721, 1361, 1444, 2110
Bst6I CTCTTC 7 cut(s) 269, 348, 375, 402, 435, 556, 1088
BstAPI GCANNNNNTGC 2 cut(s) 1760, 1769
BstC8I GCNNGC 3 cut(s) 594, 1569, 1603
BstDEI CTNAG 9 cut(s) 136, 504, 752, 1099, 1245, 1513, 2142, 2175, 2219
BstDSI CCRYGG 1 cut(s) 1773
BstEII GGTNACC 1 cut(s) 962
BstENI CCTNNNNNAGG 1 cut(s) 507
BstF5I GGATG 4 cut(s) 575, 856, 899, 2212
BstFNI CGCG 1 cut(s) 88
BstMAI GTCTC 3 cut(s) 1085, 1150, 1426
BstMWI GCNNNNNNNGC 6 cut(s) 617, 689, 1166, 1760, 1769, 1827
BstNI CCWGG 1 cut(s) 2040
BstNSI RCATGY 3 cut(s) 1280, 1571, 1609
BstPI GGTNACC 1 cut(s) 962
BstSCI CCNGG 2 cut(s) 1804, 2038
BstSFI CTRYAG 1 cut(s) 975
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 6 cut(s) 1246, 1249, 1400, 1585, 1638, 1775
BstV2I GAAGAC 2 cut(s) 452, 793
BstX2I RGATCY 1 cut(s) 1012
BstXI CCANNNNNNTGG 1 cut(s) 863
BstYI RGATCY 1 cut(s) 1012
BsuRI GGCC 4 cut(s) 606, 692, 771, 2172
BtgI CCRYGG 1 cut(s) 1773
BtgZI GCGATG 1 cut(s) 888
BtsCI GGATG 4 cut(s) 575, 856, 899, 2212
BtsIMutI CAGTG 3 cut(s) 118, 144, 1521
Cac8I GCNNGC 3 cut(s) 594, 1569, 1603
CaiI CAGNNNCTG 1 cut(s) 1016
CciI TCATGA 4 cut(s) 313, 340, 430, 457
Cfr13I GGNCC 3 cut(s) 769, 994, 2171
CseI GACGC 2 cut(s) 1459, 1618
Csp6I GTAC 4 cut(s) 348, 402, 1440, 2149
CspCI CAANNNNNGTGG 2 cut(s) 1762, 1797
CviQI GTAC 4 cut(s) 348, 402, 1440, 2149
DdeI CTNAG 9 cut(s) 136, 504, 752, 1099, 1245, 1513, 2142, 2175, 2219
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 7 cut(s) 269, 348, 375, 402, 435, 556, 1088
EarI CTCTTC 7 cut(s) 269, 348, 375, 402, 435, 556, 1088
Eco130I CCWWGG 1 cut(s) 1427
Eco147I AGGCCT 1 cut(s) 606
Eco31I GGTCTC 1 cut(s) 1150
Eco47I GGWCC 1 cut(s) 994
Eco57I CTGAAG 4 cut(s) 372, 399, 426, 1700
Eco91I GGTNACC 1 cut(s) 962
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoO109I RGGNCCY 1 cut(s) 2171
EcoO65I GGTNACC 1 cut(s) 962
EcoRII CCWGG 1 cut(s) 2038
EcoT14I CCWWGG 1 cut(s) 1427
EcoT22I ATGCAT 1 cut(s) 1569
ErhI CCWWGG 1 cut(s) 1427
FaqI GGGAC 3 cut(s) 377, 516, 526
FauNDI CATATG 2 cut(s) 1622, 2073
FbaI TGATCA 1 cut(s) 141
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 7 cut(s) 690, 1260, 1263, 1389, 1599, 1627, 1764
FokI GGATG 4 cut(s) 582, 843, 886, 2219
Fsp4HI GCNGC 7 cut(s) 690, 1260, 1263, 1389, 1599, 1627, 1764
GluI GCNGC 7 cut(s) 690, 1260, 1263, 1389, 1599, 1627, 1764
GsaI CCCAGC 1 cut(s) 1598
HaeIII GGCC 4 cut(s) 606, 692, 771, 2172
HapII CCGG 4 cut(s) 35, 104, 1644, 1805
HgaI GACGC 2 cut(s) 1459, 1618
HincII GTYRAC 1 cut(s) 1895
HindII GTYRAC 1 cut(s) 1895
HindIII AAGCTT 1 cut(s) 1936
HpaII CCGG 4 cut(s) 35, 104, 1644, 1805
HphI GGTGA 6 cut(s) 87, 93, 503, 956, 1568, 2020
Hpy166II GTNNAC 5 cut(s) 15, 60, 640, 711, 1895
Hpy8I GTNNAC 5 cut(s) 15, 60, 640, 711, 1895
HpyAV CCTTC 5 cut(s) 461, 482, 1310, 2060, 2162
HpyCH4III ACNGT 7 cut(s) 19, 126, 401, 721, 1361, 1444, 2110
HpyCH4IV ACGT 1 cut(s) 1071
HpyF10VI GCNNNNNNNGC 6 cut(s) 617, 689, 1166, 1760, 1769, 1827
HpyF3I CTNAG 9 cut(s) 136, 504, 752, 1099, 1245, 1513, 2142, 2175, 2219
HpySE526I ACGT 1 cut(s) 1071
Ksp22I TGATCA 1 cut(s) 141
LmnI GCTCC 4 cut(s) 743, 989, 1794, 1801
Lsp1109I GCAGC 6 cut(s) 1246, 1249, 1400, 1585, 1638, 1775
LweI GCATC 1 cut(s) 1554
MaeII ACGT 1 cut(s) 1071
MaeIII GTNAC 4 cut(s) 773, 828, 962, 1444
MbiI CCGCTC 1 cut(s) 992
MfeI CAATTG 1 cut(s) 323
MflI RGATCY 1 cut(s) 1012
MhlI GDGCHC 1 cut(s) 2220
MlyI GAGTC 4 cut(s) 34, 116, 359, 473
MmeI TCCRAC 3 cut(s) 447, 721, 2024
Mph1103I ATGCAT 1 cut(s) 1569
MseI TTAA 5 cut(s) 779, 1685, 1908, 2160, 2230
MslI CAYNNNNRTG 6 cut(s) 197, 339, 462, 489, 1362, 1529
MspA1I CMGCKG 3 cut(s) 1289, 1598, 1766
MspI CCGG 4 cut(s) 35, 104, 1644, 1805
MspR9I CCNGG 2 cut(s) 1806, 2040
MunI CAATTG 1 cut(s) 323
MvaI CCWGG 1 cut(s) 2040
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 6 cut(s) 617, 689, 1166, 1760, 1769, 1827
NciI CCSGG 1 cut(s) 1806
NdeI CATATG 2 cut(s) 1622, 2073
NheI GCTAGC 2 cut(s) 592, 1601
NlaIV GGNNCC 4 cut(s) 770, 1771, 1790, 1803
NmuCI GTSAC 2 cut(s) 828, 962
NsiI ATGCAT 1 cut(s) 1569
NspI RCATGY 3 cut(s) 1280, 1571, 1609
PaeI GCATGC 1 cut(s) 1571
PagI TCATGA 4 cut(s) 313, 340, 430, 457
PceI AGGCCT 1 cut(s) 606
PfeI GAWTC 6 cut(s) 164, 230, 758, 1325, 1351, 2047
PflMI CCANNNNNTGG 1 cut(s) 1798
PkrI GCNGC 7 cut(s) 691, 1261, 1264, 1390, 1600, 1628, 1765
PleI GAGTC 4 cut(s) 34, 115, 359, 473
PpsI GAGTC 4 cut(s) 34, 115, 359, 473
Psp6I CCWGG 1 cut(s) 2038
PspEI GGTNACC 1 cut(s) 962
PspFI CCCAGC 1 cut(s) 1594
PspGI CCWGG 1 cut(s) 2038
PspN4I GGNNCC 4 cut(s) 770, 1771, 1790, 1803
PspPI GGNCC 3 cut(s) 769, 994, 2171
PstNI CAGNNNCTG 1 cut(s) 1016
PsuI RGATCY 1 cut(s) 1012
PvuII CAGCTG 3 cut(s) 1289, 1598, 1766
RsaI GTAC 4 cut(s) 349, 403, 1441, 2150
RsaNI GTAC 4 cut(s) 348, 402, 1440, 2149
RseI CAYNNNNRTG 6 cut(s) 197, 339, 462, 489, 1362, 1529
SaqAI TTAA 5 cut(s) 779, 1685, 1908, 2160, 2230
SatI GCNGC 7 cut(s) 690, 1260, 1263, 1389, 1599, 1627, 1764
Sau96I GGNCC 3 cut(s) 769, 994, 2171
ScaI AGTACT 1 cut(s) 349
SchI GAGTC 4 cut(s) 34, 116, 359, 473
ScrFI CCNGG 2 cut(s) 1806, 2040
SduI GDGCHC 1 cut(s) 2220
SfaNI GCATC 1 cut(s) 1554
SfcI CTRYAG 1 cut(s) 975
SinI GGWCC 1 cut(s) 994
SmiMI CAYNNNNRTG 6 cut(s) 197, 339, 462, 489, 1362, 1529
SmlI CTYRAG 1 cut(s) 1898
SmoI CTYRAG 1 cut(s) 1898
SphI GCATGC 1 cut(s) 1571
SseBI AGGCCT 1 cut(s) 606
SsiI CCGC 4 cut(s) 161, 689, 992, 1654
StuI AGGCCT 1 cut(s) 606
StyD4I CCNGG 2 cut(s) 1804, 2038
StyI CCWWGG 1 cut(s) 1427
TaaI ACNGT 7 cut(s) 19, 126, 401, 721, 1361, 1444, 2110
TaiI ACGT 1 cut(s) 1074
TaqI TCGA 3 cut(s) 477, 1455, 2115
TatI WGTACW 2 cut(s) 347, 1439
TauI GCSGC 1 cut(s) 692
TfiI GAWTC 6 cut(s) 164, 230, 758, 1325, 1351, 2047
Tru1I TTAA 5 cut(s) 779, 1685, 1908, 2160, 2230
Tru9I TTAA 5 cut(s) 779, 1685, 1908, 2160, 2230
TscAI CASTG 3 cut(s) 118, 144, 1521
TseFI GTSAC 2 cut(s) 828, 962
TseI GCWGC 6 cut(s) 1259, 1262, 1388, 1598, 1626, 1763
Tsp45I GTSAC 2 cut(s) 828, 962
TspGWI ACGGA 3 cut(s) 140, 1415, 1790
TspRI CASTG 3 cut(s) 118, 144, 1521
Van91I CCANNNNNTGG 1 cut(s) 1798
VpaK11BI GGWCC 1 cut(s) 994
XagI CCTNNNNNAGG 1 cut(s) 507
XapI RAATTY 4 cut(s) 318, 1582, 1647, 1842
XceI RCATGY 3 cut(s) 1280, 1571, 1609
XmiI GTMKAC 1 cut(s) 59
ZrmI AGTACT 1 cut(s) 349
Zsp2I ATGCAT 1 cut(s) 1569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.