RLG00000019388

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008185
Physical Location & Seq
Reverse (-)
51380753 .. 51388292
7540 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019388

Sequence Viewer

Length: 798 bp
ATGTCGTTCCCAAAACCTAGTCGCCTGTGCTCGGTGATCCAAACATGGCGACTGCTGCCGGTCTCTATTCTAAGTGGAGAAACCCACTCCACATCCCACAATCCAATCTCAGCCCTCCAATCCTGGTCGGCGACCAACCAACAAAATGAAGGTTATGCGACTTTCAGGGTTTCTGACCAGTGTCGATTTGGGGTCTATCCAGATCGCGACGGACCTCCAGGGGCTAGCCTCGGGCGCTTTCCTCTAAGAGGCAGTGGTGGCTTGCTCTTCGAAGCAAGTAGATCTCTTGCATCGAATCTCCAATCTACTTCATGTGGAGGGTCAAGAAGATTCTATTGGCCTCATCATGCATTGATCAAACCTTGGGACCTGAGGAAGATGGTGCAATCGGTGATTCATTGCCGAAGCAAGCCTGTACGGGAAGCTGAGTTGGATGAGTTTGGTCGGACTCGACCGCATCGGGGACGTCTCAAAGTCACAATTTTGGATCTGATGATGCTCTTCTTGTTCAGCAAAGCCAGCTGCTCCACCAAGATCCGACATCCTTATCTGCACCATCGGCGAGTTCACCGCCACTTGCAACAGCTGGCTCCAACTCAGCCTGAACAGCACCTCCACATCACTGGTGCTGCCGACGCCGCGTTTCAGGACAGGCAAGGAGCCTCCGATGGGCACGGCATGAAGGGTGGCGACCTGAGGTTGGAAGAATCGAACGTGCGGTGTGCCGCCTCAAGCATGGTGGCAGTGTGTCCATCTTCTTCTCCCACGGTAGCAGTTGTTCCGATGTCGTCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

266

Amino Acids

29.41

Weight (kDa)

9.93

Isoelectric Point (pI)

58.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 469
AccII CGCG 2 cut(s) 207, 641
AciI CCGC 5 cut(s) 455, 571, 639, 718, 726
AclWI GGATC 3 cut(s) 31, 495, 529
AcyI GRCGYC 2 cut(s) 466, 636
AfaI GTAC 1 cut(s) 417
AfiI CCNNNNNNNGG 5 cut(s) 31, 248, 461, 669, 700
AjnI CCWGG 2 cut(s) 122, 217
AjuI GAANNNNNNNTTGG 2 cut(s) 319, 351
AloI GAACNNNNNNTCC 2 cut(s) 597, 629
AluBI AGCT 3 cut(s) 425, 522, 586
AluI AGCT 3 cut(s) 425, 522, 586
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 67, 473
AlwI GGATC 3 cut(s) 31, 495, 529
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 1 cut(s) 338
ApeKI GCWGC 3 cut(s) 55, 522, 629
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 2 cut(s) 212, 367
AsuHPI GGTGA 3 cut(s) 46, 403, 560
AsuII TTCGAA 1 cut(s) 270
AsuNHI GCTAGC 1 cut(s) 224
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 2 cut(s) 212, 367
AxyI CCTNAGG 2 cut(s) 371, 695
BaeGI GKGCMC 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 3 cut(s) 42, 509, 616
BccI CCATC 4 cut(s) 373, 564, 662, 760
BceAI ACGGC 1 cut(s) 691
BciT130I CCWGG 2 cut(s) 124, 219
BclI TGATCA 1 cut(s) 354
BcoDI GTCTC 2 cut(s) 67, 473
BfaI CTAG 2 cut(s) 18, 225
BfoI RGCGCY 1 cut(s) 238
BglII AGATCT 1 cut(s) 281
BisI GCNGC 5 cut(s) 56, 523, 630, 639, 726
BlsI GCNGC 5 cut(s) 57, 524, 631, 640, 727
Bme1390I CCNGG 2 cut(s) 124, 219
Bme18I GGWCC 2 cut(s) 212, 367
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 2 cut(s) 212, 367
BmiI GGNNCC 3 cut(s) 368, 591, 661
BmrFI CCNGG 2 cut(s) 124, 219
BmsI GCATC 3 cut(s) 299, 466, 486
BmtI GCTAGC 1 cut(s) 228
BoxI GACNNNNGTC 1 cut(s) 180
BpmI CTGGAG 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 715
BsaHI GRCGYC 2 cut(s) 466, 636
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 4 cut(s) 218, 229, 362, 765
BsaXI ACNNNNNCTCC 2 cut(s) 597, 627
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 248, 461, 669, 700
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 2 cut(s) 178, 628
Bse21I CCTNAGG 2 cut(s) 371, 695
Bse3DI GCAATG 1 cut(s) 397
BseBI CCWGG 2 cut(s) 124, 219
BseDI CCNNGG 4 cut(s) 218, 229, 362, 765
BseGI GGATG 3 cut(s) 92, 439, 541
BseLI CCNNNNNNNGG 5 cut(s) 31, 248, 461, 669, 700
BseMI GCAATG 1 cut(s) 397
BseMII CTCAG 5 cut(s) 123, 362, 417, 611, 686
BseNI ACTGG 2 cut(s) 178, 628
BseSI GKGCMC 1 cut(s) 675
BseXI GCAGC 3 cut(s) 42, 509, 616
BsgI GTGCAG 1 cut(s) 536
Bsh1236I CGCG 2 cut(s) 207, 641
Bsh1285I CGRYCG 1 cut(s) 455
BshFI GGCC 1 cut(s) 340
BsiEI CGRYCG 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 2 cut(s) 380, 477
BslI CCNNNNNNNGG 5 cut(s) 31, 248, 461, 669, 700
BsmAI GTCTC 2 cut(s) 67, 473
BsmBI CGTCTC 1 cut(s) 473
BsmFI GGGAC 2 cut(s) 380, 477
BsnI GGCC 1 cut(s) 340
Bso31I GGTCTC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 230
Bsp119I TTCGAA 1 cut(s) 270
Bsp1286I GDGCHC 2 cut(s) 32, 675
Bsp143I GATC 6 cut(s) 36, 202, 281, 354, 487, 534
Bsp68I TCGCGA 1 cut(s) 207
BspACI CCGC 5 cut(s) 455, 571, 639, 718, 726
BspANI GGCC 1 cut(s) 340
BspCNI CTCAG 5 cut(s) 122, 363, 418, 610, 687
BspFNI CGCG 2 cut(s) 207, 641
BspLI GGNNCC 3 cut(s) 368, 591, 661
BspOI GCTAGC 1 cut(s) 228
BspPI GGATC 3 cut(s) 31, 495, 529
BspQI GCTCTTC 2 cut(s) 272, 506
BspT104I TTCGAA 1 cut(s) 270
BspTNI GGTCTC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 397
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 2 cut(s) 178, 628
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 4 cut(s) 218, 229, 362, 765
BssMI GATC 6 cut(s) 36, 202, 281, 354, 487, 534
BssNI GRCGYC 2 cut(s) 466, 636
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 2 cut(s) 124, 219
Bst4CI ACNGT 1 cut(s) 769
Bst6I CTCTTC 2 cut(s) 272, 506
BstACI GRCGYC 2 cut(s) 466, 636
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 5 cut(s) 226, 263, 410, 520, 588
BstDEI CTNAG 7 cut(s) 71, 109, 245, 371, 426, 597, 695
BstDSI CCRYGG 1 cut(s) 765
BstENI CCTNNNNNAGG 1 cut(s) 246
BstF5I GGATG 3 cut(s) 92, 439, 541
BstFNI CGCG 2 cut(s) 207, 641
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 6 cut(s) 39, 205, 284, 357, 490, 537
BstMAI GTCTC 2 cut(s) 67, 473
BstMBI GATC 6 cut(s) 36, 202, 281, 354, 487, 534
BstMCI CGRYCG 1 cut(s) 455
BstMWI GCNNNNNNNGC 8 cut(s) 55, 234, 258, 519, 559, 607, 635, 638
BstNI CCWGG 2 cut(s) 124, 219
BstPAI GACNNNNGTC 1 cut(s) 180
BstSCI CCNGG 2 cut(s) 122, 217
BstSLI GKGCMC 1 cut(s) 675
BstUI CGCG 2 cut(s) 207, 641
BstV1I GCAGC 3 cut(s) 42, 509, 616
BstX2I RGATCY 3 cut(s) 281, 487, 534
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 3 cut(s) 281, 487, 534
Bsu36I CCTNAGG 2 cut(s) 371, 695
BsuRI GGCC 1 cut(s) 340
BtgI CCRYGG 1 cut(s) 765
BtsCI GGATG 3 cut(s) 92, 439, 541
BtsI GCAGTG 2 cut(s) 259, 750
BtsIMutI CAGTG 4 cut(s) 185, 259, 621, 750
BtuMI TCGCGA 1 cut(s) 207
Cac8I GCNNGC 5 cut(s) 226, 263, 410, 520, 588
CfoI GCGC 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 2 cut(s) 212, 367
CseI GACGC 1 cut(s) 644
Csp6I GTAC 1 cut(s) 416
CspCI CAANNNNNGTGG 2 cut(s) 720, 755
CviAII CATG 5 cut(s) 45, 312, 347, 679, 736
CviQI GTAC 1 cut(s) 416
DdeI CTNAG 7 cut(s) 71, 109, 245, 371, 426, 597, 695
DpnI GATC 6 cut(s) 38, 204, 283, 356, 489, 536
DpnII GATC 6 cut(s) 36, 202, 281, 354, 487, 534
Eam1104I CTCTTC 2 cut(s) 272, 506
EarI CTCTTC 2 cut(s) 272, 506
Eco130I CCWWGG 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 2 cut(s) 212, 367
Eco81I CCTNAGG 2 cut(s) 371, 695
Eco88I CYCGRG 1 cut(s) 230
EcoNI CCTNNNNNAGG 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 367
EcoRII CCWGG 2 cut(s) 122, 217
EcoT14I CCWWGG 1 cut(s) 362
EcoT22I ATGCAT 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 362
Esp3I CGTCTC 1 cut(s) 473
FaeI CATG 5 cut(s) 48, 315, 350, 682, 739
FaiI YATR 6 cut(s) 46, 156, 313, 348, 680, 737
FaqI GGGAC 2 cut(s) 380, 477
FatI CATG 5 cut(s) 44, 311, 346, 678, 735
FbaI TGATCA 1 cut(s) 354
Fnu4HI GCNGC 5 cut(s) 56, 523, 630, 639, 726
FokI GGATG 3 cut(s) 79, 446, 528
Fsp4HI GCNGC 5 cut(s) 56, 523, 630, 639, 726
FspBI CTAG 2 cut(s) 18, 225
GlaI GCGC 1 cut(s) 236
GluI GCNGC 5 cut(s) 56, 523, 630, 639, 726
GsuI CTGGAG 1 cut(s) 201
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 1 cut(s) 340
HapII CCGG 1 cut(s) 59
HgaI GACGC 1 cut(s) 644
HhaI GCGC 1 cut(s) 237
Hin1I GRCGYC 2 cut(s) 466, 636
Hin1II CATG 5 cut(s) 48, 315, 350, 682, 739
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HinfI GANTC 5 cut(s) 295, 330, 394, 448, 707
HpaII CCGG 1 cut(s) 59
HphI GGTGA 3 cut(s) 46, 403, 560
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 7 cut(s) 175, 447, 492, 539, 667, 783, 797
Hpy188III TCNNGA 4 cut(s) 200, 206, 324, 647
Hpy8I GTNNAC 1 cut(s) 568
Hpy99I CGWCG 2 cut(s) 212, 638
HpyAV CCTTC 2 cut(s) 143, 676
HpyCH4III ACNGT 1 cut(s) 769
HpyCH4IV ACGT 2 cut(s) 466, 714
HpyCH4V TGCA 5 cut(s) 290, 350, 385, 553, 580
HpyF10VI GCNNNNNNNGC 8 cut(s) 55, 234, 258, 519, 559, 607, 635, 638
HpyF3I CTNAG 7 cut(s) 71, 109, 245, 371, 426, 597, 695
HpySE526I ACGT 2 cut(s) 466, 714
Hsp92I GRCGYC 2 cut(s) 466, 636
Hsp92II CATG 5 cut(s) 48, 315, 350, 682, 739
HspAI GCGC 1 cut(s) 235
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 6 cut(s) 36, 202, 281, 354, 487, 534
LguI GCTCTTC 2 cut(s) 272, 506
LmnI GCTCC 3 cut(s) 530, 595, 659
Lsp1109I GCAGC 3 cut(s) 42, 509, 616
LweI GCATC 3 cut(s) 299, 466, 486
MaeI CTAG 2 cut(s) 18, 225
MaeII ACGT 2 cut(s) 466, 714
MaeIII GTNAC 1 cut(s) 475
MalI GATC 6 cut(s) 38, 204, 283, 356, 489, 536
MboI GATC 6 cut(s) 36, 202, 281, 354, 487, 534
MboII GAAGA 7 cut(s) 259, 339, 388, 493, 716, 747, 750
MflI RGATCY 3 cut(s) 281, 487, 534
MhlI GDGCHC 2 cut(s) 32, 675
MluCI AATT 1 cut(s) 480
MlyI GAGTC 1 cut(s) 442
MmeI TCCRAC 5 cut(s) 411, 425, 562, 617, 681
Mph1103I ATGCAT 1 cut(s) 352
MspA1I CMGCKG 2 cut(s) 522, 586
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 2 cut(s) 124, 219
MvaI CCWGG 2 cut(s) 124, 219
MvnI CGCG 2 cut(s) 207, 641
MwoI GCNNNNNNNGC 8 cut(s) 55, 234, 258, 519, 559, 607, 635, 638
NdeII GATC 6 cut(s) 36, 202, 281, 354, 487, 534
NheI GCTAGC 1 cut(s) 224
NlaIII CATG 5 cut(s) 48, 315, 350, 682, 739
NlaIV GGNNCC 3 cut(s) 368, 591, 661
NmuCI GTSAC 1 cut(s) 475
NruI TCGCGA 1 cut(s) 207
NsiI ATGCAT 1 cut(s) 352
NspV TTCGAA 1 cut(s) 270
PciSI GCTCTTC 2 cut(s) 272, 506
PfeI GAWTC 4 cut(s) 295, 330, 394, 707
PkrI GCNGC 5 cut(s) 57, 524, 631, 640, 727
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
PpuMI RGGWCCY 1 cut(s) 367
PshAI GACNNNNGTC 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 367
Psp6I CCWGG 2 cut(s) 122, 217
PspGI CCWGG 2 cut(s) 122, 217
PspN4I GGNNCC 3 cut(s) 368, 591, 661
PspPI GGNCC 2 cut(s) 212, 367
PspPPI RGGWCCY 1 cut(s) 367
PsrI GAACNNNNNNTAC 2 cut(s) 762, 794
PsuI RGATCY 3 cut(s) 281, 487, 534
PvuII CAGCTG 2 cut(s) 522, 586
RruI TCGCGA 1 cut(s) 207
RsaI GTAC 1 cut(s) 417
RsaNI GTAC 1 cut(s) 416
SapI GCTCTTC 2 cut(s) 272, 506
SatI GCNGC 5 cut(s) 56, 523, 630, 639, 726
Sau3AI GATC 6 cut(s) 36, 202, 281, 354, 487, 534
Sau96I GGNCC 2 cut(s) 212, 367
SchI GAGTC 1 cut(s) 442
ScrFI CCNGG 2 cut(s) 124, 219
SduI GDGCHC 2 cut(s) 32, 675
SfaNI GCATC 3 cut(s) 299, 466, 486
SfuI TTCGAA 1 cut(s) 270
SinI GGWCC 2 cut(s) 212, 367
SmlI CTYRAG 1 cut(s) 730
SmoI CTYRAG 1 cut(s) 730
Sse9I AATT 1 cut(s) 480
SsiI CCGC 5 cut(s) 455, 571, 639, 718, 726
SspMI CTAG 2 cut(s) 18, 225
StyD4I CCNGG 2 cut(s) 122, 217
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 1 cut(s) 769
TaiI ACGT 2 cut(s) 469, 717
TaqI TCGA 5 cut(s) 184, 270, 293, 451, 710
TasI AATT 1 cut(s) 480
TauI GCSGC 2 cut(s) 641, 728
TfiI GAWTC 4 cut(s) 295, 330, 394, 707
TscAI CASTG 4 cut(s) 185, 259, 628, 750
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 3 cut(s) 55, 522, 629
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 4 cut(s) 162, 300, 386, 695
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 4 cut(s) 185, 259, 628, 750
VpaK11BI GGWCC 2 cut(s) 212, 367
XagI CCTNNNNNAGG 1 cut(s) 246
XcmI CCANNNNNNNNNTGG 1 cut(s) 185
XspI CTAG 2 cut(s) 18, 225
ZraI GACGTC 1 cut(s) 467
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.