Rroxscaffold_152G00434870

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000152
OG0002272
Physical Location & Seq
Reverse (-)
404198 .. 405818
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_152G00434870.1

Sequence Viewer

Length: 792 bp
ATGGATCGGATATCGCAGTTGCCGGAAGCCCTTATGCTGGAAATCCTCCTCCGGTTGCCTCCGATAGATTCAGTACGTCTGAAAATCACGTCGAGGACATGGAACTCACTCCTGGTTTCTTATTTTTGTCCTCCTATATTAAGTAGTTGGGATGAACATTCTGTCTCTAGTATTGATCAATCCTTGAGAAAACTCCATGAACTGCATCCGAAAAACATATTTCACTGCTTTACCGTGCGTGAATACCTTTCGGACGAAAGTAATGATCCCCATGTCTACAGATGGATAAAGTTGGCCACCAAACACTGTCTGAATCAGCTAAATCTCCACATCTATGCAACAAAAAAATCCACCTCCGGCGGTTTTTTGTTGGATTCTACCAAGCTTGAAGAACTGAATGTTCGCCTAGATAAGAGCGATATTCCTAAAGGTCTTGACTTCTTTTTTACTTTGCAAAAGAGATTTCGAGAATTCGCTGCACAAGAACGCTCGACTGTTTTTATAAACGGTTCAGAAGTCAAATTTTGTCTGAAACTATTAGAAGAAGAACCATCAGTACCAGAAATTATGCAGTTACAGTTGAAACCTAGAGATGATTTTACACGGGAAGACCTCGGCTATTGCTTTTCAACTTTACTTGATAACTTGCTGTGGAGTTGTCATCCAGTGGCCTTATCCATCACAGAAAATGGATGGATTTTAAAGGCCATCTGCAAGAAATTCATGTCTTGGGAAGAGAATTCACCATGTTGTAGAGAGACAACAGCATCAAATGTTGGCGGCACTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

263

Amino Acids

30.43

Weight (kDa)

6.25

Isoelectric Point (pI)

49.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 4 - 38 6e-08 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 503
AccI GTMKAC 1 cut(s) 276
AciI CCGC 2 cut(s) 360, 780
AclWI GGATC 2 cut(s) 12, 260
AcoI YGGCCR 1 cut(s) 294
AcsI RAATTY 4 cut(s) 470, 521, 719, 739
AfaI GTAC 2 cut(s) 75, 558
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 3 cut(s) 389, 583, 630
AjiI CACGTC 1 cut(s) 90
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 2 cut(s) 319, 385
AluI AGCT 2 cut(s) 319, 385
Alw26I GTCTC 2 cut(s) 169, 752
AlwI GGATC 2 cut(s) 12, 260
AoxI GGCC 3 cut(s) 294, 669, 705
ApeKI GCWGC 1 cut(s) 476
ApoI RAATTY 4 cut(s) 470, 521, 719, 739
AsuHPI GGTGA 1 cut(s) 735
BalI TGGCCA 1 cut(s) 296
BbsI GAAGAC 1 cut(s) 615
BbvI GCAGC 1 cut(s) 463
BccI CCATC 5 cut(s) 276, 559, 686, 687, 716
BciT130I CCWGG 1 cut(s) 113
BclI TGATCA 1 cut(s) 175
BcoDI GTCTC 2 cut(s) 169, 752
BfaI CTAG 3 cut(s) 168, 407, 588
BfmI CTRYAG 1 cut(s) 277
BisI GCNGC 2 cut(s) 477, 781
BlsI GCNGC 2 cut(s) 478, 782
Bme1390I CCNGG 1 cut(s) 113
BmgBI CACGTC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 2 cut(s) 214, 776
BpiI GAAGAC 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 205
BsaJI CCNNGG 1 cut(s) 613
BsaWI WCCGGW 1 cut(s) 51
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 665
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 613
BseGI GGATG 4 cut(s) 157, 205, 661, 698
BseLI CCNNNNNNNGG 1 cut(s) 37
BseNI ACTGG 1 cut(s) 665
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 1 cut(s) 463
BsgI GTGCAG 1 cut(s) 462
BshFI GGCC 3 cut(s) 296, 671, 707
BsiSI CCGG 3 cut(s) 23, 52, 357
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 2 cut(s) 169, 752
BsnI GGCC 3 cut(s) 296, 671, 707
Bsp143I GATC 3 cut(s) 4, 175, 265
BspACI CCGC 2 cut(s) 360, 780
BspANI GGCC 3 cut(s) 296, 671, 707
BspPI GGATC 2 cut(s) 12, 260
BsrI ACTGG 1 cut(s) 665
BssECI CCNNGG 1 cut(s) 613
BssMI GATC 3 cut(s) 4, 175, 265
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 5 cut(s) 235, 308, 496, 509, 579
Bst6I CTCTTC 1 cut(s) 729
BstF5I GGATG 4 cut(s) 157, 205, 661, 698
BstKTI GATC 3 cut(s) 7, 178, 268
BstMAI GTCTC 2 cut(s) 169, 752
BstMBI GATC 3 cut(s) 4, 175, 265
BstNI CCWGG 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 111
BstSFI CTRYAG 1 cut(s) 277
BstV1I GCAGC 1 cut(s) 463
BstV2I GAAGAC 1 cut(s) 615
BsuRI GGCC 3 cut(s) 296, 671, 707
BtrI CACGTC 1 cut(s) 90
BtsCI GGATG 4 cut(s) 157, 205, 661, 698
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 3 cut(s) 223, 304, 672
Csp6I GTAC 2 cut(s) 74, 557
CviAII CATG 5 cut(s) 99, 197, 272, 724, 747
CviJI RGCY 7 cut(s) 29, 296, 319, 385, 618, 671, 707
CviKI_1 RGCY 7 cut(s) 29, 296, 319, 385, 618, 671, 707
CviQI GTAC 2 cut(s) 74, 557
DpnI GATC 3 cut(s) 6, 177, 267
DpnII GATC 3 cut(s) 4, 175, 265
DraI TTTAAA 1 cut(s) 702
EaeI YGGCCR 1 cut(s) 294
Eam1104I CTCTTC 1 cut(s) 729
EarI CTCTTC 1 cut(s) 729
Eco32I GATATC 1 cut(s) 12
EcoRI GAATTC 2 cut(s) 470, 739
EcoRII CCWGG 1 cut(s) 111
EcoRV GATATC 1 cut(s) 12
FaeI CATG 5 cut(s) 102, 200, 275, 727, 750
FatI CATG 5 cut(s) 98, 196, 271, 723, 746
FbaI TGATCA 1 cut(s) 175
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 477, 781
FokI GGATG 4 cut(s) 164, 192, 648, 705
Fsp4HI GCNGC 2 cut(s) 477, 781
FspBI CTAG 3 cut(s) 168, 407, 588
GluI GCNGC 2 cut(s) 477, 781
HaeIII GGCC 3 cut(s) 296, 671, 707
HapII CCGG 3 cut(s) 23, 52, 357
Hin1II CATG 5 cut(s) 102, 200, 275, 727, 750
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 3 cut(s) 68, 313, 374
HpaII CCGG 3 cut(s) 23, 52, 357
HphI GGTGA 1 cut(s) 735
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 8 cut(s) 9, 63, 81, 210, 253, 312, 514, 531
Hpy188III TCNNGA 2 cut(s) 434, 467
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 1 cut(s) 94
HpyCH4III ACNGT 5 cut(s) 235, 308, 496, 509, 579
HpyCH4IV ACGT 2 cut(s) 76, 89
HpyCH4V TGCA 6 cut(s) 205, 338, 454, 479, 571, 714
HpySE526I ACGT 2 cut(s) 76, 89
Hsp92II CATG 5 cut(s) 102, 200, 275, 727, 750
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 3 cut(s) 4, 175, 265
LpnPI CCDG 8 cut(s) 23, 36, 65, 98, 125, 370, 573, 678
Lsp1109I GCAGC 1 cut(s) 463
LweI GCATC 2 cut(s) 214, 776
MaeI CTAG 3 cut(s) 168, 407, 588
MaeII ACGT 2 cut(s) 76, 89
MaeIII GTNAC 1 cut(s) 573
MalI GATC 3 cut(s) 6, 177, 267
MboI GATC 3 cut(s) 4, 175, 265
MboII GAAGA 5 cut(s) 401, 554, 557, 620, 746
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 5 cut(s) 470, 521, 564, 719, 739
MluNI TGGCCA 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 7 cut(s) 56, 59, 69, 87, 141, 364, 623
Mox20I TGGCCA 1 cut(s) 296
MscI TGGCCA 1 cut(s) 296
MseI TTAA 3 cut(s) 140, 701, 790
MslI CAYNNNNRTG 1 cut(s) 333
Msp20I TGGCCA 1 cut(s) 296
MspI CCGG 3 cut(s) 23, 52, 357
MspR9I CCNGG 1 cut(s) 113
MvaI CCWGG 1 cut(s) 113
NdeII GATC 3 cut(s) 4, 175, 265
NlaIII CATG 5 cut(s) 102, 200, 275, 727, 750
NmeAIII GCCGAG 1 cut(s) 594
PfeI GAWTC 3 cut(s) 68, 313, 374
PkrI GCNGC 2 cut(s) 478, 782
PsiI TTATAA 1 cut(s) 503
Psp6I CCWGG 1 cut(s) 111
PspGI CCWGG 1 cut(s) 111
PsrI GAACNNNNNNTAC 2 cut(s) 540, 572
RsaI GTAC 2 cut(s) 75, 558
RsaNI GTAC 2 cut(s) 74, 557
RseI CAYNNNNRTG 1 cut(s) 333
SaqAI TTAA 3 cut(s) 140, 701, 790
SatI GCNGC 2 cut(s) 477, 781
Sau3AI GATC 3 cut(s) 4, 175, 265
ScrFI CCNGG 1 cut(s) 113
SetI ASST 9 cut(s) 79, 92, 249, 321, 356, 387, 433, 589, 615
SfaNI GCATC 2 cut(s) 214, 776
SfcI CTRYAG 1 cut(s) 277
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 184
SmoI CTYRAG 1 cut(s) 184
Sse9I AATT 5 cut(s) 470, 521, 564, 719, 739
SsiI CCGC 2 cut(s) 360, 780
SspMI CTAG 3 cut(s) 168, 407, 588
StyD4I CCNGG 1 cut(s) 111
TaaI ACNGT 5 cut(s) 235, 308, 496, 509, 579
TaiI ACGT 2 cut(s) 79, 92
TaqI TCGA 3 cut(s) 92, 466, 491
TasI AATT 5 cut(s) 470, 521, 564, 719, 739
TauI GCSGC 1 cut(s) 783
TfiI GAWTC 3 cut(s) 68, 313, 374
Tru1I TTAA 3 cut(s) 140, 701, 790
Tru9I TTAA 3 cut(s) 140, 701, 790
TscAI CASTG 3 cut(s) 230, 311, 672
TseI GCWGC 1 cut(s) 476
TspDTI ATGAA 3 cut(s) 168, 213, 712
TspRI CASTG 3 cut(s) 230, 311, 672
XapI RAATTY 4 cut(s) 470, 521, 719, 739
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 3 cut(s) 168, 407, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.