RLG00000018430

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007560
Physical Location & Seq
Reverse (-)
29691449 .. 29692540
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018430

Sequence Viewer

Length: 522 bp
ATGAGCTTCAAGAAGCTCAAAGCTTCAAACTTTGCCGTTGACAACACCGATCCGGAAACTGAAACCGAGACATCGGAGTACAATTACTTCCACAACATACCAAAATCCCAACCTGCCGCCCACATAAACCTCTCATATTCTCACTCGGAGAAGTACGCCCAGTCCGAAACTGAGAGATCAGAGTACGATTTCTTCGTCCCCAACCGTAACCCTAAAGCTCCGACCAAGGCTCAGTTGGAGAAGTATGCTCAGTCGAAGAGAGAAGAGTCGGTTCAGGCCGAGTCGGTTGCGGGCTCAGTGCCGCTAGCCCAGACGATGTCGAAGTATGCGCCGAGCATGAGGTCTGAGGGGTTGGAAGGGAGTACTTACTGGAGCAATGAAATCTCGAACATGAAAATGGTGGTGAGGCATAAAGAGTTGAAGGAGATTGTGGAGGAGATTAAGGAGAATTTCGATAAGGCCGCGGTGGCCGGAGACCAAGTTTCTGAGATGCTCGAGTCCAACCGGGCCCAGCTTGATTGA

Protein Analysis

174

Amino Acids

19.64

Weight (kDa)

5.24

Isoelectric Point (pI)

65.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 140 - 172 6e-06 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 121
AccII CGCG 1 cut(s) 464
AccIII TCCGGA 1 cut(s) 52
AciI CCGC 5 cut(s) 117, 290, 302, 462, 464
AclWI GGATC 1 cut(s) 44
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 1 cut(s) 448
AfaI GTAC 4 cut(s) 80, 155, 185, 364
AgsI TTSAA 3 cut(s) 10, 27, 421
AluBI AGCT 5 cut(s) 6, 16, 23, 218, 514
AluI AGCT 5 cut(s) 6, 16, 23, 218, 514
Alw26I GTCTC 2 cut(s) 62, 468
AlwI GGATC 1 cut(s) 44
Ama87I CYCGRG 1 cut(s) 494
Aor13HI TCCGGA 1 cut(s) 52
AoxI GGCC 4 cut(s) 276, 459, 468, 507
ApaI GGGCCC 1 cut(s) 511
ApoI RAATTY 1 cut(s) 448
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 2 cut(s) 507, 508
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 1 cut(s) 415
AsuNHI GCTAGC 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 511
BanII GRGCYC 2 cut(s) 296, 511
BarI GAAGNNNNNNTAC 4 cut(s) 71, 103, 176, 208
BceAI ACGGC 1 cut(s) 20
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BcnI CCSGG 1 cut(s) 506
BcoDI GTCTC 2 cut(s) 62, 468
BfaI CTAG 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 121
BglI GCCNNNNNGGC 1 cut(s) 467
BisI GCNGC 3 cut(s) 117, 302, 462
BlsI GCNGC 3 cut(s) 118, 303, 463
BmcAI AGTACT 1 cut(s) 364
Bme1390I CCNGG 1 cut(s) 506
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 2 cut(s) 507, 508
BmiI GGNNCC 1 cut(s) 509
BmrFI CCNGG 1 cut(s) 506
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 1 cut(s) 480
BmtI GCTAGC 1 cut(s) 308
BmuI ACTGGG 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 391
BpuMI CCSGG 1 cut(s) 506
BsaI GGTCTC 1 cut(s) 468
BsaJI CCNNGG 2 cut(s) 225, 462
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 465, 495
Bse1I ACTGG 2 cut(s) 160, 374
Bse3DI GCAATG 1 cut(s) 382
BseAI TCCGGA 1 cut(s) 52
BseDI CCNNGG 2 cut(s) 225, 462
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 6 cut(s) 162, 245, 263, 309, 336, 477
BseNI ACTGG 2 cut(s) 160, 374
BseRI GAGGAG 1 cut(s) 449
BseSI GKGCMC 1 cut(s) 511
BseYI CCCAGC 1 cut(s) 510
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 4 cut(s) 278, 461, 470, 509
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 3 cut(s) 53, 471, 505
BslFI GGGAC 1 cut(s) 182
BsmAI GTCTC 2 cut(s) 62, 468
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 4 cut(s) 278, 461, 470, 509
Bso31I GGTCTC 1 cut(s) 468
BsoBI CYCGRG 1 cut(s) 494
Bsp120I GGGCCC 1 cut(s) 507
Bsp1286I GDGCHC 2 cut(s) 296, 511
Bsp13I TCCGGA 1 cut(s) 52
Bsp143I GATC 2 cut(s) 49, 176
BspACI CCGC 5 cut(s) 117, 290, 302, 462, 464
BspANI GGCC 4 cut(s) 278, 461, 470, 509
BspCNI CTCAG 6 cut(s) 163, 244, 262, 308, 337, 478
BspEI TCCGGA 1 cut(s) 52
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 1 cut(s) 509
BspMI ACCTGC 1 cut(s) 121
BspOI GCTAGC 1 cut(s) 308
BspPI GGATC 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 382
BsrI ACTGG 2 cut(s) 160, 374
BssECI CCNNGG 2 cut(s) 225, 462
BssMI GATC 2 cut(s) 49, 176
BssT1I CCWWGG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 206
Bst6I CTCTTC 2 cut(s) 251, 258
BstC8I GCNNGC 2 cut(s) 292, 306
BstDEI CTNAG 6 cut(s) 171, 231, 249, 295, 345, 486
BstDSI CCRYGG 1 cut(s) 462
BstFNI CGCG 1 cut(s) 464
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 2 cut(s) 52, 179
BstMAI GTCTC 2 cut(s) 62, 468
BstMBI GATC 2 cut(s) 49, 176
BstMWI GCNNNNNNNGC 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 504
BstSLI GKGCMC 1 cut(s) 511
BstUI CGCG 1 cut(s) 464
BsuRI GGCC 4 cut(s) 278, 461, 470, 509
BtgI CCRYGG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 303
BveI ACCTGC 1 cut(s) 121
Cac8I GCNNGC 2 cut(s) 292, 306
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 507, 508
Cfr42I CCGCGG 1 cut(s) 465
Csp6I GTAC 4 cut(s) 79, 154, 184, 363
CviAII CATG 2 cut(s) 337, 391
CviQI GTAC 4 cut(s) 79, 154, 184, 363
DdeI CTNAG 6 cut(s) 171, 231, 249, 295, 345, 486
DpnI GATC 2 cut(s) 51, 178
DpnII GATC 2 cut(s) 49, 176
EaeI YGGCCR 1 cut(s) 468
Eam1104I CTCTTC 2 cut(s) 251, 258
EarI CTCTTC 2 cut(s) 251, 258
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 2 cut(s) 296, 511
Eco31I GGTCTC 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 494
EcoT14I CCWWGG 1 cut(s) 225
EcoT38I GRGCYC 2 cut(s) 296, 511
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 2 cut(s) 340, 394
FaiI YATR 8 cut(s) 98, 125, 136, 246, 327, 338, 392, 411
FaqI GGGAC 1 cut(s) 182
FatI CATG 2 cut(s) 336, 390
FauI CCCGC 1 cut(s) 283
Fnu4HI GCNGC 3 cut(s) 117, 302, 462
FriOI GRGCYC 2 cut(s) 296, 511
Fsp4HI GCNGC 3 cut(s) 117, 302, 462
FspBI CTAG 1 cut(s) 305
GlaI GCGC 1 cut(s) 330
GluI GCNGC 3 cut(s) 117, 302, 462
GsaI CCCAGC 1 cut(s) 514
GsuI CTGGAG 1 cut(s) 391
HaeIII GGCC 4 cut(s) 278, 461, 470, 509
HapII CCGG 3 cut(s) 53, 471, 505
HhaI GCGC 1 cut(s) 331
Hin1II CATG 2 cut(s) 340, 394
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 3 cut(s) 266, 281, 497
HpaII CCGG 3 cut(s) 53, 471, 505
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 7 cut(s) 76, 148, 166, 181, 222, 346, 487
Hpy188III TCNNGA 3 cut(s) 10, 53, 385
Hpy8I GTNNAC 1 cut(s) 40
HpyAV CCTTC 2 cut(s) 350, 415
HpyCH4III ACNGT 1 cut(s) 206
HpyF10VI GCNNNNNNNGC 1 cut(s) 467
HpyF3I CTNAG 6 cut(s) 171, 231, 249, 295, 345, 486
Hsp92II CATG 2 cut(s) 340, 394
HspAI GCGC 1 cut(s) 329
Kpn2I TCCGGA 1 cut(s) 52
KspI CCGCGG 1 cut(s) 465
Kzo9I GATC 2 cut(s) 49, 176
LmnI GCTCC 2 cut(s) 223, 372
LpnPI CCDG 8 cut(s) 66, 126, 173, 260, 323, 355, 484, 518
LweI GCATC 1 cut(s) 480
MaeI CTAG 1 cut(s) 305
MaeIII GTNAC 1 cut(s) 206
MalI GATC 2 cut(s) 51, 178
MboI GATC 2 cut(s) 49, 176
MboII GAAGA 3 cut(s) 184, 268, 275
MhlI GDGCHC 2 cut(s) 296, 511
MluCI AATT 2 cut(s) 82, 448
MlyI GAGTC 3 cut(s) 275, 290, 506
MmeI TCCRAC 3 cut(s) 216, 245, 333
MnlI CCTC 5 cut(s) 140, 333, 340, 399, 427
MroI TCCGGA 1 cut(s) 52
MseI TTAA 1 cut(s) 441
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 3 cut(s) 53, 471, 505
MspR9I CCNGG 1 cut(s) 506
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 1 cut(s) 467
NciI CCSGG 1 cut(s) 506
NdeII GATC 2 cut(s) 49, 176
NheI GCTAGC 1 cut(s) 304
NlaIII CATG 2 cut(s) 340, 394
NlaIV GGNNCC 1 cut(s) 509
NmeAIII GCCGAG 2 cut(s) 304, 357
PaeR7I CTCGAG 1 cut(s) 494
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 192
PflFI GACNNNGTC 1 cut(s) 316
PkrI GCNGC 3 cut(s) 118, 303, 463
PleI GAGTC 3 cut(s) 274, 289, 505
PpsI GAGTC 3 cut(s) 274, 289, 505
PspFI CCCAGC 1 cut(s) 510
PspN4I GGNNCC 1 cut(s) 509
PspOMI GGGCCC 1 cut(s) 507
PspPI GGNCC 2 cut(s) 507, 508
PspXI VCTCGAGB 1 cut(s) 494
PsyI GACNNNGTC 1 cut(s) 316
RsaI GTAC 4 cut(s) 80, 155, 185, 364
RsaNI GTAC 4 cut(s) 79, 154, 184, 363
RseI CAYNNNNRTG 1 cut(s) 395
SacII CCGCGG 1 cut(s) 465
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 3 cut(s) 117, 302, 462
Sau3AI GATC 2 cut(s) 49, 176
Sau96I GGNCC 2 cut(s) 507, 508
ScaI AGTACT 1 cut(s) 364
SchI GAGTC 3 cut(s) 275, 290, 506
ScrFI CCNGG 1 cut(s) 506
SduI GDGCHC 2 cut(s) 296, 511
SetI ASST 8 cut(s) 8, 18, 25, 115, 132, 220, 344, 516
SfaNI GCATC 1 cut(s) 480
SfiI GGCCNNNNNGGCC 1 cut(s) 467
Sfr274I CTCGAG 1 cut(s) 494
Sfr303I CCGCGG 1 cut(s) 465
SgrBI CCGCGG 1 cut(s) 465
SlaI CTCGAG 1 cut(s) 494
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 2 cut(s) 82, 448
SsiI CCGC 5 cut(s) 117, 290, 302, 462, 464
SspMI CTAG 1 cut(s) 305
StyD4I CCNGG 1 cut(s) 504
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 206
TaqI TCGA 5 cut(s) 254, 320, 386, 453, 495
TasI AATT 2 cut(s) 82, 448
TatI WGTACW 2 cut(s) 78, 362
TauI GCSGC 3 cut(s) 119, 304, 464
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 1 cut(s) 303
TspDTI ATGAA 2 cut(s) 393, 407
TspRI CASTG 1 cut(s) 303
Tth111I GACNNNGTC 1 cut(s) 316
XapI RAATTY 1 cut(s) 448
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XhoI CTCGAG 1 cut(s) 494
XspI CTAG 1 cut(s) 305
ZrmI AGTACT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.