Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0005937

Physical Location & Seq

Genomic Coordinates Forward (+)

12046786 .. 12050740

Gene Structure 3955 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032203

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2139 bp

ATGAAAGGGATCACACCAGAAGAAGTTGCAGATGAATATCTTATGGAGCTCTGTTTCCGTAGCATGTTACAAGTTGTTGAGAGATATGATAAGGGAAGGCCGAAACAAGTTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTTGACAGCCGAGAAGGAAAAGTTTGGTCTTATATATGACGGGAGAGAAGGAATGGAAAACATCTCAACCGTCGCTTATCAATTCACAAAATGGACAGAGAAATTAAATCATGGCCGGGAGGATGTCCAAATTGATAAACTGCCGAGTGCAGTTGTTGACTTGTTCAACTTGAGATACTTAAATTTGAAGGGCACTTTAATCGAGGAACTTTTAGGGTCCATAGGGCGTCTCCGCAACCTTCAATTTCTGAACATCAAGCATACTAAGATAGAGTCACTTCCACAAGGAATTACAAAGTTGTTAAACCTGCGCCATCTAATTATGTATTGCTACATTGGAGACTATCTGGACGACAAACTTGCGAGTGGGACAAAAGCGCCATCAAATATTTGTCAAGGCTTTGTAAACCTAGAGAGGTTGGAGTTGTTGAATTTTGCCTCGGTGAAAAGGATAACTATAGAAAAAGGGGTGATGCCAAATCTCCGCTACTTATGGATTAACCACTTCATGGAATTGAAGACAGTGCCACTGGGCCTTGAACACCTTTCTAATCTACAAACTTTGGGTCTGGAAGATGTTCCCATAGAAGTTATAAGGCTCATTCGGAAAGGAGGTGTGGATCGCTCTAAGGAACTGAAACGCTTATATTCCATTACCAGATCTCCTATTTATGCACATTTTGGAGAAGCATTAAATGGTTTATCATCAATTCGTGCCTATCAAGCTTATGGTCGGATGGCAAACACTTGTGGAAGGGCTGTGGATAATAATATCAGATTCAGTCTGCTGCTTGGTGGTTCAAACTGCTGGCTTTCTATAAGGTTGGAAACACTAGGAGGTATAATGATTTGGGTGACAGCAACCTTTGCTGTCATGCAAAATGGAAGAGCAGAAAACCAAGTACAATTTGCATCTACAATCGGTCTACTTCTCACTTACACTTTTAATATCACAAGTCTGTTGAGTGATGTTCTGAGACAAGCAAGCAGGGCAGAACATAGTTTAAATGCTGTTGAGCGGGTTGGAACATATATAGATTTGCCTTCTAAGGCTCCAGCTGTTATTGAAAGCAACCGTCCCCCACATGGGTTGCCATCATCAGGATCAATCAAGTTTGAGGATGTTGTCCTCTGTTATAGGCCTGGACTCCCACGGTCCCACCAGCGCTGCACGGATTATCTTTTACAGTTTCTGCAATTTGGACTGGAAGATTTACGAAAAGTTCTTAGCAACATACCACAATTACCGGTTCTTTTCTCAGGAACTGTGCGGTTTAATCTTGATCCTTTCAGTGAGCATAATGATGCTGACCTTTGGGAAGCCCTAGAAATGGCGCATCTAAAGGATGTCATTACGAGGAATTCATTGATTTCATTCTGTCTCGATGCTGAGGTTTCCGAGGGCGGTGAAAATTTCAGTGTTGGACAGAGGCAGCTAACAAGTCTTGCTAGAGCATTGCTACGGAGATCAAAGATTCTTGTTCTTGATGAAGCAACTACAGCTGTCGATGTTAGAACTGATGCTCTCATCCAAAAGACCATACGTGAAGAATTCAAATCTTGCACCATGCTCGTTATTGCTCACAGATTGAACACCATTATCGACTGTGATCGAATTCTTGTTCTTGATGCTGGTCAGGTCCTAGAACATGGTTCCCCAGAAGAACTACTGTTAAATGCAGATAGCCCATTCTCCAAAATGGTCAGTAGTATAGGACCTTGTAATGCTCAATACTTACGCAACCTGGTATTCGAAGGCAAGCAGAATAAGGTGAACAGAGACCAGCAACTGGTTGGCCAGAGCCGCAGGAGATGGTTAGCCTCTTCACGCTGGGCAGCTGCTGCCCAATTTGCTTTGACCGTTAGTGTCGCATCGTCACAGAGCGATCTCCAAAGGTTAGATATTGTAGACGACGACAATATCCTCATGAAAACAAAGGATGCAGTTATAACACTACAGAGGGAGTTTTGGAAGGAAGGCATGATAAAAATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0008150 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04976 map01523 map01524 map02010 map04976

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

713

Amino Acids

80.48

Weight (kDa)

7.92

Isoelectric Point (pI)

47.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_DRP	PF23559	4 - 47	5.4e-09	Disease resistance protein Winged helix domain
LRR_14	PF23598	99 - 157	1.4e-12	Leucine-rich repeat region
ABC_tran	PF00005	451 - 548	4.4e-09	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005937)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr2g0097901 RchiOBHm_Chr3g0492001
rosa_laevigata	RLG00000026535 RLG00000032203
rosa_multiflora	Rmu_co8080046.1_g000001 Rmu_sc0001501.1_g000097 Rmu_sc0005017.1_g000007 Rmu_sc0033460.1_g000001
rosa_roxburghii	Rroxscaffold_1G00035940 Rroxscaffold_3G00254760 Rroxscaffold_6G00392650
rosa_rugosa	Rorug07G0277500 Rorug07G0277700
rosa_samantha	Rh3AG301300 Rh3AG301400 Rh3BG337300 Rh3CG334500 Rh3CG334600 Rh3CG334700 Rh3DG336800 Rh5AG121500 Rh5BG119900 Rh5DG119100 Rh5DG242800 Rh7AG432700 Rh7BG405500 Rh7BG405600 Rh7CG452000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	737, 2093
Acc36I	ACCTGC	1 cut(s)	459
AccB7I	CCANNNNNTGG	2 cut(s)	652, 1933
AccBSI	CCGCTC	1 cut(s)	1162
AccI	GTMKAC	2 cut(s)	1069, 2052
AciI	CCGC	6 cut(s)	376, 628, 1162, 1414, 1548, 1948
AclWI	GGATC	4 cut(s)	17, 771, 1255, 1421
AcoI	YGGCCR	2 cut(s)	256, 1939
AcsI	RAATTY	6 cut(s)	325, 574, 1504, 1555, 1694, 1758
AcyI	GRCGYC	1 cut(s)	370
AfaI	GTAC	1 cut(s)	1047
AfeI	AGCGCT	1 cut(s)	1310
AfiI	CCNNNNNNNGG	6 cut(s)	652, 1229, 1230, 1244, 1474, 1933
AgeI	ACCGGT	1 cut(s)	1390
AjnI	CCWGG	2 cut(s)	1285, 1887
AluBI	AGCT	7 cut(s)	49, 136, 869, 1202, 1579, 1646, 1982
AluI	AGCT	7 cut(s)	49, 136, 869, 1202, 1579, 1646, 1982
Alw21I	GWGCWC	1 cut(s)	51
Alw26I	GTCTC	5 cut(s)	377, 477, 1114, 1529, 1917
AlwI	GGATC	4 cut(s)	17, 771, 1255, 1421
AlwNI	CAGNNNCTG	4 cut(s)	1336, 1409, 1933, 1985
Aor51HI	AGCGCT	1 cut(s)	1310
AoxI	GGCC	5 cut(s)	98, 256, 676, 1283, 1939
ApeKI	GCWGC	6 cut(s)	931, 1311, 1576, 1979, 1982, 1985
ApoI	RAATTY	6 cut(s)	325, 574, 1504, 1555, 1694, 1758
ArsI	GACNNNNNNTTYG	2 cut(s)	694, 726
AsiGI	ACCGGT	1 cut(s)	1390
Asp700I	GAANNNNTTC	1 cut(s)	720
AspLEI	GCGC	4 cut(s)	456, 523, 1311, 1480
AspS9I	GGNCC	5 cut(s)	360, 676, 1299, 1783, 1859
AsuC2I	CCSGG	1 cut(s)	260
AsuHPI	GGTGA	5 cut(s)	598, 625, 1009, 1562, 1927
AsuII	TTCGAA	1 cut(s)	1896
AvaII	GGWCC	4 cut(s)	360, 1299, 1783, 1859
BaeGI	GKGCMC	1 cut(s)	338
BalI	TGGCCA	1 cut(s)	1941
BanII	GRGCYC	1 cut(s)	51
BbsI	GAAGAC	1 cut(s)	668
Bbv12I	GWGCWC	1 cut(s)	51
BbvCI	CCTCAGC	1 cut(s)	1533
BbvI	GCAGC	6 cut(s)	918, 1298, 1588, 1969, 1972, 1991
BccI	CCATC	5 cut(s)	465, 532, 874, 1246, 1950
BcgI	CGANNNNNNTGC	6 cut(s)	325, 359, 485, 519, 1696, 1730
BciT130I	CCWGG	2 cut(s)	1287, 1889
BcnI	CCSGG	1 cut(s)	260
BcoDI	GTCTC	5 cut(s)	377, 477, 1114, 1529, 1917
BfaI	CTAG	5 cut(s)	554, 977, 1469, 1593, 1787
BfmI	CTRYAG	3 cut(s)	600, 1641, 2099
BfoI	RGCGCY	2 cut(s)	524, 1312
BfuAI	ACCTGC	1 cut(s)	459
BglII	AGATCT	1 cut(s)	803
BisI	GCNGC	7 cut(s)	932, 1312, 1577, 1948, 1980, 1983, 1986
BlsI	GCNGC	7 cut(s)	933, 1313, 1578, 1949, 1981, 1984, 1987
Bme1390I	CCNGG	3 cut(s)	260, 1287, 1889
Bme18I	GGWCC	4 cut(s)	360, 1299, 1783, 1859
BmgT120I	GGNCC	5 cut(s)	360, 676, 1299, 1783, 1859
BmiI	GGNNCC	4 cut(s)	361, 1197, 1301, 1798
BmrFI	CCNGG	3 cut(s)	260, 1287, 1889
BmrI	ACTGGG	1 cut(s)	683
BmuI	ACTGGG	1 cut(s)	683
BpiI	GAAGAC	1 cut(s)	668
BpmI	CTGGAG	1 cut(s)	1182
Bpu10I	CCTNAGC	1 cut(s)	1533
Bpu14I	TTCGAA	1 cut(s)	1896
BpuEI	CTTGAG	1 cut(s)	334
BpuMI	CCSGG	1 cut(s)	260
BsaAI	YACGTR	1 cut(s)	1688
BsaBI	GATNNNNATC	1 cut(s)	36
BsaHI	GRCGYC	1 cut(s)	370
BsaI	GGTCTC	1 cut(s)	1917
BsaJI	CCNNGG	3 cut(s)	582, 1295, 1542
BsaWI	WCCGGW	1 cut(s)	1390
BsaXI	ACNNNNNCTCC	2 cut(s)	790, 820
Bsc4I	CCNNNNNNNGG	6 cut(s)	652, 1229, 1230, 1244, 1474, 1933
Bse118I	RCCGGY	1 cut(s)	1390
Bse1I	ACTGG	3 cut(s)	678, 1353, 1938
Bse3DI	GCAATG	1 cut(s)	1598
Bse8I	GATNNNNATC	1 cut(s)	36
BseBI	CCWGG	2 cut(s)	1287, 1889
BseDI	CCNNGG	3 cut(s)	582, 1295, 1542
BseGI	GGATG	6 cut(s)	271, 885, 1270, 1495, 1671, 2089
BseJI	GATNNNNATC	1 cut(s)	36
BseLI	CCNNNNNNNGG	6 cut(s)	652, 1229, 1230, 1244, 1474, 1933
BseMI	GCAATG	1 cut(s)	1598
BseMII	CTCAG	3 cut(s)	1109, 1416, 1524
BseNI	ACTGG	3 cut(s)	678, 1353, 1938
BseSI	GKGCMC	1 cut(s)	338
BseXI	GCAGC	6 cut(s)	918, 1298, 1588, 1969, 1972, 1991
BseYI	CCCAGC	1 cut(s)	1974
BsgI	GTGCAG	2 cut(s)	312, 1297
BshFI	GGCC	5 cut(s)	100, 258, 678, 1285, 1941
BshTI	ACCGGT	1 cut(s)	1390
BsiHKAI	GWGCWC	1 cut(s)	51
BsiSI	CCGG	2 cut(s)	259, 1391
BslFI	GGGAC	3 cut(s)	526, 1206, 1285
BslI	CCNNNNNNNGG	6 cut(s)	652, 1229, 1230, 1244, 1474, 1933
BsmAI	GTCTC	5 cut(s)	377, 477, 1114, 1529, 1917
BsmBI	CGTCTC	1 cut(s)	377
BsmFI	GGGAC	3 cut(s)	526, 1206, 1285
BsnI	GGCC	5 cut(s)	100, 258, 678, 1285, 1941
Bso31I	GGTCTC	1 cut(s)	1917
Bsp119I	TTCGAA	1 cut(s)	1896
Bsp1286I	GDGCHC	2 cut(s)	51, 338
Bsp143I	GATC	9 cut(s)	9, 121, 763, 803, 1247, 1426, 1610, 1753, 2029
BspACI	CCGC	6 cut(s)	376, 628, 1162, 1414, 1548, 1948
BspANI	GGCC	5 cut(s)	100, 258, 678, 1285, 1941
BspCNI	CTCAG	3 cut(s)	1110, 1415, 1525
BspHI	TCATGA	1 cut(s)	2070
BspLI	GGNNCC	4 cut(s)	361, 1197, 1301, 1798
BspMI	ACCTGC	1 cut(s)	459
BspPI	GGATC	4 cut(s)	17, 771, 1255, 1421
BspQI	GCTCTTC	1 cut(s)	1024
BspT104I	TTCGAA	1 cut(s)	1896
BspTNI	GGTCTC	1 cut(s)	1917
BsrBI	CCGCTC	1 cut(s)	1162
BsrDI	GCAATG	1 cut(s)	1598
BsrFI	RCCGGY	1 cut(s)	1390
BsrI	ACTGG	3 cut(s)	678, 1353, 1938
BssAI	RCCGGY	1 cut(s)	1390
BssECI	CCNNGG	3 cut(s)	582, 1295, 1542
BssMI	GATC	9 cut(s)	9, 121, 763, 803, 1247, 1426, 1610, 1753, 2029
BssNI	GRCGYC	1 cut(s)	370
Bst2UI	CCWGG	2 cut(s)	1287, 1889
Bst4CI	ACNGT	9 cut(s)	214, 667, 1220, 1299, 1332, 1411, 1751, 1815, 2005
Bst6I	CTCTTC	2 cut(s)	1024, 1972
BstACI	GRCGYC	1 cut(s)	370
BstAPI	GCANNNNNTGC	2 cut(s)	1010, 1985
BstBAI	YACGTR	1 cut(s)	1688
BstBI	TTCGAA	1 cut(s)	1896
BstC8I	GCNNGC	4 cut(s)	138, 953, 1129, 1904
BstDEI	CTNAG	7 cut(s)	408, 771, 1118, 1191, 1370, 1402, 1533
BstDSI	CCRYGG	1 cut(s)	1295
BstF5I	GGATG	6 cut(s)	271, 885, 1270, 1495, 1671, 2089
BstH2I	RGCGCY	2 cut(s)	524, 1312
BstHHI	GCGC	4 cut(s)	456, 523, 1311, 1480
BstKTI	GATC	9 cut(s)	12, 124, 766, 806, 1250, 1429, 1613, 1756, 2032
BstMAI	GTCTC	5 cut(s)	377, 477, 1114, 1529, 1917
BstMBI	GATC	9 cut(s)	9, 121, 763, 803, 1247, 1426, 1610, 1753, 2029
BstMWI	GCNNNNNNNGC	7 cut(s)	866, 1010, 1133, 1643, 1947, 1985, 1994
BstNI	CCWGG	2 cut(s)	1287, 1889
BstNSI	RCATGY	1 cut(s)	67
BstSCI	CCNGG	3 cut(s)	258, 1285, 1887
BstSFI	CTRYAG	3 cut(s)	600, 1641, 2099
BstSLI	GKGCMC	1 cut(s)	338
BstV1I	GCAGC	6 cut(s)	918, 1298, 1588, 1969, 1972, 1991
BstV2I	GAAGAC	1 cut(s)	668
BstX2I	RGATCY	1 cut(s)	803
BstYI	RGATCY	1 cut(s)	803
BsuRI	GGCC	5 cut(s)	100, 258, 678, 1285, 1941
BtgI	CCRYGG	1 cut(s)	1295
BtsCI	GGATG	6 cut(s)	271, 885, 1270, 1495, 1671, 2089
BtsIMutI	CAGTG	4 cut(s)	671, 672, 1441, 1567
BveI	ACCTGC	1 cut(s)	459
Cac8I	GCNNGC	4 cut(s)	138, 953, 1129, 1904
CaiI	CAGNNNCTG	4 cut(s)	1336, 1409, 1933, 1985
CciI	TCATGA	1 cut(s)	2070
CfoI	GCGC	4 cut(s)	456, 523, 1311, 1480
Cfr10I	RCCGGY	1 cut(s)	1390
Cfr13I	GGNCC	5 cut(s)	360, 676, 1299, 1783, 1859
CseI	GACGC	1 cut(s)	359
CsiI	ACCWGGT	1 cut(s)	1887
Csp6I	GTAC	1 cut(s)	1046
CspAI	ACCGGT	1 cut(s)	1390
CspCI	CAANNNNNGTGG	2 cut(s)	1215, 1250
CviQI	GTAC	1 cut(s)	1046
DdeI	CTNAG	7 cut(s)	408, 771, 1118, 1191, 1370, 1402, 1533
DpnI	GATC	9 cut(s)	11, 123, 765, 805, 1249, 1428, 1612, 1755, 2031
DpnII	GATC	9 cut(s)	9, 121, 763, 803, 1247, 1426, 1610, 1753, 2029
DraI	TTTAAA	1 cut(s)	1149
EaeI	YGGCCR	2 cut(s)	256, 1939
Eam1104I	CTCTTC	2 cut(s)	1024, 1972
EarI	CTCTTC	2 cut(s)	1024, 1972
Ecl136II	GAGCTC	1 cut(s)	49
Eco147I	AGGCCT	1 cut(s)	1285
Eco24I	GRGCYC	1 cut(s)	51
Eco31I	GGTCTC	1 cut(s)	1917
Eco47I	GGWCC	4 cut(s)	360, 1299, 1783, 1859
Eco47III	AGCGCT	1 cut(s)	1310
Eco53kI	GAGCTC	1 cut(s)	49
EcoICRI	GAGCTC	1 cut(s)	49
EcoO109I	RGGNCCY	2 cut(s)	1783, 1859
EcoRI	GAATTC	3 cut(s)	1504, 1694, 1758
EcoRII	CCWGG	2 cut(s)	1285, 1887
EcoT22I	ATGCAT	1 cut(s)	120
EcoT38I	GRGCYC	1 cut(s)	51
Esp3I	CGTCTC	1 cut(s)	377
FaqI	GGGAC	3 cut(s)	526, 1206, 1285
FauI	CCCGC	1 cut(s)	1155
FblI	GTMKAC	2 cut(s)	1069, 2052
Fnu4HI	GCNGC	7 cut(s)	932, 1312, 1577, 1948, 1980, 1983, 1986
FokI	GGATG	6 cut(s)	278, 892, 1277, 1502, 1658, 2096
FriOI	GRGCYC	1 cut(s)	51
Fsp4HI	GCNGC	7 cut(s)	932, 1312, 1577, 1948, 1980, 1983, 1986
FspBI	CTAG	5 cut(s)	554, 977, 1469, 1593, 1787
GlaI	GCGC	4 cut(s)	455, 522, 1310, 1479
GluI	GCNGC	7 cut(s)	932, 1312, 1577, 1948, 1980, 1983, 1986
GsaI	CCCAGC	1 cut(s)	1978
GsuI	CTGGAG	1 cut(s)	1182
HaeII	RGCGCY	2 cut(s)	524, 1312
HaeIII	GGCC	5 cut(s)	100, 258, 678, 1285, 1941
HapII	CCGG	2 cut(s)	259, 1391
HgaI	GACGC	1 cut(s)	359
HhaI	GCGC	4 cut(s)	456, 523, 1311, 1480
Hin1I	GRCGYC	1 cut(s)	370
Hin6I	GCGC	4 cut(s)	454, 521, 1309, 1478
HinP1I	GCGC	4 cut(s)	454, 521, 1309, 1478
HincII	GTYRAC	2 cut(s)	147, 301
HindII	GTYRAC	2 cut(s)	147, 301
HindIII	AAGCTT	1 cut(s)	867
HinfI	GANTC	4 cut(s)	416, 921, 1290, 1618
HpaII	CCGG	2 cut(s)	259, 1391
HphI	GGTGA	5 cut(s)	598, 625, 1009, 1562, 1927
Hpy166II	GTNNAC	6 cut(s)	147, 301, 550, 1070, 1918, 2053
Hpy188I	TCNGA	7 cut(s)	126, 393, 750, 879, 920, 1119, 1543
Hpy188III	TCNNGA	9 cut(s)	491, 713, 1245, 1404, 1424, 1526, 1628, 1769, 2071
Hpy8I	GTNNAC	6 cut(s)	147, 301, 550, 1070, 1918, 2053
Hpy99I	CGWCG	2 cut(s)	218, 2060
HpyCH4III	ACNGT	9 cut(s)	214, 667, 1220, 1299, 1332, 1411, 1751, 1815, 2005
HpyCH4IV	ACGT	1 cut(s)	1687
HpyF10VI	GCNNNNNNNGC	7 cut(s)	866, 1010, 1133, 1643, 1947, 1985, 1994
HpyF3I	CTNAG	7 cut(s)	408, 771, 1118, 1191, 1370, 1402, 1533
HpySE526I	ACGT	1 cut(s)	1687
Hsp92I	GRCGYC	1 cut(s)	370
HspAI	GCGC	4 cut(s)	454, 521, 1309, 1478
Kzo9I	GATC	9 cut(s)	9, 121, 763, 803, 1247, 1426, 1610, 1753, 2029
LguI	GCTCTTC	1 cut(s)	1024
LmnI	GCTCC	2 cut(s)	46, 1201
Lsp1109I	GCAGC	6 cut(s)	918, 1298, 1588, 1969, 1972, 1991
MabI	ACCWGGT	1 cut(s)	1887
MaeI	CTAG	5 cut(s)	554, 977, 1469, 1593, 1787
MaeII	ACGT	1 cut(s)	1687
MaeIII	GTNAC	4 cut(s)	66, 417, 997, 2019
MalI	GATC	9 cut(s)	11, 123, 765, 805, 1249, 1428, 1612, 1755, 2031
MbiI	CCGCTC	1 cut(s)	1162
MboI	GATC	9 cut(s)	9, 121, 763, 803, 1247, 1426, 1610, 1753, 2029
MboII	GAAGA	8 cut(s)	32, 673, 728, 1041, 1364, 1703, 1817, 1959
MflI	RGATCY	1 cut(s)	803
MhlI	GDGCHC	2 cut(s)	51, 338
MlsI	TGGCCA	1 cut(s)	1941
MluNI	TGGCCA	1 cut(s)	1941
MlyI	GAGTC	2 cut(s)	425, 1284
MmeI	TCCRAC	5 cut(s)	543, 857, 948, 1147, 1546
Mox20I	TGGCCA	1 cut(s)	1941
Mph1103I	ATGCAT	1 cut(s)	120
MroXI	GAANNNNTTC	1 cut(s)	720
MscI	TGGCCA	1 cut(s)	1941
MslI	CAYNNNNRTG	1 cut(s)	1446
Msp20I	TGGCCA	1 cut(s)	1941
MspA1I	CMGCKG	3 cut(s)	1202, 1646, 1982
MspI	CCGG	2 cut(s)	259, 1391
MspR9I	CCNGG	3 cut(s)	260, 1287, 1889
MvaI	CCWGG	2 cut(s)	1287, 1889
MwoI	GCNNNNNNNGC	7 cut(s)	866, 1010, 1133, 1643, 1947, 1985, 1994
NciI	CCSGG	1 cut(s)	260
NdeII	GATC	9 cut(s)	9, 121, 763, 803, 1247, 1426, 1610, 1753, 2029
NlaIV	GGNNCC	4 cut(s)	361, 1197, 1301, 1798
NmeAIII	GCCGAG	2 cut(s)	178, 312
NmuCI	GTSAC	3 cut(s)	417, 997, 2019
NsiI	ATGCAT	1 cut(s)	120
NspI	RCATGY	1 cut(s)	67
NspV	TTCGAA	1 cut(s)	1896
PagI	TCATGA	1 cut(s)	2070
PceI	AGGCCT	1 cut(s)	1285
PciSI	GCTCTTC	1 cut(s)	1024
PdmI	GAANNNNTTC	1 cut(s)	720
PfeI	GAWTC	2 cut(s)	921, 1618
PflMI	CCANNNNNTGG	2 cut(s)	652, 1933
PinAI	ACCGGT	1 cut(s)	1390
PkrI	GCNGC	7 cut(s)	933, 1313, 1578, 1949, 1981, 1984, 1987
PleI	GAGTC	2 cut(s)	424, 1284
PpsI	GAGTC	2 cut(s)	424, 1284
Ppu21I	YACGTR	1 cut(s)	1688
PpuMI	RGGWCCY	2 cut(s)	1783, 1859
PsiI	TTATAA	2 cut(s)	737, 2093
Psp124BI	GAGCTC	1 cut(s)	51
Psp5II	RGGWCCY	2 cut(s)	1783, 1859
Psp6I	CCWGG	2 cut(s)	1285, 1887
PspFI	CCCAGC	1 cut(s)	1974
PspGI	CCWGG	2 cut(s)	1285, 1887
PspN4I	GGNNCC	4 cut(s)	361, 1197, 1301, 1798
PspPI	GGNCC	5 cut(s)	360, 676, 1299, 1783, 1859
PspPPI	RGGWCCY	2 cut(s)	1783, 1859
PstNI	CAGNNNCTG	4 cut(s)	1336, 1409, 1933, 1985
PsuI	RGATCY	1 cut(s)	803
PvuII	CAGCTG	3 cut(s)	1202, 1646, 1982
RsaI	GTAC	1 cut(s)	1047
RsaNI	GTAC	1 cut(s)	1046
RseI	CAYNNNNRTG	1 cut(s)	1446
SacI	GAGCTC	1 cut(s)	51
SapI	GCTCTTC	1 cut(s)	1024
SatI	GCNGC	7 cut(s)	932, 1312, 1577, 1948, 1980, 1983, 1986
Sau3AI	GATC	9 cut(s)	9, 121, 763, 803, 1247, 1426, 1610, 1753, 2029
Sau96I	GGNCC	5 cut(s)	360, 676, 1299, 1783, 1859
SchI	GAGTC	2 cut(s)	425, 1284
ScrFI	CCNGG	3 cut(s)	260, 1287, 1889
SduI	GDGCHC	2 cut(s)	51, 338
SexAI	ACCWGGT	1 cut(s)	1887
SfcI	CTRYAG	3 cut(s)	600, 1641, 2099
SfuI	TTCGAA	1 cut(s)	1896
SinI	GGWCC	4 cut(s)	360, 1299, 1783, 1859
SmiMI	CAYNNNNRTG	1 cut(s)	1446
SmlI	CTYRAG	1 cut(s)	313
SmoI	CTYRAG	1 cut(s)	313
SseBI	AGGCCT	1 cut(s)	1285
SsiI	CCGC	6 cut(s)	376, 628, 1162, 1414, 1548, 1948
SspI	AATATT	1 cut(s)	532
SspMI	CTAG	5 cut(s)	554, 977, 1469, 1593, 1787
SstI	GAGCTC	1 cut(s)	51
StuI	AGGCCT	1 cut(s)	1285
StyD4I	CCNGG	3 cut(s)	258, 1285, 1887
TaaI	ACNGT	9 cut(s)	214, 667, 1220, 1299, 1332, 1411, 1751, 1815, 2005
TaiI	ACGT	1 cut(s)	1690
TaqI	TCGA	6 cut(s)	345, 1527, 1650, 1746, 1756, 1896
TaqII	GACCGA	1 cut(s)	1055
TatI	WGTACW	1 cut(s)	1045
TauI	GCSGC	1 cut(s)	1950
TfiI	GAWTC	2 cut(s)	921, 1618
TscAI	CASTG	4 cut(s)	672, 678, 1441, 1567
TseFI	GTSAC	3 cut(s)	417, 997, 2019
TseI	GCWGC	6 cut(s)	931, 1311, 1576, 1979, 1982, 1985
Tsp45I	GTSAC	3 cut(s)	417, 997, 2019
TspDTI	ATGAA	7 cut(s)	17, 48, 640, 1497, 1506, 1647, 2087
TspGWI	ACGGA	3 cut(s)	47, 1331, 1621
TspRI	CASTG	4 cut(s)	672, 678, 1441, 1567
Van91I	CCANNNNNTGG	2 cut(s)	652, 1933
VpaK11BI	GGWCC	4 cut(s)	360, 1299, 1783, 1859
XapI	RAATTY	6 cut(s)	325, 574, 1504, 1555, 1694, 1758
XceI	RCATGY	1 cut(s)	67
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1934
XmiI	GTMKAC	2 cut(s)	1069, 2052
XmnI	GAANNNNTTC	1 cut(s)	720
XspI	CTAG	5 cut(s)	554, 977, 1469, 1593, 1787
Zsp2I	ATGCAT	1 cut(s)	120

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.