Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0002769

Physical Location & Seq

Genomic Coordinates Forward (+)

2391478 .. 2396326

Gene Structure 4849 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000026356

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3534 bp

ATGAAGATGGTGATGAGATCAGTGACAATGGCCTTTATGCCCCTCTCAATGATACTCACTATCTGTGCTGGTCCTTTGCTTCTCAATGCCTTCATTTTGGTTGCTGAGGGAAATGAAAGTTTCGAGCACGAAGGTTATCTGTTAGCCGGAGCACTTTTTGTCTCGAAAACCATTGAATCCTTGTCACAGAGGCAGTGGTACTTCAGGTGCAGGCTTATTGGTTTGAAAGTTAGGTCTTTGCTGACAGCAGCCATTTACAAAAAGCAATTGAGATTATCTAATGCTGCTAAGTTGACACACTCCGGTGGGGAGATCATGAATTATGTTACTGTGGATGCTTATAGGATTGGAGAGTTTCCATTTTGGTTCCATCAGACTTGGACTACTAGCGTCCAGCTCTGTTTTTCGTTGGTAATTCTTTTCCGTGCAGTTGGCCTGGCTACATTTGCAGCCTTGGTGGTGATACTTTTGAGTGTGATTTGCAATGCTCCAGTTGCTAAGCTTCAACATAAGTTCCAGAGTAAGCTTATGAAGGCACAAGATGAAAGGCTCAAGGCTAGTTCTGAGGCTCTAGTGAACATGAAGGTCTTAAAGTTATATGCATGGGAAACACATTTCAAGAAGGCAATAGAAAAGTTAAGGAAGGAGGAGCACAAATGGTTATCTGCAATGCTGTTAAGAAGAGCATATAGTACTTATCTATTTTGGTCAACTCCTGTTTTGGTCTCTACTGCAACATTTGGGGCATGCTATTTTCTCAAAATTCCGTTACATGCAAATAATGTTTTCACTTTTATCTCAACTTTGAGACTTGTTCAGGACCCCATTCGAACTATACCTGATGTTTTTGCAGTTGTCATCCAAGCGAAGGTTGCATTTGGTCGTATTGTGAAATTCCTTGAGGCACCTGAGTTGCAAACTTCAAATGTCCGGAAGTGCAACATGAAGAATGTAGCTAACTCCATTGAAATTAGGTCAGCTAATTTTTCATGGGAAGAAAATTTATCCAAGTCCATACTGAGAAATATAAATTTAGAGGTTAGACATGGTGAAAAGGTGGCAATATGTGGTGAAGTCGGCTCAGGCAAATCAAGCCTTCTAGCTGCAATTCTTGGAGAGATTCCAAATGTGCAAGGAAATATTCAAGTTTATGGGAAGATTGCCTATGTTTCTCAAACCGCATGGATCCAGTCAGGGAGTATACAAGAAAATATTCTCTTTGGTTCTTGTATGGATAGTCAGAGATATCGAGAGACGCTTGAGCGATGCTCACTAGTAAAGGACCTTGAGCTGCTTCCCTATGGTGACCTTACTGAAATAGGGGAGAGAGGGGTGAATCTAAGTGGAGGTCAGAAGCAGCGGATCCAACTTGCACGCGCTCTCTATCAGGATGCAGATATATATCTCTTGGATGATCCATTCAGTGCCGTTGATGCACATACTGCCACAAACTTATTTAATGAATATGTAATGGAAGCACTTTCAGGCAAGACAGTACTACTTGTGACCCATCAAGTTGATTTCCTGCCTGCATTTGATTTCGTTTTGTTGATGATGGATGGGGAGATCCTACAAGCAGCTCCTTATCAGCATCTATTGGCTTTAAGCCAGGAATTCCAGGACCTTGTCAATGCACACAAAGAAACTGCTGGTTCTGAAAGGCTTTCAGATGTCACTTCTGCTCAAAATTCTGGAATATCATCTAGAGAGATTAAGAAGAGCTATGTTGAGAAGCCATTAAAAGAAAATAAAGGTGACCAATTGATTAAACTAGAAGAGAGAGAAACAGGAGACGCAGGTTTTAAGCCATACATACTGTATCTGAAACAGAACAAAGGGTTCCTGTACTTCTCAATTGCCGTTTGTCTCCACTTCACTTTTATTATGAGTCAGATTGCACAGAACTCTTGGATGGCTGCTAATGTTGATAATCCGAGTATTAGCACATTGCGGTTGCTTGTGGTTTACTTATCGATTGGATTCTCCGCAACATTTATTTTTCTGTTTAGATCTCTTGCAGCAGTTGTTGGGGGTCTTGAGACATCAAAGTCGTTGTTCTCACAACTACTAAACTCCCTTTTTCGTGCACCCATGTCATTCTATGACTCAACACCTCTGGGAAGGATACTTAGTCGTGTTTCATCTGATCTGAGTATCACAGATCTTGATATTCCATTCAGCATAGTATTTGCTTGTGGAGCTACCATGAATGCCTATTGCAATCTTGGAGTATTGACTGTTGTTACCTGGCAAGTCTTGTTTGTCTCCATACCAATGGTCTATGTCGCAATTCAGCTACAGAAATATTACTTTTCCACTGCAAAAGAGTTGATGAGGATCAATGGAACAACCAAGTCCTTTGTAGCAAACCATCTAGCCGAGTCTGTATCAGGAGCCATTACCATTAGAGCTTTCAATGAAGAAGAGCGCTTCTCGGCAAAGAGCTTTCACCTCATTGACACAAATGCTAGTCCTTATTTCCACAGCTTTTCCGCAAATGAGTGGTTAATCCAACGATTAGAGATGATCAGTGCATCGGTTCTTGCCTCTGCAGCACTTTGTATGGTTTTGCTTCCTCATGGAACCTTTACCTCTGGGTTTATTGGGATGGCACTTTCTTATGGTCTATCACTAAATGTGTCCTTGATCAACTCAATTCAGAACCAGTGCACTATAGCAAACTACATTATTTCTGTTGAAAGACTAAATCAATACATGCACATTCCAAGTGAAGCCCCGGAAGTAATAGAGGGAAACCGTCCACCGGCCAACTGGCCGGTTGTAGGAAAAGTGGAGATACAAAATTTACAGATAAGATATAGGGATGACACACCACTTGTTCTTCGAGGGATTAGTTGTGTTTTTGAAGGAGGACATAAGATTGGTATTGTTGGCCGGACAGGCAGTGGGAAGTCTACACTCATCGGTGCTCTATTTCGGCTGGTCGAGCCAACCGGAGGGAAGATCATAGTCGATGGCATTGACATCTGCACTATAGGTCTTCATGATCTGAGGTCAAGATTTGGAATAATACCTCAAGACCCTACTCTCTTTAATGGCACTGTCAGATACAATTTGGACCCCTTATCTCAACATTCAGACCAGAAAATATGGGAGGTTCTTGGGAAGTGTCAGCTTCGAGAGGCTGTTGAGGAGAAAGAAAAAGGCTTAGACTCGTTAGTTGTGGATGATGGATCAAACTGGAGCATGGGGCAAAGGCAACTGTTCTGCCTCGGGCGCGCTCTTCTAAGGAGAAGTCGGGTATTGGTGCTTGATGAAGCAACTGCATCCATCGACAATGCAACTGATACAATCCTGCAGAAGACTATAAGGACTGAGTTTGAAGCTTGTACAGTAATTACAGTAGCTCACAGGATACCAACTGTGATGGATTGCACAATGGTTCTTGCCATTAGTGATGGAAAGATAGTGGAGTATGATGAGCCAATGAGGTTGATGAAGAGAGAAGGCTCGCTGTTTGGGCAGCTTGTCAAGGAGTACTGGTCTCATCTCCAGTCTGCAGAATCTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0008150 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04976 map01523 map01524 map02010 map04976

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1178

Amino Acids

131.41

Weight (kDa)

8.09

Isoelectric Point (pI)

35.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	54 - 275	1.7e-22	ABC transporter transmembrane region
ABC_tran	PF00005	340 - 474	1.2e-22	ABC transporter
ABC_membrane	PF00664	616 - 874	7.7e-25	ABC transporter transmembrane region
ABC_tran	PF00005	948 - 1096	1.1e-24	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002769)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59140
fragaria_vesca	FvH4_3g44221 FvH4_3g44230 FvH4_3g44230 FvH4_7g30860 FvH4_7g30860 FvH4_7g30860 FvH4_7g30870 FvH4_7g30870 FvH4_7g30870 FvH4_7g30870 FvH4_7g30870
malus_domestica	MD01G1213600.v1.1
prunus_persica	Prupe.2G305800_v2.0.a1 Prupe.2G305800_v2.0.a1
pyrus_communis	pycom01g22440 pycom07g25750
rosa_chinensis	RchiOBHm_Chr1g0379791 RchiOBHm_Chr1g0379831 RchiOBHm_Chr1g0379841
rosa_laevigata	RLG00000026355 RLG00000026356 RLG00000026359 RLG00000026360
rosa_multiflora	Rmu_sc0014259.1_g000008 Rmu_sc0014259.1_g000011
rosa_roxburghii	Rroxscaffold_4G00279900 Rroxscaffold_4G00279910
rosa_rugosa	Rorug01G0415300
rosa_samantha	Rh1AG438800 Rh1AG439300 Rh1AG439400 Rh1BG395300 Rh1BG395400 Rh1DG426200 Rh1DG426300
rosa_wichuraiana	Rw1G038220 Rw1G038240 Rw1G038250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2047
Acc36I	ACCTGC	1 cut(s)	1787
AccB1I	GGYRCC	1 cut(s)	904
AccI	GTMKAC	2 cut(s)	1201, 2915
AccII	CGCG	2 cut(s)	1377, 3240
AccIII	TCCGGA	1 cut(s)	930
AciI	CCGC	5 cut(s)	1179, 1360, 1951, 1986, 2493
AclWI	GGATC	8 cut(s)	1180, 1193, 1357, 1370, 1409, 1561, 2345, 3202
AcoI	YGGCCR	3 cut(s)	2766, 2774, 2893
AcsI	RAATTY	7 cut(s)	762, 893, 1000, 1030, 1613, 1687, 2803
AcuI	CTGAAG	1 cut(s)	187
AfaI	GTAC	6 cut(s)	200, 694, 1497, 1847, 3352, 3500
AfeI	AGCGCT	1 cut(s)	2429
AfiI	CCNNNNNNNGG	4 cut(s)	868, 2764, 2783, 2957
AhlI	ACTAGT	1 cut(s)	1273
AjnI	CCWGG	4 cut(s)	435, 1608, 1617, 2246
AleI	CACNNNNGTG	1 cut(s)	303
Alw21I	GWGCWC	6 cut(s)	129, 154, 654, 2089, 2672, 2932
Alw26I	GTCTC	9 cut(s)	166, 730, 802, 1247, 1785, 1871, 2033, 2269, 3510
Alw44I	GTGCAC	2 cut(s)	2085, 2668
AlwI	GGATC	8 cut(s)	1180, 1193, 1357, 1370, 1409, 1561, 2345, 3202
Ama87I	CYCGRG	1 cut(s)	3233
Aor13HI	TCCGGA	1 cut(s)	930
Aor51HI	AGCGCT	1 cut(s)	2429
AoxI	GGCC	5 cut(s)	30, 433, 2766, 2774, 2893
ApaLI	GTGCAC	2 cut(s)	2085, 2668
ApoI	RAATTY	7 cut(s)	762, 893, 1000, 1030, 1613, 1687, 2803
Asp700I	GAANNNNTTC	1 cut(s)	1212
AspLEI	GCGC	4 cut(s)	1379, 2430, 3240, 3242
AspS9I	GGNCC	5 cut(s)	71, 820, 1282, 1621, 3079
AsuC2I	CCSGG	1 cut(s)	2738
AsuHPI	GGTGA	8 cut(s)	22, 472, 1061, 1082, 1316, 1345, 1766, 2441
AsuII	TTCGAA	1 cut(s)	829
AvaI	CYCGRG	1 cut(s)	3233
AvaII	GGWCC	5 cut(s)	71, 820, 1282, 1621, 3079
BaeGI	GKGCMC	2 cut(s)	2089, 2672
BamHI	GGATCC	2 cut(s)	1185, 1362
BanI	GGYRCC	1 cut(s)	904
BbsI	GAAGAC	2 cut(s)	2993, 3329
Bbv12I	GWGCWC	6 cut(s)	129, 154, 654, 2089, 2672, 2932
BbvCI	CCTCAGC	1 cut(s)	105
BceAI	ACGGC	2 cut(s)	1412, 1844
BciT130I	CCWGG	4 cut(s)	437, 1610, 1619, 2248
BciVI	GTATCC	2 cut(s)	2118, 3369
BclI	TGATCA	2 cut(s)	2526, 2646
BcnI	CCSGG	1 cut(s)	2738
BcoDI	GTCTC	9 cut(s)	166, 730, 802, 1247, 1785, 1871, 2033, 2269, 3510
BcuI	ACTAGT	1 cut(s)	1273
BfaI	CTAG	9 cut(s)	387, 558, 572, 1100, 1274, 1704, 1772, 2375, 2469
BfmI	CTRYAG	6 cut(s)	2297, 2550, 2673, 2994, 3317, 3519
BfoI	RGCGCY	1 cut(s)	2431
BfuAI	ACCTGC	1 cut(s)	1787
BfuI	GTATCC	2 cut(s)	2118, 3369
BglI	GCCNNNNNGGC	1 cut(s)	2901
BglII	AGATCT	2 cut(s)	2009, 2161
BlpI	GCTNAGC	1 cut(s)	498
BmcAI	AGTACT	3 cut(s)	694, 1497, 3500
Bme1390I	CCNGG	5 cut(s)	437, 1610, 1619, 2248, 2738
Bme18I	GGWCC	5 cut(s)	71, 820, 1282, 1621, 3079
BmeT110I	CYCGRG	1 cut(s)	3233
BmgT120I	GGNCC	5 cut(s)	71, 820, 1282, 1621, 3079
BmiI	GGNNCC	9 cut(s)	368, 822, 906, 1187, 1364, 1841, 2395, 2584, 3081
BmrFI	CCNGG	5 cut(s)	437, 1610, 1619, 2248, 2738
BmsI	GCATC	7 cut(s)	325, 1256, 1381, 1423, 1600, 2543, 3296
BpiI	GAAGAC	2 cut(s)	2993, 3329
BplI	GAGNNNNNCTC	4 cut(s)	1253, 1285, 2417, 2449
BpmI	CTGGAG	3 cut(s)	474, 3223, 3497
Bpu10I	CCTNAGC	2 cut(s)	105, 1081
Bpu1102I	GCTNAGC	1 cut(s)	498
Bpu14I	TTCGAA	1 cut(s)	829
BpuEI	CTTGAG	6 cut(s)	536, 920, 1280, 1307, 2057, 3021
BpuMI	CCSGG	1 cut(s)	2738
Bsa29I	ATCGAT	1 cut(s)	1973
BsaBI	GATNNNNATC	2 cut(s)	1401, 2336
BsaI	GGTCTC	2 cut(s)	730, 3510
BsaJI	CCNNGG	3 cut(s)	453, 2736, 3232
BsaWI	WCCGGW	3 cut(s)	302, 930, 2954
BsaXI	ACNNNNNCTCC	2 cut(s)	3488, 3518
Bsc4I	CCNNNNNNNGG	4 cut(s)	868, 2764, 2783, 2957
Bse118I	RCCGGY	2 cut(s)	2764, 2776
Bse1I	ACTGG	7 cut(s)	491, 1189, 2665, 2777, 3206, 3506, 3514
Bse3DI	GCAATG	3 cut(s)	490, 675, 1946
Bse8I	GATNNNNATC	2 cut(s)	1401, 2336
BseAI	TCCGGA	1 cut(s)	930
BseBI	CCWGG	4 cut(s)	437, 1610, 1619, 2248
BseCI	ATCGAT	1 cut(s)	1973
BseDI	CCNNGG	3 cut(s)	453, 2736, 3232
BseJI	GATNNNNATC	2 cut(s)	1401, 2336
BseLI	CCNNNNNNNGG	4 cut(s)	868, 2764, 2783, 2957
BseMI	GCAATG	3 cut(s)	490, 675, 1946
BseMII	CTCAG	8 cut(s)	96, 555, 900, 1010, 1095, 2141, 3002, 3327
BseNI	ACTGG	7 cut(s)	491, 1189, 2665, 2777, 3206, 3506, 3514
BsePI	GCGCGC	1 cut(s)	3238
BseRI	GAGGAG	2 cut(s)	662, 3167
BseSI	GKGCMC	2 cut(s)	2089, 2672
BsgI	GTGCAG	3 cut(s)	229, 447, 2974
Bsh1236I	CGCG	2 cut(s)	1377, 3240
BshFI	GGCC	5 cut(s)	32, 435, 2768, 2776, 2895
BshNI	GGYRCC	1 cut(s)	904
BshVI	ATCGAT	1 cut(s)	1973
BsiHKAI	GWGCWC	6 cut(s)	129, 154, 654, 2089, 2672, 2932
BsiHKCI	CYCGRG	1 cut(s)	3233
BsiSI	CCGG	8 cut(s)	147, 303, 931, 2738, 2765, 2777, 2896, 2955
BslI	CCNNNNNNNGG	4 cut(s)	868, 2764, 2783, 2957
BsmAI	GTCTC	9 cut(s)	166, 730, 802, 1247, 1785, 1871, 2033, 2269, 3510
BsmBI	CGTCTC	2 cut(s)	1247, 1785
BsmI	GAATGC	1 cut(s)	2215
BsnI	GGCC	5 cut(s)	32, 435, 2768, 2776, 2895
Bso31I	GGTCTC	2 cut(s)	730, 3510
BsoBI	CYCGRG	1 cut(s)	3233
Bsp119I	TTCGAA	1 cut(s)	829
Bsp1286I	GDGCHC	6 cut(s)	129, 154, 654, 2089, 2672, 2932
Bsp13I	TCCGGA	1 cut(s)	930
Bsp1407I	TGTACA	1 cut(s)	3350
Bsp1720I	GCTNAGC	1 cut(s)	498
BspACI	CCGC	5 cut(s)	1179, 1360, 1951, 1986, 2493
BspANI	GGCC	5 cut(s)	32, 435, 2768, 2776, 2895
BspCNI	CTCAG	8 cut(s)	97, 556, 901, 1011, 1094, 2142, 3003, 3328
BspDI	ATCGAT	1 cut(s)	1973
BspEI	TCCGGA	1 cut(s)	930
BspFNI	CGCG	2 cut(s)	1377, 3240
BspHI	TCATGA	2 cut(s)	315, 3004
BspLI	GGNNCC	9 cut(s)	368, 822, 906, 1187, 1364, 1841, 2395, 2584, 3081
BspMAI	CTGCAG	3 cut(s)	2554, 3321, 3523
BspMI	ACCTGC	1 cut(s)	1787
BspPI	GGATC	8 cut(s)	1180, 1193, 1357, 1370, 1409, 1561, 2345, 3202
BspQI	GCTCTTC	4 cut(s)	676, 1712, 2418, 3249
BspT104I	TTCGAA	1 cut(s)	829
BspT107I	GGYRCC	1 cut(s)	904
BspTNI	GGTCTC	2 cut(s)	730, 3510
BsrDI	GCAATG	3 cut(s)	490, 675, 1946
BsrFI	RCCGGY	2 cut(s)	2764, 2776
BsrGI	TGTACA	1 cut(s)	3350
BsrI	ACTGG	7 cut(s)	491, 1189, 2665, 2777, 3206, 3506, 3514
BssAI	RCCGGY	2 cut(s)	2764, 2776
BssECI	CCNNGG	3 cut(s)	453, 2736, 3232
BssHII	GCGCGC	1 cut(s)	3238
BssNAI	GTATAC	1 cut(s)	1202
BssT1I	CCWWGG	1 cut(s)	453
Bst1107I	GTATAC	1 cut(s)	1202
Bst2UI	CCWGG	4 cut(s)	437, 1610, 1619, 2248
Bst6I	CTCTTC	6 cut(s)	676, 1712, 1770, 2418, 3249, 3455
BstAPI	GCANNNNNTGC	1 cut(s)	1442
BstAUI	TGTACA	1 cut(s)	3350
BstBI	TTCGAA	1 cut(s)	829
BstC8I	GCNNGC	6 cut(s)	212, 748, 1375, 1530, 3240, 3473
BstEII	GGTNACC	2 cut(s)	1304, 1754
BstFNI	CGCG	2 cut(s)	1377, 3240
BstH2I	RGCGCY	1 cut(s)	2431
BstHHI	GCGC	4 cut(s)	1379, 2430, 3240, 3242
BstMAI	GTCTC	9 cut(s)	166, 730, 802, 1247, 1785, 1871, 2033, 2269, 3510
BstNI	CCWGG	4 cut(s)	437, 1610, 1619, 2248
BstNSI	RCATGY	3 cut(s)	750, 776, 2719
BstPI	GGTNACC	2 cut(s)	1304, 1754
BstSCI	CCNGG	5 cut(s)	435, 1608, 1617, 2246, 2736
BstSFI	CTRYAG	6 cut(s)	2297, 2550, 2673, 2994, 3317, 3519
BstSLI	GKGCMC	2 cut(s)	2089, 2672
BstUI	CGCG	2 cut(s)	1377, 3240
BstV2I	GAAGAC	2 cut(s)	2993, 3329
BstX2I	RGATCY	5 cut(s)	1185, 1362, 1566, 2009, 2161
BstXI	CCANNNNNNTGG	1 cut(s)	2275
BstYI	RGATCY	5 cut(s)	1185, 1362, 1566, 2009, 2161
BstZ17I	GTATAC	1 cut(s)	1202
Bsu15I	ATCGAT	1 cut(s)	1973
BsuI	GTATCC	2 cut(s)	2118, 3369
BsuRI	GGCC	5 cut(s)	32, 435, 2768, 2776, 2895
BsuTUI	ATCGAT	1 cut(s)	1973
BtgZI	GCGATG	1 cut(s)	1279
BtsI	GCAGTG	3 cut(s)	200, 2316, 2911
BtsIMutI	CAGTG	8 cut(s)	27, 200, 1429, 2316, 2536, 2672, 2911, 3060
BveI	ACCTGC	1 cut(s)	1787
Cac8I	GCNNGC	6 cut(s)	212, 748, 1375, 1530, 3240, 3473
CciI	TCATGA	2 cut(s)	315, 3004
CfoI	GCGC	4 cut(s)	1379, 2430, 3240, 3242
Cfr10I	RCCGGY	2 cut(s)	2764, 2776
Cfr13I	GGNCC	5 cut(s)	71, 820, 1282, 1621, 3079
ClaI	ATCGAT	1 cut(s)	1973
CseI	GACGC	3 cut(s)	379, 1264, 1802
Csp6I	GTAC	6 cut(s)	199, 693, 1496, 1846, 3351, 3499
CviQI	GTAC	6 cut(s)	199, 693, 1496, 1846, 3351, 3499
DrdI	GACNNNNNNGTC	1 cut(s)	2047
DseDI	GACNNNNNNGTC	1 cut(s)	2047
EaeI	YGGCCR	3 cut(s)	2766, 2774, 2893
Eam1104I	CTCTTC	6 cut(s)	676, 1712, 1770, 2418, 3249, 3455
EarI	CTCTTC	6 cut(s)	676, 1712, 1770, 2418, 3249, 3455
Eco130I	CCWWGG	1 cut(s)	453
Eco31I	GGTCTC	2 cut(s)	730, 3510
Eco32I	GATATC	1 cut(s)	1247
Eco47I	GGWCC	5 cut(s)	71, 820, 1282, 1621, 3079
Eco47III	AGCGCT	1 cut(s)	2429
Eco57I	CTGAAG	1 cut(s)	187
Eco88I	CYCGRG	1 cut(s)	3233
Eco91I	GGTNACC	2 cut(s)	1304, 1754
EcoO109I	RGGNCCY	3 cut(s)	820, 1282, 1621
EcoO65I	GGTNACC	2 cut(s)	1304, 1754
EcoRI	GAATTC	1 cut(s)	1613
EcoRII	CCWGG	4 cut(s)	435, 1608, 1617, 2246
EcoRV	GATATC	1 cut(s)	1247
EcoT14I	CCWWGG	1 cut(s)	453
EcoT22I	ATGCAT	1 cut(s)	604
ErhI	CCWWGG	1 cut(s)	453
Esp3I	CGTCTC	2 cut(s)	1247, 1785
FbaI	TGATCA	2 cut(s)	2526, 2646
FblI	GTMKAC	2 cut(s)	1201, 2915
FspBI	CTAG	9 cut(s)	387, 558, 572, 1100, 1274, 1704, 1772, 2375, 2469
GlaI	GCGC	4 cut(s)	1378, 2429, 3239, 3241
GsuI	CTGGAG	3 cut(s)	474, 3223, 3497
HaeII	RGCGCY	1 cut(s)	2431
HaeIII	GGCC	5 cut(s)	32, 435, 2768, 2776, 2895
HapII	CCGG	8 cut(s)	147, 303, 931, 2738, 2765, 2777, 2896, 2955
HgaI	GACGC	3 cut(s)	379, 1264, 1802
HhaI	GCGC	4 cut(s)	1379, 2430, 3240, 3242
Hin6I	GCGC	4 cut(s)	1377, 2428, 3238, 3240
HinP1I	GCGC	4 cut(s)	1377, 2428, 3238, 3240
HincII	GTYRAC	2 cut(s)	294, 711
HindII	GTYRAC	2 cut(s)	294, 711
HindIII	AAGCTT	3 cut(s)	500, 524, 3345
HinfI	GANTC	9 cut(s)	176, 1120, 1336, 1888, 1980, 2105, 2381, 3173, 3524
HpaII	CCGG	8 cut(s)	147, 303, 931, 2738, 2765, 2777, 2896, 2955
HphI	GGTGA	8 cut(s)	22, 472, 1061, 1082, 1316, 1345, 1766, 2441
Hpy166II	GTNNAC	9 cut(s)	294, 577, 711, 1202, 1966, 2087, 2670, 2762, 2916
Hpy8I	GTNNAC	9 cut(s)	294, 577, 711, 1202, 1966, 2087, 2670, 2762, 2916
HspAI	GCGC	4 cut(s)	1377, 2428, 3238, 3240
Kpn2I	TCCGGA	1 cut(s)	930
Ksp22I	TGATCA	2 cut(s)	2526, 2646
LguI	GCTCTTC	4 cut(s)	676, 1712, 2418, 3249
LmnI	GCTCC	7 cut(s)	149, 493, 649, 1585, 2198, 2393, 3204
LweI	GCATC	7 cut(s)	325, 1256, 1381, 1423, 1600, 2543, 3296
MaeI	CTAG	9 cut(s)	387, 558, 572, 1100, 1274, 1704, 1772, 2375, 2469
MaeIII	GTNAC	9 cut(s)	22, 183, 325, 768, 1304, 1504, 1672, 1754, 2242
MfeI	CAATTG	3 cut(s)	266, 1760, 1854
MflI	RGATCY	5 cut(s)	1185, 1362, 1566, 2009, 2161
MhlI	GDGCHC	6 cut(s)	129, 154, 654, 2089, 2672, 2932
MlyI	GAGTC	4 cut(s)	1897, 2099, 2390, 3167
MmeI	TCCRAC	2 cut(s)	1390, 2536
Mph1103I	ATGCAT	1 cut(s)	604
MroI	TCCGGA	1 cut(s)	930
MroXI	GAANNNNTTC	1 cut(s)	1212
MslI	CAYNNNNRTG	2 cut(s)	303, 2273
MspA1I	CMGCKG	1 cut(s)	1360
MspI	CCGG	8 cut(s)	147, 303, 931, 2738, 2765, 2777, 2896, 2955
MspR9I	CCNGG	5 cut(s)	437, 1610, 1619, 2248, 2738
MunI	CAATTG	3 cut(s)	266, 1760, 1854
Mva1269I	GAATGC	1 cut(s)	2215
MvaI	CCWGG	4 cut(s)	437, 1610, 1619, 2248
MvnI	CGCG	2 cut(s)	1377, 3240
NciI	CCSGG	1 cut(s)	2738
NlaIV	GGNNCC	9 cut(s)	368, 822, 906, 1187, 1364, 1841, 2395, 2584, 3081
NmeAIII	GCCGAG	2 cut(s)	2404, 2414
NmuCI	GTSAC	6 cut(s)	22, 183, 1304, 1504, 1672, 1754
NsiI	ATGCAT	1 cut(s)	604
NspI	RCATGY	3 cut(s)	750, 776, 2719
NspV	TTCGAA	1 cut(s)	829
OliI	CACNNNNGTG	1 cut(s)	303
PaeI	GCATGC	1 cut(s)	750
PagI	TCATGA	2 cut(s)	315, 3004
PauI	GCGCGC	1 cut(s)	3238
PciSI	GCTCTTC	4 cut(s)	676, 1712, 2418, 3249
PctI	GAATGC	1 cut(s)	2215
PdmI	GAANNNNTTC	1 cut(s)	1212
PfeI	GAWTC	5 cut(s)	176, 1120, 1336, 1980, 3524
PflFI	GACNNNGTC	1 cut(s)	1625
PfoI	TCCNGGA	1 cut(s)	1617
PleI	GAGTC	4 cut(s)	1896, 2099, 2389, 3167
PpsI	GAGTC	4 cut(s)	1896, 2099, 2389, 3167
PpuMI	RGGWCCY	3 cut(s)	820, 1282, 1621
Psp5II	RGGWCCY	3 cut(s)	820, 1282, 1621
Psp6I	CCWGG	4 cut(s)	435, 1608, 1617, 2246
PspEI	GGTNACC	2 cut(s)	1304, 1754
PspGI	CCWGG	4 cut(s)	435, 1608, 1617, 2246
PspN4I	GGNNCC	9 cut(s)	368, 822, 906, 1187, 1364, 1841, 2395, 2584, 3081
PspPI	GGNCC	5 cut(s)	71, 820, 1282, 1621, 3079
PspPPI	RGGWCCY	3 cut(s)	820, 1282, 1621
PstI	CTGCAG	3 cut(s)	2554, 3321, 3523
PsuI	RGATCY	5 cut(s)	1185, 1362, 1566, 2009, 2161
PsyI	GACNNNGTC	1 cut(s)	1625
PteI	GCGCGC	1 cut(s)	3238
RsaI	GTAC	6 cut(s)	200, 694, 1497, 1847, 3352, 3500
RsaNI	GTAC	6 cut(s)	199, 693, 1496, 1846, 3351, 3499
RseI	CAYNNNNRTG	2 cut(s)	303, 2273
SapI	GCTCTTC	4 cut(s)	676, 1712, 2418, 3249
Sau96I	GGNCC	5 cut(s)	71, 820, 1282, 1621, 3079
ScaI	AGTACT	3 cut(s)	694, 1497, 3500
SchI	GAGTC	4 cut(s)	1897, 2099, 2390, 3167
ScrFI	CCNGG	5 cut(s)	437, 1610, 1619, 2248, 2738
SduI	GDGCHC	6 cut(s)	129, 154, 654, 2089, 2672, 2932
SfaNI	GCATC	7 cut(s)	325, 1256, 1381, 1423, 1600, 2543, 3296
SfcI	CTRYAG	6 cut(s)	2297, 2550, 2673, 2994, 3317, 3519
SfuI	TTCGAA	1 cut(s)	829
SinI	GGWCC	5 cut(s)	71, 820, 1282, 1621, 3079
SmiMI	CAYNNNNRTG	2 cut(s)	303, 2273
SmlI	CTYRAG	6 cut(s)	551, 899, 1259, 1286, 2036, 3036
SmoI	CTYRAG	6 cut(s)	551, 899, 1259, 1286, 2036, 3036
SpeI	ACTAGT	1 cut(s)	1273
SphI	GCATGC	1 cut(s)	750
SsiI	CCGC	5 cut(s)	1179, 1360, 1951, 1986, 2493
SspI	AATATT	3 cut(s)	1141, 1213, 2306
SspMI	CTAG	9 cut(s)	387, 558, 572, 1100, 1274, 1704, 1772, 2375, 2469
StyD4I	CCNGG	5 cut(s)	435, 1608, 1617, 2246, 2736
StyI	CCWWGG	1 cut(s)	453
TatI	WGTACW	5 cut(s)	692, 1495, 1845, 3350, 3498
TfiI	GAWTC	5 cut(s)	176, 1120, 1336, 1980, 3524
TscAI	CASTG	8 cut(s)	27, 200, 1429, 2323, 2536, 2672, 2911, 3067
TseFI	GTSAC	6 cut(s)	22, 183, 1304, 1504, 1672, 1754
Tsp45I	GTSAC	6 cut(s)	22, 183, 1304, 1504, 1672, 1754
TspGWI	ACGGA	2 cut(s)	413, 756
TspRI	CASTG	8 cut(s)	27, 200, 1429, 2323, 2536, 2672, 2911, 3067
Tth111I	GACNNNGTC	1 cut(s)	1625
VneI	GTGCAC	2 cut(s)	2085, 2668
VpaK11BI	GGWCC	5 cut(s)	71, 820, 1282, 1621, 3079
XapI	RAATTY	7 cut(s)	762, 893, 1000, 1030, 1613, 1687, 2803
XbaI	TCTAGA	1 cut(s)	1703
XceI	RCATGY	3 cut(s)	750, 776, 2719
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2769
XmiI	GTMKAC	2 cut(s)	1201, 2915
XmnI	GAANNNNTTC	1 cut(s)	1212
XspI	CTAG	9 cut(s)	387, 558, 572, 1100, 1274, 1704, 1772, 2375, 2469
ZrmI	AGTACT	3 cut(s)	694, 1497, 3500
Zsp2I	ATGCAT	1 cut(s)	604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.