RLG00000020721

CYSTM1 family protein A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005236
Physical Location & Seq
Reverse (-)
67952337 .. 67954608
2272 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020721

Sequence Viewer

Length: 390 bp
ATGGATCGGAGCCTCGACGACGTCACGGAAAGCAGTGATGATGACGAAGCGGAAGGATTCTGGATCGGGTTGACGTGGAGATCTACATGGTGCTCTACATCGCCTCGCTGCGATGCTGTCGTCGATGCGTTCGAGTTGCCGCCGGAGACAATCGCTGCCGGGGATGAGGTGGCACCGAGTTTGGCGTCGGCGACAGCGTACACCGCTCCGGCTTCGCAACGGGGACCTTACGTCCAGGCTCCGCCTCCTGCCGGCTATCCCACCATGAGGGACGACGCCGACTTGCCCCAGAGTCATGGTCGTGTGGAAACCAAGTCCAAGGGTGATGGCTTCTGGAAGGGCTGCTGTGCTGCCTTGTGTTGCTGTTGTATGCTCGATGCTTGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

130

Amino Acids

13.79

Weight (kDa)

4.15

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 77 - 129 5.2e-11 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 172
AccBSI CCGCTC 1 cut(s) 206
AciI CCGC 4 cut(s) 50, 140, 204, 242
AclWI GGATC 2 cut(s) 12, 71
AcyI GRCGYC 3 cut(s) 21, 185, 276
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 251, 267
AhdI GACNNNNNGTC 1 cut(s) 230
AjiI CACGTC 1 cut(s) 75
AjnI CCWGG 1 cut(s) 234
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 2 cut(s) 12, 71
ApeKI GCWGC 4 cut(s) 108, 155, 342, 350
AspS9I GGNCC 1 cut(s) 224
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 335
AvaII GGWCC 1 cut(s) 224
BanI GGYRCC 1 cut(s) 172
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 4 cut(s) 95, 142, 329, 337
BccI CCATC 1 cut(s) 320
BciT130I CCWGG 1 cut(s) 236
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 140
BglII AGATCT 1 cut(s) 80
BisI GCNGC 5 cut(s) 109, 140, 156, 343, 351
BlsI GCNGC 5 cut(s) 110, 141, 157, 344, 352
Bme1390I CCNGG 2 cut(s) 160, 236
Bme18I GGWCC 1 cut(s) 224
BmeRI GACNNNNNGTC 1 cut(s) 230
BmgBI CACGTC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 4 cut(s) 11, 174, 225, 240
BmrFI CCNGG 2 cut(s) 160, 236
BmsI GCATC 3 cut(s) 103, 115, 367
BpuMI CCSGG 1 cut(s) 160
BsaHI GRCGYC 3 cut(s) 21, 185, 276
BsaJI CCNNGG 2 cut(s) 159, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 267
Bse118I RCCGGY 1 cut(s) 251
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 2 cut(s) 159, 318
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 2 cut(s) 251, 267
BseXI GCAGC 4 cut(s) 95, 142, 329, 337
BshNI GGYRCC 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 4 cut(s) 143, 159, 209, 252
BslFI GGGAC 2 cut(s) 237, 284
BslI CCNNNNNNNGG 2 cut(s) 251, 267
BsmAI GTCTC 1 cut(s) 140
BsmFI GGGAC 2 cut(s) 237, 284
Bsp1286I GDGCHC 1 cut(s) 95
Bsp143I GATC 3 cut(s) 4, 63, 80
BspACI CCGC 4 cut(s) 50, 140, 204, 242
BspLI GGNNCC 4 cut(s) 11, 174, 225, 240
BspPI GGATC 2 cut(s) 12, 71
BspT107I GGYRCC 1 cut(s) 172
BsrBI CCGCTC 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 251
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 2 cut(s) 159, 318
BssMI GATC 3 cut(s) 4, 63, 80
BssNI GRCGYC 3 cut(s) 21, 185, 276
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 236
BstACI GRCGYC 3 cut(s) 21, 185, 276
BstC8I GCNNGC 2 cut(s) 253, 382
BstF5I GGATG 1 cut(s) 169
BstKTI GATC 3 cut(s) 7, 66, 83
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 3 cut(s) 4, 63, 80
BstMWI GCNNNNNNNGC 1 cut(s) 203
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 158, 234
BstV1I GCAGC 4 cut(s) 95, 142, 329, 337
BstX2I RGATCY 1 cut(s) 80
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 1 cut(s) 80
BtgZI GCGATG 2 cut(s) 84, 126
BtrI CACGTC 1 cut(s) 75
BtsCI GGATG 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 2 cut(s) 253, 382
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 224
CseI GACGC 2 cut(s) 174, 284
Csp6I GTAC 1 cut(s) 199
CviAII CATG 3 cut(s) 87, 265, 296
CviJI RGCY 6 cut(s) 12, 212, 239, 255, 330, 342
CviKI_1 RGCY 6 cut(s) 12, 212, 239, 255, 330, 342
CviQI GTAC 1 cut(s) 199
DpnI GATC 3 cut(s) 6, 65, 82
DpnII GATC 3 cut(s) 4, 63, 80
DriI GACNNNNNGTC 1 cut(s) 230
Eam1105I GACNNNNNGTC 1 cut(s) 230
EciI GGCGGA 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 3 cut(s) 90, 268, 299
FaiI YATR 4 cut(s) 88, 266, 297, 371
FaqI GGGAC 2 cut(s) 237, 284
FatI CATG 3 cut(s) 86, 264, 295
Fnu4HI GCNGC 5 cut(s) 109, 140, 156, 343, 351
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 5 cut(s) 109, 140, 156, 343, 351
GluI GCNGC 5 cut(s) 109, 140, 156, 343, 351
HapII CCGG 4 cut(s) 143, 159, 209, 252
HgaI GACGC 2 cut(s) 174, 284
Hin1I GRCGYC 3 cut(s) 21, 185, 276
Hin1II CATG 3 cut(s) 90, 268, 299
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HinfI GANTC 2 cut(s) 57, 292
HpaII CCGG 4 cut(s) 143, 159, 209, 252
HphI GGTGA 1 cut(s) 335
Hpy166II GTNNAC 2 cut(s) 72, 201
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 2 cut(s) 61, 334
Hpy8I GTNNAC 2 cut(s) 72, 201
Hpy99I CGWCG 5 cut(s) 20, 23, 125, 190, 278
HpyAV CCTTC 2 cut(s) 47, 331
HpyCH4IV ACGT 3 cut(s) 21, 74, 231
HpyF10VI GCNNNNNNNGC 1 cut(s) 203
HpySE526I ACGT 3 cut(s) 21, 74, 231
Hsp92I GRCGYC 3 cut(s) 21, 185, 276
Hsp92II CATG 3 cut(s) 90, 268, 299
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Kzo9I GATC 3 cut(s) 4, 63, 80
LmnI GCTCC 3 cut(s) 9, 211, 244
Lsp1109I GCAGC 4 cut(s) 95, 142, 329, 337
LweI GCATC 3 cut(s) 103, 115, 367
MaeII ACGT 3 cut(s) 21, 74, 231
MaeIII GTNAC 1 cut(s) 22
MalI GATC 3 cut(s) 6, 65, 82
MbiI CCGCTC 1 cut(s) 206
MboI GATC 3 cut(s) 4, 63, 80
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 1 cut(s) 95
MlyI GAGTC 1 cut(s) 301
MnlI CCTC 5 cut(s) 23, 114, 160, 255, 261
MroNI GCCGGC 1 cut(s) 251
MslI CAYNNNNRTG 1 cut(s) 300
MspI CCGG 4 cut(s) 143, 159, 209, 252
MspR9I CCNGG 2 cut(s) 160, 236
MvaI CCWGG 1 cut(s) 236
MwoI GCNNNNNNNGC 1 cut(s) 203
NaeI GCCGGC 1 cut(s) 253
NciI CCSGG 1 cut(s) 160
NdeII GATC 3 cut(s) 4, 63, 80
NgoMIV GCCGGC 1 cut(s) 251
NlaIII CATG 3 cut(s) 90, 268, 299
NlaIV GGNNCC 4 cut(s) 11, 174, 225, 240
NmuCI GTSAC 1 cut(s) 22
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 194
PdiI GCCGGC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 57
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 5 cut(s) 110, 141, 157, 344, 352
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
PpuMI RGGWCCY 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 4 cut(s) 11, 174, 225, 240
PspPI GGNCC 1 cut(s) 224
PspPPI RGGWCCY 1 cut(s) 224
PsuI RGATCY 1 cut(s) 80
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 300
SatI GCNGC 5 cut(s) 109, 140, 156, 343, 351
Sau3AI GATC 3 cut(s) 4, 63, 80
Sau96I GGNCC 1 cut(s) 224
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 2 cut(s) 160, 236
SduI GDGCHC 1 cut(s) 95
SetI ASST 5 cut(s) 24, 77, 171, 229, 234
SfaNI GCATC 3 cut(s) 103, 115, 367
SinI GGWCC 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 300
SsiI CCGC 4 cut(s) 50, 140, 204, 242
StyD4I CCNGG 2 cut(s) 158, 234
StyI CCWWGG 1 cut(s) 318
TaiI ACGT 3 cut(s) 24, 77, 234
TaqI TCGA 4 cut(s) 15, 123, 132, 375
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 1 cut(s) 57
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 4 cut(s) 108, 155, 342, 350
Tsp45I GTSAC 1 cut(s) 22
TspGWI ACGGA 1 cut(s) 41
TspRI CASTG 1 cut(s) 40
Tth111I GACNNNGTC 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 224
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.