RLG00000034055

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007560
Physical Location & Seq
Forward (+)
35906568 .. 35907880
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034055

Sequence Viewer

Length: 321 bp
ATGATCCCAGCAGCCGAGTTTGTTTTTCCGAGATCCGAGATGGGGACCCGATCCAATTTCGACCCTTCGGCTCAGGCCGAGTCGGTTGCGGGCTCAGTGCCGCCAGCCCAGATGATGTCGAAGTATGCGTCGAGCATGAGGTCTGAGGGGTTAGAAGGGAGTACTTACCGGAGCAATGAAATCTCGAACATGAAAATGGTGGTGAGGCATAAAGAGTTGAAGGAGATTGTGGAGGAGATTAAGGAGAATTTTTATAAGGCCGCGATGGCCGGAGACCAAGTTTCCGAGATGCTCGAGTCCAACCGAGCCCAGCTTGATTGA

Protein Analysis

107

Amino Acids

11.8

Weight (kDa)

5.05

Isoelectric Point (pI)

65.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 73 - 105 2.7e-06 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 255
AccII CGCG 1 cut(s) 263
AciI CCGC 3 cut(s) 89, 101, 261
AclWI GGATC 2 cut(s) 27, 45
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 1 cut(s) 247
AfaI GTAC 1 cut(s) 163
AfiI CCNNNNNNNGG 1 cut(s) 42
AgsI TTSAA 1 cut(s) 220
AluBI AGCT 1 cut(s) 313
AluI AGCT 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 2 cut(s) 27, 45
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 3 cut(s) 75, 258, 267
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 1 cut(s) 247
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AspS9I GGNCC 1 cut(s) 45
AsuHPI GGTGA 1 cut(s) 214
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 1 cut(s) 45
BanII GRGCYC 2 cut(s) 95, 310
BbvI GCAGC 1 cut(s) 23
BccI CCATC 2 cut(s) 34, 259
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BcoDI GTCTC 1 cut(s) 267
BglI GCCNNNNNGGC 1 cut(s) 266
BisI GCNGC 3 cut(s) 12, 101, 261
BlsI GCNGC 3 cut(s) 13, 102, 262
BmcAI AGTACT 1 cut(s) 163
Bme18I GGWCC 1 cut(s) 45
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 2 cut(s) 46, 47
BmsI GCATC 1 cut(s) 279
Bpu10I CCTNAGC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 267
BsaWI WCCGGW 1 cut(s) 168
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 181
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMI GCAATG 1 cut(s) 181
BseMII CTCAG 3 cut(s) 86, 108, 135
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 1 cut(s) 23
BseYI CCCAGC 2 cut(s) 7, 309
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 3 cut(s) 77, 260, 269
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 2 cut(s) 169, 270
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 3 cut(s) 77, 260, 269
Bso31I GGTCTC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 293
Bsp1286I GDGCHC 2 cut(s) 95, 310
Bsp143I GATC 3 cut(s) 3, 32, 50
BspACI CCGC 3 cut(s) 89, 101, 261
BspANI GGCC 3 cut(s) 77, 260, 269
BspCNI CTCAG 3 cut(s) 85, 107, 136
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 2 cut(s) 46, 47
BspPI GGATC 2 cut(s) 27, 45
BspTNI GGTCTC 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 181
BssMI GATC 3 cut(s) 3, 32, 50
BstC8I GCNNGC 2 cut(s) 91, 105
BstDEI CTNAG 3 cut(s) 72, 94, 144
BstFNI CGCG 1 cut(s) 263
BstKTI GATC 3 cut(s) 6, 35, 53
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 3 cut(s) 3, 32, 50
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BsuRI GGCC 3 cut(s) 77, 260, 269
BtgZI GCGATG 1 cut(s) 278
BtsIMutI CAGTG 1 cut(s) 102
Cac8I GCNNGC 2 cut(s) 91, 105
Cfr13I GGNCC 1 cut(s) 45
CseI GACGC 1 cut(s) 117
Csp6I GTAC 1 cut(s) 162
CviAII CATG 2 cut(s) 136, 190
CviJI RGCY 9 cut(s) 14, 71, 77, 93, 107, 260, 269, 308, 313
CviKI_1 RGCY 9 cut(s) 14, 71, 77, 93, 107, 260, 269, 308, 313
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 3 cut(s) 72, 94, 144
DpnI GATC 3 cut(s) 5, 34, 52
DpnII GATC 3 cut(s) 3, 32, 50
EaeI YGGCCR 1 cut(s) 267
Eco24I GRGCYC 2 cut(s) 95, 310
Eco31I GGTCTC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 45
Eco88I CYCGRG 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 45
EcoT38I GRGCYC 2 cut(s) 95, 310
FaeI CATG 2 cut(s) 139, 193
FaiI YATR 5 cut(s) 126, 137, 191, 210, 255
FaqI GGGAC 1 cut(s) 58
FatI CATG 2 cut(s) 135, 189
FauI CCCGC 1 cut(s) 82
Fnu4HI GCNGC 3 cut(s) 12, 101, 261
FriOI GRGCYC 2 cut(s) 95, 310
Fsp4HI GCNGC 3 cut(s) 12, 101, 261
GluI GCNGC 3 cut(s) 12, 101, 261
GsaI CCCAGC 2 cut(s) 11, 313
HaeIII GGCC 3 cut(s) 77, 260, 269
HapII CCGG 2 cut(s) 169, 270
HgaI GACGC 1 cut(s) 117
Hin1II CATG 2 cut(s) 139, 193
HinfI GANTC 2 cut(s) 80, 296
HpaII CCGG 2 cut(s) 169, 270
HphI GGTGA 1 cut(s) 214
Hpy188I TCNGA 4 cut(s) 30, 37, 145, 286
Hpy188III TCNNGA 1 cut(s) 184
Hpy99I CGWCG 1 cut(s) 133
HpyAV CCTTC 3 cut(s) 75, 149, 214
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 3 cut(s) 72, 94, 144
Hsp92II CATG 2 cut(s) 139, 193
KflI GGGWCCC 1 cut(s) 45
Kzo9I GATC 3 cut(s) 3, 32, 50
LmnI GCTCC 1 cut(s) 171
LpnPI CCDG 6 cut(s) 21, 59, 117, 122, 182, 283
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 279
MalI GATC 3 cut(s) 5, 34, 52
MboI GATC 3 cut(s) 3, 32, 50
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 2 cut(s) 95, 310
MluCI AATT 2 cut(s) 55, 247
MlyI GAGTC 2 cut(s) 89, 305
MnlI CCTC 4 cut(s) 132, 139, 198, 226
MseI TTAA 1 cut(s) 240
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 2 cut(s) 169, 270
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 1 cut(s) 266
NdeII GATC 3 cut(s) 3, 32, 50
NlaIII CATG 2 cut(s) 139, 193
NlaIV GGNNCC 2 cut(s) 46, 47
NmeAIII GCCGAG 2 cut(s) 40, 103
PaeR7I CTCGAG 1 cut(s) 293
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PkrI GCNGC 3 cut(s) 13, 102, 262
PleI GAGTC 2 cut(s) 88, 304
PpsI GAGTC 2 cut(s) 88, 304
PpuMI RGGWCCY 1 cut(s) 45
PsiI TTATAA 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 45
PspFI CCCAGC 2 cut(s) 7, 309
PspN4I GGNNCC 2 cut(s) 46, 47
PspPI GGNCC 1 cut(s) 45
PspPPI RGGWCCY 1 cut(s) 45
PspXI VCTCGAGB 1 cut(s) 293
PsuI RGATCY 1 cut(s) 32
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 3 cut(s) 12, 101, 261
Sau3AI GATC 3 cut(s) 3, 32, 50
Sau96I GGNCC 1 cut(s) 45
ScaI AGTACT 1 cut(s) 163
SchI GAGTC 2 cut(s) 89, 305
SduI GDGCHC 2 cut(s) 95, 310
SetI ASST 2 cut(s) 143, 315
SfaNI GCATC 1 cut(s) 279
SfiI GGCCNNNNNGGCC 1 cut(s) 266
Sfr274I CTCGAG 1 cut(s) 293
SinI GGWCC 1 cut(s) 45
SlaI CTCGAG 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 2 cut(s) 55, 247
SsiI CCGC 3 cut(s) 89, 101, 261
TaqI TCGA 5 cut(s) 60, 119, 131, 185, 294
TasI AATT 2 cut(s) 55, 247
TatI WGTACW 1 cut(s) 161
TauI GCSGC 2 cut(s) 103, 263
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 1 cut(s) 102
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 2 cut(s) 192, 206
TspRI CASTG 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 45
XapI RAATTY 1 cut(s) 247
XhoI CTCGAG 1 cut(s) 293
ZrmI AGTACT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.