Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0002769

Physical Location & Seq

Genomic Coordinates Reverse (-)

65813970 .. 65820824

Gene Structure 6855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G038220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4452 bp

ATGAAAAGAGTAGGCTCACTATTCGGGCAGCTTGATTTATGGACTGGATTTTGTGGGGAATCTGAAGATTCTGAGAGTTTGAGTTTACGGTTTCTGATTCATCCTACTTCATGCATTAATCACGCATTGACCATTTTCCTTGACATAGTTCTTCTTGTGGTGCTCTTGTTCAGTATGTTTATCAAGTCATCAAAAAGAGTTCATGTTCCAGCACGATTTCTAGGCTTTTCGAGTTGGCAAATAGTCTCGGCCATTTTCAATGGCGCTCTTGGATTTGTGTACTTGTGCTTCGGAGCCTGGAATTTAGAAGAGGAGCTGAGGAATACACGTACTGCTTTGCCTTTGAATTGGTGGTTACTTGGTCTCTTTCAAGGCCTCACATGGTTGCTTTTGAGCTTAACTCTAAGCATCAGGGCGAAGCAACTGCCAAGACAACCTTCAAGAGTGATGTGCATCCTGGCTTTTTTGTTTTCTGGAGTTGTCTTTGCTCTATCGCTCTTCACAGTCATTTTCAGAAATGAAATGTCGGTTAAGATAGTACTAGATATCCTAACTTTTCCAGGAGCAGCATTGTTGCTGCTATGTGTTTTCAAGGGGTATAAGTATGAAGATGGTGATGAGATCATCAGTGACGATGGCCTTTATGCCCCTCTCAATGGTGACTCCAATGGCATTAGTAAAGGTCATGATCATGCAACCCCATTTAGCAAAGCTGGATTGTTAAGTAAAATGTCATTTTGGTGGTTAAATTCATTGATGAAAAGAGGTAGGGAGAAAACTCTGGAGGATGAAGATATACCGAAGTTGCGCAAGGAAGATAGAGCAGAAAATTGCTATGCGATGTTCTTGGAGCAACTAAGCAAGCAGAAACAAATTGAGCCATCTTCCCAGCCATCAGTATTAAAGACTATAATCCTATGCCACCGGAAGGAGATTTTACTATCTGGTTTCTTTGCTTTGCTGAAGATACTCACTATCTGTGCTGGTCCTTTGCTTCTCAATGCCTTCATTTTGGTTGCAGAGGGAAATGAAAGTTTCGAGCATGAAGGTTATCTGTTAGCCGGAGCACTTTTTGTTTCGAAAACCATTGAATCCTTGTCACAAAGGCAGTGGTACTTCAGGTGCAGGCTTATTGGTTTGAAAGTTAGGTCTTTGCTGACAGCAGCCATTTACAAAAAGCAATTAAGATTATCTAATGCTGCTAAGTTGACACACTCAGGTGGTGAGATCATGAATTATGTTACTGTGGATGCTTATAGGATTGGAGAGTTTCCATTTTGGTTCCATAAGACTTGGACTACTAGCGTCCAGCTCTGTTTTTCTTTGGTAATTTTTTTCCGTGCAGTTGGCCTGGCTACATTTGCAGCCTTGGTGGTGATACTTTTGGGTGTGATTTGCAATGCTCCAGTTGCTAAGCTACAACATAAGTTCCAGAGTAAGCTTATGAAGGCTCAAGATGAAAGGCTCAAGGCTAGTTCTGAGGCTCTAGTGAACATGAAGGTCTTAAAGTTATATGCATGGGAAACACATTTCAAGAAGGCAATAGAAAAGTTAAGGAAGGAGGAACACAAATGGTTATCTGCAATGCTGTTAAGAAGAGCATATAGTACTTATCTCTTTTGGTCAACTCCTGTTTTGGTCTCTACTGCAACATTTGGGGCATGCTATTTTCTCAAAATTCCGTTACATGCAAACAATGTTTTCACTTTTATCTCTACTTTGAGACTTGTTCAGGACCCCATTCAAACCATACCTGATGTTATTGCGGTTGTCATTCAAGCCAAGGTTGCATTTGGTCGTATTGTGAAATTCCTTGAGGCACCTGAGCTGCAAACTTCAAATGTCCGGAAGTGCAACATGAAGAATGTAGCTAACTCCATTGAAATTAAGTCAGCTAATTTTTCATGGGAAGAGAATTTATCGAAGTCCATACTGAGAAATATAAATTTAGAGGTTAGACCTGGTGAAAAGGTGGCTATATGTGGTGAAGTCGGCTCAGGCAAGTCAAGCCTTGTAGCTGCAATTCTTGGAGAAATTCCAAATGTGCAAGGAAATATTCAAGTTTATGGGAAGATTGCCTATGTTTCTCAAACAGCATGGATCCAGTCAGGAACTATACAAGAAAATATTCTCTTTGGTTCTTGTATGGATAGTCAGAGATATCGAGAGACGCTTGAGAGATGCTCACTAGTAAAGGACCTTGAGCTGCTTCCCTACGGTGACCTTACTGAAATAGGAGAGAGAGGGGTGAATCTAAGTGGAGGTCAGAAGCAGCGGATCCAACTTGCACGCGCTCTCTATCAGGATGCTGATATATATCTATTGGATGATCCATTCAGTGCTGTTGATGCACATACTGCCACAAACTTATTTAATGAATATGTAATGGAAGCACTTTCAGGTAAGACAGTACTACTTGTGACCCATCAAGTTGATTTCCTGCCTGCATTTGATTTCGTTTTGTTGATGATGGATGGGGAGATCCTACAAGCAGCTCCTTATCAGCATCTATTGGCTTTAAGCCAAGAATTCCAGAACCTTGTCAATGCACACAAAGAAACTGCTGGTTCCGAAAGGCTTTCAGATGTCACTTCTGCTCAAAAATCTGGAATATCATCTAGAGAGATTAAGAAGAGTTATGTTGAGAAGCCATTAAAAGAAAATAAAGTTGACCAATTGATTAAACTTGAAGAGAGAGAAACAGGTGACACAGGTTTTAAGCCATACATACTGTATCTGAAGCAGAACAAAGGGTTCCTGTACTTCTCAATTGCCGTTTGTCTACACTTCACTTCCATTATGAGTCAGATTGCACAGAACTCTTGGATGGCTGCTAATGTTGATAATCCGAGTATTAGCACATTGCGGTTGCTTGTGGTTTACTTATCGATTGGATTCTCCGCAACATTTATTTTTCTGTTTAGATCTCTTGCAGCAGTTGTCGGGGGCCTTGAGACATCAAAGTCTTTGTTCTCACAACTACTAAACTCCCTTTTTCGTGCACCCATGTCATTTATGACTCAACACCACTGGGAAGGATACTTAGTCGGGTATGTTTCATCTGATCTGAGTATCACAGATCTTGATATTCCATTCAGCATAGTATTTGCTTGTGGAGTTACCATGAATGCCTATTGCAATCTTGGGGTATTGACTGTTGTTACCTGGCAAGTCCTGTTTGTCTCCATCCCAATGGTCTGTGTCGCAATTCAGCTACAGAAGTACTATTTTTCTACTGCAAAAGCGTTGATGAGGATCAATGGAACAACTAAGTCCTTTGTAGCAAACCATCTCGCAGAGTCTGTCTCAGGAGCCATTACCATAAGAGCTTTCAATGAAGAAGAGCGCTTCTCGGCAAAGAACTTTCACCTCATTGACACAAATGCTAGTCCTTATTTCCACAATTTTTCTGCAAATGAGTGGTTAATTCAACGATTAGGGATGATCAGCGCGGTGGTTCTTGCCTCTGCAGCACTTTGTATGGTTTTGCTTCCTCATGGAACTTTTACCTCTGGGTTTATTGGGATGGCACTTTCTTATGGTCTATCATTAAATGTGTCCTTGATCGACTCAATTCAGAACCAGTGCACTATAGCAAATTATATCATTTCTGTTGAAAGACTAAATCAATACATGCACATTCCTAGTGAAGCCCCGGAAGTAATGGAGGGAAACCGTCCGCCAGCCAACTGGCCTGTTGTAGGAAAAGTGGAGATACAAAATTTGCGGATGAGATATAGGGATGACACACCACTTGTACTTCGAGGGATTAGTTGTGTTTTTGAAGGAGGACATAAGATTGGTATTGTTGGCCGGACAGGCAGTGGGAAGTCTACACTCATCAGCGCTCTATTTCGGCTGGTCGAGCCAACCGGAGGGAAGGTCATAGTCGATGGCATTGACATCTCTACTATAGGACTTCATGATCTGAGGTCAAGATTCGGAATAATACCTCAAGACCCTACTCTCTTTAATGGCACCGTCAGATACAATTTGGACCCCTTATCTCAACATTCAGACCAAGAAATATGGGAGGTTCTTGGGAAGTGTCAGCTTCGAGAGGCTGTTGAGGAGAAAGAAAAAGGCTTGGACTCGTTAGTTGTGCATGATGGATCAAACTGGAGCATGGGGCAAAGGCAACTGTTCTGCCTCGGGCGCGCTCTTCTAAGGAGAAGTCGGGTATTGGTGCTTGATGAAGCAACTGCATCCATCGACAATGCAACTGATACGATCCTGCAGAAGACTATAAGGACTGAGTTTGAATCTTGTACAGTAATTACAGTAGCCCACAGGATACCTACTGTGATGGATTGCACAATGGTTCTTGCCATTAGTGATGGAAAGATAGTGGAGTATGATGAGCCAATGCGGTTGATGAAGAGAGAAGGCTCGCTGTTTGGGCAGCTTGTCAAGGAGTACTGGTCTCATCTCCAGTCTGCAGAATCTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0008150 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04976 map01523 map01524 map02010 map04976

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1483

Amino Acids

166.03

Weight (kDa)

8.26

Isoelectric Point (pI)

34.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABCC10_N	PF24358	12 - 202	4.2e-74	ABCC10 N-terminal domain
ABC_membrane	PF00664	313 - 580	4.3e-24	ABC transporter transmembrane region
ABC_tran	PF00005	645 - 779	2.3e-22	ABC transporter
ABC_membrane	PF00664	920 - 1180	7.7e-17	ABC transporter transmembrane region
ABC_tran	PF00005	1254 - 1402	2.1e-25	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002769)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59140
fragaria_vesca	FvH4_3g44221 FvH4_3g44230 FvH4_3g44230 FvH4_7g30860 FvH4_7g30860 FvH4_7g30860 FvH4_7g30870 FvH4_7g30870 FvH4_7g30870 FvH4_7g30870 FvH4_7g30870
malus_domestica	MD01G1213600.v1.1
prunus_persica	Prupe.2G305800_v2.0.a1 Prupe.2G305800_v2.0.a1
pyrus_communis	pycom01g22440 pycom07g25750
rosa_chinensis	RchiOBHm_Chr1g0379791 RchiOBHm_Chr1g0379831 RchiOBHm_Chr1g0379841
rosa_laevigata	RLG00000026355 RLG00000026356 RLG00000026359 RLG00000026360
rosa_multiflora	Rmu_sc0014259.1_g000008 Rmu_sc0014259.1_g000011
rosa_roxburghii	Rroxscaffold_4G00279900 Rroxscaffold_4G00279910
rosa_rugosa	Rorug01G0415300
rosa_samantha	Rh1AG438800 Rh1AG439300 Rh1AG439400 Rh1BG395300 Rh1BG395400 Rh1DG426200 Rh1DG426300
rosa_wichuraiana	Rw1G038220 Rw1G038240 Rw1G038250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2962
Acc16I	TGCGCA	1 cut(s)	809
AccB1I	GGYRCC	2 cut(s)	1819, 3977
AccI	GTMKAC	2 cut(s)	2782, 3833
AccII	CGCG	3 cut(s)	2292, 3452, 4158
AccIII	TCCGGA	1 cut(s)	1845
AciI	CCGC	8 cut(s)	1766, 2275, 2866, 2901, 3452, 3680, 3727, 4369
AclWI	GGATC	9 cut(s)	2095, 2108, 2272, 2285, 2324, 2476, 3263, 4120, 4225
AcoI	YGGCCR	2 cut(s)	249, 3811
AcsI	RAATTY	9 cut(s)	301, 748, 1677, 1808, 1915, 1945, 2034, 2528, 3721
AcuI	CTGAAG	4 cut(s)	84, 983, 1102, 2759
AfeI	AGCGCT	2 cut(s)	3347, 3847
AfiI	CCNNNNNNNGG	4 cut(s)	656, 928, 3701, 3875
AflIII	ACRYGT	1 cut(s)	326
AhlI	ACTAGT	1 cut(s)	2188
AjnI	CCWGG	6 cut(s)	296, 456, 559, 1350, 1960, 3164
AjuI	GAANNNNNNNTTGG	2 cut(s)	421, 453
AleI	CACNNNNGTG	1 cut(s)	1218
Alw21I	GWGCWC	4 cut(s)	165, 1069, 3004, 3590
Alw26I	GTCTC	9 cut(s)	250, 368, 1645, 1717, 2162, 2948, 3187, 3310, 4428
Alw44I	GTGCAC	2 cut(s)	3000, 3586
AlwI	GGATC	9 cut(s)	2095, 2108, 2272, 2285, 2324, 2476, 3263, 4120, 4225
AlwNI	CAGNNNCTG	1 cut(s)	3302
Ama87I	CYCGRG	1 cut(s)	4151
Aor13HI	TCCGGA	1 cut(s)	1845
Aor51HI	AGCGCT	2 cut(s)	3347, 3847
AoxI	GGCC	7 cut(s)	249, 373, 637, 1348, 2947, 3692, 3811
ApaLI	GTGCAC	2 cut(s)	3000, 3586
ApoI	RAATTY	9 cut(s)	301, 748, 1677, 1808, 1915, 1945, 2034, 2528, 3721
AseI	ATTAAT	1 cut(s)	117
Asp700I	GAANNNNTTC	1 cut(s)	2127
AspLEI	GCGC	8 cut(s)	266, 810, 2294, 3348, 3452, 3848, 4158, 4160
AspS9I	GGNCC	5 cut(s)	986, 1735, 2197, 2947, 3997
AsuC2I	CCSGG	1 cut(s)	3656
AsuII	TTCGAA	1 cut(s)	1079
AvaI	CYCGRG	1 cut(s)	4151
AvaII	GGWCC	4 cut(s)	986, 1735, 2197, 3997
BaeGI	GKGCMC	2 cut(s)	3004, 3590
BamHI	GGATCC	2 cut(s)	2100, 2277
BanI	GGYRCC	2 cut(s)	1819, 3977
BarI	GAAGNNNNNNTAC	2 cut(s)	272, 304
BbsI	GAAGAC	1 cut(s)	4247
Bbv12I	GWGCWC	4 cut(s)	165, 1069, 3004, 3590
BbvCI	CCTCAGC	1 cut(s)	317
BceAI	ACGGC	1 cut(s)	2759
BcgI	CGANNNNNNTGC	2 cut(s)	406, 440
BciT130I	CCWGG	6 cut(s)	298, 458, 561, 1352, 1962, 3166
BciVI	GTATCC	2 cut(s)	3032, 4287
BclI	TGATCA	2 cut(s)	688, 3444
BcnI	CCSGG	1 cut(s)	3656
BcoDI	GTCTC	9 cut(s)	250, 368, 1645, 1717, 2162, 2948, 3187, 3310, 4428
BcuI	ACTAGT	1 cut(s)	2188
BfaI	CTAG	9 cut(s)	221, 542, 1302, 1473, 1487, 2189, 2619, 3387, 3645
BfmI	CTRYAG	6 cut(s)	3215, 3468, 3591, 3912, 4235, 4437
BfoI	RGCGCY	3 cut(s)	267, 3349, 3849
BfuI	GTATCC	2 cut(s)	3032, 4287
BglI	GCCNNNNNGGC	1 cut(s)	3819
BglII	AGATCT	2 cut(s)	2924, 3079
BlpI	GCTNAGC	1 cut(s)	1413
BmcAI	AGTACT	5 cut(s)	540, 1609, 2412, 3224, 4418
Bme1390I	CCNGG	7 cut(s)	298, 458, 561, 1352, 1962, 3166, 3656
Bme18I	GGWCC	4 cut(s)	986, 1735, 2197, 3997
BmeT110I	CYCGRG	1 cut(s)	4151
BmgT120I	GGNCC	5 cut(s)	986, 1735, 2197, 2947, 3997
BmrFI	CCNGG	7 cut(s)	298, 458, 561, 1352, 1962, 3166, 3656
BmrI	ACTGGG	1 cut(s)	3040
BmsI	GCATC	8 cut(s)	417, 462, 1240, 2171, 2296, 2338, 2515, 4214
BmuI	ACTGGG	1 cut(s)	3040
BpiI	GAAGAC	1 cut(s)	4247
BplI	GAGNNNNNCTC	8 cut(s)	385, 417, 2168, 2200, 3290, 3322, 3335, 3367
BpmI	CTGGAG	5 cut(s)	495, 803, 1389, 4141, 4415
Bpu10I	CCTNAGC	3 cut(s)	317, 1824, 1996
Bpu1102I	GCTNAGC	1 cut(s)	1413
Bpu14I	TTCGAA	1 cut(s)	1079
BpuEI	CTTGAG	7 cut(s)	1437, 1451, 1835, 2195, 2222, 2972, 3939
BpuMI	CCSGG	1 cut(s)	3656
Bsa29I	ATCGAT	1 cut(s)	2888
BsaAI	YACGTR	1 cut(s)	329
BsaBI	GATNNNNATC	3 cut(s)	452, 2316, 3254
BsaI	GGTCTC	3 cut(s)	368, 1645, 4428
BsaJI	CCNNGG	4 cut(s)	1368, 1782, 3654, 4150
BsaWI	WCCGGW	3 cut(s)	924, 1845, 3872
BsaXI	ACNNNNNCTCC	4 cut(s)	3867, 3897, 4406, 4436
Bsc4I	CCNNNNNNNGG	4 cut(s)	656, 928, 3701, 3875
Bse1I	ACTGG	9 cut(s)	49, 1406, 2104, 3035, 3583, 3695, 4124, 4424, 4432
Bse3DI	GCAATG	3 cut(s)	1405, 1590, 2861
Bse8I	GATNNNNATC	3 cut(s)	452, 2316, 3254
BseAI	TCCGGA	1 cut(s)	1845
BseBI	CCWGG	6 cut(s)	298, 458, 561, 1352, 1962, 3166
BseCI	ATCGAT	1 cut(s)	2888
BseDI	CCNNGG	4 cut(s)	1368, 1782, 3654, 4150
BseJI	GATNNNNATC	3 cut(s)	452, 2316, 3254
BseLI	CCNNNNNNNGG	4 cut(s)	656, 928, 3701, 3875
BseMI	GCAATG	3 cut(s)	1405, 1590, 2861
BseNI	ACTGG	9 cut(s)	49, 1406, 2104, 3035, 3583, 3695, 4124, 4424, 4432
BsePI	GCGCGC	1 cut(s)	4156
BseRI	GAGGAG	2 cut(s)	326, 4085
BseSI	GKGCMC	2 cut(s)	3004, 3590
BseYI	CCCAGC	1 cut(s)	888
BsgI	GTGCAG	2 cut(s)	1144, 1362
Bsh1236I	CGCG	3 cut(s)	2292, 3452, 4158
BshFI	GGCC	7 cut(s)	251, 375, 639, 1350, 2949, 3694, 3813
BshNI	GGYRCC	2 cut(s)	1819, 3977
BshVI	ATCGAT	1 cut(s)	2888
BsiHKAI	GWGCWC	4 cut(s)	165, 1069, 3004, 3590
BsiHKCI	CYCGRG	1 cut(s)	4151
BsiSI	CCGG	6 cut(s)	925, 1062, 1846, 3656, 3814, 3873
BslI	CCNNNNNNNGG	4 cut(s)	656, 928, 3701, 3875
BsmAI	GTCTC	9 cut(s)	250, 368, 1645, 1717, 2162, 2948, 3187, 3310, 4428
BsmBI	CGTCTC	1 cut(s)	2162
BsmI	GAATGC	1 cut(s)	3133
BsnI	GGCC	7 cut(s)	251, 375, 639, 1350, 2949, 3694, 3813
Bso31I	GGTCTC	3 cut(s)	368, 1645, 4428
BsoBI	CYCGRG	1 cut(s)	4151
Bsp119I	TTCGAA	1 cut(s)	1079
Bsp1286I	GDGCHC	4 cut(s)	165, 1069, 3004, 3590
Bsp13I	TCCGGA	1 cut(s)	1845
Bsp1407I	TGTACA	1 cut(s)	4268
Bsp1720I	GCTNAGC	1 cut(s)	1413
BspACI	CCGC	8 cut(s)	1766, 2275, 2866, 2901, 3452, 3680, 3727, 4369
BspANI	GGCC	7 cut(s)	251, 375, 639, 1350, 2949, 3694, 3813
BspDI	ATCGAT	1 cut(s)	2888
BspEI	TCCGGA	1 cut(s)	1845
BspFNI	CGCG	3 cut(s)	2292, 3452, 4158
BspHI	TCATGA	3 cut(s)	685, 1230, 3922
BspMAI	CTGCAG	3 cut(s)	3472, 4239, 4441
BspPI	GGATC	9 cut(s)	2095, 2108, 2272, 2285, 2324, 2476, 3263, 4120, 4225
BspQI	GCTCTTC	4 cut(s)	503, 1591, 3336, 4167
BspT104I	TTCGAA	1 cut(s)	1079
BspT107I	GGYRCC	2 cut(s)	1819, 3977
BspTNI	GGTCTC	3 cut(s)	368, 1645, 4428
BsrDI	GCAATG	3 cut(s)	1405, 1590, 2861
BsrGI	TGTACA	1 cut(s)	4268
BsrI	ACTGG	9 cut(s)	49, 1406, 2104, 3035, 3583, 3695, 4124, 4424, 4432
BssECI	CCNNGG	4 cut(s)	1368, 1782, 3654, 4150
BssHII	GCGCGC	1 cut(s)	4156
BssT1I	CCWWGG	2 cut(s)	1368, 1782
Bst2UI	CCWGG	6 cut(s)	298, 458, 561, 1352, 1962, 3166
Bst6I	CTCTTC	9 cut(s)	303, 503, 1591, 1905, 2627, 2685, 3336, 4167, 4373
BstAPI	GCANNNNNTGC	1 cut(s)	2357
BstAUI	TGTACA	1 cut(s)	4268
BstBAI	YACGTR	1 cut(s)	329
BstBI	TTCGAA	1 cut(s)	1079
BstC8I	GCNNGC	8 cut(s)	863, 1127, 1663, 2290, 2445, 3684, 4158, 4391
BstEII	GGTNACC	1 cut(s)	2219
BstENI	CCTNNNNNAGG	1 cut(s)	3699
BstFNI	CGCG	3 cut(s)	2292, 3452, 4158
BstH2I	RGCGCY	3 cut(s)	267, 3349, 3849
BstHHI	GCGC	8 cut(s)	266, 810, 2294, 3348, 3452, 3848, 4158, 4160
BstMAI	GTCTC	9 cut(s)	250, 368, 1645, 1717, 2162, 2948, 3187, 3310, 4428
BstNI	CCWGG	6 cut(s)	298, 458, 561, 1352, 1962, 3166
BstNSI	RCATGY	3 cut(s)	1665, 1691, 3637
BstPI	GGTNACC	1 cut(s)	2219
BstSCI	CCNGG	7 cut(s)	296, 456, 559, 1350, 1960, 3164, 3654
BstSFI	CTRYAG	6 cut(s)	3215, 3468, 3591, 3912, 4235, 4437
BstSLI	GKGCMC	2 cut(s)	3004, 3590
BstUI	CGCG	3 cut(s)	2292, 3452, 4158
BstV2I	GAAGAC	1 cut(s)	4247
BstX2I	RGATCY	5 cut(s)	2100, 2277, 2481, 2924, 3079
BstXI	CCANNNNNNTGG	2 cut(s)	3193, 3690
BstYI	RGATCY	5 cut(s)	2100, 2277, 2481, 2924, 3079
Bsu15I	ATCGAT	1 cut(s)	2888
BsuI	GTATCC	2 cut(s)	3032, 4287
BsuRI	GGCC	7 cut(s)	251, 375, 639, 1350, 2949, 3694, 3813
BsuTUI	ATCGAT	1 cut(s)	2888
BtgZI	GCGATG	1 cut(s)	854
BtsI	GCAGTG	2 cut(s)	1115, 3829
BtsIMutI	CAGTG	6 cut(s)	634, 1115, 2344, 3028, 3590, 3829
Cac8I	GCNNGC	8 cut(s)	863, 1127, 1663, 2290, 2445, 3684, 4158, 4391
CaiI	CAGNNNCTG	1 cut(s)	3302
CciI	TCATGA	3 cut(s)	685, 1230, 3922
CfoI	GCGC	8 cut(s)	266, 810, 2294, 3348, 3452, 3848, 4158, 4160
Cfr13I	GGNCC	5 cut(s)	986, 1735, 2197, 2947, 3997
ClaI	ATCGAT	1 cut(s)	2888
CseI	GACGC	2 cut(s)	1294, 2179
CsiI	ACCWGGT	1 cut(s)	1960
CspCI	CAANNNNNGTGG	2 cut(s)	1091, 1126
DrdI	GACNNNNNNGTC	1 cut(s)	2962
DseDI	GACNNNNNNGTC	1 cut(s)	2962
EaeI	YGGCCR	2 cut(s)	249, 3811
Eam1104I	CTCTTC	9 cut(s)	303, 503, 1591, 1905, 2627, 2685, 3336, 4167, 4373
EarI	CTCTTC	9 cut(s)	303, 503, 1591, 1905, 2627, 2685, 3336, 4167, 4373
EciI	GGCGGA	1 cut(s)	3669
Eco130I	CCWWGG	2 cut(s)	1368, 1782
Eco147I	AGGCCT	1 cut(s)	375
Eco31I	GGTCTC	3 cut(s)	368, 1645, 4428
Eco32I	GATATC	2 cut(s)	547, 2162
Eco47I	GGWCC	4 cut(s)	986, 1735, 2197, 3997
Eco47III	AGCGCT	2 cut(s)	3347, 3847
Eco57I	CTGAAG	4 cut(s)	84, 983, 1102, 2759
Eco88I	CYCGRG	1 cut(s)	4151
Eco91I	GGTNACC	1 cut(s)	2219
EcoNI	CCTNNNNNAGG	1 cut(s)	3699
EcoO109I	RGGNCCY	3 cut(s)	1735, 2197, 2947
EcoO65I	GGTNACC	1 cut(s)	2219
EcoRI	GAATTC	1 cut(s)	2528
EcoRII	CCWGG	6 cut(s)	296, 456, 559, 1350, 1960, 3164
EcoRV	GATATC	2 cut(s)	547, 2162
EcoT14I	CCWWGG	2 cut(s)	1368, 1782
EcoT22I	ATGCAT	2 cut(s)	116, 1519
ErhI	CCWWGG	2 cut(s)	1368, 1782
Esp3I	CGTCTC	1 cut(s)	2162
FalI	AAGNNNNNCTT	2 cut(s)	421, 453
FbaI	TGATCA	2 cut(s)	688, 3444
FblI	GTMKAC	2 cut(s)	2782, 3833
FspBI	CTAG	9 cut(s)	221, 542, 1302, 1473, 1487, 2189, 2619, 3387, 3645
FspI	TGCGCA	1 cut(s)	809
GlaI	GCGC	8 cut(s)	265, 809, 2293, 3347, 3451, 3847, 4157, 4159
GsaI	CCCAGC	1 cut(s)	892
GsuI	CTGGAG	5 cut(s)	495, 803, 1389, 4141, 4415
HaeII	RGCGCY	3 cut(s)	267, 3349, 3849
HaeIII	GGCC	7 cut(s)	251, 375, 639, 1350, 2949, 3694, 3813
HapII	CCGG	6 cut(s)	925, 1062, 1846, 3656, 3814, 3873
HgaI	GACGC	2 cut(s)	1294, 2179
HhaI	GCGC	8 cut(s)	266, 810, 2294, 3348, 3452, 3848, 4158, 4160
Hin6I	GCGC	8 cut(s)	264, 808, 2292, 3346, 3450, 3846, 4156, 4158
HinP1I	GCGC	8 cut(s)	264, 808, 2292, 3346, 3450, 3846, 4156, 4158
HincII	GTYRAC	3 cut(s)	1209, 1626, 2671
HindII	GTYRAC	3 cut(s)	1209, 1626, 2671
HindIII	AAGCTT	1 cut(s)	1439
HpaII	CCGG	6 cut(s)	925, 1062, 1846, 3656, 3814, 3873
HpyCH4IV	ACGT	1 cut(s)	328
HpySE526I	ACGT	1 cut(s)	328
HspAI	GCGC	8 cut(s)	264, 808, 2292, 3346, 3450, 3846, 4156, 4158
Kpn2I	TCCGGA	1 cut(s)	1845
Ksp22I	TGATCA	2 cut(s)	688, 3444
LguI	GCTCTTC	4 cut(s)	503, 1591, 3336, 4167
LmnI	GCTCC	9 cut(s)	293, 313, 563, 850, 1064, 1408, 2500, 3311, 4122
LweI	GCATC	8 cut(s)	417, 462, 1240, 2171, 2296, 2338, 2515, 4214
MabI	ACCWGGT	1 cut(s)	1960
MaeI	CTAG	9 cut(s)	221, 542, 1302, 1473, 1487, 2189, 2619, 3387, 3645
MaeII	ACGT	1 cut(s)	328
MfeI	CAATTG	2 cut(s)	2675, 2769
MflI	RGATCY	5 cut(s)	2100, 2277, 2481, 2924, 3079
MhlI	GDGCHC	4 cut(s)	165, 1069, 3004, 3590
MlyI	GAGTC	6 cut(s)	656, 2812, 3013, 3308, 3563, 4085
MmeI	TCCRAC	1 cut(s)	2305
Mph1103I	ATGCAT	2 cut(s)	116, 1519
MroI	TCCGGA	1 cut(s)	1845
MroXI	GAANNNNTTC	1 cut(s)	2127
MslI	CAYNNNNRTG	4 cut(s)	690, 739, 1218, 3191
MspA1I	CMGCKG	1 cut(s)	2275
MspI	CCGG	6 cut(s)	925, 1062, 1846, 3656, 3814, 3873
MspR9I	CCNGG	7 cut(s)	298, 458, 561, 1352, 1962, 3166, 3656
MunI	CAATTG	2 cut(s)	2675, 2769
Mva1269I	GAATGC	1 cut(s)	3133
MvaI	CCWGG	6 cut(s)	298, 458, 561, 1352, 1962, 3166
MvnI	CGCG	3 cut(s)	2292, 3452, 4158
NciI	CCSGG	1 cut(s)	3656
NmeAIII	GCCGAG	2 cut(s)	227, 3332
NmuCI	GTSAC	7 cut(s)	629, 659, 1098, 2219, 2419, 2587, 2705
NsbI	TGCGCA	1 cut(s)	809
NsiI	ATGCAT	2 cut(s)	116, 1519
NspI	RCATGY	3 cut(s)	1665, 1691, 3637
NspV	TTCGAA	1 cut(s)	1079
OliI	CACNNNNGTG	1 cut(s)	1218
PaeI	GCATGC	1 cut(s)	1665
PagI	TCATGA	3 cut(s)	685, 1230, 3922
PauI	GCGCGC	1 cut(s)	4156
PceI	AGGCCT	1 cut(s)	375
PciSI	GCTCTTC	4 cut(s)	503, 1591, 3336, 4167
PctI	GAATGC	1 cut(s)	3133
PdmI	GAANNNNTTC	1 cut(s)	2127
PfeI	GAWTC	9 cut(s)	59, 68, 97, 1091, 2251, 2895, 3939, 4262, 4442
PfoI	TCCNGGA	1 cut(s)	559
PleI	GAGTC	6 cut(s)	656, 2811, 3013, 3307, 3563, 4085
PpsI	GAGTC	6 cut(s)	656, 2811, 3013, 3307, 3563, 4085
Ppu21I	YACGTR	1 cut(s)	329
PpuMI	RGGWCCY	2 cut(s)	1735, 2197
PshBI	ATTAAT	1 cut(s)	117
Psp5II	RGGWCCY	2 cut(s)	1735, 2197
Psp6I	CCWGG	6 cut(s)	296, 456, 559, 1350, 1960, 3164
PspEI	GGTNACC	1 cut(s)	2219
PspFI	CCCAGC	1 cut(s)	888
PspGI	CCWGG	6 cut(s)	296, 456, 559, 1350, 1960, 3164
PspPI	GGNCC	5 cut(s)	986, 1735, 2197, 2947, 3997
PspPPI	RGGWCCY	2 cut(s)	1735, 2197
PstI	CTGCAG	3 cut(s)	3472, 4239, 4441
PstNI	CAGNNNCTG	1 cut(s)	3302
PsuI	RGATCY	5 cut(s)	2100, 2277, 2481, 2924, 3079
PteI	GCGCGC	1 cut(s)	4156
RseI	CAYNNNNRTG	4 cut(s)	690, 739, 1218, 3191
SapI	GCTCTTC	4 cut(s)	503, 1591, 3336, 4167
Sau96I	GGNCC	5 cut(s)	986, 1735, 2197, 2947, 3997
ScaI	AGTACT	5 cut(s)	540, 1609, 2412, 3224, 4418
SchI	GAGTC	6 cut(s)	656, 2812, 3013, 3308, 3563, 4085
ScrFI	CCNGG	7 cut(s)	298, 458, 561, 1352, 1962, 3166, 3656
SduI	GDGCHC	4 cut(s)	165, 1069, 3004, 3590
SexAI	ACCWGGT	1 cut(s)	1960
SfaNI	GCATC	8 cut(s)	417, 462, 1240, 2171, 2296, 2338, 2515, 4214
SfcI	CTRYAG	6 cut(s)	3215, 3468, 3591, 3912, 4235, 4437
SfuI	TTCGAA	1 cut(s)	1079
SinI	GGWCC	4 cut(s)	986, 1735, 2197, 3997
SmiMI	CAYNNNNRTG	4 cut(s)	690, 739, 1218, 3191
SmlI	CTYRAG	7 cut(s)	1452, 1466, 1814, 2174, 2201, 2951, 3954
SmoI	CTYRAG	7 cut(s)	1452, 1466, 1814, 2174, 2201, 2951, 3954
SpeI	ACTAGT	1 cut(s)	2188
SphI	GCATGC	1 cut(s)	1665
SseBI	AGGCCT	1 cut(s)	375
SsiI	CCGC	8 cut(s)	1766, 2275, 2866, 2901, 3452, 3680, 3727, 4369
SspI	AATATT	2 cut(s)	2056, 2128
SspMI	CTAG	9 cut(s)	221, 542, 1302, 1473, 1487, 2189, 2619, 3387, 3645
StuI	AGGCCT	1 cut(s)	375
StyD4I	CCNGG	7 cut(s)	296, 456, 559, 1350, 1960, 3164, 3654
StyI	CCWWGG	2 cut(s)	1368, 1782
TaiI	ACGT	1 cut(s)	331
TatI	WGTACW	9 cut(s)	279, 538, 1607, 2410, 2760, 3222, 3757, 4268, 4416
TfiI	GAWTC	9 cut(s)	59, 68, 97, 1091, 2251, 2895, 3939, 4262, 4442
TscAI	CASTG	6 cut(s)	634, 1115, 2344, 3035, 3590, 3829
TseFI	GTSAC	7 cut(s)	629, 659, 1098, 2219, 2419, 2587, 2705
Tsp45I	GTSAC	7 cut(s)	629, 659, 1098, 2219, 2419, 2587, 2705
TspGWI	ACGGA	2 cut(s)	1328, 1671
TspRI	CASTG	6 cut(s)	634, 1115, 2344, 3035, 3590, 3829
VneI	GTGCAC	2 cut(s)	3000, 3586
VpaK11BI	GGWCC	4 cut(s)	986, 1735, 2197, 3997
VspI	ATTAAT	1 cut(s)	117
XagI	CCTNNNNNAGG	1 cut(s)	3699
XapI	RAATTY	9 cut(s)	301, 748, 1677, 1808, 1915, 1945, 2034, 2528, 3721
XbaI	TCTAGA	1 cut(s)	2618
XceI	RCATGY	3 cut(s)	1665, 1691, 3637
XmiI	GTMKAC	2 cut(s)	2782, 3833
XmnI	GAANNNNTTC	1 cut(s)	2127
XspI	CTAG	9 cut(s)	221, 542, 1302, 1473, 1487, 2189, 2619, 3387, 3645
ZrmI	AGTACT	5 cut(s)	540, 1609, 2412, 3224, 4418
Zsp2I	ATGCAT	2 cut(s)	116, 1519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.