Rmu_sc0000674.1_g000016

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000674.1
OG0008185
Physical Location & Seq
Reverse (-)
81288 .. 83697
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000674.1_g000016.1.cds

Sequence Viewer

Length: 735 bp
atgagcatgtggctcccgaaacctagccgcctctgtgtggtgctccaattgtggagatcggcgccggtctcctctctaactggaggttctctttcggtctttccacatcaggttgttggcatgcagccctccaacattgatgcctctcgactacaacccataaaatccaactactggatccgaccgaggagagggcgactcaaacctcctgatgactcgaaaactcgatggtctgggcttgacggtcttctgatttacccatggcagaggcttgcttttggtgaccaaaacagagcatcatggctcgctgcacttccaattgcagatgtgggggccgggacccgaatccgattgatggcggcgcgaatctttgctctccaatctgtggcagtggaggagatagtcgtcaagtggagaagccgagaatggcagagttgcaagagtttagaggaggaggaggagactccggagagaatgagagacgaactggagaatcgagaagtcgaagtcctctctgagagaagtggagaagccgataatggcagagctacaacctgcaggagtttagaggaggaggaggagactccggagagaatccgcccctgggccttggtgaatgcagagcatgctggcaaggtgaccggagatgagaagatggcagcgtctgctgcgtttggcaagggtgtagcaagttgggtgcccagatcatcttgggagcccagaaggattcgatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

27.63

Weight (kDa)

8.86

Isoelectric Point (pI)

62.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 563
AccB1I GGYRCC 2 cut(s) 61, 697
AccB7I CCANNNNNTGG 2 cut(s) 174, 385
AccII CGCG 1 cut(s) 364
AccIII TCCGGA 2 cut(s) 466, 586
AciI CCGC 3 cut(s) 28, 359, 598
AclWI GGATC 2 cut(s) 172, 185
AcyI GRCGYC 1 cut(s) 62
AfiI CCNNNNNNNGG 6 cut(s) 37, 174, 191, 355, 385, 604
AjnI CCWGG 1 cut(s) 602
AluBI AGCT 1 cut(s) 548
AluI AGCT 1 cut(s) 548
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 4 cut(s) 73, 455, 474, 575
AlwI GGATC 2 cut(s) 172, 185
AlwNI CAGNNNCTG 1 cut(s) 665
Aor13HI TCCGGA 2 cut(s) 466, 586
AoxI GGCC 2 cut(s) 333, 606
ApeKI GCWGC 4 cut(s) 124, 308, 659, 668
AspLEI GCGC 2 cut(s) 64, 364
AspS9I GGNCC 3 cut(s) 333, 339, 606
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 3 cut(s) 293, 625, 649
AvaII GGWCC 1 cut(s) 339
BaeGI GKGCMC 1 cut(s) 702
BamHI GGATCC 1 cut(s) 177
BanI GGYRCC 2 cut(s) 61, 697
BanII GRGCYC 1 cut(s) 720
BbsI GAAGAC 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 4 cut(s) 136, 295, 655, 671
BccI CCATC 3 cut(s) 222, 349, 649
BciT130I CCWGG 1 cut(s) 604
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 4 cut(s) 73, 455, 474, 575
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 556
BfoI RGCGCY 1 cut(s) 65
BfuAI ACCTGC 1 cut(s) 563
BisI GCNGC 6 cut(s) 28, 125, 309, 360, 660, 669
BlsI GCNGC 6 cut(s) 29, 126, 310, 361, 661, 670
Bme1390I CCNGG 2 cut(s) 337, 604
Bme18I GGWCC 1 cut(s) 339
BmgT120I GGNCC 3 cut(s) 333, 339, 606
BmiI GGNNCC 8 cut(s) 14, 63, 179, 334, 340, 341, 699, 717
BmrFI CCNGG 2 cut(s) 337, 604
BmsI GCATC 2 cut(s) 130, 305
BpiI GAAGAC 1 cut(s) 239
BplI GAGNNNNNCTC 2 cut(s) 183, 215
BpmI CTGGAG 2 cut(s) 102, 509
BpuMI CCSGG 1 cut(s) 337
BsaHI GRCGYC 1 cut(s) 62
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 5 cut(s) 185, 260, 602, 603, 609
BsaWI WCCGGW 3 cut(s) 466, 586, 641
Bsc4I CCNNNNNNNGG 6 cut(s) 37, 174, 191, 355, 385, 604
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 3 cut(s) 85, 179, 492
BseAI TCCGGA 2 cut(s) 466, 586
BseBI CCWGG 1 cut(s) 604
BseDI CCNNGG 5 cut(s) 185, 260, 602, 603, 609
BseLI CCNNNNNNNGG 6 cut(s) 37, 174, 191, 355, 385, 604
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 3 cut(s) 85, 179, 492
BseSI GKGCMC 1 cut(s) 702
BseXI GCAGC 4 cut(s) 136, 295, 655, 671
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 364
Bsh1285I CGRYCG 1 cut(s) 185
BshFI GGCC 2 cut(s) 335, 608
BshNI GGYRCC 2 cut(s) 61, 697
BsiEI CGRYCG 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 45
BsiSI CCGG 5 cut(s) 65, 336, 467, 587, 642
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 6 cut(s) 37, 174, 191, 355, 385, 604
BsmAI GTCTC 4 cut(s) 73, 455, 474, 575
BsmBI CGTCTC 1 cut(s) 474
BsmFI GGGAC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 2 cut(s) 335, 608
Bso31I GGTCTC 1 cut(s) 73
Bsp1286I GDGCHC 3 cut(s) 45, 702, 720
Bsp13I TCCGGA 2 cut(s) 466, 586
Bsp143I GATC 3 cut(s) 56, 177, 704
Bsp19I CCATGG 1 cut(s) 260
BspACI CCGC 3 cut(s) 28, 359, 598
BspANI GGCC 2 cut(s) 335, 608
BspCNI CTCAG 1 cut(s) 508
BspEI TCCGGA 2 cut(s) 466, 586
BspFNI CGCG 1 cut(s) 364
BspLI GGNNCC 8 cut(s) 14, 63, 179, 334, 340, 341, 699, 717
BspMAI CTGCAG 1 cut(s) 560
BspMI ACCTGC 1 cut(s) 563
BspPI GGATC 2 cut(s) 172, 185
BspT107I GGYRCC 2 cut(s) 61, 697
BspTNI GGTCTC 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 3 cut(s) 85, 179, 492
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 5 cut(s) 185, 260, 602, 603, 609
BssMI GATC 3 cut(s) 56, 177, 704
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 2 cut(s) 260, 609
Bst2UI CCWGG 1 cut(s) 604
Bst4CI ACNGT 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 62
BstAPI GCANNNNNTGC 2 cut(s) 626, 665
BstC8I GCNNGC 5 cut(s) 122, 273, 306, 627, 631
BstDEI CTNAG 1 cut(s) 516
BstDSI CCRYGG 1 cut(s) 260
BstEII GGTNACC 2 cut(s) 281, 637
BstFNI CGCG 1 cut(s) 364
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 2 cut(s) 64, 364
BstKTI GATC 3 cut(s) 59, 180, 707
BstMAI GTCTC 4 cut(s) 73, 455, 474, 575
BstMBI GATC 3 cut(s) 56, 177, 704
BstMCI CGRYCG 1 cut(s) 185
BstMWI GCNNNNNNNGC 3 cut(s) 626, 665, 668
BstNI CCWGG 1 cut(s) 604
BstNSI RCATGY 3 cut(s) 10, 124, 629
BstPI GGTNACC 2 cut(s) 281, 637
BstSCI CCNGG 2 cut(s) 335, 602
BstSFI CTRYAG 1 cut(s) 556
BstSLI GKGCMC 1 cut(s) 702
BstUI CGCG 1 cut(s) 364
BstV1I GCAGC 4 cut(s) 136, 295, 655, 671
BstV2I GAAGAC 1 cut(s) 239
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BsuRI GGCC 2 cut(s) 335, 608
BtgI CCRYGG 1 cut(s) 260
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 1 cut(s) 396
BveI ACCTGC 1 cut(s) 563
Cac8I GCNNGC 5 cut(s) 122, 273, 306, 627, 631
CaiI CAGNNNCTG 1 cut(s) 665
CfoI GCGC 2 cut(s) 64, 364
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 3 cut(s) 333, 339, 606
CseI GACGC 1 cut(s) 651
CviAII CATG 5 cut(s) 7, 121, 261, 300, 626
DdeI CTNAG 1 cut(s) 516
DinI GGCGCC 1 cut(s) 63
DpnI GATC 3 cut(s) 58, 179, 706
DpnII GATC 3 cut(s) 56, 177, 704
EciI GGCGGA 1 cut(s) 587
Eco130I CCWWGG 2 cut(s) 260, 609
Eco24I GRGCYC 1 cut(s) 720
Eco31I GGTCTC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 339
Eco91I GGTNACC 2 cut(s) 281, 637
EcoO109I RGGNCCY 1 cut(s) 339
EcoO65I GGTNACC 2 cut(s) 281, 637
EcoRII CCWGG 1 cut(s) 602
EcoT14I CCWWGG 2 cut(s) 260, 609
EcoT38I GRGCYC 1 cut(s) 720
EgeI GGCGCC 1 cut(s) 63
EheI GGCGCC 1 cut(s) 63
ErhI CCWWGG 2 cut(s) 260, 609
Esp3I CGTCTC 1 cut(s) 474
FaeI CATG 5 cut(s) 10, 124, 264, 303, 629
FaiI YATR 6 cut(s) 8, 122, 161, 262, 301, 627
FaqI GGGAC 1 cut(s) 352
FatI CATG 5 cut(s) 6, 120, 260, 299, 625
Fnu4HI GCNGC 6 cut(s) 28, 125, 309, 360, 660, 669
FriOI GRGCYC 1 cut(s) 720
Fsp4HI GCNGC 6 cut(s) 28, 125, 309, 360, 660, 669
FspBI CTAG 1 cut(s) 24
GlaI GCGC 2 cut(s) 63, 363
GluI GCNGC 6 cut(s) 28, 125, 309, 360, 660, 669
GsuI CTGGAG 2 cut(s) 102, 509
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 2 cut(s) 335, 608
HapII CCGG 5 cut(s) 65, 336, 467, 587, 642
HgaI GACGC 1 cut(s) 651
HhaI GCGC 2 cut(s) 64, 364
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 5 cut(s) 10, 124, 264, 303, 629
Hin6I GCGC 2 cut(s) 62, 362
HinP1I GCGC 2 cut(s) 62, 362
HinfI GANTC 9 cut(s) 198, 215, 345, 366, 463, 493, 583, 594, 727
HpaII CCGG 5 cut(s) 65, 336, 467, 587, 642
HphI GGTGA 3 cut(s) 293, 625, 649
Hpy188I TCNGA 4 cut(s) 182, 252, 350, 517
Hpy188III TCNNGA 6 cut(s) 16, 147, 209, 467, 497, 587
HpyAV CCTTC 1 cut(s) 717
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4V TGCA 6 cut(s) 124, 311, 323, 438, 558, 620
HpyF10VI GCNNNNNNNGC 3 cut(s) 626, 665, 668
HpyF3I CTNAG 1 cut(s) 516
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 5 cut(s) 10, 124, 264, 303, 629
HspAI GCGC 2 cut(s) 62, 362
KasI GGCGCC 1 cut(s) 61
KflI GGGWCCC 1 cut(s) 339
Kpn2I TCCGGA 2 cut(s) 466, 586
Kzo9I GATC 3 cut(s) 56, 177, 704
LmnI GCTCC 3 cut(s) 18, 48, 715
Lsp1109I GCAGC 4 cut(s) 136, 295, 655, 671
LweI GCATC 2 cut(s) 130, 305
MaeI CTAG 1 cut(s) 24
MaeIII GTNAC 2 cut(s) 281, 637
MalI GATC 3 cut(s) 58, 179, 706
MboI GATC 3 cut(s) 56, 177, 704
MboII GAAGA 2 cut(s) 239, 664
MfeI CAATTG 2 cut(s) 47, 318
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 3 cut(s) 45, 702, 720
MluCI AATT 2 cut(s) 47, 318
Mly113I GGCGCC 1 cut(s) 62
MlyI GAGTC 4 cut(s) 192, 209, 457, 577
MmeI TCCRAC 3 cut(s) 156, 192, 205
MroI TCCGGA 2 cut(s) 466, 586
MspI CCGG 5 cut(s) 65, 336, 467, 587, 642
MspR9I CCNGG 2 cut(s) 337, 604
MunI CAATTG 2 cut(s) 47, 318
Mva1269I GAATGC 1 cut(s) 622
MvaI CCWGG 1 cut(s) 604
MvnI CGCG 1 cut(s) 364
MwoI GCNNNNNNNGC 3 cut(s) 626, 665, 668
NarI GGCGCC 1 cut(s) 62
NciI CCSGG 1 cut(s) 337
NcoI CCATGG 1 cut(s) 260
NdeII GATC 3 cut(s) 56, 177, 704
NlaIII CATG 5 cut(s) 10, 124, 264, 303, 629
NlaIV GGNNCC 8 cut(s) 14, 63, 179, 334, 340, 341, 699, 717
NmeAIII GCCGAG 1 cut(s) 446
NmuCI GTSAC 2 cut(s) 281, 637
NspI RCATGY 3 cut(s) 10, 124, 629
PaeI GCATGC 2 cut(s) 124, 629
PasI CCCWGGG 1 cut(s) 603
PctI GAATGC 1 cut(s) 622
PfeI GAWTC 5 cut(s) 345, 366, 493, 594, 727
PflMI CCANNNNNTGG 2 cut(s) 174, 385
PkrI GCNGC 6 cut(s) 29, 126, 310, 361, 661, 670
PleI GAGTC 4 cut(s) 192, 209, 457, 577
PluTI GGCGCC 1 cut(s) 65
PpsI GAGTC 4 cut(s) 192, 209, 457, 577
PpuMI RGGWCCY 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 602
PspEI GGTNACC 2 cut(s) 281, 637
PspGI CCWGG 1 cut(s) 602
PspN4I GGNNCC 8 cut(s) 14, 63, 179, 334, 340, 341, 699, 717
PspPI GGNCC 3 cut(s) 333, 339, 606
PspPPI RGGWCCY 1 cut(s) 339
PstI CTGCAG 1 cut(s) 560
PstNI CAGNNNCTG 1 cut(s) 665
PsuI RGATCY 1 cut(s) 177
SatI GCNGC 6 cut(s) 28, 125, 309, 360, 660, 669
Sau3AI GATC 3 cut(s) 56, 177, 704
Sau96I GGNCC 3 cut(s) 333, 339, 606
SbfI CCTGCAGG 1 cut(s) 560
SchI GAGTC 4 cut(s) 192, 209, 457, 577
ScrFI CCNGG 2 cut(s) 337, 604
SdaI CCTGCAGG 1 cut(s) 560
SduI GDGCHC 3 cut(s) 45, 702, 720
SetI ASST 7 cut(s) 25, 88, 114, 208, 550, 557, 639
SfaNI GCATC 2 cut(s) 130, 305
SfcI CTRYAG 1 cut(s) 556
SfoI GGCGCC 1 cut(s) 63
SinI GGWCC 1 cut(s) 339
SphI GCATGC 2 cut(s) 124, 629
Sse8387I CCTGCAGG 1 cut(s) 560
Sse9I AATT 2 cut(s) 47, 318
SsiI CCGC 3 cut(s) 28, 359, 598
SspDI GGCGCC 1 cut(s) 61
SspMI CTAG 1 cut(s) 24
StyD4I CCNGG 2 cut(s) 335, 602
StyI CCWWGG 2 cut(s) 260, 609
TaaI ACNGT 1 cut(s) 245
TaqI TCGA 6 cut(s) 148, 218, 226, 496, 504, 730
TaqII GACCGA 2 cut(s) 85, 199
TasI AATT 2 cut(s) 47, 318
TauI GCSGC 2 cut(s) 30, 362
TfiI GAWTC 5 cut(s) 345, 366, 493, 594, 727
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 2 cut(s) 281, 637
TseI GCWGC 4 cut(s) 124, 308, 659, 668
Tsp45I GTSAC 2 cut(s) 281, 637
TspRI CASTG 1 cut(s) 396
Van91I CCANNNNNTGG 2 cut(s) 174, 385
VpaK11BI GGWCC 1 cut(s) 339
XceI RCATGY 3 cut(s) 10, 124, 629
XspI CTAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.