Rmu_sc0000317.1_g000011

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000317.1
OG0002270
Physical Location & Seq
Reverse (-)
71689 .. 75246
3558 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 969 bp
atggccgaggacggcagttcaatagttgcagagttggaccgagcggcagagggattgtcttccagagaagcggagctgaggcaagagattgagcaacacaagcacgaaaaggcagtgctggaggagaagcaccaattcgagattctagagaggctgaaggtgaaggacgagctccggagccaagctgctgataatggagcagttcccggagactgctgcctctatcgtcttcttcaaggtcgactccgacgtcatgttcctcgacgaccgcgacttcctcaaaaccgacgccgagatcggcgacgacggcaagctccacgtcaaggtgtggaagtccaacgcgtccaaacggatttctataatttcctattggattcaggaatggacccagtgtcttctgtgacaaccactgaaatgaatgttgcactaaaaaaatatacaagatatctaaattccttgaggaggccttcattagcaccaggtggtaatggagatcttgacacgttgatgaatggtgtcattaaaaggaagcttaagattattccaaacaatgtgacttggggaatgcagtctgacaatgtctttgaagctatgatcggagacttcatgagacccagaattgatgaggttgatcaactccttgccaaaggggtcaacgtcacagtatacaatggtcaagttgatctcatatgcgcaaccaaggggactgaagcatgggttgaaaagctcaagtgggatggactccaacaatttctgaacaagagtagaacccctctctactgtgggaatgatagatttaccggaggatttaccaggtcgtacaaaaaccttcaattctattggattcttggagcaggacactttgtacctgtggatcagccctgcattgctttgaatatggtggctgatattacacagtcacctgctgctgctgcttctgcttctgcttctccacatagaagaacataa

Protein Analysis

322

Amino Acids

36.5

Weight (kDa)

9.36

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 169 - 304 1.8e-28 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 931
AasI GACNNNNNNGTC 1 cut(s) 249
AatII GACGTC 1 cut(s) 253
Acc16I TGCGCA 1 cut(s) 694
Acc36I ACCTGC 1 cut(s) 931
AccBSI CCGCTC 1 cut(s) 44
AccI GTMKAC 2 cut(s) 241, 666
AccII CGCG 2 cut(s) 271, 342
AccIII TCCGGA 1 cut(s) 174
AciI CCGC 3 cut(s) 44, 71, 269
AclWI GGATC 1 cut(s) 882
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 451
AcuI CTGAAG 2 cut(s) 176, 729
AcyI GRCGYC 2 cut(s) 250, 289
AdeI CACNNNGTG 1 cut(s) 482
AfaI GTAC 2 cut(s) 821, 867
AfiI CCNNNNNNNGG 2 cut(s) 323, 462
AflII CTTAAG 1 cut(s) 533
AflIII ACRYGT 2 cut(s) 340, 501
AgsI TTSAA 6 cut(s) 21, 236, 587, 722, 833, 895
AhdI GACNNNNNGTC 1 cut(s) 391
AjiI CACGTC 1 cut(s) 320
AjnI CCWGG 2 cut(s) 478, 812
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 7 cut(s) 76, 172, 185, 314, 532, 590, 727
AluI AGCT 7 cut(s) 76, 172, 185, 314, 532, 590, 727
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 3 cut(s) 204, 594, 604
AlwI GGATC 1 cut(s) 882
Aor13HI TCCGGA 1 cut(s) 174
AoxI GGCC 2 cut(s) 3, 464
ApeKI GCWGC 5 cut(s) 185, 216, 926, 929, 932
ApoI RAATTY 1 cut(s) 451
ArsI GACNNNNNNTTYG 2 cut(s) 566, 598
AspLEI GCGC 1 cut(s) 695
AspS9I GGNCC 2 cut(s) 37, 385
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 2 cut(s) 172, 912
AvaII GGWCC 2 cut(s) 37, 385
BanII GRGCYC 1 cut(s) 174
BbsI GAAGAC 3 cut(s) 51, 221, 387
Bbv12I GWGCWC 1 cut(s) 174
BbvCI CCTCAGC 1 cut(s) 77
BbvI GCAGC 5 cut(s) 172, 203, 913, 916, 919
BccI CCATC 1 cut(s) 731
BceAI ACGGC 2 cut(s) 28, 323
BciT130I CCWGG 2 cut(s) 480, 814
BclI TGATCA 1 cut(s) 631
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 3 cut(s) 204, 594, 604
BfaI CTAG 1 cut(s) 146
BfrI CTTAAG 1 cut(s) 533
BfuAI ACCTGC 1 cut(s) 931
BglII AGATCT 1 cut(s) 493
BisI GCNGC 6 cut(s) 45, 186, 217, 927, 930, 933
BlsI GCNGC 6 cut(s) 46, 187, 218, 928, 931, 934
Bme1390I CCNGG 3 cut(s) 207, 480, 814
Bme18I GGWCC 2 cut(s) 37, 385
BmeRI GACNNNNNGTC 1 cut(s) 391
BmgBI CACGTC 1 cut(s) 320
BmgT120I GGNCC 2 cut(s) 37, 385
BmiI GGNNCC 2 cut(s) 179, 387
BmrFI CCNGG 3 cut(s) 207, 480, 814
BmrI ACTGGG 1 cut(s) 383
BmuI ACTGGG 1 cut(s) 383
BpiI GAAGAC 3 cut(s) 51, 221, 387
BpmI CTGGAG 1 cut(s) 140
Bpu10I CCTNAGC 1 cut(s) 77
BpuEI CTTGAG 2 cut(s) 478, 713
BpuMI CCSGG 1 cut(s) 207
BsaHI GRCGYC 2 cut(s) 250, 289
BsaI GGTCTC 1 cut(s) 604
BsaJI CCNNGG 2 cut(s) 6, 699
BsaWI WCCGGW 2 cut(s) 174, 800
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 462
Bse1I ACTGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 885
BseAI TCCGGA 1 cut(s) 174
BseBI CCWGG 2 cut(s) 480, 814
BseDI CCNNGG 2 cut(s) 6, 699
BseGI GGATG 1 cut(s) 742
BseLI CCNNNNNNNGG 2 cut(s) 323, 462
BseMI GCAATG 1 cut(s) 885
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 1 cut(s) 389
BseRI GAGGAG 2 cut(s) 137, 475
BseXI GCAGC 5 cut(s) 172, 203, 913, 916, 919
Bsh1236I CGCG 2 cut(s) 271, 342
Bsh1285I CGRYCG 1 cut(s) 269
BshFI GGCC 2 cut(s) 5, 466
BsiEI CGRYCG 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 174
BsiSI CCGG 3 cut(s) 175, 207, 801
BslFI GGGAC 1 cut(s) 718
BslI CCNNNNNNNGG 2 cut(s) 323, 462
BsmAI GTCTC 3 cut(s) 204, 594, 604
BsmFI GGGAC 1 cut(s) 718
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 2 cut(s) 5, 466
Bso31I GGTCTC 1 cut(s) 604
Bsp1286I GDGCHC 1 cut(s) 174
Bsp13I TCCGGA 1 cut(s) 174
Bsp143I GATC 6 cut(s) 295, 493, 594, 631, 682, 874
BspACI CCGC 3 cut(s) 44, 71, 269
BspANI GGCC 2 cut(s) 5, 466
BspCNI CTCAG 1 cut(s) 69
BspEI TCCGGA 1 cut(s) 174
BspFNI CGCG 2 cut(s) 271, 342
BspHI TCATGA 1 cut(s) 606
BspLI GGNNCC 2 cut(s) 179, 387
BspMI ACCTGC 1 cut(s) 931
BspPI GGATC 1 cut(s) 882
BspTI CTTAAG 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 604
BsrBI CCGCTC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 885
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 2 cut(s) 6, 699
BssMI GATC 6 cut(s) 295, 493, 594, 631, 682, 874
BssNAI GTATAC 1 cut(s) 667
BssNI GRCGYC 2 cut(s) 250, 289
BssT1I CCWWGG 1 cut(s) 699
Bst1107I GTATAC 1 cut(s) 667
Bst2UI CCWGG 2 cut(s) 480, 814
Bst4CI ACNGT 3 cut(s) 664, 782, 918
BstACI GRCGYC 2 cut(s) 250, 289
BstAFI CTTAAG 1 cut(s) 533
BstC8I GCNNGC 1 cut(s) 312
BstDEI CTNAG 1 cut(s) 77
BstENI CCTNNNNNAGG 1 cut(s) 460
BstF5I GGATG 1 cut(s) 742
BstFNI CGCG 2 cut(s) 271, 342
BstHHI GCGC 1 cut(s) 695
BstKTI GATC 6 cut(s) 298, 496, 597, 634, 685, 877
BstMAI GTCTC 3 cut(s) 204, 594, 604
BstMBI GATC 6 cut(s) 295, 493, 594, 631, 682, 874
BstMCI CGRYCG 1 cut(s) 269
BstMWI GCNNNNNNNGC 4 cut(s) 100, 307, 932, 938
BstNI CCWGG 2 cut(s) 480, 814
BstSCI CCNGG 3 cut(s) 205, 478, 812
BstUI CGCG 2 cut(s) 271, 342
BstV1I GCAGC 5 cut(s) 172, 203, 913, 916, 919
BstV2I GAAGAC 3 cut(s) 51, 221, 387
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BstZ17I GTATAC 1 cut(s) 667
BsuRI GGCC 2 cut(s) 5, 466
BtrI CACGTC 1 cut(s) 320
BtsCI GGATG 1 cut(s) 742
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 3 cut(s) 120, 396, 408
BveI ACCTGC 1 cut(s) 931
Cac8I GCNNGC 1 cut(s) 312
CciI TCATGA 1 cut(s) 606
CfoI GCGC 1 cut(s) 695
Cfr13I GGNCC 2 cut(s) 37, 385
CseI GACGC 2 cut(s) 297, 331
CsiI ACCWGGT 2 cut(s) 478, 812
Csp6I GTAC 2 cut(s) 820, 866
CviAII CATG 3 cut(s) 254, 607, 714
CviQI GTAC 2 cut(s) 820, 866
DdeI CTNAG 1 cut(s) 77
DpnI GATC 6 cut(s) 297, 495, 596, 633, 684, 876
DpnII GATC 6 cut(s) 295, 493, 594, 631, 682, 874
DraIII CACNNNGTG 1 cut(s) 482
DrdI GACNNNNNNGTC 1 cut(s) 249
DriI GACNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 249
EaeI YGGCCR 1 cut(s) 3
Eam1105I GACNNNNNGTC 1 cut(s) 391
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 699
Eco147I AGGCCT 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 604
Eco32I GATATC 1 cut(s) 446
Eco47I GGWCC 2 cut(s) 37, 385
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 2 cut(s) 176, 729
EcoICRI GAGCTC 1 cut(s) 172
EcoNI CCTNNNNNAGG 1 cut(s) 460
EcoRII CCWGG 2 cut(s) 478, 812
EcoRV GATATC 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 699
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 699
FaeI CATG 3 cut(s) 257, 610, 717
FaqI GGGAC 1 cut(s) 718
FatI CATG 3 cut(s) 253, 606, 713
FauNDI CATATG 1 cut(s) 689
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 2 cut(s) 241, 666
Fnu4HI GCNGC 6 cut(s) 45, 186, 217, 927, 930, 933
FokI GGATG 1 cut(s) 749
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 6 cut(s) 45, 186, 217, 927, 930, 933
FspBI CTAG 1 cut(s) 146
FspI TGCGCA 1 cut(s) 694
GlaI GCGC 1 cut(s) 694
GluI GCNGC 6 cut(s) 45, 186, 217, 927, 930, 933
GsuI CTGGAG 1 cut(s) 140
HaeIII GGCC 2 cut(s) 5, 466
HapII CCGG 3 cut(s) 175, 207, 801
HgaI GACGC 2 cut(s) 297, 331
HhaI GCGC 1 cut(s) 695
Hin1I GRCGYC 2 cut(s) 250, 289
Hin1II CATG 3 cut(s) 257, 610, 717
Hin6I GCGC 1 cut(s) 693
HinP1I GCGC 1 cut(s) 693
HincII GTYRAC 2 cut(s) 242, 655
HindII GTYRAC 2 cut(s) 242, 655
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 5 cut(s) 142, 243, 374, 741, 844
HpaII CCGG 3 cut(s) 175, 207, 801
HphI GGTGA 2 cut(s) 172, 912
Hpy166II GTNNAC 3 cut(s) 242, 655, 667
Hpy188I TCNGA 4 cut(s) 248, 574, 599, 756
Hpy188III TCNNGA 7 cut(s) 63, 139, 146, 175, 378, 497, 607
Hpy8I GTNNAC 3 cut(s) 242, 655, 667
Hpy99I CGWCG 5 cut(s) 252, 267, 291, 306, 309
HpyAV CCTTC 4 cut(s) 151, 157, 477, 839
HpyCH4III ACNGT 3 cut(s) 664, 782, 918
HpyCH4IV ACGT 4 cut(s) 250, 319, 503, 657
HpyCH4V TGCA 4 cut(s) 29, 425, 568, 885
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 307, 932, 938
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 4 cut(s) 250, 319, 503, 657
Hsp92I GRCGYC 2 cut(s) 250, 289
Hsp92II CATG 3 cut(s) 257, 610, 717
HspAI GCGC 1 cut(s) 693
Kpn2I TCCGGA 1 cut(s) 174
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 6 cut(s) 295, 493, 594, 631, 682, 874
LmnI GCTCC 6 cut(s) 73, 177, 177, 197, 319, 851
Lsp1109I GCAGC 5 cut(s) 172, 203, 913, 916, 919
MabI ACCWGGT 2 cut(s) 478, 812
MaeI CTAG 1 cut(s) 146
MaeII ACGT 4 cut(s) 250, 319, 503, 657
MaeIII GTNAC 4 cut(s) 400, 553, 658, 918
MalI GATC 6 cut(s) 297, 495, 596, 633, 684, 876
MbiI CCGCTC 1 cut(s) 44
MboI GATC 6 cut(s) 295, 493, 594, 631, 682, 874
MboII GAAGA 4 cut(s) 51, 221, 224, 387
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 6 cut(s) 134, 361, 451, 618, 749, 833
MluI ACGCGT 1 cut(s) 340
MlyI GAGTC 2 cut(s) 237, 735
MmeI TCCRAC 4 cut(s) 15, 271, 361, 769
MroI TCCGGA 1 cut(s) 174
MseI TTAA 2 cut(s) 522, 534
MslI CAYNNNNRTG 2 cut(s) 413, 506
MspCI CTTAAG 1 cut(s) 533
MspI CCGG 3 cut(s) 175, 207, 801
MspR9I CCNGG 3 cut(s) 207, 480, 814
Mva1269I GAATGC 1 cut(s) 570
MvaI CCWGG 2 cut(s) 480, 814
MvnI CGCG 2 cut(s) 271, 342
MwoI GCNNNNNNNGC 4 cut(s) 100, 307, 932, 938
NciI CCSGG 1 cut(s) 207
NdeI CATATG 1 cut(s) 689
NdeII GATC 6 cut(s) 295, 493, 594, 631, 682, 874
NlaIII CATG 3 cut(s) 257, 610, 717
NlaIV GGNNCC 2 cut(s) 179, 387
NmeAIII GCCGAG 2 cut(s) 31, 317
NmuCI GTSAC 4 cut(s) 400, 553, 658, 918
NsbI TGCGCA 1 cut(s) 694
PagI TCATGA 1 cut(s) 606
PaqCI CACCTGC 1 cut(s) 931
PceI AGGCCT 1 cut(s) 466
PcsI WCGNNNNNNNCGW 2 cut(s) 247, 268
PctI GAATGC 1 cut(s) 570
PfeI GAWTC 3 cut(s) 142, 374, 844
PflFI GACNNNGTC 1 cut(s) 578
PfoI TCCNGGA 1 cut(s) 205
PkrI GCNGC 6 cut(s) 46, 187, 218, 928, 931, 934
PleI GAGTC 2 cut(s) 237, 735
PpsI GAGTC 2 cut(s) 237, 735
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 478, 812
PspGI CCWGG 2 cut(s) 478, 812
PspN4I GGNNCC 2 cut(s) 179, 387
PspPI GGNCC 2 cut(s) 37, 385
PsuI RGATCY 1 cut(s) 493
PsyI GACNNNGTC 1 cut(s) 578
RsaI GTAC 2 cut(s) 821, 867
RsaNI GTAC 2 cut(s) 820, 866
RseI CAYNNNNRTG 2 cut(s) 413, 506
SacI GAGCTC 1 cut(s) 174
SalI GTCGAC 1 cut(s) 240
SaqAI TTAA 2 cut(s) 522, 534
SatI GCNGC 6 cut(s) 45, 186, 217, 927, 930, 933
Sau3AI GATC 6 cut(s) 295, 493, 594, 631, 682, 874
Sau96I GGNCC 2 cut(s) 37, 385
SchI GAGTC 2 cut(s) 237, 735
ScrFI CCNGG 3 cut(s) 207, 480, 814
SduI GDGCHC 1 cut(s) 174
SexAI ACCWGGT 2 cut(s) 478, 812
SinI GGWCC 2 cut(s) 37, 385
SmiMI CAYNNNNRTG 2 cut(s) 413, 506
SmlI CTYRAG 3 cut(s) 457, 533, 728
SmoI CTYRAG 3 cut(s) 457, 533, 728
Sse9I AATT 6 cut(s) 134, 361, 451, 618, 749, 833
SseBI AGGCCT 1 cut(s) 466
SsiI CCGC 3 cut(s) 44, 71, 269
SspMI CTAG 1 cut(s) 146
SstI GAGCTC 1 cut(s) 174
StuI AGGCCT 1 cut(s) 466
StyD4I CCNGG 3 cut(s) 205, 478, 812
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 3 cut(s) 664, 782, 918
TaiI ACGT 4 cut(s) 253, 322, 506, 660
TaqI TCGA 3 cut(s) 138, 241, 262
TaqII GACCGA 1 cut(s) 54
TasI AATT 6 cut(s) 134, 361, 451, 618, 749, 833
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 3 cut(s) 142, 374, 844
Tru1I TTAA 2 cut(s) 522, 534
Tru9I TTAA 2 cut(s) 522, 534
TscAI CASTG 3 cut(s) 120, 396, 415
TseFI GTSAC 4 cut(s) 400, 553, 658, 918
TseI GCWGC 5 cut(s) 185, 216, 926, 929, 932
Tsp45I GTSAC 4 cut(s) 400, 553, 658, 918
TspDTI ATGAA 4 cut(s) 431, 459, 524, 595
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 3 cut(s) 120, 396, 415
Tth111I GACNNNGTC 1 cut(s) 578
Vha464I CTTAAG 1 cut(s) 533
VpaK11BI GGWCC 2 cut(s) 37, 385
XagI CCTNNNNNAGG 1 cut(s) 460
XapI RAATTY 1 cut(s) 451
XbaI TCTAGA 1 cut(s) 145
XmiI GTMKAC 2 cut(s) 241, 666
XspI CTAG 1 cut(s) 146
ZraI GACGTC 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.