Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0002272

Physical Location & Seq

Genomic Coordinates Forward (+)

65058725 .. 65060517

Gene Structure 1793 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG439900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGATCAAATATCTGAGTTGGTGGAACCCCTTATGGGGACACAGCCAGTCGCTGCTTTTAATTTTAACAACTCGGCTTATTTCGGAGGCACAAGAAGGTCCCGAAAGAAGTTTGTGACTTTTGTTGATGATTCCTTGGAAAGAAACCTCCCTGAGCATGAAGACCAGAAAAAGTTCGTTCAGTTCTCGTTGGCTATTGCAACCCTTGACAGCAAGCTGAAAGACTCTGATGTTGTCGACCGCTGGATAAAGATGGTTACCAAACACTATGTGACAGAGTTAAAACTCCGGATCAAAAACGCACCAAAGATTAGGTACCAATTTCCCCCGGCTGCTTTTGATGTTGGGCAATTAGTTGTACTGGAGCTAATGCATTGTACTTTGGAGAAAGCCTTTATTCAAGAGGATACTATGTTTCTTCCAATTCTTCGGTTAAAAGAGCTCAGATTCAGTCATGTCAATTTCAATGGGGTAGTCGTCGGGGAGCTGCTGTCTAGATGCCCTTTGCTTGAGACTTTGGCATTACGCTCCTGTGGAAACATAAAACATCTCGAACTTTGTGATTTTCCAAATCTCAAGAATGTTGAACTTTGGAATGATTCAGATGATGATGATGATATTTTTACCTACAAGATTGACTCACCCGATCTCAAATCTCTTCTTTATTTGGAGTATGGAAAGTCAATGGACATCGAAGCAGTCACTTGCAAAAATTTAAAGACGCTGCATATAGTTACTCATGGCTCTACCATTACGGACCAATATATCAAGGAAGTCAATTCCAAATTTCCTTTTCTTGAAACCTTGATACTGAATGCCGGATCTTTTGGATCAAAAGATGCTGATGAGATTAGTATTTCGGGTCATAACCTCAATTCACTGCTAGTTTGTGGACATATTTGCAAACATACGATCATCAATATAGATGCACCAAATCTTCTTAGTTACCAACGCGAGGGTGACGAAATTCCTCCCTCGTTTTCGTTGAATTCTGTGAAGCTAGAATGGGCTAACTTTATTGTACCTGGAGCTCATCTCAACAGTGCATGGTTTATTAAATTGCGAGAATATCTGGGAAAGTTTAAGGGTTGTGATCATCTTCGCTTCAGAATATGTCATAAGCAGCCTTGCAAGACAGTCAAATTTTCTTTTGACGAATTACAAGAAGAAAAACTTCCTCCCCTGCCAAAGGTCAGGCAATTGGTTCTGAAAAATGTTCCACCATCGAGCGACTATACAACTCTTATCGATGGCTTACTTTGGAGTTGCCATCCACTCACCATATCAGTACAAGCACGCGGTGCACCTAACCGTAAATTTATAAAGGTCTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box

Protein Analysis

444

Amino Acids

50.63

Weight (kDa)

6.91

Isoelectric Point (pI)

41.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_At1g61320_AtMIF1	PF23622	64 - 273	2e-14	At1g61320/AtMIF1, LRR domain
LRR_At5g56370	PF24758	79 - 248	7.1e-16	FBD-associated F-box protein At5g56370, LRR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002272)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g26980 FvH4_6g32452 FvH4_6g32461 FvH4_6g32741
prunus_persica	Prupe.3G054600_v2.0.a1 Prupe.3G054700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0395301
rosa_laevigata	RLG00000020089 RLG00000020183
rosa_multiflora	Rmu_sc0001483.1_g000030 Rmu_sc0002727.1_g000001 Rmu_sc0005789.1_g000002 Rmu_sc0010454.1_g000003 Rmu_sc0010817.1_g000032 Rmu_sc0035624.1_g000001
rosa_roxburghii	Rroxscaffold_152G00434870 Rroxscaffold_2G00100290 Rroxscaffold_2G00103790 Rroxscaffold_2G00104560
rosa_rugosa	Rorug03G0358200
rosa_samantha	Rh2AG420800 Rh2AG439900 Rh2AG449200 Rh2BG425500 Rh2BG430600 Rh2BG449900 Rh2BG461600 Rh2CG407800 Rh2CG426900 Rh2CG436100 Rh2DG440500 Rh2DG459800 Rh2DG470800 Rh4AG058300 Rh4BG057100 Rh4CG062900 Rh4DG053800 Rh6CG063800 Rh6CG064600 Rh6DG060900 Rh6DG061600
rosa_wichuraiana	Rw2G035990 Rw4G004690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1322
Acc65I	GGTACC	1 cut(s)	315
AccB1I	GGYRCC	1 cut(s)	315
AccI	GTMKAC	1 cut(s)	237
AccII	CGCG	2 cut(s)	954, 1299
AccIII	TCCGGA	1 cut(s)	288
AciI	CCGC	2 cut(s)	241, 1299
AclWI	GGATC	4 cut(s)	12, 299, 829, 838
AcsI	RAATTY	6 cut(s)	712, 785, 966, 988, 1142, 1316
AcuI	CTGAAG	1 cut(s)	1090
AdeI	CACNNNGTG	2 cut(s)	271, 1301
AfaI	GTAC	5 cut(s)	317, 360, 379, 1023, 1290
AfiI	CCNNNNNNNGG	4 cut(s)	35, 36, 955, 1189
AgsI	TTSAA	5 cut(s)	401, 466, 587, 800, 988
AjnI	CCWGG	1 cut(s)	1024
AluBI	AGCT	6 cut(s)	217, 367, 442, 487, 1000, 1031
AluI	AGCT	6 cut(s)	217, 367, 442, 487, 1000, 1031
Alw21I	GWGCWC	3 cut(s)	444, 1033, 1306
Alw26I	GTCTC	1 cut(s)	506
Alw44I	GTGCAC	1 cut(s)	1302
AlwI	GGATC	4 cut(s)	12, 299, 829, 838
AlwNI	CAGNNNCTG	1 cut(s)	53
Aor13HI	TCCGGA	1 cut(s)	288
ApaLI	GTGCAC	1 cut(s)	1302
ApeKI	GCWGC	5 cut(s)	53, 332, 487, 724, 1123
ApoI	RAATTY	6 cut(s)	712, 785, 966, 988, 1142, 1316
Asp700I	GAANNNNTTC	2 cut(s)	173, 1173
Asp718I	GGTACC	1 cut(s)	315
AspS9I	GGNCC	2 cut(s)	99, 757
AsuC2I	CCSGG	1 cut(s)	329
AsuHPI	GGTGA	3 cut(s)	633, 971, 1270
AvaII	GGWCC	2 cut(s)	99, 757
BaeGI	GKGCMC	1 cut(s)	1306
BanI	GGYRCC	1 cut(s)	315
BanII	GRGCYC	2 cut(s)	444, 1033
BbsI	GAAGAC	1 cut(s)	168
Bbv12I	GWGCWC	3 cut(s)	444, 1033, 1306
BbvI	GCAGC	5 cut(s)	40, 319, 474, 711, 1135
BccI	CCATC	4 cut(s)	247, 1231, 1244, 1278
BciT130I	CCWGG	1 cut(s)	1026
BciVI	GTATCC	1 cut(s)	400
BclI	TGATCA	1 cut(s)	1093
BcnI	CCSGG	1 cut(s)	329
BcoDI	GTCTC	1 cut(s)	506
BfaI	CTAG	3 cut(s)	495, 884, 1001
BfuI	GTATCC	1 cut(s)	400
BisI	GCNGC	5 cut(s)	54, 333, 488, 725, 1124
BlsI	GCNGC	5 cut(s)	55, 334, 489, 726, 1125
Bme1390I	CCNGG	2 cut(s)	329, 1026
Bme18I	GGWCC	2 cut(s)	99, 757
BmgT120I	GGNCC	2 cut(s)	99, 757
BmiI	GGNNCC	3 cut(s)	27, 101, 317
BmrFI	CCNGG	2 cut(s)	329, 1026
BmsI	GCATC	3 cut(s)	488, 829, 916
BpiI	GAAGAC	1 cut(s)	168
BplI	GAGNNNNNCTC	2 cut(s)	1020, 1052
BpmI	CTGGAG	2 cut(s)	383, 1047
Bpu10I	CCTNAGC	1 cut(s)	153
BpuEI	CTTGAG	2 cut(s)	530, 560
BpuMI	CCSGG	1 cut(s)	329
Bsa29I	ATCGAT	1 cut(s)	1248
BsaJI	CCNNGG	2 cut(s)	135, 327
BsaWI	WCCGGW	1 cut(s)	288
BsaXI	ACNNNNNCTCC	2 cut(s)	476, 506
Bsc4I	CCNNNNNNNGG	4 cut(s)	35, 36, 955, 1189
Bse1I	ACTGG	2 cut(s)	47, 366
BseAI	TCCGGA	1 cut(s)	288
BseBI	CCWGG	1 cut(s)	1026
BseCI	ATCGAT	1 cut(s)	1248
BseDI	CCNNGG	2 cut(s)	135, 327
BseGI	GGATG	1 cut(s)	1270
BseLI	CCNNNNNNNGG	4 cut(s)	35, 36, 955, 1189
BseMII	CTCAG	3 cut(s)	6, 144, 457
BseNI	ACTGG	2 cut(s)	47, 366
BseSI	GKGCMC	1 cut(s)	1306
BseXI	GCAGC	5 cut(s)	40, 319, 474, 711, 1135
Bsh1236I	CGCG	2 cut(s)	954, 1299
Bsh1285I	CGRYCG	1 cut(s)	241
BshNI	GGYRCC	1 cut(s)	315
BshVI	ATCGAT	1 cut(s)	1248
BsiEI	CGRYCG	1 cut(s)	241
BsiHKAI	GWGCWC	3 cut(s)	444, 1033, 1306
BsiSI	CCGG	3 cut(s)	289, 329, 819
BslFI	GGGAC	2 cut(s)	52, 85
BslI	CCNNNNNNNGG	4 cut(s)	35, 36, 955, 1189
BsmAI	GTCTC	1 cut(s)	506
BsmFI	GGGAC	2 cut(s)	52, 85
BsmI	GAATGC	1 cut(s)	820
Bsp1286I	GDGCHC	3 cut(s)	444, 1033, 1306
Bsp13I	TCCGGA	1 cut(s)	288
Bsp143I	GATC	7 cut(s)	4, 291, 646, 821, 830, 912, 1093
BspACI	CCGC	2 cut(s)	241, 1299
BspCNI	CTCAG	3 cut(s)	7, 145, 456
BspDI	ATCGAT	1 cut(s)	1248
BspEI	TCCGGA	1 cut(s)	288
BspFNI	CGCG	2 cut(s)	954, 1299
BspLI	GGNNCC	3 cut(s)	27, 101, 317
BspPI	GGATC	4 cut(s)	12, 299, 829, 838
BspT107I	GGYRCC	1 cut(s)	315
BsrI	ACTGG	2 cut(s)	47, 366
BssECI	CCNNGG	2 cut(s)	135, 327
BssMI	GATC	7 cut(s)	4, 291, 646, 821, 830, 912, 1093
BssT1I	CCWWGG	1 cut(s)	135
Bst2UI	CCWGG	1 cut(s)	1026
Bst4CI	ACNGT	3 cut(s)	1043, 1138, 1313
Bst6I	CTCTTC	1 cut(s)	663
BstAPI	GCANNNNNTGC	1 cut(s)	1301
BstC8I	GCNNGC	2 cut(s)	215, 1297
BstDEI	CTNAG	4 cut(s)	15, 153, 443, 941
BstEII	GGTNACC	1 cut(s)	256
BstENI	CCTNNNNNAGG	1 cut(s)	1187
BstF5I	GGATG	1 cut(s)	1270
BstFNI	CGCG	2 cut(s)	954, 1299
BstKTI	GATC	7 cut(s)	7, 294, 649, 824, 833, 915, 1096
BstMAI	GTCTC	1 cut(s)	506
BstMBI	GATC	7 cut(s)	4, 291, 646, 821, 830, 912, 1093
BstMCI	CGRYCG	1 cut(s)	241
BstMWI	GCNNNNNNNGC	1 cut(s)	1301
BstNI	CCWGG	1 cut(s)	1026
BstPI	GGTNACC	1 cut(s)	256
BstSCI	CCNGG	2 cut(s)	327, 1024
BstSLI	GKGCMC	1 cut(s)	1306
BstUI	CGCG	2 cut(s)	954, 1299
BstV1I	GCAGC	5 cut(s)	40, 319, 474, 711, 1135
BstV2I	GAAGAC	1 cut(s)	168
BstX2I	RGATCY	1 cut(s)	821
BstYI	RGATCY	1 cut(s)	821
Bsu15I	ATCGAT	1 cut(s)	1248
BsuI	GTATCC	1 cut(s)	400
BsuTUI	ATCGAT	1 cut(s)	1248
BtsCI	GGATG	1 cut(s)	1270
BtsI	GCAGTG	1 cut(s)	878
BtsIMutI	CAGTG	2 cut(s)	878, 1048
Cac8I	GCNNGC	2 cut(s)	215, 1297
CaiI	CAGNNNCTG	1 cut(s)	53
Cfr13I	GGNCC	2 cut(s)	99, 757
ClaI	ATCGAT	1 cut(s)	1248
CseI	GACGC	1 cut(s)	730
Csp6I	GTAC	5 cut(s)	316, 359, 378, 1022, 1289
CviAII	CATG	4 cut(s)	158, 455, 740, 1047
CviQI	GTAC	5 cut(s)	316, 359, 378, 1022, 1289
DdeI	CTNAG	4 cut(s)	15, 153, 443, 941
DpnI	GATC	7 cut(s)	6, 293, 648, 823, 832, 914, 1095
DpnII	GATC	7 cut(s)	4, 291, 646, 821, 830, 912, 1093
DraI	TTTAAA	1 cut(s)	717
DraIII	CACNNNGTG	2 cut(s)	271, 1301
Eam1104I	CTCTTC	1 cut(s)	663
EarI	CTCTTC	1 cut(s)	663
Ecl136II	GAGCTC	2 cut(s)	442, 1031
Eco130I	CCWWGG	1 cut(s)	135
Eco24I	GRGCYC	2 cut(s)	444, 1033
Eco47I	GGWCC	2 cut(s)	99, 757
Eco53kI	GAGCTC	2 cut(s)	442, 1031
Eco57I	CTGAAG	1 cut(s)	1090
Eco91I	GGTNACC	1 cut(s)	256
EcoICRI	GAGCTC	2 cut(s)	442, 1031
EcoNI	CCTNNNNNAGG	1 cut(s)	1187
EcoO109I	RGGNCCY	1 cut(s)	99
EcoO65I	GGTNACC	1 cut(s)	256
EcoRI	GAATTC	1 cut(s)	988
EcoRII	CCWGG	1 cut(s)	1024
EcoT14I	CCWWGG	1 cut(s)	135
EcoT22I	ATGCAT	1 cut(s)	375
EcoT38I	GRGCYC	2 cut(s)	444, 1033
ErhI	CCWWGG	1 cut(s)	135
FaeI	CATG	4 cut(s)	161, 458, 743, 1050
FalI	AAGNNNNNCTT	2 cut(s)	1158, 1190
FaqI	GGGAC	2 cut(s)	52, 85
FatI	CATG	4 cut(s)	157, 454, 739, 1046
FbaI	TGATCA	1 cut(s)	1093
FblI	GTMKAC	1 cut(s)	237
Fnu4HI	GCNGC	5 cut(s)	54, 333, 488, 725, 1124
FokI	GGATG	1 cut(s)	1257
FriOI	GRGCYC	2 cut(s)	444, 1033
Fsp4HI	GCNGC	5 cut(s)	54, 333, 488, 725, 1124
FspBI	CTAG	3 cut(s)	495, 884, 1001
GluI	GCNGC	5 cut(s)	54, 333, 488, 725, 1124
GsuI	CTGGAG	2 cut(s)	383, 1047
HapII	CCGG	3 cut(s)	289, 329, 819
HgaI	GACGC	1 cut(s)	730
Hin1II	CATG	4 cut(s)	161, 458, 743, 1050
HincII	GTYRAC	1 cut(s)	238
HindII	GTYRAC	1 cut(s)	238
HinfI	GANTC	5 cut(s)	131, 224, 447, 599, 638
HpaII	CCGG	3 cut(s)	289, 329, 819
HphI	GGTGA	3 cut(s)	633, 971, 1270
Hpy166II	GTNNAC	3 cut(s)	238, 893, 1304
Hpy188I	TCNGA	7 cut(s)	16, 86, 229, 446, 604, 1109, 1209
Hpy188III	TCNNGA	7 cut(s)	102, 289, 401, 495, 551, 577, 797
Hpy8I	GTNNAC	3 cut(s)	238, 893, 1304
Hpy99I	CGWCG	1 cut(s)	482
HpyAV	CCTTC	1 cut(s)	90
HpyCH4III	ACNGT	3 cut(s)	1043, 1138, 1313
HpyCH4V	TGCA	9 cut(s)	200, 373, 708, 727, 903, 929, 1046, 1131, 1304
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1301
HpyF3I	CTNAG	4 cut(s)	15, 153, 443, 941
Hsp92II	CATG	4 cut(s)	161, 458, 743, 1050
Kpn2I	TCCGGA	1 cut(s)	288
KpnI	GGTACC	1 cut(s)	319
Ksp22I	TGATCA	1 cut(s)	1093
Kzo9I	GATC	7 cut(s)	4, 291, 646, 821, 830, 912, 1093
LmnI	GCTCC	4 cut(s)	364, 484, 533, 1028
Lsp1109I	GCAGC	5 cut(s)	40, 319, 474, 711, 1135
LweI	GCATC	3 cut(s)	488, 829, 916
MaeI	CTAG	3 cut(s)	495, 884, 1001
MaeIII	GTNAC	7 cut(s)	115, 256, 271, 700, 733, 944, 959
MalI	GATC	7 cut(s)	6, 293, 648, 823, 832, 914, 1095
MboI	GATC	7 cut(s)	4, 291, 646, 821, 830, 912, 1093
MboII	GAAGA	7 cut(s)	173, 410, 419, 650, 929, 1091, 1178
MfeI	CAATTG	1 cut(s)	1199
MflI	RGATCY	1 cut(s)	821
MhlI	GDGCHC	3 cut(s)	444, 1033, 1306
MlyI	GAGTC	2 cut(s)	218, 632
MnlI	CCTC	8 cut(s)	80, 158, 397, 881, 949, 981, 985, 1188
Mph1103I	ATGCAT	1 cut(s)	375
MroI	TCCGGA	1 cut(s)	288
MroXI	GAANNNNTTC	2 cut(s)	173, 1173
MseI	TTAA	7 cut(s)	60, 66, 281, 434, 716, 1056, 1083
MspA1I	CMGCKG	1 cut(s)	243
MspI	CCGG	3 cut(s)	289, 329, 819
MspR9I	CCNGG	2 cut(s)	329, 1026
MunI	CAATTG	1 cut(s)	1199
Mva1269I	GAATGC	1 cut(s)	820
MvaI	CCWGG	1 cut(s)	1026
MvnI	CGCG	2 cut(s)	954, 1299
MwoI	GCNNNNNNNGC	1 cut(s)	1301
NciI	CCSGG	1 cut(s)	329
NdeII	GATC	7 cut(s)	4, 291, 646, 821, 830, 912, 1093
NlaIII	CATG	4 cut(s)	161, 458, 743, 1050
NlaIV	GGNNCC	3 cut(s)	27, 101, 317
NmeAIII	GCCGAG	1 cut(s)	53
NmuCI	GTSAC	4 cut(s)	115, 271, 700, 959
NsiI	ATGCAT	1 cut(s)	375
PctI	GAATGC	1 cut(s)	820
PdmI	GAANNNNTTC	2 cut(s)	173, 1173
PfeI	GAWTC	3 cut(s)	131, 447, 599
PkrI	GCNGC	5 cut(s)	55, 334, 489, 726, 1125
PleI	GAGTC	2 cut(s)	218, 632
PpsI	GAGTC	2 cut(s)	218, 632
PpuMI	RGGWCCY	1 cut(s)	99
PsiI	TTATAA	1 cut(s)	1322
Psp124BI	GAGCTC	2 cut(s)	444, 1033
Psp5II	RGGWCCY	1 cut(s)	99
Psp6I	CCWGG	1 cut(s)	1024
PspEI	GGTNACC	1 cut(s)	256
PspGI	CCWGG	1 cut(s)	1024
PspN4I	GGNNCC	3 cut(s)	27, 101, 317
PspPI	GGNCC	2 cut(s)	99, 757
PspPPI	RGGWCCY	1 cut(s)	99
PstNI	CAGNNNCTG	1 cut(s)	53
PsuI	RGATCY	1 cut(s)	821
RsaI	GTAC	5 cut(s)	317, 360, 379, 1023, 1290
RsaNI	GTAC	5 cut(s)	316, 359, 378, 1022, 1289
SacI	GAGCTC	2 cut(s)	444, 1033
SalI	GTCGAC	1 cut(s)	236
SaqAI	TTAA	7 cut(s)	60, 66, 281, 434, 716, 1056, 1083
SatI	GCNGC	5 cut(s)	54, 333, 488, 725, 1124
Sau3AI	GATC	7 cut(s)	4, 291, 646, 821, 830, 912, 1093
Sau96I	GGNCC	2 cut(s)	99, 757
SchI	GAGTC	2 cut(s)	218, 632
ScrFI	CCNGG	2 cut(s)	329, 1026
SduI	GDGCHC	3 cut(s)	444, 1033, 1306
SfaNI	GCATC	3 cut(s)	488, 829, 916
SinI	GGWCC	2 cut(s)	99, 757
SmlI	CTYRAG	2 cut(s)	509, 575
SmoI	CTYRAG	2 cut(s)	509, 575
SsiI	CCGC	2 cut(s)	241, 1299
SspMI	CTAG	3 cut(s)	495, 884, 1001
SstI	GAGCTC	2 cut(s)	444, 1033
StyD4I	CCNGG	2 cut(s)	327, 1024
StyI	CCWWGG	1 cut(s)	135
TaaI	ACNGT	3 cut(s)	1043, 1138, 1313
TaqI	TCGA	5 cut(s)	237, 552, 693, 1226, 1248
TatI	WGTACW	3 cut(s)	358, 377, 1288
TfiI	GAWTC	3 cut(s)	131, 447, 599
Tru1I	TTAA	7 cut(s)	60, 66, 281, 434, 716, 1056, 1083
Tru9I	TTAA	7 cut(s)	60, 66, 281, 434, 716, 1056, 1083
TscAI	CASTG	2 cut(s)	885, 1048
TseFI	GTSAC	4 cut(s)	115, 271, 700, 959
TseI	GCWGC	5 cut(s)	53, 332, 487, 724, 1123
Tsp45I	GTSAC	4 cut(s)	115, 271, 700, 959
TspDTI	ATGAA	1 cut(s)	174
TspGWI	ACGGA	1 cut(s)	770
TspRI	CASTG	2 cut(s)	885, 1048
VneI	GTGCAC	1 cut(s)	1302
VpaK11BI	GGWCC	2 cut(s)	99, 757
XagI	CCTNNNNNAGG	1 cut(s)	1187
XapI	RAATTY	6 cut(s)	712, 785, 966, 988, 1142, 1316
XbaI	TCTAGA	1 cut(s)	494
XmiI	GTMKAC	1 cut(s)	237
XmnI	GAANNNNTTC	2 cut(s)	173, 1173
XspI	CTAG	3 cut(s)	495, 884, 1001
Zsp2I	ATGCAT	1 cut(s)	375

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.