Rh2BG071300

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005236
Physical Location & Seq
Forward (+)
5382623 .. 5386757
4135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG071300.1

Sequence Viewer

Length: 537 bp
ATGGATCGGAGCTCCGACGACGTCGCGGCAAGTGGTGATGATGACGATGCGAAAGGATTCTGGATCAGGTTGACGCGGAGATCTACATGGCGCTCTACATCGCCTCGCCGCGATGCTGTTGTCGATGCGTTCGAGTTGCCGCAGGAGACGATCACTGCCAGGGACGAGGTGGCACCGAGTTCGGCGCCGGCGAAAATGGCAAGTCAAAGAATCACACCGCTTAGAACTAGCTGCTGCGCGGTGAACGAAGCAGACATGCCCAGGAACATGGCTGCTACCTTCCGTAAATACCTATTGGCAGAGTGGTCTAGTCTTCCTCAAGTCATTCTCTCAGAGATTCTGGATAAGTTGTTGGAACCAACTGACCATGTTCGTTTTGCTGCCGTTTGCAAGGAATGGTATGCTCTCGCAACGGACTATAACCACACAACCCAGAGTTGTTTTAAGGTGCATCCCATGCTTATGATCCCTTCAGAATCCCAAGGAAAGCGAAAACTATACAGCATCTCTGAGCAAAAGGTCTACAACAAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

178

Amino Acids

20.16

Weight (kDa)

6.83

Isoelectric Point (pI)

58.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 102 - 139 8.7e-09 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AccB1I GGYRCC 2 cut(s) 172, 184
AccI GTMKAC 1 cut(s) 522
AccII CGCG 4 cut(s) 26, 76, 111, 239
AciI CCGC 6 cut(s) 26, 76, 109, 140, 218, 239
AclWI GGATC 3 cut(s) 12, 71, 460
AcuI CTGAAG 1 cut(s) 456
AcyI GRCGYC 2 cut(s) 21, 185
AjnI CCWGG 2 cut(s) 158, 260
AluBI AGCT 2 cut(s) 12, 231
AluI AGCT 2 cut(s) 12, 231
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 3 cut(s) 12, 71, 460
ApeKI GCWGC 4 cut(s) 231, 234, 272, 380
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 3 cut(s) 93, 187, 239
AsuHPI GGTGA 2 cut(s) 47, 253
BanI GGYRCC 2 cut(s) 172, 184
BanII GRGCYC 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 305
Bbv12I GWGCWC 1 cut(s) 14
BbvI GCAGC 4 cut(s) 218, 221, 259, 367
BceAI ACGGC 1 cut(s) 368
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 2 cut(s) 160, 262
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 2 cut(s) 228, 309
BfoI RGCGCY 2 cut(s) 94, 188
BglII AGATCT 1 cut(s) 80
BisI GCNGC 7 cut(s) 27, 109, 140, 232, 235, 273, 381
BlsI GCNGC 7 cut(s) 28, 110, 141, 233, 236, 274, 382
Bme1390I CCNGG 2 cut(s) 160, 262
BmiI GGNNCC 3 cut(s) 174, 186, 357
BmrFI CCNGG 2 cut(s) 160, 262
BmsI GCATC 5 cut(s) 37, 103, 115, 460, 513
BpiI GAAGAC 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 303
BsaHI GRCGYC 2 cut(s) 21, 185
BsaJI CCNNGG 3 cut(s) 159, 260, 481
Bse118I RCCGGY 1 cut(s) 187
BseBI CCWGG 2 cut(s) 160, 262
BseDI CCNNGG 3 cut(s) 159, 260, 481
BseGI GGATG 1 cut(s) 451
BseMII CTCAG 2 cut(s) 345, 501
BseXI GCAGC 4 cut(s) 218, 221, 259, 367
Bsh1236I CGCG 4 cut(s) 26, 76, 111, 239
BshNI GGYRCC 2 cut(s) 172, 184
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 1 cut(s) 188
BslFI GGGAC 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 140
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 5 cut(s) 4, 63, 80, 150, 465
BspACI CCGC 6 cut(s) 26, 76, 109, 140, 218, 239
BspCNI CTCAG 2 cut(s) 344, 502
BspFNI CGCG 4 cut(s) 26, 76, 111, 239
BspLI GGNNCC 3 cut(s) 174, 186, 357
BspPI GGATC 3 cut(s) 12, 71, 460
BspT107I GGYRCC 2 cut(s) 172, 184
BsrFI RCCGGY 1 cut(s) 187
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 3 cut(s) 159, 260, 481
BssMI GATC 5 cut(s) 4, 63, 80, 150, 465
BssNI GRCGYC 2 cut(s) 21, 185
BssT1I CCWWGG 1 cut(s) 481
Bst2UI CCWGG 2 cut(s) 160, 262
BstACI GRCGYC 2 cut(s) 21, 185
BstAPI GCANNNNNTGC 1 cut(s) 457
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 3 cut(s) 221, 331, 510
BstF5I GGATG 1 cut(s) 451
BstFNI CGCG 4 cut(s) 26, 76, 111, 239
BstH2I RGCGCY 2 cut(s) 94, 188
BstHHI GCGC 3 cut(s) 93, 187, 239
BstKTI GATC 5 cut(s) 7, 66, 83, 153, 468
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 5 cut(s) 4, 63, 80, 150, 465
BstMWI GCNNNNNNNGC 2 cut(s) 197, 457
BstNI CCWGG 2 cut(s) 160, 262
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 2 cut(s) 158, 260
BstUI CGCG 4 cut(s) 26, 76, 111, 239
BstV1I GCAGC 4 cut(s) 218, 221, 259, 367
BstV2I GAAGAC 1 cut(s) 305
BstX2I RGATCY 1 cut(s) 80
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 1 cut(s) 80
BtgZI GCGATG 2 cut(s) 84, 126
BtsCI GGATG 1 cut(s) 451
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 189
CfoI GCGC 3 cut(s) 93, 187, 239
Cfr10I RCCGGY 1 cut(s) 187
CseI GACGC 1 cut(s) 82
CviAII CATG 5 cut(s) 87, 256, 268, 368, 457
CviJI RGCY 3 cut(s) 12, 231, 272
CviKI_1 RGCY 3 cut(s) 12, 231, 272
DdeI CTNAG 3 cut(s) 221, 331, 510
DinI GGCGCC 1 cut(s) 186
DpnI GATC 5 cut(s) 6, 65, 82, 152, 467
DpnII GATC 5 cut(s) 4, 63, 80, 150, 465
Ecl136II GAGCTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 481
Eco24I GRGCYC 1 cut(s) 14
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 456
EcoICRI GAGCTC 1 cut(s) 12
EcoRII CCWGG 2 cut(s) 158, 260
EcoT14I CCWWGG 1 cut(s) 481
EcoT38I GRGCYC 1 cut(s) 14
EgeI GGCGCC 1 cut(s) 186
EheI GGCGCC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 481
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 5 cut(s) 90, 259, 271, 371, 460
FaiI YATR 9 cut(s) 88, 257, 269, 369, 402, 420, 458, 464, 499
FaqI GGGAC 1 cut(s) 176
FatI CATG 5 cut(s) 86, 255, 267, 367, 456
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 7 cut(s) 27, 109, 140, 232, 235, 273, 381
FokI GGATG 1 cut(s) 438
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 7 cut(s) 27, 109, 140, 232, 235, 273, 381
FspBI CTAG 2 cut(s) 228, 309
GlaI GCGC 3 cut(s) 92, 186, 238
GluI GCNGC 7 cut(s) 27, 109, 140, 232, 235, 273, 381
HaeII RGCGCY 2 cut(s) 94, 188
HapII CCGG 1 cut(s) 188
HgaI GACGC 1 cut(s) 82
HhaI GCGC 3 cut(s) 93, 187, 239
Hin1I GRCGYC 2 cut(s) 21, 185
Hin1II CATG 5 cut(s) 90, 259, 271, 371, 460
Hin6I GCGC 3 cut(s) 91, 185, 237
HinP1I GCGC 3 cut(s) 91, 185, 237
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HinfI GANTC 4 cut(s) 57, 210, 337, 476
HpaII CCGG 1 cut(s) 188
HphI GGTGA 2 cut(s) 47, 253
Hpy166II GTNNAC 3 cut(s) 72, 244, 523
Hpy188I TCNGA 5 cut(s) 9, 16, 334, 475, 511
Hpy188III TCNNGA 2 cut(s) 61, 341
Hpy8I GTNNAC 3 cut(s) 72, 244, 523
Hpy99I CGWCG 3 cut(s) 20, 23, 26
HpyAV CCTTC 2 cut(s) 289, 480
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 2 cut(s) 390, 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 457
HpyF3I CTNAG 3 cut(s) 221, 331, 510
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 2 cut(s) 21, 185
Hsp92II CATG 5 cut(s) 90, 259, 271, 371, 460
HspAI GCGC 3 cut(s) 91, 185, 237
KasI GGCGCC 1 cut(s) 184
KroI GCCGGC 1 cut(s) 187
KroNI GCCGGC 1 cut(s) 189
Kzo9I GATC 5 cut(s) 4, 63, 80, 150, 465
LmnI GCTCC 2 cut(s) 9, 17
Lsp1109I GCAGC 4 cut(s) 218, 221, 259, 367
LweI GCATC 5 cut(s) 37, 103, 115, 460, 513
MaeI CTAG 2 cut(s) 228, 309
MaeII ACGT 1 cut(s) 21
MalI GATC 5 cut(s) 6, 65, 82, 152, 467
MboI GATC 5 cut(s) 4, 63, 80, 150, 465
MboII GAAGA 1 cut(s) 305
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 1 cut(s) 14
Mly113I GGCGCC 1 cut(s) 185
MmeI TCCRAC 2 cut(s) 39, 333
MnlI CCTC 3 cut(s) 114, 160, 327
MreI CGCCGGCG 1 cut(s) 187
MroNI GCCGGC 1 cut(s) 187
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 1 cut(s) 444
MslI CAYNNNNRTG 1 cut(s) 461
MspI CCGG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 160, 262
MvaI CCWGG 2 cut(s) 160, 262
MvnI CGCG 4 cut(s) 26, 76, 111, 239
MwoI GCNNNNNNNGC 2 cut(s) 197, 457
NaeI GCCGGC 1 cut(s) 189
NarI GGCGCC 1 cut(s) 185
NdeII GATC 5 cut(s) 4, 63, 80, 150, 465
NgoMIV GCCGGC 1 cut(s) 187
NlaIII CATG 5 cut(s) 90, 259, 271, 371, 460
NlaIV GGNNCC 3 cut(s) 174, 186, 357
NspI RCATGY 1 cut(s) 259
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 188
PdiI GCCGGC 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 4 cut(s) 57, 210, 337, 476
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 7 cut(s) 28, 110, 141, 233, 236, 274, 382
PluTI GGCGCC 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 14
Psp6I CCWGG 2 cut(s) 158, 260
PspGI CCWGG 2 cut(s) 158, 260
PspN4I GGNNCC 3 cut(s) 174, 186, 357
PsuI RGATCY 1 cut(s) 80
PsyI GACNNNGTC 1 cut(s) 20
RseI CAYNNNNRTG 1 cut(s) 461
SacI GAGCTC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 7 cut(s) 27, 109, 140, 232, 235, 273, 381
Sau3AI GATC 5 cut(s) 4, 63, 80, 150, 465
ScrFI CCNGG 2 cut(s) 160, 262
SduI GDGCHC 1 cut(s) 14
SetI ASST 9 cut(s) 14, 24, 71, 171, 233, 281, 294, 450, 522
SfaNI GCATC 5 cut(s) 37, 103, 115, 460, 513
SfoI GGCGCC 1 cut(s) 186
SgrAI CRCCGGYG 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 318
SmoI CTYRAG 1 cut(s) 318
SsiI CCGC 6 cut(s) 26, 76, 109, 140, 218, 239
SspDI GGCGCC 1 cut(s) 184
SspMI CTAG 2 cut(s) 228, 309
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 158, 260
StyI CCWWGG 1 cut(s) 481
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 123, 132
TauI GCSGC 3 cut(s) 29, 111, 142
TfiI GAWTC 4 cut(s) 57, 210, 337, 476
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 1 cut(s) 160
TseI GCWGC 4 cut(s) 231, 234, 272, 380
TspGWI ACGGA 2 cut(s) 272, 428
TspRI CASTG 1 cut(s) 160
Tth111I GACNNNGTC 1 cut(s) 20
XceI RCATGY 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 522
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 2 cut(s) 228, 309
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.