RLG00000019889

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007560
Physical Location & Seq
Reverse (-)
58164820 .. 58165281
462 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019889

Sequence Viewer

Length: 387 bp
ATGGAGCTCGGTGGCTTTTCTCCAAGGCCATGGAAGTCGCGGGCACATGTCCCATGGTTTGGGATGGTTTCGGTTACCTCCAGATTTATTCCAAACTATAGCGGAGGGGTGGAGGGGGAGGTCGACGACTTAGGGTGCTGCGGATCAGCATCCGATGGGATTTGGGTTCGTGTTCGGTACGCCGGGTCTATCTCAGACTCTGGGAGCGTCGTTGAGGTAGTGAAACACTGGGGTGTGCTTAGACTGGTGGTGGAGGTAGTGGCAGGGGTGGTGGTCGCGTTTTGCAGCTGTGCAACGTCTACCTTAGTCGAGGAGGTGGTGTTTGGGCCTGAGATGGGCTTTTCCCATTTTGGACCCTGGGTTGGCTTTTCTTTTTGGGCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

129

Amino Acids

13.78

Weight (kDa)

5.21

Isoelectric Point (pI)

21.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 59
AccI GTMKAC 2 cut(s) 123, 299
AccII CGCG 2 cut(s) 40, 278
AciI CCGC 3 cut(s) 40, 102, 141
AclWI GGATC 1 cut(s) 151
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 3 cut(s) 59, 335, 362
AflIII ACRYGT 1 cut(s) 46
AjnI CCWGG 1 cut(s) 356
AleI CACNNNNGTG 1 cut(s) 231
AluBI AGCT 2 cut(s) 7, 288
AluI AGCT 2 cut(s) 7, 288
Alw21I GWGCWC 1 cut(s) 9
AlwI GGATC 1 cut(s) 151
AlwNI CAGNNNCTG 1 cut(s) 200
AoxI GGCC 2 cut(s) 26, 326
ApeKI GCWGC 2 cut(s) 138, 285
AspS9I GGNCC 2 cut(s) 326, 353
AsuC2I CCSGG 1 cut(s) 184
AvaII GGWCC 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 46
BanII GRGCYC 2 cut(s) 9, 382
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 125, 297
BccI CCATC 3 cut(s) 58, 149, 328
BciT130I CCWGG 1 cut(s) 358
BcnI CCSGG 1 cut(s) 184
BfmI CTRYAG 1 cut(s) 97
BisI GCNGC 2 cut(s) 139, 286
BlsI GCNGC 2 cut(s) 140, 287
Bme1390I CCNGG 2 cut(s) 184, 358
Bme18I GGWCC 1 cut(s) 353
BmgT120I GGNCC 2 cut(s) 326, 353
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 2 cut(s) 184, 358
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 158
BmuI ACTGGG 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 64
BpuMI CCSGG 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 148
BsaJI CCNNGG 5 cut(s) 23, 29, 53, 356, 357
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 335, 362
Bse1I ACTGG 2 cut(s) 233, 249
Bse8I GATNNNNATC 1 cut(s) 148
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 5 cut(s) 23, 29, 53, 356, 357
BseGI GGATG 2 cut(s) 69, 149
BseJI GATNNNNATC 1 cut(s) 148
BseLI CCNNNNNNNGG 3 cut(s) 59, 335, 362
BseMII CTCAG 2 cut(s) 207, 321
BseNI ACTGG 2 cut(s) 233, 249
BseRI GAGGAG 1 cut(s) 326
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 2 cut(s) 125, 297
Bsh1236I CGCG 2 cut(s) 40, 278
BshFI GGCC 2 cut(s) 28, 328
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 183
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 3 cut(s) 59, 335, 362
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 2 cut(s) 28, 328
Bsp1286I GDGCHC 3 cut(s) 9, 46, 382
Bsp143I GATC 1 cut(s) 143
Bsp19I CCATGG 2 cut(s) 29, 53
BspACI CCGC 3 cut(s) 40, 102, 141
BspANI GGCC 2 cut(s) 28, 328
BspCNI CTCAG 2 cut(s) 206, 322
BspFNI CGCG 2 cut(s) 40, 278
BspLI GGNNCC 1 cut(s) 355
BspPI GGATC 1 cut(s) 151
BsrI ACTGG 2 cut(s) 233, 249
BssECI CCNNGG 5 cut(s) 23, 29, 53, 356, 357
BssMI GATC 1 cut(s) 143
BssT1I CCWWGG 3 cut(s) 23, 29, 53
Bst2UI CCWGG 1 cut(s) 358
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 5 cut(s) 130, 193, 239, 304, 330
BstDSI CCRYGG 2 cut(s) 29, 53
BstEII GGTNACC 1 cut(s) 73
BstF5I GGATG 2 cut(s) 69, 149
BstFNI CGCG 2 cut(s) 40, 278
BstKTI GATC 1 cut(s) 146
BstMBI GATC 1 cut(s) 143
BstNI CCWGG 1 cut(s) 358
BstNSI RCATGY 1 cut(s) 50
BstPI GGTNACC 1 cut(s) 73
BstSCI CCNGG 2 cut(s) 182, 356
BstSFI CTRYAG 1 cut(s) 97
BstSLI GKGCMC 1 cut(s) 46
BstUI CGCG 2 cut(s) 40, 278
BstV1I GCAGC 2 cut(s) 125, 297
BstXI CCANNNNNNTGG 1 cut(s) 30
BsuRI GGCC 2 cut(s) 28, 328
BtgI CCRYGG 2 cut(s) 29, 53
BtsCI GGATG 2 cut(s) 69, 149
BtsIMutI CAGTG 1 cut(s) 226
Cac8I GCNNGC 1 cut(s) 42
CaiI CAGNNNCTG 1 cut(s) 200
Cfr13I GGNCC 2 cut(s) 326, 353
CseI GACGC 1 cut(s) 196
Csp6I GTAC 1 cut(s) 178
CviAII CATG 3 cut(s) 30, 47, 54
CviJI RGCY 8 cut(s) 7, 15, 28, 288, 328, 339, 366, 380
CviKI_1 RGCY 8 cut(s) 7, 15, 28, 288, 328, 339, 366, 380
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 5 cut(s) 130, 193, 239, 304, 330
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 3 cut(s) 23, 29, 53
Eco24I GRGCYC 2 cut(s) 9, 382
Eco47I GGWCC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 7
Eco91I GGTNACC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 7
EcoO65I GGTNACC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 3 cut(s) 23, 29, 53
EcoT38I GRGCYC 2 cut(s) 9, 382
ErhI CCWWGG 3 cut(s) 23, 29, 53
FaeI CATG 3 cut(s) 33, 50, 57
FaiI YATR 4 cut(s) 31, 48, 55, 99
FaqI GGGAC 1 cut(s) 35
FatI CATG 3 cut(s) 29, 46, 53
FauI CCCGC 1 cut(s) 33
FblI GTMKAC 2 cut(s) 123, 299
Fnu4HI GCNGC 2 cut(s) 139, 286
FokI GGATG 2 cut(s) 76, 136
FriOI GRGCYC 2 cut(s) 9, 382
Fsp4HI GCNGC 2 cut(s) 139, 286
GluI GCNGC 2 cut(s) 139, 286
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 2 cut(s) 28, 328
HapII CCGG 1 cut(s) 183
HgaI GACGC 1 cut(s) 196
Hin1II CATG 3 cut(s) 33, 50, 57
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 1 cut(s) 197
HpaII CCGG 1 cut(s) 183
Hpy166II GTNNAC 2 cut(s) 124, 300
Hpy188I TCNGA 2 cut(s) 154, 196
Hpy188III TCNNGA 1 cut(s) 81
Hpy8I GTNNAC 2 cut(s) 124, 300
Hpy99I CGWCG 2 cut(s) 128, 212
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 2 cut(s) 285, 293
HpyF3I CTNAG 5 cut(s) 130, 193, 239, 304, 330
HpySE526I ACGT 1 cut(s) 296
Hsp92II CATG 3 cut(s) 33, 50, 57
Kzo9I GATC 1 cut(s) 143
LmnI GCTCC 2 cut(s) 4, 204
LpnPI CCDG 9 cut(s) 94, 186, 196, 214, 230, 249, 342, 343, 370
Lsp1109I GCAGC 2 cut(s) 125, 297
LweI GCATC 1 cut(s) 158
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 73
MalI GATC 1 cut(s) 145
MboI GATC 1 cut(s) 143
MhlI GDGCHC 3 cut(s) 9, 46, 382
MlyI GAGTC 1 cut(s) 191
MnlI CCTC 8 cut(s) 88, 98, 106, 112, 208, 247, 304, 307
MslI CAYNNNNRTG 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 184, 358
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 2 cut(s) 40, 278
NciI CCSGG 1 cut(s) 184
NcoI CCATGG 2 cut(s) 29, 53
NdeII GATC 1 cut(s) 143
NlaIII CATG 3 cut(s) 33, 50, 57
NlaIV GGNNCC 1 cut(s) 355
NspI RCATGY 1 cut(s) 50
OliI CACNNNNGTG 1 cut(s) 231
PasI CCCWGGG 1 cut(s) 357
PciI ACATGT 1 cut(s) 46
PflMI CCANNNNNTGG 1 cut(s) 59
PkrI GCNGC 2 cut(s) 140, 287
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PscI ACATGT 1 cut(s) 46
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 356
PspEI GGTNACC 1 cut(s) 73
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 1 cut(s) 355
PspPI GGNCC 2 cut(s) 326, 353
PstNI CAGNNNCTG 1 cut(s) 200
PvuII CAGCTG 1 cut(s) 288
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
RseI CAYNNNNRTG 1 cut(s) 231
SacI GAGCTC 1 cut(s) 9
SalI GTCGAC 1 cut(s) 122
SatI GCNGC 2 cut(s) 139, 286
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 2 cut(s) 326, 353
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 2 cut(s) 184, 358
SduI GDGCHC 3 cut(s) 9, 46, 382
SetI ASST 9 cut(s) 9, 80, 123, 219, 258, 290, 299, 305, 318
SfaNI GCATC 1 cut(s) 158
SfcI CTRYAG 1 cut(s) 97
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 231
SsiI CCGC 3 cut(s) 40, 102, 141
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 182, 356
StyI CCWWGG 3 cut(s) 23, 29, 53
TaiI ACGT 1 cut(s) 299
TaqI TCGA 2 cut(s) 123, 309
TscAI CASTG 1 cut(s) 233
TseI GCWGC 2 cut(s) 138, 285
TspRI CASTG 1 cut(s) 233
Van91I CCANNNNNTGG 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 353
XceI RCATGY 1 cut(s) 50
XmiI GTMKAC 2 cut(s) 123, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.