Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002272

Physical Location & Seq

Genomic Coordinates Forward (+)

60897635 .. 60901896

Gene Structure 4262 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020089

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGGATCAAATATCTGAGTTGGTGGAACCCCTTATGGGGACACAGCCAGTCGCTGCTTTTGATTTTAACAACTCGGCTTATTTCGGAGGCACAAGAAGGTCCCGAAAGAAGTTTGTGACTTTTGTTGATGATTCCTTGGAAAGAAACCTCCCTGAGCATGAAGACCAGAAAAAGTTCGTTCAGTTATCGTTGGCTATTGCAACCCTTGACAACAAGCTGAAAGACTCTGATGTTGTCGACCGCTGGATAAAGATGGTTACCAAACACTATGTGACAGAGTTAAAACTCCGGATCAAAAACGCACCAAAGATTAGGTACCAATTTCCCCCGGCTGCTTTTGATGTTGGGCAATTAGTTGTACTGGAGCTAATGCATTGTACTTTGGAGAAAGCCTTTATTCAAGAGGATACTATGTTTCTTCCAATTCTTCGGTTAAAAGAGCTCAGATTCAGTCATGTCAATTTCAATGGGGTAGTCGTCGGGGAGCTGCTGTCTAGATGCCCTTTGCTTGAGACTTTGGCATTACGCTCCTGTGGAAACATAAAACATCTCGAACTTTGTTATTTTCCAAATCTCAAGAATGTTGAACTTTGGAATGATTCAGATGATGATGATGATATTTTTACCTACAAGATTGACTCACCCGATCTTGAATCTCTTCTTTATTTGGAGTATGGAAAGTCAATGGACATCGAAGCAGTCACTTGCAAAAATTTAAAGACGCTGCATATAGTTACTCATGGCTCTACCATTACGGACCAATATATCAAGGAAGTCAGTTCCAAATTTCCTTTTCTTGAAACCTTGATACTGAATGCCAGATCTTTTGGATCAAAAGATGCTGATGAGATTAGTATTTCGGGTCATAACCTCAATTCATTGCTAGTTTGTGGACATATTTGCAAACATATGATCATCAATATAGATGCACCAAATCTTCTTAGTTACCAACGCGAGGATGACGAAATTCCTCCCTCGTTTTCTTTAAATTCTGTGAAGCTAGAATGGGCTAACTTTATTGTACCTGGAGCTCATCTCAACAGTGCATGGTTTATTAAATTGCGAGAATATCTGGGAAAGTTTAAGGGTTGTGATCATCTTCGCTTCAGAATATGTCATAAGCAGCCTTGCAAGACAGTCAAATTTTCTTTTGACGAATTACAAGAAGAAAAACTTCCTCCTCTGCCAAAAGTCAGGCAATTGGTTCTGAAAAATGTTCCACCATCGAGAGACTATACAACTCTTATCGATGGCTTACTTTGGAGTTGCCATCCACTCACCATATCAGTACAAGCACGCGGTGCACCTAACCGTAAATTTATAAAGTTCTTACACAAGATGTTAACGGATAGGGAAGAGCAACCCCTCTGCTGCAAAGAATGCCATGTCAAATGTTGGCGGCACTCCTTATCTTCTGTTAAGATTAAGAATCTCTCTGACGCTGAAGAAGAAGAAGAAGAAGAAGAAGATGGCGATGAGGAAGAAGAGGATGACGGTGATGAGGATGAGGATGAGAATGAGGATGAGGATGAGGATGAAGATGAAGATGAGGATGAGGATGAGGATGAGGATGAGGATGAGGATGAAGATGAAGATGAGAATGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAAGATGAAGATGAAGATGAAGATGAGGATGAGGATGAGGGTGAAGAAGAGGGAGATGAAGATGAAGATGAAGAAGAGGGAGCTGAGGAGGGAGATGGACCTGGAGACGGAGATGATGATGATGGATTGGATGATCCAGAATTATTGAAAACATTGAGGGGTCTCGAAAAGAATCATGTTTTTGAGTTACAATGGTCGACGTTTGAGGAGAATGGAGAACTAGATAATTTCGAAATTGTGTTTGACCGAAACGACGACGTAAATGAGGACAGTGAAGAAGACGACGACGATGAAATTGGGTATAGTAGTGACGAAGGCAGTTTATCGAACGGTGGTGGTGGGTCGAAAAGAGAGTACACGGCGGAGGAGATGGAGGCGGAGGCAGCCGGTATTGGGTACAAAGTGGTTGGGCCGCTTGACAACACTGATGGGCTTTTCATTCAGATTGGTTCGCATCAGTTCAAAGTTAGTAATGGGGACTCCATTTTCACCGAAAGATTGAAATTTTGTCAGGTCAATGATAAGTTGATATTGAATAAGGTTCTCTTGGTGGGCACAAGTAGTCAGACAATGATCGGCAGGCCAATAGTTCCAGATGCAGCAGTTCACGCAGTTGTTGAAGAGCATGAAAGTGCATTAGATGCAAAGGTACTTATTTTTAAGAAAAAGAGGAGAAAGAATTACCGTCGGACCAAAGGTCATCGCCAGGAATTAACCAAGTTGAGGACTATTGATATTCAAGGGATTCAGAAACCAGAACCTGTAGTCACCGAAAAACCTCCAAAGGCATCTGGTAAGCAGCCAGAAAAGGTTGCAGTTGCCGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box

Protein Analysis

822

Amino Acids

93.64

Weight (kDa)

4.4

Isoelectric Point (pI)

51.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_At1g61320_AtMIF1	PF23622	63 - 274	1.8e-14	At1g61320/AtMIF1, LRR domain
LRR_At5g56370	PF24758	79 - 248	3.9e-16	FBD-associated F-box protein At5g56370, LRR repeats
Ribosomal_L21p	PF00829	693 - 790	2.3e-25	Ribosomal prokaryotic L21 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002272)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g26980 FvH4_6g32452 FvH4_6g32461 FvH4_6g32741
prunus_persica	Prupe.3G054600_v2.0.a1 Prupe.3G054700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0395301
rosa_laevigata	RLG00000020089 RLG00000020183
rosa_multiflora	Rmu_sc0001483.1_g000030 Rmu_sc0002727.1_g000001 Rmu_sc0005789.1_g000002 Rmu_sc0010454.1_g000003 Rmu_sc0010817.1_g000032 Rmu_sc0035624.1_g000001
rosa_roxburghii	Rroxscaffold_152G00434870 Rroxscaffold_2G00100290 Rroxscaffold_2G00103790 Rroxscaffold_2G00104560
rosa_rugosa	Rorug03G0358200
rosa_samantha	Rh2AG420800 Rh2AG439900 Rh2AG449200 Rh2BG425500 Rh2BG430600 Rh2BG449900 Rh2BG461600 Rh2CG407800 Rh2CG426900 Rh2CG436100 Rh2DG440500 Rh2DG459800 Rh2DG470800 Rh4AG058300 Rh4BG057100 Rh4CG062900 Rh4DG053800 Rh6CG063800 Rh6CG064600 Rh6DG060900 Rh6DG061600
rosa_wichuraiana	Rw2G035990 Rw4G004690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1322
Acc65I	GGTACC	1 cut(s)	315
AccB1I	GGYRCC	1 cut(s)	315
AccI	GTMKAC	2 cut(s)	237, 1835
AccII	CGCG	2 cut(s)	954, 1299
AccIII	TCCGGA	1 cut(s)	288
AciI	CCGC	7 cut(s)	241, 1299, 1399, 2000, 2015, 2051, 2460
AclWI	GGATC	4 cut(s)	12, 299, 838, 1766
AcsI	RAATTY	7 cut(s)	712, 785, 966, 988, 1142, 1316, 2141
AcuI	CTGAAG	2 cut(s)	1090, 1464
AdeI	CACNNNGTG	2 cut(s)	271, 1301
AfaI	GTAC	8 cut(s)	317, 360, 379, 1023, 1290, 1994, 2036, 2289
AfiI	CCNNNNNNNGG	6 cut(s)	35, 36, 955, 1745, 2031, 2361
AhdI	GACNNNNNGTC	1 cut(s)	2334
AjnI	CCWGG	3 cut(s)	1024, 1738, 2343
AluBI	AGCT	7 cut(s)	217, 367, 442, 487, 1000, 1031, 1721
AluI	AGCT	7 cut(s)	217, 367, 442, 487, 1000, 1031, 1721
Alw21I	GWGCWC	3 cut(s)	444, 1033, 1306
Alw26I	GTCTC	4 cut(s)	506, 1224, 1737, 1805
Alw44I	GTGCAC	1 cut(s)	1302
AlwI	GGATC	4 cut(s)	12, 299, 838, 1766
AlwNI	CAGNNNCTG	2 cut(s)	53, 2399
Aor13HI	TCCGGA	1 cut(s)	288
AoxI	GGCC	2 cut(s)	2048, 2219
ApaLI	GTGCAC	1 cut(s)	1302
ApeKI	GCWGC	9 cut(s)	53, 332, 487, 724, 1123, 1371, 2021, 2237, 2437
ApoI	RAATTY	7 cut(s)	712, 785, 966, 988, 1142, 1316, 2141
Asp700I	GAANNNNTTC	3 cut(s)	173, 657, 1173
Asp718I	GGTACC	1 cut(s)	315
AspS9I	GGNCC	5 cut(s)	99, 757, 1736, 2048, 2328
AsuC2I	CCSGG	1 cut(s)	329
AsuHPI	GGTGA	6 cut(s)	633, 1270, 1508, 1691, 2119, 2398
AsuII	TTCGAA	1 cut(s)	1869
AvaII	GGWCC	4 cut(s)	99, 757, 1736, 2328
BaeGI	GKGCMC	2 cut(s)	1306, 2195
BanI	GGYRCC	1 cut(s)	315
BanII	GRGCYC	2 cut(s)	444, 1033
BbsI	GAAGAC	2 cut(s)	168, 1923
Bbv12I	GWGCWC	3 cut(s)	444, 1033, 1306
BbvCI	CCTCAGC	1 cut(s)	1722
BbvI	GCAGC	9 cut(s)	40, 319, 474, 711, 1135, 1358, 2033, 2249, 2449
BccI	CCATC	9 cut(s)	247, 1231, 1244, 1278, 1463, 1727, 1754, 2002, 2060
BceAI	ACGGC	1 cut(s)	2013
BciT130I	CCWGG	3 cut(s)	1026, 1740, 2345
BciVI	GTATCC	1 cut(s)	400
BclI	TGATCA	2 cut(s)	912, 1093
BcnI	CCSGG	1 cut(s)	329
BcoDI	GTCTC	4 cut(s)	506, 1224, 1737, 1805
BfaI	CTAG	4 cut(s)	495, 884, 1001, 1859
BfmI	CTRYAG	1 cut(s)	2400
BfuI	GTATCC	1 cut(s)	400
BglII	AGATCT	1 cut(s)	821
Bme1390I	CCNGG	4 cut(s)	329, 1026, 1740, 2345
Bme18I	GGWCC	4 cut(s)	99, 757, 1736, 2328
BmeRI	GACNNNNNGTC	1 cut(s)	2334
BmgT120I	GGNCC	5 cut(s)	99, 757, 1736, 2048, 2328
BmiI	GGNNCC	3 cut(s)	27, 101, 317
BmrFI	CCNGG	4 cut(s)	329, 1026, 1740, 2345
BmsI	GCATC	7 cut(s)	488, 829, 916, 2101, 2224, 2269, 2435
BpiI	GAAGAC	2 cut(s)	168, 1923
BplI	GAGNNNNNCTC	2 cut(s)	1020, 1052
BpmI	CTGGAG	3 cut(s)	383, 1047, 1761
Bpu10I	CCTNAGC	2 cut(s)	153, 1722
Bpu14I	TTCGAA	1 cut(s)	1869
BpuEI	CTTGAG	2 cut(s)	530, 560
BpuMI	CCSGG	1 cut(s)	329
Bsa29I	ATCGAT	1 cut(s)	1248
BsaI	GGTCTC	1 cut(s)	1805
BsaJI	CCNNGG	2 cut(s)	135, 327
BsaWI	WCCGGW	1 cut(s)	288
BsaXI	ACNNNNNCTCC	2 cut(s)	476, 506
Bsc4I	CCNNNNNNNGG	6 cut(s)	35, 36, 955, 1745, 2031, 2361
Bse118I	RCCGGY	1 cut(s)	2024
Bse1I	ACTGG	2 cut(s)	47, 366
Bse3DI	GCAATG	1 cut(s)	878
BseAI	TCCGGA	1 cut(s)	288
BseBI	CCWGG	3 cut(s)	1026, 1740, 2345
BseCI	ATCGAT	1 cut(s)	1248
BseDI	CCNNGG	2 cut(s)	135, 327
BseLI	CCNNNNNNNGG	6 cut(s)	35, 36, 955, 1745, 2031, 2361
BseMI	GCAATG	1 cut(s)	878
BseMII	CTCAG	4 cut(s)	6, 144, 457, 1713
BseNI	ACTGG	2 cut(s)	47, 366
BseRI	GAGGAG	5 cut(s)	1170, 1739, 1859, 2018, 2323
BseSI	GKGCMC	2 cut(s)	1306, 2195
BseXI	GCAGC	9 cut(s)	40, 319, 474, 711, 1135, 1358, 2033, 2249, 2449
Bsh1236I	CGCG	2 cut(s)	954, 1299
Bsh1285I	CGRYCG	1 cut(s)	241
BshFI	GGCC	2 cut(s)	2050, 2221
BshNI	GGYRCC	1 cut(s)	315
BshVI	ATCGAT	1 cut(s)	1248
BsiEI	CGRYCG	1 cut(s)	241
BsiHKAI	GWGCWC	3 cut(s)	444, 1033, 1306
BsiSI	CCGG	3 cut(s)	289, 329, 2025
BslFI	GGGAC	3 cut(s)	52, 85, 2129
BslI	CCNNNNNNNGG	6 cut(s)	35, 36, 955, 1745, 2031, 2361
BsmAI	GTCTC	4 cut(s)	506, 1224, 1737, 1805
BsmBI	CGTCTC	1 cut(s)	1737
BsmFI	GGGAC	3 cut(s)	52, 85, 2129
BsmI	GAATGC	2 cut(s)	820, 1385
BsnI	GGCC	2 cut(s)	2050, 2221
Bso31I	GGTCTC	1 cut(s)	1805
Bsp119I	TTCGAA	1 cut(s)	1869
Bsp1286I	GDGCHC	4 cut(s)	444, 1033, 1306, 2195
Bsp13I	TCCGGA	1 cut(s)	288
Bsp143I	GATC	9 cut(s)	4, 291, 646, 821, 830, 912, 1093, 1771, 2211
BspACI	CCGC	7 cut(s)	241, 1299, 1399, 2000, 2015, 2051, 2460
BspANI	GGCC	2 cut(s)	2050, 2221
BspCNI	CTCAG	4 cut(s)	7, 145, 456, 1714
BspDI	ATCGAT	1 cut(s)	1248
BspEI	TCCGGA	1 cut(s)	288
BspFNI	CGCG	2 cut(s)	954, 1299
BspLI	GGNNCC	3 cut(s)	27, 101, 317
BspPI	GGATC	4 cut(s)	12, 299, 838, 1766
BspQI	GCTCTTC	2 cut(s)	1350, 2253
BspT104I	TTCGAA	1 cut(s)	1869
BspT107I	GGYRCC	1 cut(s)	315
BspTNI	GGTCTC	1 cut(s)	1805
BsrDI	GCAATG	1 cut(s)	878
BsrFI	RCCGGY	1 cut(s)	2024
BsrI	ACTGG	2 cut(s)	47, 366
BssAI	RCCGGY	1 cut(s)	2024
BssECI	CCNNGG	2 cut(s)	135, 327
BssMI	GATC	9 cut(s)	4, 291, 646, 821, 830, 912, 1093, 1771, 2211
BssT1I	CCWWGG	1 cut(s)	135
Bst2UI	CCWGG	3 cut(s)	1026, 1740, 2345
Bst4CI	ACNGT	7 cut(s)	1043, 1138, 1313, 1496, 1910, 1970, 2324
Bst6I	CTCTTC	6 cut(s)	663, 1350, 1479, 1680, 1707, 2253
BstAPI	GCANNNNNTGC	3 cut(s)	1301, 1380, 2279
BstBI	TTCGAA	1 cut(s)	1869
BstC8I	GCNNGC	2 cut(s)	1297, 2219
BstDEI	CTNAG	5 cut(s)	15, 153, 443, 941, 1722
BstEII	GGTNACC	1 cut(s)	256
BstFNI	CGCG	2 cut(s)	954, 1299
BstKTI	GATC	9 cut(s)	7, 294, 649, 824, 833, 915, 1096, 1774, 2214
BstMAI	GTCTC	4 cut(s)	506, 1224, 1737, 1805
BstMBI	GATC	9 cut(s)	4, 291, 646, 821, 830, 912, 1093, 1771, 2211
BstMCI	CGRYCG	1 cut(s)	241
BstMWI	GCNNNNNNNGC	6 cut(s)	1301, 1380, 2021, 2246, 2279, 2459
BstNI	CCWGG	3 cut(s)	1026, 1740, 2345
BstPI	GGTNACC	1 cut(s)	256
BstSCI	CCNGG	4 cut(s)	327, 1024, 1738, 2343
BstSFI	CTRYAG	1 cut(s)	2400
BstSLI	GKGCMC	2 cut(s)	1306, 2195
BstUI	CGCG	2 cut(s)	954, 1299
BstV1I	GCAGC	9 cut(s)	40, 319, 474, 711, 1135, 1358, 2033, 2249, 2449
BstV2I	GAAGAC	2 cut(s)	168, 1923
BstX2I	RGATCY	1 cut(s)	821
BstYI	RGATCY	1 cut(s)	821
Bsu15I	ATCGAT	1 cut(s)	1248
BsuI	GTATCC	1 cut(s)	400
BsuRI	GGCC	2 cut(s)	2050, 2221
BsuTUI	ATCGAT	1 cut(s)	1248
BtgZI	GCGATG	2 cut(s)	1488, 2324
BtsIMutI	CAGTG	3 cut(s)	1048, 1915, 2061
Cac8I	GCNNGC	2 cut(s)	1297, 2219
CaiI	CAGNNNCTG	2 cut(s)	53, 2399
Cfr10I	RCCGGY	1 cut(s)	2024
Cfr13I	GGNCC	5 cut(s)	99, 757, 1736, 2048, 2328
ClaI	ATCGAT	1 cut(s)	1248
CseI	GACGC	2 cut(s)	730, 1448
Csp6I	GTAC	8 cut(s)	316, 359, 378, 1022, 1289, 1993, 2035, 2288
CviAII	CATG	7 cut(s)	158, 455, 740, 1047, 1385, 1814, 2264
CviQI	GTAC	8 cut(s)	316, 359, 378, 1022, 1289, 1993, 2035, 2288
DdeI	CTNAG	5 cut(s)	15, 153, 443, 941, 1722
DpnI	GATC	9 cut(s)	6, 293, 648, 823, 832, 914, 1095, 1773, 2213
DpnII	GATC	9 cut(s)	4, 291, 646, 821, 830, 912, 1093, 1771, 2211
DraI	TTTAAA	2 cut(s)	717, 987
DraIII	CACNNNGTG	2 cut(s)	271, 1301
DriI	GACNNNNNGTC	1 cut(s)	2334
Eam1104I	CTCTTC	6 cut(s)	663, 1350, 1479, 1680, 1707, 2253
Eam1105I	GACNNNNNGTC	1 cut(s)	2334
EarI	CTCTTC	6 cut(s)	663, 1350, 1479, 1680, 1707, 2253
EciI	GGCGGA	2 cut(s)	2015, 2030
Ecl136II	GAGCTC	2 cut(s)	442, 1031
Eco130I	CCWWGG	1 cut(s)	135
Eco24I	GRGCYC	2 cut(s)	444, 1033
Eco31I	GGTCTC	1 cut(s)	1805
Eco47I	GGWCC	4 cut(s)	99, 757, 1736, 2328
Eco53kI	GAGCTC	2 cut(s)	442, 1031
Eco57I	CTGAAG	2 cut(s)	1090, 1464
Eco91I	GGTNACC	1 cut(s)	256
EcoICRI	GAGCTC	2 cut(s)	442, 1031
EcoO109I	RGGNCCY	1 cut(s)	99
EcoO65I	GGTNACC	1 cut(s)	256
EcoRII	CCWGG	3 cut(s)	1024, 1738, 2343
EcoT14I	CCWWGG	1 cut(s)	135
EcoT22I	ATGCAT	1 cut(s)	375
EcoT38I	GRGCYC	2 cut(s)	444, 1033
ErhI	CCWWGG	1 cut(s)	135
Esp3I	CGTCTC	1 cut(s)	1737
FaeI	CATG	7 cut(s)	161, 458, 743, 1050, 1388, 1817, 2267
FalI	AAGNNNNNCTT	4 cut(s)	1158, 1190, 2168, 2200
FaqI	GGGAC	3 cut(s)	52, 85, 2129
FatI	CATG	7 cut(s)	157, 454, 739, 1046, 1384, 1813, 2263
FauNDI	CATATG	1 cut(s)	909
FbaI	TGATCA	2 cut(s)	912, 1093
FblI	GTMKAC	2 cut(s)	237, 1835
FriOI	GRGCYC	2 cut(s)	444, 1033
FspBI	CTAG	4 cut(s)	495, 884, 1001, 1859
GsuI	CTGGAG	3 cut(s)	383, 1047, 1761
HaeIII	GGCC	2 cut(s)	2050, 2221
HapII	CCGG	3 cut(s)	289, 329, 2025
HgaI	GACGC	2 cut(s)	730, 1448
Hin1II	CATG	7 cut(s)	161, 458, 743, 1050, 1388, 1817, 2267
HincII	GTYRAC	3 cut(s)	238, 1344, 1836
HindII	GTYRAC	3 cut(s)	238, 1344, 1836
HpaI	GTTAAC	1 cut(s)	1344
HpaII	CCGG	3 cut(s)	289, 329, 2025
HphI	GGTGA	6 cut(s)	633, 1270, 1508, 1691, 2119, 2398
Hpy166II	GTNNAC	7 cut(s)	238, 893, 1304, 1344, 1836, 1995, 2245
Hpy8I	GTNNAC	7 cut(s)	238, 893, 1304, 1344, 1836, 1995, 2245
Hpy99I	CGWCG	7 cut(s)	482, 1840, 1895, 1898, 1925, 1928, 2328
HpyAV	CCTTC	2 cut(s)	90, 1946
HpyCH4III	ACNGT	7 cut(s)	1043, 1138, 1313, 1496, 1910, 1970, 2324
HpyCH4IV	ACGT	2 cut(s)	1838, 1896
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1301, 1380, 2021, 2246, 2279, 2459
HpyF3I	CTNAG	5 cut(s)	15, 153, 443, 941, 1722
HpySE526I	ACGT	2 cut(s)	1838, 1896
Hsp92II	CATG	7 cut(s)	161, 458, 743, 1050, 1388, 1817, 2267
Kpn2I	TCCGGA	1 cut(s)	288
KpnI	GGTACC	1 cut(s)	319
Ksp22I	TGATCA	2 cut(s)	912, 1093
KspAI	GTTAAC	1 cut(s)	1344
Kzo9I	GATC	9 cut(s)	4, 291, 646, 821, 830, 912, 1093, 1771, 2211
LguI	GCTCTTC	2 cut(s)	1350, 2253
LmnI	GCTCC	5 cut(s)	364, 484, 533, 1028, 1718
Lsp1109I	GCAGC	9 cut(s)	40, 319, 474, 711, 1135, 1358, 2033, 2249, 2449
LweI	GCATC	7 cut(s)	488, 829, 916, 2101, 2224, 2269, 2435
MaeI	CTAG	4 cut(s)	495, 884, 1001, 1859
MaeII	ACGT	2 cut(s)	1838, 1896
MaeIII	GTNAC	9 cut(s)	115, 256, 271, 700, 733, 944, 1824, 1946, 2404
MalI	GATC	9 cut(s)	6, 293, 648, 823, 832, 914, 1095, 1773, 2213
MboI	GATC	9 cut(s)	4, 291, 646, 821, 830, 912, 1093, 1771, 2211
MfeI	CAATTG	1 cut(s)	1199
MflI	RGATCY	1 cut(s)	821
MhlI	GDGCHC	4 cut(s)	444, 1033, 1306, 2195
MlyI	GAGTC	3 cut(s)	218, 632, 2111
MmeI	TCCRAC	1 cut(s)	2306
Mph1103I	ATGCAT	1 cut(s)	375
MroI	TCCGGA	1 cut(s)	288
MroXI	GAANNNNTTC	3 cut(s)	173, 657, 1173
MslI	CAYNNNNRTG	1 cut(s)	2268
MspA1I	CMGCKG	1 cut(s)	243
MspI	CCGG	3 cut(s)	289, 329, 2025
MspR9I	CCNGG	4 cut(s)	329, 1026, 1740, 2345
MunI	CAATTG	1 cut(s)	1199
Mva1269I	GAATGC	2 cut(s)	820, 1385
MvaI	CCWGG	3 cut(s)	1026, 1740, 2345
MvnI	CGCG	2 cut(s)	954, 1299
MwoI	GCNNNNNNNGC	6 cut(s)	1301, 1380, 2021, 2246, 2279, 2459
NciI	CCSGG	1 cut(s)	329
NdeI	CATATG	1 cut(s)	909
NdeII	GATC	9 cut(s)	4, 291, 646, 821, 830, 912, 1093, 1771, 2211
NlaIII	CATG	7 cut(s)	161, 458, 743, 1050, 1388, 1817, 2267
NlaIV	GGNNCC	3 cut(s)	27, 101, 317
NmeAIII	GCCGAG	1 cut(s)	53
NmuCI	GTSAC	5 cut(s)	115, 271, 700, 1946, 2404
NsiI	ATGCAT	1 cut(s)	375
NspV	TTCGAA	1 cut(s)	1869
PciSI	GCTCTTC	2 cut(s)	1350, 2253
PctI	GAATGC	2 cut(s)	820, 1385
PdmI	GAANNNNTTC	3 cut(s)	173, 657, 1173
PfeI	GAWTC	7 cut(s)	131, 447, 599, 653, 1429, 1810, 2383
PleI	GAGTC	3 cut(s)	218, 632, 2111
PpsI	GAGTC	3 cut(s)	218, 632, 2111
PpuMI	RGGWCCY	1 cut(s)	99
PsiI	TTATAA	1 cut(s)	1322
Psp124BI	GAGCTC	2 cut(s)	444, 1033
Psp5II	RGGWCCY	1 cut(s)	99
Psp6I	CCWGG	3 cut(s)	1024, 1738, 2343
PspEI	GGTNACC	1 cut(s)	256
PspGI	CCWGG	3 cut(s)	1024, 1738, 2343
PspN4I	GGNNCC	3 cut(s)	27, 101, 317
PspPI	GGNCC	5 cut(s)	99, 757, 1736, 2048, 2328
PspPPI	RGGWCCY	1 cut(s)	99
PstNI	CAGNNNCTG	2 cut(s)	53, 2399
PsuI	RGATCY	1 cut(s)	821
RsaI	GTAC	8 cut(s)	317, 360, 379, 1023, 1290, 1994, 2036, 2289
RsaNI	GTAC	8 cut(s)	316, 359, 378, 1022, 1289, 1993, 2035, 2288
RseI	CAYNNNNRTG	1 cut(s)	2268
SacI	GAGCTC	2 cut(s)	444, 1033
SalI	GTCGAC	2 cut(s)	236, 1834
SapI	GCTCTTC	2 cut(s)	1350, 2253
Sau3AI	GATC	9 cut(s)	4, 291, 646, 821, 830, 912, 1093, 1771, 2211
Sau96I	GGNCC	5 cut(s)	99, 757, 1736, 2048, 2328
SchI	GAGTC	3 cut(s)	218, 632, 2111
ScrFI	CCNGG	4 cut(s)	329, 1026, 1740, 2345
SduI	GDGCHC	4 cut(s)	444, 1033, 1306, 2195
SfaNI	GCATC	7 cut(s)	488, 829, 916, 2101, 2224, 2269, 2435
SfcI	CTRYAG	1 cut(s)	2400
SfuI	TTCGAA	1 cut(s)	1869
SinI	GGWCC	4 cut(s)	99, 757, 1736, 2328
SmiMI	CAYNNNNRTG	1 cut(s)	2268
SmlI	CTYRAG	2 cut(s)	509, 575
SmoI	CTYRAG	2 cut(s)	509, 575
SsiI	CCGC	7 cut(s)	241, 1299, 1399, 2000, 2015, 2051, 2460
SspMI	CTAG	4 cut(s)	495, 884, 1001, 1859
SstI	GAGCTC	2 cut(s)	444, 1033
StyD4I	CCNGG	4 cut(s)	327, 1024, 1738, 2343
StyI	CCWWGG	1 cut(s)	135
TaaI	ACNGT	7 cut(s)	1043, 1138, 1313, 1496, 1910, 1970, 2324
TaiI	ACGT	2 cut(s)	1841, 1899
TaqII	GACCGA	1 cut(s)	1899
TatI	WGTACW	4 cut(s)	358, 377, 1288, 1992
TauI	GCSGC	3 cut(s)	1402, 2053, 2462
TfiI	GAWTC	7 cut(s)	131, 447, 599, 653, 1429, 1810, 2383
TscAI	CASTG	3 cut(s)	1048, 1915, 2068
TseFI	GTSAC	5 cut(s)	115, 271, 700, 1946, 2404
TseI	GCWGC	9 cut(s)	53, 332, 487, 724, 1123, 1371, 2021, 2237, 2437
Tsp45I	GTSAC	5 cut(s)	115, 271, 700, 1946, 2404
TspGWI	ACGGA	3 cut(s)	770, 1361, 1761
TspRI	CASTG	3 cut(s)	1048, 1915, 2068
VneI	GTGCAC	1 cut(s)	1302
VpaK11BI	GGWCC	4 cut(s)	99, 757, 1736, 2328
XapI	RAATTY	7 cut(s)	712, 785, 966, 988, 1142, 1316, 2141
XbaI	TCTAGA	1 cut(s)	494
XmiI	GTMKAC	2 cut(s)	237, 1835
XmnI	GAANNNNTTC	3 cut(s)	173, 657, 1173
XspI	CTAG	4 cut(s)	495, 884, 1001, 1859
Zsp2I	ATGCAT	1 cut(s)	375

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.