RLG00000020183

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002272
Physical Location & Seq
Reverse (-)
62169248 .. 62175231
5984 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020183

Sequence Viewer

Length: 1524 bp
ATGGCCAAATCCACACAACAACCAGTTGGCCCTCCAGTTGCTTCAATAATTAATGAGGCATCATTGATATCCCATACCCCATATTCGACCAAAAAGACCCTCCACTATATCCCTAACTCAATCATTGATAATACATCCCCATTTTTCAGAAAAGTGAAACAGGGTATTACCGATTTGAAACTTCGTCTACAGAATTCTCGCTATCATGTTCCCCTGCCGTATGCTTATTCTTCTTACAGTAATGAAAGTGGAGCTTCTCAACCTCTTTCAAAGCTAGCTTTAGATCACGAGATATCGACGCCATGGATCGGATATAGCAGTTGCCGAAGCCCTTATGCTGGAAATCCTCCTCCGGTTGCCTCCGATAGATTCAGTCCGTCTGAAAATCACGTCGAGGACATGGAACTCACTCCCGGGGAGCGAAAGAGGTTGAACTGCCTAAAAGAGCTGCGTTTGCATGTTGATTTAACCAGATTATCAGTAGTGGATGTGCTTTCTATGTGTCCTTCCCTTGAGATCTTAGATCTCAGATACTGTTACAACATAAACTATCTTCGACTCTGTGACTTTCCTAAGCTAAAGCAGGTTTACCTTACACCAAGATACTATAGGGACATTTATAGTTACAAGATTGAAGCAGCCAATCTCGAAACTCTCCATCTTGAAACTTATCACTCAAACTGTCAATGTTACCCGACTTGTCAATGCTCCCAGCCAGTGGACCTTGAAGTAGTCAGGTGCAGTAATCTAATGGAGTTGATCGTTATGACTCCCGGTAGTACCATAACCCGCCAATATGTTGAAAAACTTACTCGTAAGTTTCCCCATCTTGAGGTGTTAAGCTTAGAAGCCTCTTTCGGATCACCCAAGCTCAACAAAAAAATCCACCTCCGGCGGTTTTTGTTGGATTCTACCAAGCTTGAAGAACTGAATGTTTTTCTAGATAAGAGCGATTTTCCTAAAGATCTTGACTTCTTTATTACTTTGCAAAAGAGATTTCGAGAATTCGCTTCACAAGAACGCTCGACGGTTTTTATAAACGGTTCAGAAATCAAATTTTGTCTGAAACTATTAAGAGAAGAACCATCAGTACCAGAAATTATGCAGTTACAGTTGAAACATAGAGATGATTTTACACGGGAAGACCTTGGCTATTGCTTTTCAACTTTACTTGATAACTTGCTGTGGAGTTGTCATCCAATGGCCTTATCCATCACAGAAAATGGATGGATTTTAAAGGCCATCTGCAAGAAACTCATGTCTTGGGAAGAGAATTCACCATGTTGTAGAGACAACAGCATCAAATGTTGGCGGCACTATTTAAAGTCTATTGAGATTCAAAACATGAATGGTCGTGATGCAGATCTATCGATACAAGCAATACATATCGAAGGCGTTGACAGTATCACTTTTGCACTACAATGGGCCATTGAGCTTTGTGACGAGTTAGGACTGTTATGGAGCATCGAAGATGGCCTAATGGTTCGCCGTGGAGGGGCAGTGATGCGTAATATAGATGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

508

Amino Acids

58.38

Weight (kDa)

6.71

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 140 - 290 3.8e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1037
Acc36I ACCTGC 1 cut(s) 574
AccB7I CCANNNNNTGG 1 cut(s) 718
AccI GTMKAC 1 cut(s) 187
AciI CCGC 3 cut(s) 790, 895, 1312
AclWI GGATC 2 cut(s) 314, 868
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 193, 1004, 1055, 1273
AcyI GRCGYC 1 cut(s) 299
AfaI GTAC 2 cut(s) 781, 1092
AfiI CCNNNNNNNGG 5 cut(s) 308, 338, 718, 832, 1495
AjiI CACGTC 1 cut(s) 391
AluBI AGCT 9 cut(s) 254, 274, 278, 448, 577, 843, 871, 919, 1435
AluI AGCT 9 cut(s) 254, 274, 278, 448, 577, 843, 871, 919, 1435
Alw26I GTCTC 1 cut(s) 1284
AlwI GGATC 2 cut(s) 314, 868
AlwNI CAGNNNCTG 1 cut(s) 534
Ama87I CYCGRG 1 cut(s) 413
AoxI GGCC 6 cut(s) 3, 28, 1203, 1239, 1425, 1474
ApeKI GCWGC 2 cut(s) 448, 638
ApoI RAATTY 4 cut(s) 193, 1004, 1055, 1273
AseI ATTAAT 1 cut(s) 51
AspS9I GGNCC 3 cut(s) 29, 721, 1425
AsuC2I CCSGG 3 cut(s) 414, 415, 774
AsuHPI GGTGA 2 cut(s) 855, 1269
AsuNHI GCTAGC 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 1 cut(s) 721
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 287
BbsI GAAGAC 1 cut(s) 1149
BbvI GCAGC 2 cut(s) 435, 650
BccI CCATC 8 cut(s) 666, 834, 1093, 1220, 1221, 1250, 1466, 1511
BceAI ACGGC 2 cut(s) 202, 1473
BcgI CGANNNNNNTGC 2 cut(s) 1350, 1384
BcnI CCSGG 3 cut(s) 414, 415, 774
BcoDI GTCTC 1 cut(s) 1284
BfaI CTAG 2 cut(s) 275, 941
BfmI CTRYAG 2 cut(s) 188, 607
BfuAI ACCTGC 1 cut(s) 574
BglII AGATCT 4 cut(s) 516, 523, 964, 1363
BisI GCNGC 3 cut(s) 449, 639, 1313
BlsI GCNGC 3 cut(s) 450, 640, 1314
Bme1390I CCNGG 3 cut(s) 414, 415, 774
Bme18I GGWCC 1 cut(s) 721
BmeT110I CYCGRG 1 cut(s) 413
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 3 cut(s) 29, 721, 1425
BmrFI CCNGG 3 cut(s) 414, 415, 774
BmsI GCATC 5 cut(s) 68, 1308, 1348, 1473, 1494
BmtI GCTAGC 1 cut(s) 278
BpiI GAAGAC 1 cut(s) 1149
BpmI CTGGAG 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 573
BpuEI CTTGAG 2 cut(s) 533, 851
BpuMI CCSGG 3 cut(s) 414, 415, 774
Bsa29I ATCGAT 1 cut(s) 1370
BsaBI GATNNNNATC 1 cut(s) 1362
BsaHI GRCGYC 1 cut(s) 299
BsaJI CCNNGG 5 cut(s) 302, 413, 414, 1147, 1489
BsaWI WCCGGW 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 1485, 1515
Bsc4I CCNNNNNNNGG 5 cut(s) 308, 338, 718, 832, 1495
Bse1I ACTGG 3 cut(s) 23, 35, 716
Bse8I GATNNNNATC 1 cut(s) 1362
BseCI ATCGAT 1 cut(s) 1370
BseDI CCNNGG 5 cut(s) 302, 413, 414, 1147, 1489
BseGI GGATG 4 cut(s) 134, 493, 1195, 1232
BseJI GATNNNNATC 1 cut(s) 1362
BseLI CCNNNNNNNGG 5 cut(s) 308, 338, 718, 832, 1495
BseMII CTCAG 1 cut(s) 541
BseNI ACTGG 3 cut(s) 23, 35, 716
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 2 cut(s) 435, 650
BseYI CCCAGC 1 cut(s) 711
BsgI GTGCAG 1 cut(s) 760
BshFI GGCC 6 cut(s) 5, 30, 1205, 1241, 1427, 1476
BshVI ATCGAT 1 cut(s) 1370
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 4 cut(s) 353, 414, 774, 892
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 5 cut(s) 308, 338, 718, 832, 1495
BsmAI GTCTC 1 cut(s) 1284
BsmFI GGGAC 1 cut(s) 626
BsnI GGCC 6 cut(s) 5, 30, 1205, 1241, 1427, 1476
BsoBI CYCGRG 1 cut(s) 413
Bsp143I GATC 8 cut(s) 283, 306, 516, 523, 759, 860, 964, 1363
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 3 cut(s) 790, 895, 1312
BspANI GGCC 6 cut(s) 5, 30, 1205, 1241, 1427, 1476
BspCNI CTCAG 1 cut(s) 540
BspDI ATCGAT 1 cut(s) 1370
BspMI ACCTGC 1 cut(s) 574
BspOI GCTAGC 1 cut(s) 278
BspPI GGATC 2 cut(s) 314, 868
BsrI ACTGG 3 cut(s) 23, 35, 716
BssECI CCNNGG 5 cut(s) 302, 413, 414, 1147, 1489
BssMI GATC 8 cut(s) 283, 306, 516, 523, 759, 860, 964, 1363
BssNI GRCGYC 1 cut(s) 299
BssSI CACGAG 1 cut(s) 287
BssT1I CCWWGG 2 cut(s) 302, 1147
Bst2BI CACGAG 1 cut(s) 287
Bst4CI ACNGT 8 cut(s) 239, 536, 683, 1030, 1043, 1113, 1403, 1455
Bst6I CTCTTC 1 cut(s) 1263
BstACI GRCGYC 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 4 cut(s) 520, 527, 573, 844
BstDSI CCRYGG 2 cut(s) 302, 1489
BstF5I GGATG 4 cut(s) 134, 493, 1195, 1232
BstKTI GATC 8 cut(s) 286, 309, 519, 526, 762, 863, 967, 1366
BstMAI GTCTC 1 cut(s) 1284
BstMBI GATC 8 cut(s) 283, 306, 516, 523, 759, 860, 964, 1363
BstMWI GCNNNNNNNGC 1 cut(s) 454
BstNSI RCATGY 1 cut(s) 461
BstSCI CCNGG 3 cut(s) 412, 413, 772
BstSFI CTRYAG 2 cut(s) 188, 607
BstV1I GCAGC 2 cut(s) 435, 650
BstV2I GAAGAC 1 cut(s) 1149
BstX2I RGATCY 4 cut(s) 516, 523, 964, 1363
BstYI RGATCY 4 cut(s) 516, 523, 964, 1363
Bsu15I ATCGAT 1 cut(s) 1370
BsuRI GGCC 6 cut(s) 5, 30, 1205, 1241, 1427, 1476
BsuTUI ATCGAT 1 cut(s) 1370
BtgI CCRYGG 2 cut(s) 302, 1489
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 4 cut(s) 134, 493, 1195, 1232
BtsI GCAGTG 1 cut(s) 1506
BtsIMutI CAGTG 2 cut(s) 723, 1506
BveI ACCTGC 1 cut(s) 574
Cac8I GCNNGC 1 cut(s) 276
CaiI CAGNNNCTG 1 cut(s) 534
Cfr13I GGNCC 3 cut(s) 29, 721, 1425
Cfr9I CCCGGG 1 cut(s) 413
ClaI ATCGAT 1 cut(s) 1370
CseI GACGC 1 cut(s) 307
Csp6I GTAC 2 cut(s) 780, 1091
CviAII CATG 7 cut(s) 206, 303, 400, 458, 1258, 1281, 1345
CviQI GTAC 2 cut(s) 780, 1091
DdeI CTNAG 4 cut(s) 520, 527, 573, 844
DpnI GATC 8 cut(s) 285, 308, 518, 525, 761, 862, 966, 1365
DpnII GATC 8 cut(s) 283, 306, 516, 523, 759, 860, 964, 1363
DraI TTTAAA 2 cut(s) 1236, 1323
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 1263
EarI CTCTTC 1 cut(s) 1263
Eco130I CCWWGG 2 cut(s) 302, 1147
Eco32I GATATC 2 cut(s) 69, 294
Eco47I GGWCC 1 cut(s) 721
Eco88I CYCGRG 1 cut(s) 413
EcoRI GAATTC 3 cut(s) 193, 1004, 1273
EcoRV GATATC 2 cut(s) 69, 294
EcoT14I CCWWGG 2 cut(s) 302, 1147
ErhI CCWWGG 2 cut(s) 302, 1147
FaeI CATG 7 cut(s) 209, 306, 403, 461, 1261, 1284, 1348
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FaqI GGGAC 1 cut(s) 626
FatI CATG 7 cut(s) 205, 302, 399, 457, 1257, 1280, 1344
FauI CCCGC 1 cut(s) 797
FblI GTMKAC 1 cut(s) 187
Fnu4HI GCNGC 3 cut(s) 449, 639, 1313
FokI GGATG 4 cut(s) 121, 500, 1182, 1239
Fsp4HI GCNGC 3 cut(s) 449, 639, 1313
FspBI CTAG 2 cut(s) 275, 941
GluI GCNGC 3 cut(s) 449, 639, 1313
GsaI CCCAGC 1 cut(s) 715
GsuI CTGGAG 1 cut(s) 18
HaeIII GGCC 6 cut(s) 5, 30, 1205, 1241, 1427, 1476
HapII CCGG 4 cut(s) 353, 414, 774, 892
HgaI GACGC 1 cut(s) 307
Hin1I GRCGYC 1 cut(s) 299
Hin1II CATG 7 cut(s) 209, 306, 403, 461, 1261, 1284, 1348
HincII GTYRAC 1 cut(s) 1399
HindII GTYRAC 1 cut(s) 1399
HindIII AAGCTT 2 cut(s) 841, 917
HinfI GANTC 5 cut(s) 369, 558, 769, 908, 1336
HpaII CCGG 4 cut(s) 353, 414, 774, 892
HphI GGTGA 2 cut(s) 855, 1269
Hpy166II GTNNAC 4 cut(s) 188, 589, 721, 1399
Hpy188I TCNGA 8 cut(s) 149, 311, 364, 382, 530, 860, 1048, 1065
Hpy188III TCNNGA 8 cut(s) 287, 647, 662, 830, 941, 968, 1001, 1355
Hpy8I GTNNAC 4 cut(s) 188, 589, 721, 1399
Hpy99I CGWCG 3 cut(s) 301, 395, 1030
HpyAV CCTTC 2 cut(s) 516, 1385
HpyCH4III ACNGT 8 cut(s) 239, 536, 683, 1030, 1043, 1113, 1403, 1455
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 7 cut(s) 457, 741, 988, 1105, 1248, 1361, 1415
HpyF10VI GCNNNNNNNGC 1 cut(s) 454
HpyF3I CTNAG 4 cut(s) 520, 527, 573, 844
HpySE526I ACGT 1 cut(s) 390
Hsp92I GRCGYC 1 cut(s) 299
Hsp92II CATG 7 cut(s) 209, 306, 403, 461, 1261, 1284, 1348
Kzo9I GATC 8 cut(s) 283, 306, 516, 523, 759, 860, 964, 1363
LmnI GCTCC 4 cut(s) 251, 418, 713, 1461
Lsp1109I GCAGC 2 cut(s) 435, 650
LweI GCATC 5 cut(s) 68, 1308, 1348, 1473, 1494
MaeI CTAG 2 cut(s) 275, 941
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 6 cut(s) 536, 563, 623, 689, 1107, 1439
MalI GATC 8 cut(s) 285, 308, 518, 525, 761, 862, 966, 1365
MboI GATC 8 cut(s) 283, 306, 516, 523, 759, 860, 964, 1363
MboII GAAGA 7 cut(s) 222, 545, 935, 1091, 1154, 1280, 1481
MflI RGATCY 4 cut(s) 516, 523, 964, 1363
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 48, 193, 1004, 1055, 1098, 1273
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 552, 763
MmeI TCCRAC 1 cut(s) 885
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 51, 467, 839, 1073, 1235, 1322
MslI CAYNNNNRTG 2 cut(s) 1125, 1420
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 353, 414, 774, 892
MspR9I CCNGG 3 cut(s) 414, 415, 774
MwoI GCNNNNNNNGC 1 cut(s) 454
NciI CCSGG 3 cut(s) 414, 415, 774
NcoI CCATGG 1 cut(s) 302
NdeII GATC 8 cut(s) 283, 306, 516, 523, 759, 860, 964, 1363
NheI GCTAGC 1 cut(s) 274
NlaIII CATG 7 cut(s) 209, 306, 403, 461, 1261, 1284, 1348
NmuCI GTSAC 2 cut(s) 563, 1439
NspI RCATGY 1 cut(s) 461
PfeI GAWTC 3 cut(s) 369, 908, 1336
PflMI CCANNNNNTGG 1 cut(s) 718
PkrI GCNGC 3 cut(s) 450, 640, 1314
PleI GAGTC 2 cut(s) 552, 763
PpsI GAGTC 2 cut(s) 552, 763
PshBI ATTAAT 1 cut(s) 51
PsiI TTATAA 1 cut(s) 1037
PspFI CCCAGC 1 cut(s) 711
PspPI GGNCC 3 cut(s) 29, 721, 1425
PsrI GAACNNNNNNTAC 2 cut(s) 1074, 1106
PstNI CAGNNNCTG 1 cut(s) 534
PsuI RGATCY 4 cut(s) 516, 523, 964, 1363
RsaI GTAC 2 cut(s) 781, 1092
RsaNI GTAC 2 cut(s) 780, 1091
RseI CAYNNNNRTG 2 cut(s) 1125, 1420
SaqAI TTAA 6 cut(s) 51, 467, 839, 1073, 1235, 1322
SatI GCNGC 3 cut(s) 449, 639, 1313
Sau3AI GATC 8 cut(s) 283, 306, 516, 523, 759, 860, 964, 1363
Sau96I GGNCC 3 cut(s) 29, 721, 1425
SchI GAGTC 2 cut(s) 552, 763
ScrFI CCNGG 3 cut(s) 414, 415, 774
SfaNI GCATC 5 cut(s) 68, 1308, 1348, 1473, 1494
SfcI CTRYAG 2 cut(s) 188, 607
SinI GGWCC 1 cut(s) 721
SmaI CCCGGG 1 cut(s) 415
SmiMI CAYNNNNRTG 2 cut(s) 1125, 1420
SmlI CTYRAG 2 cut(s) 512, 830
SmoI CTYRAG 2 cut(s) 512, 830
Sse9I AATT 6 cut(s) 48, 193, 1004, 1055, 1098, 1273
SsiI CCGC 3 cut(s) 790, 895, 1312
SspMI CTAG 2 cut(s) 275, 941
StyD4I CCNGG 3 cut(s) 412, 413, 772
StyI CCWWGG 2 cut(s) 302, 1147
TaaI ACNGT 8 cut(s) 239, 536, 683, 1030, 1043, 1113, 1403, 1455
TaiI ACGT 1 cut(s) 393
TasI AATT 6 cut(s) 48, 193, 1004, 1055, 1098, 1273
TauI GCSGC 1 cut(s) 1315
TfiI GAWTC 3 cut(s) 369, 908, 1336
Tru1I TTAA 6 cut(s) 51, 467, 839, 1073, 1235, 1322
Tru9I TTAA 6 cut(s) 51, 467, 839, 1073, 1235, 1322
TscAI CASTG 2 cut(s) 723, 1506
TseFI GTSAC 2 cut(s) 563, 1439
TseI GCWGC 2 cut(s) 448, 638
Tsp45I GTSAC 2 cut(s) 563, 1439
TspDTI ATGAA 2 cut(s) 258, 1361
TspGWI ACGGA 1 cut(s) 366
TspMI CCCGGG 1 cut(s) 413
TspRI CASTG 2 cut(s) 723, 1506
Van91I CCANNNNNTGG 1 cut(s) 718
VpaK11BI GGWCC 1 cut(s) 721
VspI ATTAAT 1 cut(s) 51
XapI RAATTY 4 cut(s) 193, 1004, 1055, 1273
XbaI TCTAGA 1 cut(s) 940
XceI RCATGY 1 cut(s) 461
XmaI CCCGGG 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 187
XspI CTAG 2 cut(s) 275, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.