Rh2DG245300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008185
Physical Location & Seq
Reverse (-)
25094521 .. 25094880
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG245300.1

Sequence Viewer

Length: 360 bp
ATGGGTTGGATTCGTCCGAGGCGGGCTCGCCTCAAATCAGACCAAATCTTGATGGGAGAAGCGAGTACTGTATCAGGGGGTGATCATCTCTTCATCGACCTTATTTTTCGGAGGCGGCAGTGCAGATCATGGCACTGCCGCCTGAGAGCCTTTGGTGGCCGGCCAAGACAAGCTGGGTCAGGGGAAATGGCTACGCCGTTTGGGAGCGAGGGTTCTCGGATTTGCAGCAGCATGCCAAGCAGCTGGGAGAAGCTTCATGCTTCGTTCAATGATTGTGGCTGCCAGCGGCTTTGCGCGTCATCTGGATTGTCACGACCTTGTTGCCCAAAGCTAGGAGACTTGGGTCCAACATGCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

13.16

Weight (kDa)

9.79

Isoelectric Point (pI)

57.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 345
AccII CGCG 1 cut(s) 296
AciI CCGC 4 cut(s) 22, 115, 139, 286
AcoI YGGCCR 2 cut(s) 157, 161
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 332
AgsI TTSAA 1 cut(s) 268
AloI GAACNNNNNNTCC 2 cut(s) 196, 228
AluBI AGCT 4 cut(s) 173, 243, 253, 331
AluI AGCT 4 cut(s) 173, 243, 253, 331
Alw26I GTCTC 1 cut(s) 330
AoxI GGCC 2 cut(s) 157, 161
ApeKI GCWGC 4 cut(s) 225, 228, 240, 279
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 1 cut(s) 92
AvaII GGWCC 1 cut(s) 344
BanII GRGCYC 1 cut(s) 28
BbvI GCAGC 4 cut(s) 237, 240, 252, 266
BccI CCATC 1 cut(s) 46
BceAI ACGGC 1 cut(s) 181
BcgI CGANNNNNNTGC 2 cut(s) 303, 337
BclI TGATCA 1 cut(s) 82
BcoDI GTCTC 1 cut(s) 330
BfaI CTAG 1 cut(s) 332
BfuAI ACCTGC 1 cut(s) 345
BisI GCNGC 7 cut(s) 116, 139, 226, 229, 241, 280, 287
BlsI GCNGC 7 cut(s) 117, 140, 227, 230, 242, 281, 288
BmcAI AGTACT 1 cut(s) 67
Bme18I GGWCC 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 1 cut(s) 345
BoxI GACNNNNGTC 1 cut(s) 342
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BsaJI CCNNGG 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse118I RCCGGY 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 17
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMII CTCAG 1 cut(s) 134
BseXI GCAGC 4 cut(s) 237, 240, 252, 266
BseYI CCCAGC 2 cut(s) 173, 243
BsgI GTGCAG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 296
BshFI GGCC 2 cut(s) 159, 163
BsiSI CCGG 1 cut(s) 160
BslI CCNNNNNNNGG 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 330
BsnI GGCC 2 cut(s) 159, 163
Bsp1286I GDGCHC 1 cut(s) 28
Bsp143I GATC 2 cut(s) 82, 125
BspACI CCGC 4 cut(s) 22, 115, 139, 286
BspANI GGCC 2 cut(s) 159, 163
BspCNI CTCAG 1 cut(s) 135
BspFNI CGCG 1 cut(s) 296
BspLI GGNNCC 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 345
BsrFI RCCGGY 1 cut(s) 159
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 1 cut(s) 17
BssMI GATC 2 cut(s) 82, 125
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 1 cut(s) 95
BstC8I GCNNGC 5 cut(s) 24, 28, 161, 233, 284
BstDEI CTNAG 1 cut(s) 143
BstFNI CGCG 1 cut(s) 296
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 2 cut(s) 85, 128
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 2 cut(s) 82, 125
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstNSI RCATGY 2 cut(s) 235, 354
BstPAI GACNNNNGTC 1 cut(s) 342
BstUI CGCG 1 cut(s) 296
BstV1I GCAGC 4 cut(s) 237, 240, 252, 266
BstXI CCANNNNNNTGG 1 cut(s) 243
BsuRI GGCC 2 cut(s) 159, 163
BtsI GCAGTG 2 cut(s) 125, 133
BtsIMutI CAGTG 2 cut(s) 125, 133
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 5 cut(s) 24, 28, 161, 233, 284
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 344
CseI GACGC 1 cut(s) 285
Csp6I GTAC 1 cut(s) 66
CspCI CAANNNNNGTGG 2 cut(s) 256, 291
CviAII CATG 4 cut(s) 129, 232, 257, 351
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 1 cut(s) 143
DpnI GATC 2 cut(s) 84, 127
DpnII GATC 2 cut(s) 82, 125
EaeI YGGCCR 2 cut(s) 157, 161
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 344
EcoT38I GRGCYC 1 cut(s) 28
FaeI CATG 4 cut(s) 132, 235, 260, 354
FaiI YATR 4 cut(s) 130, 233, 258, 352
FatI CATG 4 cut(s) 128, 231, 256, 350
FauI CCCGC 1 cut(s) 15
FbaI TGATCA 1 cut(s) 82
Fnu4HI GCNGC 7 cut(s) 116, 139, 226, 229, 241, 280, 287
FriOI GRGCYC 1 cut(s) 28
FseI GGCCGGCC 1 cut(s) 163
Fsp4HI GCNGC 7 cut(s) 116, 139, 226, 229, 241, 280, 287
FspBI CTAG 1 cut(s) 332
GlaI GCGC 1 cut(s) 295
GluI GCNGC 7 cut(s) 116, 139, 226, 229, 241, 280, 287
GsaI CCCAGC 2 cut(s) 177, 247
HaeIII GGCC 2 cut(s) 159, 163
HapII CCGG 1 cut(s) 160
HgaI GACGC 1 cut(s) 285
HhaI GCGC 1 cut(s) 296
Hin1II CATG 4 cut(s) 132, 235, 260, 354
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 1 cut(s) 10
HpaII CCGG 1 cut(s) 160
HphI GGTGA 1 cut(s) 92
Hpy188I TCNGA 4 cut(s) 18, 40, 111, 219
Hpy188III TCNNGA 3 cut(s) 49, 303, 312
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4V TGCA 3 cut(s) 123, 225, 354
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpyF3I CTNAG 1 cut(s) 143
Hsp92II CATG 4 cut(s) 132, 235, 260, 354
HspAI GCGC 1 cut(s) 294
KroI GCCGGC 1 cut(s) 159
KroNI GCCGGC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 82
Kzo9I GATC 2 cut(s) 82, 125
LmnI GCTCC 1 cut(s) 204
LpnPI CCDG 9 cut(s) 60, 155, 159, 165, 173, 229, 288, 296, 340
Lsp1109I GCAGC 4 cut(s) 237, 240, 252, 266
MaeI CTAG 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 309
MalI GATC 2 cut(s) 84, 127
MboI GATC 2 cut(s) 82, 125
MboII GAAGA 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 28
MnlI CCTC 4 cut(s) 12, 41, 105, 202
MroNI GCCGGC 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 355
MspA1I CMGCKG 2 cut(s) 243, 286
MspI CCGG 1 cut(s) 160
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 1 cut(s) 237
NaeI GCCGGC 1 cut(s) 161
NdeII GATC 2 cut(s) 82, 125
NgoMIV GCCGGC 1 cut(s) 159
NlaIII CATG 4 cut(s) 132, 235, 260, 354
NlaIV GGNNCC 1 cut(s) 345
NmuCI GTSAC 1 cut(s) 309
NspI RCATGY 2 cut(s) 235, 354
PaeI GCATGC 1 cut(s) 235
PaqCI CACCTGC 1 cut(s) 345
PdiI GCCGGC 1 cut(s) 161
PfeI GAWTC 1 cut(s) 10
PkrI GCNGC 7 cut(s) 117, 140, 227, 230, 242, 281, 288
PshAI GACNNNNGTC 1 cut(s) 342
PspFI CCCAGC 2 cut(s) 173, 243
PspN4I GGNNCC 1 cut(s) 345
PspPI GGNCC 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 243
RigI GGCCGGCC 1 cut(s) 163
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
RseI CAYNNNNRTG 1 cut(s) 355
SatI GCNGC 7 cut(s) 116, 139, 226, 229, 241, 280, 287
Sau3AI GATC 2 cut(s) 82, 125
Sau96I GGNCC 1 cut(s) 344
ScaI AGTACT 1 cut(s) 67
SduI GDGCHC 1 cut(s) 28
SetI ASST 7 cut(s) 102, 175, 245, 255, 319, 333, 359
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 355
SphI GCATGC 1 cut(s) 235
SsiI CCGC 4 cut(s) 22, 115, 139, 286
SspMI CTAG 1 cut(s) 332
TaaI ACNGT 1 cut(s) 70
TaqI TCGA 1 cut(s) 96
TatI WGTACW 1 cut(s) 65
TauI GCSGC 3 cut(s) 118, 141, 289
TfiI GAWTC 1 cut(s) 10
TscAI CASTG 2 cut(s) 125, 140
TseFI GTSAC 1 cut(s) 309
TseI GCWGC 4 cut(s) 225, 228, 240, 279
Tsp45I GTSAC 1 cut(s) 309
TspDTI ATGAA 2 cut(s) 82, 245
TspRI CASTG 2 cut(s) 125, 140
VpaK11BI GGWCC 1 cut(s) 344
XceI RCATGY 2 cut(s) 235, 354
XspI CTAG 1 cut(s) 332
ZrmI AGTACT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.