Rroxscaffold_158G00443760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000158
OG0008185
Physical Location & Seq
Forward (+)
100253 .. 100678
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_158G00443760.1

Sequence Viewer

Length: 381 bp
ATGGTTGATGTAAATGAGTATGATCGGAGGTCGGATCTGTTGGGACCCAAGGATGAATGGGTACAAAATTGGGATTTTTGGATTCATCCAAGTCGGGCTCGCCTCAAATCGGACCAAATCTTGATGGGAGAAGCAAGCGGTGGTGAGAGGTCGTTCGATCATGGCGGTACGAGCACCTCTCGGGGGCCGGCCAAGACAAGCCGGGTCGGGCGAGTCTACGCCGTTTGGATTGGTACATGCGAGCCAAGCGGCTTGGAGAGGTTTCATGCTCCGTTCAATGTTGGTGGCAGCGAGCGGCTTTGCGTGTCTTCGGATGGTCATGACTTAATTGTCCAAGGCCGGGAGACTAGGGTCCAATATGCGGTGAAGCCGAGGCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

14.17

Weight (kDa)

7.85

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 329
AccBSI CCGCTC 1 cut(s) 295
AccI GTMKAC 1 cut(s) 216
AciI CCGC 5 cut(s) 138, 165, 249, 295, 362
AclWI GGATC 1 cut(s) 42
AcoI YGGCCR 1 cut(s) 189
AfaI GTAC 3 cut(s) 63, 169, 235
AfiI CCNNNNNNNGG 4 cut(s) 109, 183, 340, 361
AgsI TTSAA 1 cut(s) 277
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 1 cut(s) 338
AlwI GGATC 1 cut(s) 42
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 3 cut(s) 185, 189, 337
ApeKI GCWGC 1 cut(s) 288
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 4 cut(s) 44, 112, 185, 352
AsuC2I CCSGG 2 cut(s) 203, 341
AsuHPI GGTGA 2 cut(s) 155, 376
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 3 cut(s) 44, 112, 352
BanII GRGCYC 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 1 cut(s) 300
BccI CCATC 2 cut(s) 118, 308
BceAI ACGGC 1 cut(s) 206
BcnI CCSGG 2 cut(s) 203, 341
BcoDI GTCTC 1 cut(s) 338
BfaI CTAG 1 cut(s) 348
BfoI RGCGCY 1 cut(s) 379
BisI GCNGC 3 cut(s) 250, 289, 296
BlsI GCNGC 3 cut(s) 251, 290, 297
Bme1390I CCNGG 2 cut(s) 203, 341
Bme18I GGWCC 3 cut(s) 44, 112, 352
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 4 cut(s) 44, 112, 185, 352
BmiI GGNNCC 4 cut(s) 45, 46, 186, 353
BmrFI CCNGG 2 cut(s) 203, 341
BoxI GACNNNNGTC 1 cut(s) 350
BpiI GAAGAC 1 cut(s) 300
BplI GAGNNNNNCTC 2 cut(s) 163, 195
BpuMI CCSGG 2 cut(s) 203, 341
BsaJI CCNNGG 3 cut(s) 48, 334, 371
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 183, 340, 361
Bse118I RCCGGY 1 cut(s) 187
BseDI CCNNGG 3 cut(s) 48, 334, 371
BseGI GGATG 3 cut(s) 58, 85, 319
BseLI CCNNNNNNNGG 4 cut(s) 109, 183, 340, 361
BseXI GCAGC 1 cut(s) 300
BshFI GGCC 3 cut(s) 187, 191, 339
BsiHKAI GWGCWC 1 cut(s) 176
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 3 cut(s) 188, 202, 340
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 4 cut(s) 109, 183, 340, 361
BsmAI GTCTC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 57
BsnI GGCC 3 cut(s) 187, 191, 339
BsoBI CYCGRG 1 cut(s) 180
Bsp1286I GDGCHC 2 cut(s) 100, 176
Bsp143I GATC 3 cut(s) 22, 34, 157
BspACI CCGC 5 cut(s) 138, 165, 249, 295, 362
BspANI GGCC 3 cut(s) 187, 191, 339
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 4 cut(s) 45, 46, 186, 353
BspPI GGATC 1 cut(s) 42
BsrBI CCGCTC 1 cut(s) 295
BsrFI RCCGGY 1 cut(s) 187
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 3 cut(s) 48, 334, 371
BssMI GATC 3 cut(s) 22, 34, 157
BssT1I CCWWGG 2 cut(s) 48, 334
BstC8I GCNNGC 5 cut(s) 100, 136, 189, 242, 293
BstF5I GGATG 3 cut(s) 58, 85, 319
BstH2I RGCGCY 1 cut(s) 379
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 3 cut(s) 25, 37, 160
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 3 cut(s) 22, 34, 157
BstMWI GCNNNNNNNGC 2 cut(s) 171, 246
BstNSI RCATGY 1 cut(s) 240
BstPAI GACNNNNGTC 1 cut(s) 350
BstSCI CCNGG 2 cut(s) 201, 339
BstV1I GCAGC 1 cut(s) 300
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuRI GGCC 3 cut(s) 187, 191, 339
BtsCI GGATG 3 cut(s) 58, 85, 319
Cac8I GCNNGC 5 cut(s) 100, 136, 189, 242, 293
CciI TCATGA 1 cut(s) 319
CfoI GCGC 1 cut(s) 378
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 4 cut(s) 44, 112, 185, 352
Csp6I GTAC 3 cut(s) 62, 168, 234
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 4 cut(s) 161, 237, 266, 320
CviJI RGCY 9 cut(s) 98, 187, 191, 201, 244, 252, 298, 339, 370
CviKI_1 RGCY 9 cut(s) 98, 187, 191, 201, 244, 252, 298, 339, 370
CviQI GTAC 3 cut(s) 62, 168, 234
DpnI GATC 3 cut(s) 24, 36, 159
DpnII GATC 3 cut(s) 22, 34, 157
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
EaeI YGGCCR 1 cut(s) 189
Eco130I CCWWGG 2 cut(s) 48, 334
Eco24I GRGCYC 1 cut(s) 100
Eco47I GGWCC 3 cut(s) 44, 112, 352
Eco88I CYCGRG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 44
EcoT14I CCWWGG 2 cut(s) 48, 334
EcoT38I GRGCYC 1 cut(s) 100
ErhI CCWWGG 2 cut(s) 48, 334
FaeI CATG 4 cut(s) 164, 240, 269, 323
FaiI YATR 6 cut(s) 21, 162, 238, 267, 321, 360
FaqI GGGAC 1 cut(s) 57
FatI CATG 4 cut(s) 160, 236, 265, 319
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 250, 289, 296
FokI GGATG 3 cut(s) 65, 72, 326
FriOI GRGCYC 1 cut(s) 100
FseI GGCCGGCC 1 cut(s) 191
Fsp4HI GCNGC 3 cut(s) 250, 289, 296
FspBI CTAG 1 cut(s) 348
GlaI GCGC 1 cut(s) 377
GluI GCNGC 3 cut(s) 250, 289, 296
HaeII RGCGCY 1 cut(s) 379
HaeIII GGCC 3 cut(s) 187, 191, 339
HapII CCGG 3 cut(s) 188, 202, 340
HhaI GCGC 1 cut(s) 378
Hin1II CATG 4 cut(s) 164, 240, 269, 323
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HinfI GANTC 2 cut(s) 82, 213
HpaII CCGG 3 cut(s) 188, 202, 340
HphI GGTGA 2 cut(s) 155, 376
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 4 cut(s) 27, 34, 112, 313
Hpy188III TCNNGA 2 cut(s) 121, 320
Hpy8I GTNNAC 1 cut(s) 217
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 246
Hsp92II CATG 4 cut(s) 164, 240, 269, 323
HspAI GCGC 1 cut(s) 376
KflI GGGWCCC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 187
KroNI GCCGGC 1 cut(s) 189
Kzo9I GATC 3 cut(s) 22, 34, 157
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 3 cut(s) 201, 215, 353
Lsp1109I GCAGC 1 cut(s) 300
MaeI CTAG 1 cut(s) 348
MalI GATC 3 cut(s) 24, 36, 159
MbiI CCGCTC 1 cut(s) 295
MboI GATC 3 cut(s) 22, 34, 157
MboII GAAGA 1 cut(s) 300
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 2 cut(s) 100, 176
MluCI AATT 2 cut(s) 67, 327
MlyI GAGTC 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 12
MnlI CCTC 6 cut(s) 21, 113, 141, 187, 252, 366
MroNI GCCGGC 1 cut(s) 187
MseI TTAA 1 cut(s) 326
MspI CCGG 3 cut(s) 188, 202, 340
MspR9I CCNGG 2 cut(s) 203, 341
MwoI GCNNNNNNNGC 2 cut(s) 171, 246
NaeI GCCGGC 1 cut(s) 189
NciI CCSGG 2 cut(s) 203, 341
NdeII GATC 3 cut(s) 22, 34, 157
NgoMIV GCCGGC 1 cut(s) 187
NlaIII CATG 4 cut(s) 164, 240, 269, 323
NlaIV GGNNCC 4 cut(s) 45, 46, 186, 353
NspI RCATGY 1 cut(s) 240
PagI TCATGA 1 cut(s) 319
PdiI GCCGGC 1 cut(s) 189
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 3 cut(s) 251, 290, 297
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 44
PshAI GACNNNNGTC 1 cut(s) 350
Psp5II RGGWCCY 1 cut(s) 44
PspN4I GGNNCC 4 cut(s) 45, 46, 186, 353
PspPI GGNCC 4 cut(s) 44, 112, 185, 352
PspPPI RGGWCCY 1 cut(s) 44
PsuI RGATCY 1 cut(s) 34
RigI GGCCGGCC 1 cut(s) 191
RsaI GTAC 3 cut(s) 63, 169, 235
RsaNI GTAC 3 cut(s) 62, 168, 234
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 3 cut(s) 250, 289, 296
Sau3AI GATC 3 cut(s) 22, 34, 157
Sau96I GGNCC 4 cut(s) 44, 112, 185, 352
SchI GAGTC 1 cut(s) 222
ScrFI CCNGG 2 cut(s) 203, 341
SduI GDGCHC 2 cut(s) 100, 176
SetI ASST 4 cut(s) 32, 152, 179, 263
SinI GGWCC 3 cut(s) 44, 112, 352
Sse9I AATT 2 cut(s) 67, 327
SsiI CCGC 5 cut(s) 138, 165, 249, 295, 362
SspMI CTAG 1 cut(s) 348
StyD4I CCNGG 2 cut(s) 201, 339
StyI CCWWGG 2 cut(s) 48, 334
TaqI TCGA 1 cut(s) 156
TasI AATT 2 cut(s) 67, 327
TauI GCSGC 2 cut(s) 252, 298
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TseI GCWGC 1 cut(s) 288
TspDTI ATGAA 3 cut(s) 69, 74, 254
TspGWI ACGGA 1 cut(s) 261
VpaK11BI GGWCC 3 cut(s) 44, 112, 352
XceI RCATGY 1 cut(s) 240
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.