Rmu_sc0000758.1_g000009

CYSTM1 family protein A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000758.1
OG0005236
Physical Location & Seq
Reverse (-)
36022 .. 40276
4255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000758.1_g000009.1.cds

Sequence Viewer

Length: 735 bp
atggaggattcaagtgctgcacaaggtgttgaggaaggccatgagcagaatatgcataaattcaagttgctgaggcaagaaaaagatgtcgtcgagataaaaaatgaggtggacaagtacttactagaatctttagaagatccgtccaacgacaagtttgatgttcttgcctggtcatctctcaatccaagaatggttgaagctttggtctgcatgagggactgtttaagggctgcaggctttaatgcttataaggagcctactaaagatgaattggatatatacaatgagctggaaaagatggagaatgatcggagaaatcaaatcagtcactggagggattggcttcatcgaccacggtcagagtctcctccatcttccgctgcgactagattgtcatccttgtcgtccacaagatctgtagcttttgtggtcgccggaagttggccagaggtctgtcggagacccaccgaagttggccggaggtcggccggattcatatcatctatcatcatgagttcaagtcctaactccgcagctgtgtacaccgctccggcttctcaacagggaccttacgtcctggcgccgcctcctgcaggctatcccaccatgagagacggctccgacttgcccgggagcgacggtcgtgtcgaaaccaagccgaagggcgatggcttctggaagggttgctgtgccgccttgtgttgctgttgtatgttggatgcttgcttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

244

Amino Acids

27.08

Weight (kDa)

5.37

Isoelectric Point (pI)

63.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 252
AasI GACNNNNNNGTC 2 cut(s) 394, 647
AccB1I GGYRCC 1 cut(s) 583
AccBSI CCGCTC 1 cut(s) 551
AciI CCGC 5 cut(s) 381, 534, 549, 587, 696
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 3 cut(s) 446, 478, 489
AcsI RAATTY 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 584
AdeI CACNNNGTG 1 cut(s) 26
AfaI GTAC 2 cut(s) 119, 545
AfiI CCNNNNNNNGG 3 cut(s) 444, 487, 596
AgsI TTSAA 4 cut(s) 12, 64, 200, 522
AhdI GACNNNNNGTC 1 cut(s) 575
AjnI CCWGG 2 cut(s) 170, 579
AluBI AGCT 4 cut(s) 203, 292, 425, 539
AluI AGCT 4 cut(s) 203, 292, 425, 539
Alw26I GTCTC 3 cut(s) 372, 457, 609
AlwI GGATC 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 333
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 4 cut(s) 37, 446, 478, 489
ApeKI GCWGC 4 cut(s) 17, 233, 383, 536
ApoI RAATTY 1 cut(s) 59
AspLEI GCGC 1 cut(s) 586
AspS9I GGNCC 1 cut(s) 569
AsuC2I CCSGG 2 cut(s) 633, 634
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 1 cut(s) 569
BalI TGGCCA 1 cut(s) 448
BanI GGYRCC 1 cut(s) 583
BbvCI CCTCAGC 1 cut(s) 71
BbvI GCAGC 4 cut(s) 4, 220, 370, 548
BccI CCATC 3 cut(s) 295, 382, 665
BceAI ACGGC 1 cut(s) 634
BciT130I CCWGG 2 cut(s) 172, 581
BcnI CCSGG 2 cut(s) 633, 634
BcoDI GTCTC 3 cut(s) 372, 457, 609
BfaI CTAG 2 cut(s) 125, 390
BfmI CTRYAG 3 cut(s) 234, 420, 594
BfoI RGCGCY 1 cut(s) 587
BglII AGATCT 1 cut(s) 416
BisI GCNGC 6 cut(s) 18, 234, 384, 537, 587, 696
BlsI GCNGC 6 cut(s) 19, 235, 385, 538, 588, 697
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 4 cut(s) 172, 581, 633, 634
Bme18I GGWCC 1 cut(s) 569
BmeRI GACNNNNNGTC 1 cut(s) 575
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 1 cut(s) 569
BmiI GGNNCC 4 cut(s) 258, 570, 585, 622
BmrFI CCNGG 4 cut(s) 172, 581, 633, 634
BmsI GCATC 1 cut(s) 712
BoxI GACNNNNGTC 1 cut(s) 358
BpmI CTGGAG 1 cut(s) 355
Bpu10I CCTNAGC 1 cut(s) 71
BpuMI CCSGG 2 cut(s) 633, 634
BsaBI GATNNNNATC 2 cut(s) 397, 499
BsaHI GRCGYC 1 cut(s) 584
BsaI GGTCTC 1 cut(s) 457
BsaJI CCNNGG 2 cut(s) 356, 632
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 3 cut(s) 444, 487, 596
Bse1I ACTGG 1 cut(s) 338
Bse8I GATNNNNATC 2 cut(s) 397, 499
BseBI CCWGG 2 cut(s) 172, 581
BseDI CCNNGG 2 cut(s) 356, 632
BseGI GGATG 2 cut(s) 398, 727
BseJI GATNNNNATC 2 cut(s) 397, 499
BseLI CCNNNNNNNGG 3 cut(s) 444, 487, 596
BseMII CTCAG 1 cut(s) 62
BseNI ACTGG 1 cut(s) 338
BseRI GAGGAG 1 cut(s) 360
BseX3I CGGCCG 1 cut(s) 489
BseXI GCAGC 4 cut(s) 4, 220, 370, 548
Bsh1285I CGRYCG 2 cut(s) 492, 646
BshFI GGCC 4 cut(s) 39, 448, 480, 491
BshNI GGYRCC 1 cut(s) 583
BsiEI CGRYCG 2 cut(s) 492, 646
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 5 cut(s) 438, 481, 492, 554, 633
BslFI GGGAC 2 cut(s) 233, 582
BslI CCNNNNNNNGG 3 cut(s) 444, 487, 596
BsmAI GTCTC 3 cut(s) 372, 457, 609
BsmBI CGTCTC 1 cut(s) 609
BsmFI GGGAC 2 cut(s) 233, 582
BsnI GGCC 4 cut(s) 39, 448, 480, 491
Bso31I GGTCTC 1 cut(s) 457
BsoBI CYCGRG 1 cut(s) 632
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 3 cut(s) 139, 310, 416
BspACI CCGC 5 cut(s) 381, 534, 549, 587, 696
BspANI GGCC 4 cut(s) 39, 448, 480, 491
BspCNI CTCAG 1 cut(s) 63
BspHI TCATGA 1 cut(s) 513
BspLI GGNNCC 4 cut(s) 258, 570, 585, 622
BspMAI CTGCAG 2 cut(s) 238, 598
BspPI GGATC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 583
BspTNI GGTCTC 1 cut(s) 457
BsrBI CCGCTC 1 cut(s) 551
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 1 cut(s) 338
BssECI CCNNGG 2 cut(s) 356, 632
BssMI GATC 3 cut(s) 139, 310, 416
BssNI GRCGYC 1 cut(s) 584
Bst2UI CCWGG 2 cut(s) 172, 581
Bst4CI ACNGT 3 cut(s) 224, 360, 644
BstACI GRCGYC 1 cut(s) 584
BstAPI GCANNNNNTGC 1 cut(s) 52
BstAUI TGTACA 1 cut(s) 543
BstC8I GCNNGC 3 cut(s) 238, 598, 727
BstDEI CTNAG 1 cut(s) 71
BstDSI CCRYGG 1 cut(s) 356
BstENI CCTNNNNNAGG 1 cut(s) 594
BstF5I GGATG 2 cut(s) 398, 727
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 1 cut(s) 586
BstKTI GATC 3 cut(s) 142, 313, 419
BstMAI GTCTC 3 cut(s) 372, 457, 609
BstMBI GATC 3 cut(s) 139, 310, 416
BstMCI CGRYCG 2 cut(s) 492, 646
BstMWI GCNNNNNNNGC 1 cut(s) 52
BstNI CCWGG 2 cut(s) 172, 581
BstPAI GACNNNNGTC 1 cut(s) 358
BstSCI CCNGG 4 cut(s) 170, 579, 631, 632
BstSFI CTRYAG 3 cut(s) 234, 420, 594
BstV1I GCAGC 4 cut(s) 4, 220, 370, 548
BstX2I RGATCY 2 cut(s) 139, 416
BstYI RGATCY 2 cut(s) 139, 416
BstZI CGGCCG 1 cut(s) 489
BsuRI GGCC 4 cut(s) 39, 448, 480, 491
BtgI CCRYGG 1 cut(s) 356
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 2 cut(s) 398, 727
BtsIMutI CAGTG 1 cut(s) 331
Cac8I GCNNGC 3 cut(s) 238, 598, 727
CaiI CAGNNNCTG 1 cut(s) 333
CciI TCATGA 1 cut(s) 513
CfoI GCGC 1 cut(s) 586
Cfr13I GGNCC 1 cut(s) 569
Cfr9I CCCGGG 1 cut(s) 632
Csp6I GTAC 2 cut(s) 118, 544
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 4 cut(s) 41, 214, 514, 610
CviQI GTAC 2 cut(s) 118, 544
DdeI CTNAG 1 cut(s) 71
DinI GGCGCC 1 cut(s) 585
DpnI GATC 3 cut(s) 141, 312, 418
DpnII GATC 3 cut(s) 139, 310, 416
DraIII CACNNNGTG 1 cut(s) 26
DrdI GACNNNNNNGTC 2 cut(s) 394, 647
DriI GACNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 2 cut(s) 394, 647
EaeI YGGCCR 3 cut(s) 446, 478, 489
EagI CGGCCG 1 cut(s) 489
Eam1105I GACNNNNNGTC 1 cut(s) 575
EclXI CGGCCG 1 cut(s) 489
Eco31I GGTCTC 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 569
Eco52I CGGCCG 1 cut(s) 489
Eco88I CYCGRG 1 cut(s) 632
EcoNI CCTNNNNNAGG 1 cut(s) 594
EcoO109I RGGNCCY 1 cut(s) 569
EcoRII CCWGG 2 cut(s) 170, 579
EcoT22I ATGCAT 1 cut(s) 57
EgeI GGCGCC 1 cut(s) 585
EheI GGCGCC 1 cut(s) 585
Esp3I CGTCTC 1 cut(s) 609
FaeI CATG 4 cut(s) 44, 217, 517, 613
FaqI GGGAC 2 cut(s) 233, 582
FatI CATG 4 cut(s) 40, 213, 513, 609
Fnu4HI GCNGC 6 cut(s) 18, 234, 384, 537, 587, 696
FokI GGATG 1 cut(s) 385
Fsp4HI GCNGC 6 cut(s) 18, 234, 384, 537, 587, 696
FspBI CTAG 2 cut(s) 125, 390
GlaI GCGC 1 cut(s) 585
GluI GCNGC 6 cut(s) 18, 234, 384, 537, 587, 696
GsuI CTGGAG 1 cut(s) 355
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 4 cut(s) 39, 448, 480, 491
HapII CCGG 5 cut(s) 438, 481, 492, 554, 633
HhaI GCGC 1 cut(s) 586
Hin1I GRCGYC 1 cut(s) 584
Hin1II CATG 4 cut(s) 44, 217, 517, 613
Hin6I GCGC 1 cut(s) 584
HinP1I GCGC 1 cut(s) 584
HindIII AAGCTT 1 cut(s) 201
HinfI GANTC 4 cut(s) 8, 128, 365, 495
HpaII CCGG 5 cut(s) 438, 481, 492, 554, 633
Hpy166II GTNNAC 4 cut(s) 112, 411, 544, 546
Hpy188I TCNGA 4 cut(s) 315, 364, 462, 625
Hpy188III TCNNGA 3 cut(s) 94, 514, 679
Hpy8I GTNNAC 4 cut(s) 112, 411, 544, 546
Hpy99I CGWCG 2 cut(s) 95, 644
HpyAV CCTTC 3 cut(s) 29, 658, 676
HpyCH4III ACNGT 3 cut(s) 224, 360, 644
HpyCH4IV ACGT 1 cut(s) 576
HpyCH4V TGCA 5 cut(s) 20, 55, 213, 236, 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 52
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 1 cut(s) 576
Hsp92I GRCGYC 1 cut(s) 584
Hsp92II CATG 4 cut(s) 44, 217, 517, 613
HspAI GCGC 1 cut(s) 584
KasI GGCGCC 1 cut(s) 583
Kzo9I GATC 3 cut(s) 139, 310, 416
LmnI GCTCC 4 cut(s) 256, 556, 626, 636
Lsp1109I GCAGC 4 cut(s) 4, 220, 370, 548
LweI GCATC 1 cut(s) 712
MaeI CTAG 2 cut(s) 125, 390
MaeII ACGT 1 cut(s) 576
MaeIII GTNAC 1 cut(s) 329
MalI GATC 3 cut(s) 141, 312, 418
MbiI CCGCTC 1 cut(s) 551
MboI GATC 3 cut(s) 139, 310, 416
MboII GAAGA 2 cut(s) 149, 369
MflI RGATCY 2 cut(s) 139, 416
MlsI TGGCCA 1 cut(s) 448
MluCI AATT 2 cut(s) 59, 272
MluNI TGGCCA 1 cut(s) 448
Mly113I GGCGCC 1 cut(s) 584
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 4 cut(s) 171, 440, 648, 699
MnlI CCTC 9 cut(s) 25, 66, 100, 210, 330, 381, 445, 477, 600
Mox20I TGGCCA 1 cut(s) 448
Mph1103I ATGCAT 1 cut(s) 57
MscI TGGCCA 1 cut(s) 448
MseI TTAA 2 cut(s) 227, 243
Msp20I TGGCCA 1 cut(s) 448
MspA1I CMGCKG 2 cut(s) 383, 539
MspI CCGG 5 cut(s) 438, 481, 492, 554, 633
MspR9I CCNGG 4 cut(s) 172, 581, 633, 634
MvaI CCWGG 2 cut(s) 172, 581
MwoI GCNNNNNNNGC 1 cut(s) 52
NarI GGCGCC 1 cut(s) 584
NciI CCSGG 2 cut(s) 633, 634
NdeII GATC 3 cut(s) 139, 310, 416
NlaIII CATG 4 cut(s) 44, 217, 517, 613
NlaIV GGNNCC 4 cut(s) 258, 570, 585, 622
NmuCI GTSAC 1 cut(s) 329
NsiI ATGCAT 1 cut(s) 57
PagI TCATGA 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 648
PfeI GAWTC 3 cut(s) 8, 128, 495
PkrI GCNGC 6 cut(s) 19, 235, 385, 538, 588, 697
PleI GAGTC 1 cut(s) 373
PluTI GGCGCC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 373
PpuMI RGGWCCY 1 cut(s) 569
PshAI GACNNNNGTC 1 cut(s) 358
PsiI TTATAA 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 569
Psp6I CCWGG 2 cut(s) 170, 579
PspGI CCWGG 2 cut(s) 170, 579
PspN4I GGNNCC 4 cut(s) 258, 570, 585, 622
PspPI GGNCC 1 cut(s) 569
PspPPI RGGWCCY 1 cut(s) 569
PstI CTGCAG 2 cut(s) 238, 598
PstNI CAGNNNCTG 1 cut(s) 333
PsuI RGATCY 2 cut(s) 139, 416
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 2 cut(s) 119, 545
RsaNI GTAC 2 cut(s) 118, 544
SaqAI TTAA 2 cut(s) 227, 243
SatI GCNGC 6 cut(s) 18, 234, 384, 537, 587, 696
Sau3AI GATC 3 cut(s) 139, 310, 416
Sau96I GGNCC 1 cut(s) 569
SbfI CCTGCAGG 1 cut(s) 598
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 4 cut(s) 172, 581, 633, 634
SdaI CCTGCAGG 1 cut(s) 598
SfaNI GCATC 1 cut(s) 712
SfcI CTRYAG 3 cut(s) 234, 420, 594
SfoI GGCGCC 1 cut(s) 585
SinI GGWCC 1 cut(s) 569
SmaI CCCGGG 1 cut(s) 634
Sse8387I CCTGCAGG 1 cut(s) 598
Sse9I AATT 2 cut(s) 59, 272
SsiI CCGC 5 cut(s) 381, 534, 549, 587, 696
SspDI GGCGCC 1 cut(s) 583
SspMI CTAG 2 cut(s) 125, 390
StyD4I CCNGG 4 cut(s) 170, 579, 631, 632
TaaI ACNGT 3 cut(s) 224, 360, 644
TaiI ACGT 1 cut(s) 579
TaqI TCGA 3 cut(s) 93, 352, 651
TasI AATT 2 cut(s) 59, 272
TatI WGTACW 2 cut(s) 117, 543
TauI GCSGC 2 cut(s) 589, 698
TfiI GAWTC 3 cut(s) 8, 128, 495
Tru1I TTAA 2 cut(s) 227, 243
Tru9I TTAA 2 cut(s) 227, 243
TscAI CASTG 1 cut(s) 338
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 4 cut(s) 17, 233, 383, 536
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 3 cut(s) 285, 338, 487
TspGWI ACGGA 1 cut(s) 132
TspMI CCCGGG 1 cut(s) 632
TspRI CASTG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 569
XagI CCTNNNNNAGG 1 cut(s) 594
XapI RAATTY 1 cut(s) 59
XmaI CCCGGG 1 cut(s) 632
XspI CTAG 2 cut(s) 125, 390
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.