RLG00000016386

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008185
Physical Location & Seq
Reverse (-)
6116097 .. 6116642
546 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016386

Sequence Viewer

Length: 432 bp
ATGCCGCTCCCAAAACCTAGCCGCCTATGCTTGGTGCTCCAAACATGGCGACCGCTGCCGGTTTCTGATCCTTGTCGAATTGATTTCGATCCAAACCGTGGCGGACCTCCAAGGGCAAGCTTCATGCGCTTTCCTCCAAGAGGCGGTGGCGGCTTGCTCTTAGGAGCGATTAGAGTCTCTGGCAATCGACTGTGTGGAGATCCTTCATCCATTGCTTCTCTTGAGTTGGGTCTTCGATCTACTTCATATGGGTGCTCGGTAGGAGGCTTTTCTCGGCCTCACCCTGTATTGGTCAAATTCTGGGATCTGAGGAAGTTGGTGAAATTGGTGATTCATCGCCGAAGCAAGCTTGTAGTGGAAGACGAGCTTGGTCGAACTCGACCGCGTAGGGGGCGTCTCAAACTCAGAGTTTTTGGTGACTCGAGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

15.9

Weight (kDa)

11.52

Isoelectric Point (pI)

55.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 98
AccBSI CCGCTC 1 cut(s) 7
AccII CGCG 1 cut(s) 385
AciI CCGC 7 cut(s) 5, 22, 53, 102, 144, 150, 383
AclWI GGATC 4 cut(s) 62, 83, 194, 312
AcsI RAATTY 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 394
AfiI CCNNNNNNNGG 6 cut(s) 31, 98, 140, 143, 289, 389
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
AluBI AGCT 3 cut(s) 120, 349, 367
AluI AGCT 3 cut(s) 120, 349, 367
Alw21I GWGCWC 2 cut(s) 39, 257
Alw26I GTCTC 2 cut(s) 181, 401
AlwI GGATC 4 cut(s) 62, 83, 194, 312
Ama87I CYCGRG 1 cut(s) 421
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 55
ApoI RAATTY 1 cut(s) 296
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 104
AsuHPI GGTGA 4 cut(s) 272, 331, 340, 428
AvaI CYCGRG 1 cut(s) 421
AvaII GGWCC 1 cut(s) 104
BbsI GAAGAC 2 cut(s) 224, 366
Bbv12I GWGCWC 2 cut(s) 39, 257
BbvI GCAGC 1 cut(s) 42
BcoDI GTCTC 2 cut(s) 181, 401
BfaI CTAG 1 cut(s) 18
BisI GCNGC 4 cut(s) 5, 22, 56, 151
BlsI GCNGC 4 cut(s) 6, 23, 57, 152
Bme18I GGWCC 1 cut(s) 104
BmeT110I CYCGRG 1 cut(s) 421
BmgT120I GGNCC 1 cut(s) 104
BpiI GAAGAC 2 cut(s) 224, 366
BpuEI CTTGAG 1 cut(s) 242
BsaBI GATNNNNATC 1 cut(s) 87
BsaHI GRCGYC 1 cut(s) 394
BsaJI CCNNGG 2 cut(s) 97, 110
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 98, 140, 143, 289, 389
Bse118I RCCGGY 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 210
Bse8I GATNNNNATC 1 cut(s) 87
BseDI CCNNGG 2 cut(s) 97, 110
BseGI GGATG 1 cut(s) 206
BseJI GATNNNNATC 1 cut(s) 87
BseLI CCNNNNNNNGG 6 cut(s) 31, 98, 140, 143, 289, 389
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 2 cut(s) 299, 418
BseXI GCAGC 1 cut(s) 42
Bsh1236I CGCG 1 cut(s) 385
Bsh1285I CGRYCG 2 cut(s) 53, 383
BshFI GGCC 1 cut(s) 277
BsiEI CGRYCG 2 cut(s) 53, 383
BsiHKAI GWGCWC 2 cut(s) 39, 257
BsiHKCI CYCGRG 1 cut(s) 421
BsiSI CCGG 1 cut(s) 59
BslI CCNNNNNNNGG 6 cut(s) 31, 98, 140, 143, 289, 389
BsmAI GTCTC 2 cut(s) 181, 401
BsmBI CGTCTC 1 cut(s) 401
BsnI GGCC 1 cut(s) 277
BsoBI CYCGRG 1 cut(s) 421
Bsp1286I GDGCHC 2 cut(s) 39, 257
Bsp143I GATC 5 cut(s) 67, 88, 199, 236, 304
BspACI CCGC 7 cut(s) 5, 22, 53, 102, 144, 150, 383
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 2 cut(s) 300, 417
BspFNI CGCG 1 cut(s) 385
BspPI GGATC 4 cut(s) 62, 83, 194, 312
BsrBI CCGCTC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 58
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 2 cut(s) 97, 110
BssMI GATC 5 cut(s) 67, 88, 199, 236, 304
BssNI GRCGYC 1 cut(s) 394
BssT1I CCWWGG 1 cut(s) 110
Bst4CI ACNGT 2 cut(s) 98, 192
BstACI GRCGYC 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 118, 155, 347
BstDEI CTNAG 3 cut(s) 160, 308, 404
BstDSI CCRYGG 1 cut(s) 97
BstENI CCTNNNNNAGG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 206
BstFNI CGCG 1 cut(s) 385
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 5 cut(s) 70, 91, 202, 239, 307
BstMAI GTCTC 2 cut(s) 181, 401
BstMBI GATC 5 cut(s) 67, 88, 199, 236, 304
BstMCI CGRYCG 2 cut(s) 53, 383
BstMWI GCNNNNNNNGC 5 cut(s) 27, 55, 126, 150, 391
BstUI CGCG 1 cut(s) 385
BstV1I GCAGC 1 cut(s) 42
BstV2I GAAGAC 2 cut(s) 224, 366
BstX2I RGATCY 2 cut(s) 199, 304
BstYI RGATCY 2 cut(s) 199, 304
BsuRI GGCC 1 cut(s) 277
BtgI CCRYGG 1 cut(s) 97
BtgZI GCGATG 1 cut(s) 320
BtsCI GGATG 1 cut(s) 206
Cac8I GCNNGC 3 cut(s) 118, 155, 347
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 1 cut(s) 104
CseI GACGC 1 cut(s) 383
CviAII CATG 2 cut(s) 45, 124
CviJI RGCY 7 cut(s) 21, 120, 153, 267, 277, 349, 367
CviKI_1 RGCY 7 cut(s) 21, 120, 153, 267, 277, 349, 367
DdeI CTNAG 3 cut(s) 160, 308, 404
DpnI GATC 5 cut(s) 69, 90, 201, 238, 306
DpnII GATC 5 cut(s) 67, 88, 199, 236, 304
EciI GGCGGA 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 104
Eco88I CYCGRG 1 cut(s) 421
EcoNI CCTNNNNNAGG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 110
Esp3I CGTCTC 1 cut(s) 401
FaeI CATG 2 cut(s) 48, 127
FaiI YATR 5 cut(s) 28, 46, 125, 247, 249
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FatI CATG 2 cut(s) 44, 123
FauNDI CATATG 1 cut(s) 247
Fnu4HI GCNGC 4 cut(s) 5, 22, 56, 151
FokI GGATG 1 cut(s) 193
Fsp4HI GCNGC 4 cut(s) 5, 22, 56, 151
FspBI CTAG 1 cut(s) 18
GlaI GCGC 1 cut(s) 128
GluI GCNGC 4 cut(s) 5, 22, 56, 151
HaeIII GGCC 1 cut(s) 277
HapII CCGG 1 cut(s) 59
HgaI GACGC 1 cut(s) 383
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 394
Hin1II CATG 2 cut(s) 48, 127
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HindIII AAGCTT 2 cut(s) 118, 347
HinfI GANTC 3 cut(s) 174, 331, 419
HpaII CCGG 1 cut(s) 59
HphI GGTGA 4 cut(s) 272, 331, 340, 428
Hpy188I TCNGA 3 cut(s) 67, 309, 407
Hpy188III TCNNGA 2 cut(s) 221, 423
HpyAV CCTTC 1 cut(s) 213
HpyCH4III ACNGT 2 cut(s) 98, 192
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 55, 126, 150, 391
HpyF3I CTNAG 3 cut(s) 160, 308, 404
Hsp92I GRCGYC 1 cut(s) 394
Hsp92II CATG 2 cut(s) 48, 127
HspAI GCGC 1 cut(s) 127
Kzo9I GATC 5 cut(s) 67, 88, 199, 236, 304
LmnI GCTCC 3 cut(s) 12, 42, 164
LpnPI CCDG 4 cut(s) 72, 165, 286, 297
Lsp1109I GCAGC 1 cut(s) 42
MaeI CTAG 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 416
MalI GATC 5 cut(s) 69, 90, 201, 238, 306
MbiI CCGCTC 1 cut(s) 7
MboI GATC 5 cut(s) 67, 88, 199, 236, 304
MboII GAAGA 2 cut(s) 224, 371
MflI RGATCY 2 cut(s) 199, 304
MhlI GDGCHC 2 cut(s) 39, 257
MluCI AATT 3 cut(s) 78, 296, 323
MlyI GAGTC 2 cut(s) 183, 413
MnlI CCTC 6 cut(s) 117, 134, 144, 257, 288, 303
MslI CAYNNNNRTG 1 cut(s) 250
MspA1I CMGCKG 1 cut(s) 55
MspI CCGG 1 cut(s) 59
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 5 cut(s) 27, 55, 126, 150, 391
NdeI CATATG 1 cut(s) 247
NdeII GATC 5 cut(s) 67, 88, 199, 236, 304
NlaIII CATG 2 cut(s) 48, 127
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 1 cut(s) 416
PaeR7I CTCGAG 1 cut(s) 421
PfeI GAWTC 1 cut(s) 331
PflMI CCANNNNNTGG 1 cut(s) 98
PkrI GCNGC 4 cut(s) 6, 23, 57, 152
PleI GAGTC 2 cut(s) 182, 413
PpsI GAGTC 2 cut(s) 182, 413
PspPI GGNCC 1 cut(s) 104
PsuI RGATCY 2 cut(s) 199, 304
RseI CAYNNNNRTG 1 cut(s) 250
SatI GCNGC 4 cut(s) 5, 22, 56, 151
Sau3AI GATC 5 cut(s) 67, 88, 199, 236, 304
Sau96I GGNCC 1 cut(s) 104
SchI GAGTC 2 cut(s) 183, 413
SduI GDGCHC 2 cut(s) 39, 257
SetI ASST 5 cut(s) 19, 109, 122, 351, 369
Sfr274I CTCGAG 1 cut(s) 421
SinI GGWCC 1 cut(s) 104
SlaI CTCGAG 1 cut(s) 421
SmiMI CAYNNNNRTG 1 cut(s) 250
SmlI CTYRAG 2 cut(s) 221, 421
SmoI CTYRAG 2 cut(s) 221, 421
Sse9I AATT 3 cut(s) 78, 296, 323
SsiI CCGC 7 cut(s) 5, 22, 53, 102, 144, 150, 383
SspMI CTAG 1 cut(s) 18
StyI CCWWGG 1 cut(s) 110
TaaI ACNGT 2 cut(s) 98, 192
TaqI TCGA 7 cut(s) 76, 87, 187, 235, 373, 379, 422
TasI AATT 3 cut(s) 78, 296, 323
TauI GCSGC 3 cut(s) 7, 24, 153
TfiI GAWTC 1 cut(s) 331
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 1 cut(s) 55
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 4 cut(s) 112, 195, 234, 323
Van91I CCANNNNNTGG 1 cut(s) 98
VpaK11BI GGWCC 1 cut(s) 104
XagI CCTNNNNNAGG 1 cut(s) 138
XapI RAATTY 1 cut(s) 296
XhoI CTCGAG 1 cut(s) 421
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.