Rmu_sc0000240.1_g000076
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000240.1
OG0002297
Physical Location & Seq
Reverse (-)
315265 .. 323080
7816 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2994 bp
atgttgagactgatattttggggttgcctcattggtctggcttgttcttgcttcttcctagttgcggttgcacaagttactaatccttctgaagtggatgcattgagagcagtgaaggatagtctgattgattccgggaagcatttggggaattgggacaaaggggatccatgtaaagcgaattggaccggagttttgtgctccggtgatgttgggactgatggatacttgcatgtccaggagctgcaactgctaagtaagaatctctcaggaactttagcgcctgagcttggcaaactatctcaacttctgatattagatttcatgtggaataacttaagtgggactataccgaaggagataggaaacattacgtccttgaaactcttgctcttgaatggaaacaaactatcgggttccttgcctgatgagctcggtaatcttgaaaatctgcatagactacaagttgaccagaaccatctttcaggttcaataccaaaatcatttgttaacttgcgcggcttaagacacctccatatgaacaacaactccttcagtggccaaattccatctgagatttctgaaatacccgctcttcttcatgtgctcttggataataataaattatctggatatcttccacaagaattctccaacattccagggttgcgcatacttcaactcgataacaacaacttcatgggaactgagattccagcaacttatggaaacctttccagattggcaaagttaagtctcaggaactgcagtttgcagggagcaattcctgatcttagccggatacctaacctcagttatttagatctaagctggaatcatcttgctggaccaataccatcacatggactttctgacaatatgacaacaattgacctgtcagataatcatcttaacggatctattcctgaaggtttctctcattttcctcatcttcagaaattgtcgctcgaaaataattggttgaacggttctgtccctgctatatggaggaacatatcttttagtacaagagctaaactttcatatgatctccggaacaattcactctctaacatattgggagagctgaatcctccagcaaatgtcaccttgaggctagaaggaaatcctatttgcaagaatgctagcatagaaaatataagccctttctgtcaatctgaagcgagagatgggatccccgaagattcaataaattcaactcaacagatgacctgccctattcaagcatgtccggtggatgatttctatgaatatgttccatcttcccctgtgccatgcttttgtgcatcacctattatagttgaatatcgtctgaaaagcccgagcttttcttactttcttccatatatacagcagtttgaagagtatttaactagttctctaaaattggagatttaccagttatcaattagttcctttgtctgggaaaaagggcctcgtttacagatgcatttgaagttcttccctgtgtatggtgcttcacactcgtataaattaaatacaagtgaggttctgcgaattagaggcatctttacatcatgggaatttcctcagaatgatttctttggaccatatgaactactcgacttcaaactgctgggaccttattcaaatatgattgtcaagagacaaaagatgggcatcaataagggtgtattagcagctgttataataggggccattgcctctgttgtcagtatatctgcaacagtcatgcttctgttcacaagatgcacccgaaaccgacatacaccaccttcaaggagacactcatcgtcgaagatttctatgagaattgaaggtgtaaaggcgttcactttcaaagaaatgacactagccaccaggaactttgatagtgcaactcaacttggacgaggaggctatgggaaagtttataaaggtatcttatctgataacacagctgtagccataaaacgtgcagaagaaggatccctacagggtgaaaaagaattcttgaccgagatagaattgttatcaaggctacaccatcgaaacttagtctctttggtaggatactgtgaagaagaaggggagcaggtggctgattttggactctcacgactagcacctgtccagaatgatgaagggactggccctgcatacgtatctacagtcgtgagaggaacaccgggctacctggatccagagtatttcttgaccagtaagttaacagacaaaagcgatgtctatagccttggaattgtgtttctggagctcttgactggtgtgctgccaatatcacatggcaaaaacattgttcgcgaggtgaatatggctcatcagtcaggcttgatgttctccattatagatagcagaatgggttcgtatccatctgaatgtgttcagaggtttgtagctttggccctcagatgttgccatgataaacaagacgaccggccatcaatgttggatgtggtgagggagctcgagaacataatcaaacttatgccgggagctgaaacaatgttttcaccatctggttcctcatacactgaaaaatcattaacttcatcatcgacttttactggggacccatatatgtcctcctccaacgtggaaggaagtgatctaaccagtggtgtggggttccttgaattcgatctggggagggtgctgtttggcagatttaacgcttacctatggcgcggcgatgacggtccggctacagtggccgtggtggggatgggccaggcttggctgacgaagcagatctcccaatacaatgtcgctacgattatcaccgactatgtcaaccaccatacgcttctatacccgacgaagaagaagaggatcgtctgtgaccaagggattcattctctattcggcaggaaaaccattgcttggatcaagatctacaatttattccagccgcatttcactgataatctcgaggatgattccgatgattcgtcggatgagtccgacgacaacggtttggatgaggaggactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

997

Amino Acids

110.62

Weight (kDa)

5.72

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 68 2e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 93 - 194 4.1e-09 Leucine-rich repeat region
LRR_14 PF23598 168 - 348 2.1e-07 Leucine-rich repeat region
Pkinase PF00069 626 - 691 4.1e-09 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 628 - 691 1.7e-10 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 693 - 823 2.1e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 694 - 821 7.4e-14 Protein kinase domain
SWIB PF02201 915 - 973 1.6e-06 SWIB/MDM2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1691, 1917
AarI CACCTGC 1 cut(s) 2068
Acc16I TGCGCA 1 cut(s) 671
Acc36I ACCTGC 2 cut(s) 1241, 2068
AccB7I CCANNNNNTGG 2 cut(s) 863, 2883
AccBSI CCGCTC 1 cut(s) 593
AccII CGCG 3 cut(s) 519, 2304, 2688
AccIII TCCGGA 1 cut(s) 1053
AciI CCGC 5 cut(s) 65, 519, 591, 2688, 2912
AcoI YGGCCR 3 cut(s) 559, 2438, 2712
AcsI RAATTY 6 cut(s) 564, 647, 1213, 1565, 1991, 2636
AcuI CTGAAG 5 cut(s) 111, 538, 938, 948, 1200
AfaI GTAC 1 cut(s) 1027
AfiI CCNNNNNNNGG 7 cut(s) 64, 290, 863, 1443, 1493, 2721, 2883
AflII CTTAAG 2 cut(s) 337, 523
AhlI ACTAGT 1 cut(s) 1394
AjnI CCWGG 5 cut(s) 237, 661, 1862, 2178, 2730
AloI GAACNNNNNNTCC 4 cut(s) 533, 565, 697, 729
Alw21I GWGCWC 5 cut(s) 203, 435, 609, 2259, 2469
Alw26I GTCTC 4 cut(s) 761, 1642, 1780, 2047
AlwNI CAGNNNCTG 2 cut(s) 244, 765
Ama87I CYCGRG 3 cut(s) 1342, 2468, 2930
Aor13HI TCCGGA 1 cut(s) 1053
AoxI GGCC 8 cut(s) 559, 1454, 1698, 2134, 2403, 2438, 2712, 2728
ApeKI GCWGC 3 cut(s) 244, 1682, 2272
ApoI RAATTY 6 cut(s) 564, 647, 1213, 1565, 1991, 2636
ArsI GACNNNNNNTTYG 5 cut(s) 32, 178, 210, 2211, 2243
Asp700I GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2362, 2382
AspLEI GCGC 4 cut(s) 283, 519, 672, 2688
AsuC2I CCSGG 3 cut(s) 136, 2172, 2493
AsuHPI GGTGA 8 cut(s) 218, 1099, 1302, 1994, 2320, 2470, 2505, 2773
AsuNHI GCTAGC 1 cut(s) 1145
AvaI CYCGRG 3 cut(s) 1342, 2468, 2930
AvaII GGWCC 6 cut(s) 186, 848, 1589, 1622, 2572, 2699
BalI TGGCCA 1 cut(s) 561
BamHI GGATCC 4 cut(s) 166, 1194, 1970, 2182
BanII GRGCYC 3 cut(s) 435, 2259, 2469
BarI GAAGNNNNNNTAC 2 cut(s) 1959, 1991
Bbv12I GWGCWC 5 cut(s) 203, 435, 609, 2259, 2469
BbvI GCAGC 3 cut(s) 231, 1694, 2259
BceAI ACGGC 1 cut(s) 2699
BciT130I CCWGG 5 cut(s) 239, 663, 1864, 2180, 2732
BciVI GTATCC 4 cut(s) 218, 795, 2048, 2379
BcnI CCSGG 3 cut(s) 136, 2172, 2493
BcoDI GTCTC 4 cut(s) 761, 1642, 1780, 2047
BcuI ACTAGT 1 cut(s) 1394
BfaI CTAG 7 cut(s) 59, 1118, 1146, 1395, 1856, 2105, 2992
BfmI CTRYAG 6 cut(s) 766, 1944, 1976, 2151, 2230, 2706
BfoI RGCGCY 1 cut(s) 284
BfrI CTTAAG 2 cut(s) 337, 523
BfuAI ACCTGC 2 cut(s) 1241, 2068
BfuI GTATCC 4 cut(s) 218, 795, 2048, 2379
BglII AGATCT 3 cut(s) 823, 2751, 2892
BisI GCNGC 6 cut(s) 245, 520, 1683, 2273, 2689, 2912
BlsI GCNGC 6 cut(s) 246, 521, 1684, 2274, 2690, 2913
Bme1390I CCNGG 8 cut(s) 136, 239, 663, 1864, 2172, 2180, 2493, 2732
Bme18I GGWCC 6 cut(s) 186, 848, 1589, 1622, 2572, 2699
BmeT110I CYCGRG 3 cut(s) 1342, 2468, 2930
BmrFI CCNGG 8 cut(s) 136, 239, 663, 1864, 2172, 2180, 2493, 2732
BmrI ACTGGG 1 cut(s) 2577
BmsI GCATC 6 cut(s) 88, 1316, 1458, 1557, 1671, 1742
BmtI GCTAGC 1 cut(s) 1149
BmuI ACTGGG 1 cut(s) 2577
BpmI CTGGAG 2 cut(s) 1080, 2273
Bpu10I CCTNAGC 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 1132
BpuMI CCSGG 3 cut(s) 136, 2172, 2493
BsaAI YACGTR 1 cut(s) 2146
BsaBI GATNNNNATC 3 cut(s) 906, 1661, 2891
BsaJI CCNNGG 4 cut(s) 662, 2236, 2715, 2845
BsaWI WCCGGW 4 cut(s) 188, 203, 1053, 1252
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 290, 863, 1443, 1493, 2721, 2883
Bse118I RCCGGY 1 cut(s) 2436
Bse1I ACTGG 6 cut(s) 1420, 2137, 2202, 2269, 2572, 2616
Bse3DI GCAATG 2 cut(s) 1701, 2877
Bse8I GATNNNNATC 3 cut(s) 906, 1661, 2891
BseAI TCCGGA 1 cut(s) 1053
BseBI CCWGG 5 cut(s) 239, 663, 1864, 2180, 2732
BseDI CCNNGG 4 cut(s) 662, 2236, 2715, 2845
BseGI GGATG 7 cut(s) 103, 1264, 2458, 2730, 2941, 2962, 2986
BseJI GATNNNNATC 3 cut(s) 906, 1661, 2891
BseLI CCNNNNNNNGG 7 cut(s) 64, 290, 863, 1443, 1493, 2721, 2883
BseMI GCAATG 2 cut(s) 1701, 2877
BseMII CTCAG 8 cut(s) 276, 282, 564, 699, 772, 826, 1586, 2422
BseNI ACTGG 6 cut(s) 1420, 2137, 2202, 2269, 2572, 2616
BseRI GAGGAG 2 cut(s) 1911, 2578
BseXI GCAGC 3 cut(s) 231, 1694, 2259
BseYI CCCAGC 1 cut(s) 1618
BsgI GTGCAG 1 cut(s) 1980
Bsh1236I CGCG 3 cut(s) 519, 2304, 2688
Bsh1285I CGRYCG 1 cut(s) 2437
BshFI GGCC 8 cut(s) 561, 1456, 1700, 2136, 2405, 2440, 2714, 2730
BsiEI CGRYCG 1 cut(s) 2437
BsiHKAI GWGCWC 5 cut(s) 203, 435, 609, 2259, 2469
BsiHKCI CYCGRG 3 cut(s) 1342, 2468, 2930
BslFI GGGAC 7 cut(s) 170, 229, 358, 980, 1635, 2143, 2585
BslI CCNNNNNNNGG 7 cut(s) 64, 290, 863, 1443, 1493, 2721, 2883
BsmAI GTCTC 4 cut(s) 761, 1642, 1780, 2047
BsmFI GGGAC 7 cut(s) 170, 229, 358, 980, 1635, 2143, 2585
BsmI GAATGC 1 cut(s) 1147
BsnI GGCC 8 cut(s) 561, 1456, 1700, 2136, 2405, 2440, 2714, 2730
BsoBI CYCGRG 3 cut(s) 1342, 2468, 2930
Bsp1286I GDGCHC 5 cut(s) 203, 435, 609, 2259, 2469
Bsp13I TCCGGA 1 cut(s) 1053
Bsp68I TCGCGA 1 cut(s) 2304
BspACI CCGC 5 cut(s) 65, 519, 591, 2688, 2912
BspANI GGCC 8 cut(s) 561, 1456, 1700, 2136, 2405, 2440, 2714, 2730
BspCNI CTCAG 8 cut(s) 277, 281, 565, 700, 771, 825, 1585, 2421
BspEI TCCGGA 1 cut(s) 1053
BspFNI CGCG 3 cut(s) 519, 2304, 2688
BspMAI CTGCAG 1 cut(s) 770
BspMI ACCTGC 2 cut(s) 1241, 2068
BspOI GCTAGC 1 cut(s) 1149
BspQI GCTCTTC 1 cut(s) 600
BspTI CTTAAG 2 cut(s) 337, 523
BsrBI CCGCTC 1 cut(s) 593
BsrDI GCAATG 2 cut(s) 1701, 2877
BsrFI RCCGGY 1 cut(s) 2436
BsrI ACTGG 6 cut(s) 1420, 2137, 2202, 2269, 2572, 2616
BssAI RCCGGY 1 cut(s) 2436
BssECI CCNNGG 4 cut(s) 662, 2236, 2715, 2845
BssT1I CCWWGG 2 cut(s) 2236, 2845
Bst2UI CCWGG 5 cut(s) 239, 663, 1864, 2180, 2732
Bst4CI ACNGT 7 cut(s) 989, 1732, 2060, 2155, 2699, 2710, 2975
Bst6I CTCTTC 3 cut(s) 600, 1377, 2822
BstAFI CTTAAG 2 cut(s) 337, 523
BstBAI YACGTR 1 cut(s) 2146
BstC8I GCNNGC 1 cut(s) 1147
BstDSI CCRYGG 1 cut(s) 2715
BstF5I GGATG 7 cut(s) 103, 1264, 2458, 2730, 2941, 2962, 2986
BstFNI CGCG 3 cut(s) 519, 2304, 2688
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 4 cut(s) 283, 519, 672, 2688
BstMAI GTCTC 4 cut(s) 761, 1642, 1780, 2047
BstMCI CGRYCG 1 cut(s) 2437
BstMWI GCNNNNNNNGC 5 cut(s) 107, 250, 430, 2711, 2746
BstNI CCWGG 5 cut(s) 239, 663, 1864, 2180, 2732
BstNSI RCATGY 2 cut(s) 236, 1251
BstSCI CCNGG 8 cut(s) 134, 237, 661, 1862, 2170, 2178, 2491, 2730
BstSFI CTRYAG 6 cut(s) 766, 1944, 1976, 2151, 2230, 2706
BstSNI TACGTA 1 cut(s) 2146
BstUI CGCG 3 cut(s) 519, 2304, 2688
BstV1I GCAGC 3 cut(s) 231, 1694, 2259
BstX2I RGATCY 8 cut(s) 166, 823, 917, 1194, 1970, 2182, 2751, 2892
BstXI CCANNNNNNTGG 1 cut(s) 2623
BstYI RGATCY 8 cut(s) 166, 823, 917, 1194, 1970, 2182, 2751, 2892
BsuI GTATCC 4 cut(s) 218, 795, 2048, 2379
BsuRI GGCC 8 cut(s) 561, 1456, 1700, 2136, 2405, 2440, 2714, 2730
BtgI CCRYGG 1 cut(s) 2715
BtgZI GCGATG 2 cut(s) 2238, 2706
BtsCI GGATG 7 cut(s) 103, 1264, 2458, 2730, 2941, 2962, 2986
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 6 cut(s) 117, 562, 2532, 2623, 2715, 2919
BtuMI TCGCGA 1 cut(s) 2304
BveI ACCTGC 2 cut(s) 1241, 2068
Cac8I GCNNGC 1 cut(s) 1147
CaiI CAGNNNCTG 2 cut(s) 244, 765
CfoI GCGC 4 cut(s) 283, 519, 672, 2688
Cfr10I RCCGGY 1 cut(s) 2436
CpoI CGGWCCG 1 cut(s) 2699
Csp6I GTAC 1 cut(s) 1026
CspI CGGWCCG 1 cut(s) 2699
CviQI GTAC 1 cut(s) 1026
EaeI YGGCCR 3 cut(s) 559, 2438, 2712
Eam1104I CTCTTC 3 cut(s) 600, 1377, 2822
EarI CTCTTC 3 cut(s) 600, 1377, 2822
Ecl136II GAGCTC 3 cut(s) 433, 2257, 2467
Eco105I TACGTA 1 cut(s) 2146
Eco130I CCWWGG 2 cut(s) 2236, 2845
Eco24I GRGCYC 3 cut(s) 435, 2259, 2469
Eco32I GATATC 1 cut(s) 635
Eco47I GGWCC 6 cut(s) 186, 848, 1589, 1622, 2572, 2699
Eco53kI GAGCTC 3 cut(s) 433, 2257, 2467
Eco57I CTGAAG 5 cut(s) 111, 538, 938, 948, 1200
Eco88I CYCGRG 3 cut(s) 1342, 2468, 2930
EcoICRI GAGCTC 3 cut(s) 433, 2257, 2467
EcoO109I RGGNCCY 3 cut(s) 1454, 1622, 2572
EcoRI GAATTC 3 cut(s) 647, 1991, 2636
EcoRII CCWGG 5 cut(s) 237, 661, 1862, 2178, 2730
EcoRV GATATC 1 cut(s) 635
EcoT14I CCWWGG 2 cut(s) 2236, 2845
EcoT22I ATGCAT 2 cut(s) 103, 1473
EcoT38I GRGCYC 3 cut(s) 435, 2259, 2469
ErhI CCWWGG 2 cut(s) 2236, 2845
FaqI GGGAC 7 cut(s) 170, 229, 358, 980, 1635, 2143, 2585
FauI CCCGC 1 cut(s) 598
FauNDI CATATG 3 cut(s) 537, 1045, 1594
Fnu4HI GCNGC 6 cut(s) 245, 520, 1683, 2273, 2689, 2912
FokI GGATG 6 cut(s) 110, 1271, 2465, 2737, 2948, 2969
FriOI GRGCYC 3 cut(s) 435, 2259, 2469
Fsp4HI GCNGC 6 cut(s) 245, 520, 1683, 2273, 2689, 2912
FspBI CTAG 7 cut(s) 59, 1118, 1146, 1395, 1856, 2105, 2992
FspI TGCGCA 1 cut(s) 671
GlaI GCGC 4 cut(s) 282, 518, 671, 2687
GluI GCNGC 6 cut(s) 245, 520, 1683, 2273, 2689, 2912
GsaI CCCAGC 1 cut(s) 1622
GsuI CTGGAG 2 cut(s) 1080, 2273
HaeII RGCGCY 1 cut(s) 284
HaeIII GGCC 8 cut(s) 561, 1456, 1700, 2136, 2405, 2440, 2714, 2730
HhaI GCGC 4 cut(s) 283, 519, 672, 2688
Hin6I GCGC 4 cut(s) 281, 517, 670, 2686
HinP1I GCGC 4 cut(s) 281, 517, 670, 2686
HincII GTYRAC 4 cut(s) 469, 511, 2211, 2794
HindII GTYRAC 4 cut(s) 469, 511, 2211, 2794
HpaI GTTAAC 2 cut(s) 511, 2211
HphI GGTGA 8 cut(s) 218, 1099, 1302, 1994, 2320, 2470, 2505, 2773
Hpy166II GTNNAC 7 cut(s) 469, 511, 1463, 1746, 1836, 2211, 2794
Hpy8I GTNNAC 7 cut(s) 469, 511, 1463, 1746, 1836, 2211, 2794
Hpy99I CGWCG 4 cut(s) 1801, 2821, 2956, 2969
HpyCH4III ACNGT 7 cut(s) 989, 1732, 2060, 2155, 2699, 2710, 2975
HpyCH4IV ACGT 4 cut(s) 374, 1957, 2145, 2595
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 250, 430, 2711, 2746
HpySE526I ACGT 4 cut(s) 374, 1957, 2145, 2595
HspAI GCGC 4 cut(s) 281, 517, 670, 2686
KflI GGGWCCC 1 cut(s) 2572
Kpn2I TCCGGA 1 cut(s) 1053
KspAI GTTAAC 2 cut(s) 511, 2211
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 7 cut(s) 206, 241, 779, 2074, 2254, 2464, 2495
Lsp1109I GCAGC 3 cut(s) 231, 1694, 2259
LweI GCATC 6 cut(s) 88, 1316, 1458, 1557, 1671, 1742
MaeI CTAG 7 cut(s) 59, 1118, 1146, 1395, 1856, 2105, 2992
MaeII ACGT 4 cut(s) 374, 1957, 2145, 2595
MaeIII GTNAC 3 cut(s) 76, 1105, 2840
MbiI CCGCTC 1 cut(s) 593
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 8 cut(s) 166, 823, 917, 1194, 1970, 2182, 2751, 2892
MhlI GDGCHC 5 cut(s) 203, 435, 609, 2259, 2469
MlsI TGGCCA 1 cut(s) 561
MluNI TGGCCA 1 cut(s) 561
MlyI GAGTC 2 cut(s) 2088, 2969
MmeI TCCRAC 5 cut(s) 678, 2430, 2616, 2934, 2988
Mox20I TGGCCA 1 cut(s) 561
Mph1103I ATGCAT 2 cut(s) 103, 1473
MroI TCCGGA 1 cut(s) 1053
MroXI GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2362, 2382
MscI TGGCCA 1 cut(s) 561
MslI CAYNNNNRTG 1 cut(s) 2377
Msp20I TGGCCA 1 cut(s) 561
MspA1I CMGCKG 2 cut(s) 1685, 1943
MspCI CTTAAG 2 cut(s) 337, 523
MspR9I CCNGG 8 cut(s) 136, 239, 663, 1864, 2172, 2180, 2493, 2732
MunI CAATTG 1 cut(s) 888
Mva1269I GAATGC 1 cut(s) 1147
MvaI CCWGG 5 cut(s) 239, 663, 1864, 2180, 2732
MvnI CGCG 3 cut(s) 519, 2304, 2688
MwoI GCNNNNNNNGC 5 cut(s) 107, 250, 430, 2711, 2746
NciI CCSGG 3 cut(s) 136, 2172, 2493
NdeI CATATG 3 cut(s) 537, 1045, 1594
NheI GCTAGC 1 cut(s) 1145
NmuCI GTSAC 2 cut(s) 1105, 2840
NruI TCGCGA 1 cut(s) 2304
NsbI TGCGCA 1 cut(s) 671
NsiI ATGCAT 2 cut(s) 103, 1473
NspI RCATGY 2 cut(s) 236, 1251
PaeR7I CTCGAG 2 cut(s) 2468, 2930
PaqCI CACCTGC 1 cut(s) 2068
PciSI GCTCTTC 1 cut(s) 600
PctI GAATGC 1 cut(s) 1147
PdmI GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2362, 2382
PfeI GAWTC 9 cut(s) 131, 262, 712, 835, 1090, 1205, 2851, 2939, 2948
PflFI GACNNNGTC 1 cut(s) 2789
PflMI CCANNNNNTGG 2 cut(s) 863, 2883
PfoI TCCNGGA 2 cut(s) 134, 237
PkrI GCNGC 6 cut(s) 246, 521, 1684, 2274, 2690, 2913
PleI GAGTC 2 cut(s) 2088, 2968
PpsI GAGTC 2 cut(s) 2088, 2968
Ppu21I YACGTR 1 cut(s) 2146
PpuMI RGGWCCY 2 cut(s) 1622, 2572
PsiI TTATAA 2 cut(s) 1691, 1917
Psp124BI GAGCTC 3 cut(s) 435, 2259, 2469
Psp5II RGGWCCY 2 cut(s) 1622, 2572
Psp6I CCWGG 5 cut(s) 237, 661, 1862, 2178, 2730
PspFI CCCAGC 1 cut(s) 1618
PspGI CCWGG 5 cut(s) 237, 661, 1862, 2178, 2730
PspPPI RGGWCCY 2 cut(s) 1622, 2572
PstI CTGCAG 1 cut(s) 770
PstNI CAGNNNCTG 2 cut(s) 244, 765
PsuI RGATCY 8 cut(s) 166, 823, 917, 1194, 1970, 2182, 2751, 2892
PsyI GACNNNGTC 1 cut(s) 2789
PvuII CAGCTG 2 cut(s) 1685, 1943
RruI TCGCGA 1 cut(s) 2304
RsaI GTAC 1 cut(s) 1027
RsaNI GTAC 1 cut(s) 1026
RseI CAYNNNNRTG 1 cut(s) 2377
Rsr2I CGGWCCG 1 cut(s) 2699
RsrII CGGWCCG 1 cut(s) 2699
SacI GAGCTC 3 cut(s) 435, 2259, 2469
SapI GCTCTTC 1 cut(s) 600
SatI GCNGC 6 cut(s) 245, 520, 1683, 2273, 2689, 2912
SchI GAGTC 2 cut(s) 2088, 2969
ScrFI CCNGG 8 cut(s) 136, 239, 663, 1864, 2172, 2180, 2493, 2732
SduI GDGCHC 5 cut(s) 203, 435, 609, 2259, 2469
SfaNI GCATC 6 cut(s) 88, 1316, 1458, 1557, 1671, 1742
SfcI CTRYAG 6 cut(s) 766, 1944, 1976, 2151, 2230, 2706
Sfr274I CTCGAG 2 cut(s) 2468, 2930
SinI GGWCC 6 cut(s) 186, 848, 1589, 1622, 2572, 2699
SlaI CTCGAG 2 cut(s) 2468, 2930
SmiMI CAYNNNNRTG 1 cut(s) 2377
SmlI CTYRAG 5 cut(s) 337, 523, 1111, 2468, 2930
SmoI CTYRAG 5 cut(s) 337, 523, 1111, 2468, 2930
SnaBI TACGTA 1 cut(s) 2146
SpeI ACTAGT 1 cut(s) 1394
SsiI CCGC 5 cut(s) 65, 519, 591, 2688, 2912
SspMI CTAG 7 cut(s) 59, 1118, 1146, 1395, 1856, 2105, 2992
SstI GAGCTC 3 cut(s) 435, 2259, 2469
StyD4I CCNGG 8 cut(s) 134, 237, 661, 1862, 2170, 2178, 2491, 2730
StyI CCWWGG 2 cut(s) 2236, 2845
TaaI ACNGT 7 cut(s) 989, 1732, 2060, 2155, 2699, 2710, 2975
TaiI ACGT 4 cut(s) 377, 1960, 2148, 2598
TaqI TCGA 9 cut(s) 684, 969, 1605, 1799, 2032, 2469, 2558, 2640, 2931
TaqII GACCGA 1 cut(s) 2015
TatI WGTACW 1 cut(s) 1025
TauI GCSGC 3 cut(s) 522, 2691, 2914
TfiI GAWTC 9 cut(s) 131, 262, 712, 835, 1090, 1205, 2851, 2939, 2948
TscAI CASTG 6 cut(s) 117, 562, 2539, 2623, 2715, 2926
TseFI GTSAC 2 cut(s) 1105, 2840
TseI GCWGC 3 cut(s) 244, 1682, 2272
Tsp45I GTSAC 2 cut(s) 1105, 2840
TspGWI ACGGA 1 cut(s) 930
TspRI CASTG 6 cut(s) 117, 562, 2539, 2623, 2715, 2926
Tth111I GACNNNGTC 1 cut(s) 2789
Van91I CCANNNNNTGG 2 cut(s) 863, 2883
Vha464I CTTAAG 2 cut(s) 337, 523
VpaK11BI GGWCC 6 cut(s) 186, 848, 1589, 1622, 2572, 2699
XapI RAATTY 6 cut(s) 564, 647, 1213, 1565, 1991, 2636
XceI RCATGY 2 cut(s) 236, 1251
XhoI CTCGAG 2 cut(s) 2468, 2930
XmnI GAANNNNTTC 6 cut(s) 733, 1275, 1481, 1580, 2362, 2382
XspI CTAG 7 cut(s) 59, 1118, 1146, 1395, 1856, 2105, 2992
Zsp2I ATGCAT 2 cut(s) 103, 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.