Rw2G041460

CYSTM1 family protein A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005236
Physical Location & Seq
Reverse (-)
67462766 .. 67463373
608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G041460.1

Sequence Viewer

Length: 222 bp
ATGAGTTCAAGTCCTAACTCCGCAGCTGTGTACACCGCTCCGGCTTCTCAACAGGGACCTTACGTCCAGGCGCCGCCTCCTGCAGGCTATCCCACTATGAGAAACGGCTCCGACTTGCCCGGGAGCGACGGTCGTGTCGAAACCAAGCCGAAGGGCGATGGCTTCTGGAAGGGTTGCTGTGCCGCCTTGTGTTGCTGTTGTATGTTGGATGCTTGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

73

Amino Acids

7.52

Weight (kDa)

5.94

Isoelectric Point (pI)

54.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 21 - 73 6e-11 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 70
AccBSI CCGCTC 1 cut(s) 38
AciI CCGC 4 cut(s) 21, 36, 74, 183
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 1 cut(s) 9
AhdI GACNNNNNGTC 1 cut(s) 62
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 1 cut(s) 26
AluI AGCT 1 cut(s) 26
Ama87I CYCGRG 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 56
AsuC2I CCSGG 2 cut(s) 120, 121
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 1 cut(s) 56
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 35
BccI CCATC 1 cut(s) 152
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 1 cut(s) 68
BcnI CCSGG 2 cut(s) 120, 121
BfmI CTRYAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 74
BisI GCNGC 3 cut(s) 24, 74, 183
BlsI GCNGC 3 cut(s) 25, 75, 184
Bme1390I CCNGG 3 cut(s) 68, 120, 121
Bme18I GGWCC 1 cut(s) 56
BmeRI GACNNNNNGTC 1 cut(s) 62
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 3 cut(s) 57, 72, 109
BmrFI CCNGG 3 cut(s) 68, 120, 121
BmsI GCATC 1 cut(s) 199
BpuMI CCSGG 2 cut(s) 120, 121
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 119
BsaXI ACNNNNNCTCC 2 cut(s) 115, 145
Bsc4I CCNNNNNNNGG 1 cut(s) 83
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 119
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 1 cut(s) 83
BseXI GCAGC 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 133
BshNI GGYRCC 1 cut(s) 70
BsiEI CGRYCG 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 2 cut(s) 41, 120
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 69
BsoBI CYCGRG 1 cut(s) 119
Bsp1407I TGTACA 1 cut(s) 30
BspACI CCGC 4 cut(s) 21, 36, 74, 183
BspLI GGNNCC 3 cut(s) 57, 72, 109
BspMAI CTGCAG 1 cut(s) 85
BspT107I GGYRCC 1 cut(s) 70
BsrBI CCGCTC 1 cut(s) 38
BsrGI TGTACA 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 119
BssNI GRCGYC 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 71
BstAUI TGTACA 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 85, 214
BstENI CCTNNNNNAGG 1 cut(s) 81
BstF5I GGATG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 1 cut(s) 73
BstMCI CGRYCG 1 cut(s) 133
BstNI CCWGG 1 cut(s) 68
BstSCI CCNGG 3 cut(s) 66, 118, 119
BstSFI CTRYAG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 35
BtgZI GCGATG 1 cut(s) 171
BtsCI GGATG 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 85, 214
CfoI GCGC 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 56
Cfr9I CCCGGG 1 cut(s) 119
Csp6I GTAC 1 cut(s) 31
CviJI RGCY 6 cut(s) 26, 44, 87, 108, 148, 162
CviKI_1 RGCY 6 cut(s) 26, 44, 87, 108, 148, 162
CviQI GTAC 1 cut(s) 31
DinI GGCGCC 1 cut(s) 72
DrdI GACNNNNNNGTC 1 cut(s) 134
DriI GACNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 134
Eam1105I GACNNNNNGTC 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 56
Eco88I CYCGRG 1 cut(s) 119
EcoNI CCTNNNNNAGG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 66
EgeI GGCGCC 1 cut(s) 72
EheI GGCGCC 1 cut(s) 72
FaiI YATR 2 cut(s) 98, 203
FaqI GGGAC 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 24, 74, 183
Fsp4HI GCNGC 3 cut(s) 24, 74, 183
GlaI GCGC 1 cut(s) 72
GluI GCNGC 3 cut(s) 24, 74, 183
HaeII RGCGCY 1 cut(s) 74
HapII CCGG 2 cut(s) 41, 120
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 71
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HpaII CCGG 2 cut(s) 41, 120
Hpy166II GTNNAC 2 cut(s) 31, 33
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 1 cut(s) 166
Hpy8I GTNNAC 2 cut(s) 31, 33
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 145, 163
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 1 cut(s) 83
HpySE526I ACGT 1 cut(s) 63
Hsp92I GRCGYC 1 cut(s) 71
HspAI GCGC 1 cut(s) 71
KasI GGCGCC 1 cut(s) 70
LmnI GCTCC 3 cut(s) 43, 113, 123
LpnPI CCDG 8 cut(s) 38, 53, 54, 69, 80, 93, 133, 151
Lsp1109I GCAGC 1 cut(s) 35
LweI GCATC 1 cut(s) 199
MaeII ACGT 1 cut(s) 63
MbiI CCGCTC 1 cut(s) 38
Mly113I GGCGCC 1 cut(s) 71
MmeI TCCRAC 2 cut(s) 135, 186
MnlI CCTC 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 2 cut(s) 41, 120
MspR9I CCNGG 3 cut(s) 68, 120, 121
MvaI CCWGG 1 cut(s) 68
NarI GGCGCC 1 cut(s) 71
NciI CCSGG 2 cut(s) 120, 121
NlaIV GGNNCC 3 cut(s) 57, 72, 109
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PkrI GCNGC 3 cut(s) 25, 75, 184
PluTI GGCGCC 1 cut(s) 74
PpuMI RGGWCCY 1 cut(s) 56
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 3 cut(s) 57, 72, 109
PspPI GGNCC 1 cut(s) 56
PspPPI RGGWCCY 1 cut(s) 56
PstI CTGCAG 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SatI GCNGC 3 cut(s) 24, 74, 183
Sau96I GGNCC 1 cut(s) 56
SbfI CCTGCAGG 1 cut(s) 85
ScrFI CCNGG 3 cut(s) 68, 120, 121
SdaI CCTGCAGG 1 cut(s) 85
SetI ASST 3 cut(s) 28, 61, 66
SfaNI GCATC 1 cut(s) 199
SfcI CTRYAG 1 cut(s) 81
SfoI GGCGCC 1 cut(s) 72
SinI GGWCC 1 cut(s) 56
SmaI CCCGGG 1 cut(s) 121
Sse8387I CCTGCAGG 1 cut(s) 85
SsiI CCGC 4 cut(s) 21, 36, 74, 183
SspDI GGCGCC 1 cut(s) 70
StyD4I CCNGG 3 cut(s) 66, 118, 119
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 1 cut(s) 66
TaqI TCGA 1 cut(s) 138
TatI WGTACW 1 cut(s) 30
TauI GCSGC 2 cut(s) 76, 185
TseI GCWGC 1 cut(s) 23
TspMI CCCGGG 1 cut(s) 119
VpaK11BI GGWCC 1 cut(s) 56
XagI CCTNNNNNAGG 1 cut(s) 81
XmaI CCCGGG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.