Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000577.1

Orthogroup ID

OG0006940

Physical Location & Seq

Genomic Coordinates Reverse (-)

49520 .. 51006

Gene Structure 1487 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1410 bp

atggcataccatggctacgatcccggggatcgtaacaactggtatcagagcccgcacctgggcctgtctccggtgtgtccggcggtgtacagcagtgggatctacggcggttgttacaacacgcatacttctcacccttactgtagccctagctacccaccacctccttcccaattcccattgcaaccaagctaccctccaccaccaccaccttcccaaaccatcatcaatggccgcttactccctcatcaatttccatcaacccacacttaccattcacctcgttttgacccaattccctggatatacccatcgatttcacaacgccattcctatgacccatcagttaccagtgttcattctcagttcatcttcaccaaaaccaggaatccaccacctatttaccattctgctccttcatttccaacctttcagtcacccttaactccacttttgtgccccaactccaatttactgatctcaaacctcattctctccatagcaaaccgttttggggtttcaatcgtagagtcgccatgggtgcaaaagctagagttgccaaaaagacttgaaacccagatctgctccgtgatgtgggtaagtactcgaggtctaagagttgagattatgaaggagattcttcagcaaattgtgggtctaaaacctccattcgatatatcaaaatcgacggcggtgcaattcagaagtctgccgtctttcggaaacacctcgtcgtcttcagtttcagcaacggatgatacataccactctactcccacacaagccccaattacttcacctaaagttgcacatcaagtgtttgatgaaaagccggcgatggagaccctagcaccaactacagaagatgaggagattatcaaggttgtggttgccggaaccattccttcatactcagtccagtcaaagctcggcgattgcaagagggctgcttctattaggtgcaagtccttagagggatttgattacgggtcaaatttgggtcaatgctgtgaaatgccagttgggtctgtcaccgttcctgttgggattgttgggcctatcatttttgatggaagagagttgtcaggccctatggccaccactgaaggttgccatatggaggacctcgctaagagagcagctgagttcaagttcttcatgaaagaccctgacaattatgaaaccagtttccatctcgaaaaggcaaaaaaggggccaaaaacacatgaaattgggcaccaagcatccattatgtcggttagtggcattgagaatgccaaagaagaggaccctacatctattcttggagttaagaattcatcaaagattccctccctagccattgacgcagacaaagaagcctgtaaccatcatcccttgagaaaagttgtgatgacagtggaggattga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004420 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0006720 GO:0006721 GO:0006722 GO:0008150 GO:0008152 GO:0008202 GO:0008299 GO:0008610 GO:0009058 GO:0009526 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016104 GO:0016114 GO:0016125 GO:0016126 GO:0016491 GO:0016614 GO:0016616 GO:0031090 GO:0031903 GO:0031966 GO:0031967 GO:0031975 GO:0031984 GO:0042170 GO:0042175 GO:0042282 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044432 GO:0044435 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0098588 GO:0098805 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00900 ko01100 ko01110 ko01130 ko04152 ko04976 map00900 map01100 map01110 map01130 map04152 map04976

Pfam Domains

Protein Families

HMG-CoA_red

Protein Analysis

469

Amino Acids

51.58

Weight (kDa)

6.52

Isoelectric Point (pI)

52.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMG-CoA_red	PF00368	325 - 371	6.6e-10	Hydroxymethylglutaryl-coenzyme A reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006940)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g17061
malus_domestica	MD02G1071700.v1.1 MD08G1183300.v1.1 MD10G1116300.v1.1
pyrus_communis	pycom02g05550 pycom02g25640
rosa_chinensis	RchiOBHm_Chr3g0463691 RchiOBHm_Chr3g0488461 RchiOBHm_Chr4g0387091 RchiOBHm_Chr4g0393421 RchiOBHm_Chr5g0070501 RchiOBHm_Chr7g0200661
rosa_multiflora	Rmu_co8395177.1_g000001 Rmu_sc0000276.1_g000009 Rmu_sc0000577.1_g000015 Rmu_sc0004908.1_g000012 Rmu_sc0008393.1_g000001 Rmu_sc0009422.1_g000003 Rmu_sc0013203.1_g000003 Rmu_sc0018791.1_g000001 Rmu_sc0040893.1_g000001
rosa_roxburghii	Rroxscaffold_2G00141110 Rroxscaffold_5G00365540 Rroxscaffold_6G00404100 Rroxscaffold_7G00190480 Rroxscaffold_7G00197650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1236
AciI	CCGC	5 cut(s)	53, 83, 108, 235, 692
AclWI	GGATC	3 cut(s)	14, 36, 107
AcoI	YGGCCR	2 cut(s)	232, 1095
AcsI	RAATTY	2 cut(s)	994, 1315
AcuI	CTGAAG	3 cut(s)	626, 723, 1125
AfaI	GTAC	2 cut(s)	89, 604
AfiI	CCNNNNNNNGG	8 cut(s)	58, 59, 70, 384, 514, 594, 719, 1120
AgsI	TTSAA	3 cut(s)	522, 572, 1150
AjnI	CCWGG	3 cut(s)	57, 299, 383
AjuI	GAANNNNNNNTTGG	2 cut(s)	1005, 1037
AleI	CACNNNNGTG	1 cut(s)	454
AluBI	AGCT	5 cut(s)	153, 192, 550, 928, 1142
AluI	AGCT	5 cut(s)	153, 192, 550, 928, 1142
Alw26I	GTCTC	2 cut(s)	72, 836
AlwI	GGATC	3 cut(s)	14, 36, 107
Ama87I	CYCGRG	2 cut(s)	23, 606
AoxI	GGCC	6 cut(s)	61, 232, 1055, 1087, 1095, 1214
ApeKI	GCWGC	2 cut(s)	947, 1139
ApoI	RAATTY	2 cut(s)	994, 1315
ArsI	GACNNNNNNTTYG	2 cut(s)	640, 672
AspS9I	GGNCC	6 cut(s)	61, 1055, 1088, 1123, 1214, 1288
AsuC2I	CCSGG	2 cut(s)	24, 25
AsuHPI	GGTGA	6 cut(s)	125, 270, 367, 429, 789, 1024
AvaI	CYCGRG	2 cut(s)	23, 606
AvaII	GGWCC	2 cut(s)	1123, 1288
BaeGI	GKGCMC	2 cut(s)	461, 1239
BalI	TGGCCA	1 cut(s)	1097
BanI	GGYRCC	1 cut(s)	1236
BanII	GRGCYC	1 cut(s)	53
BbsI	GAAGAC	1 cut(s)	729
BbvI	GCAGC	2 cut(s)	934, 1151
BccI	CCATC	8 cut(s)	230, 265, 319, 349, 832, 1064, 1200, 1377
BceAI	ACGGC	3 cut(s)	121, 697, 705
BcgI	CGANNNNNNTGC	2 cut(s)	676, 710
BciT130I	CCWGG	3 cut(s)	59, 301, 385
BcnI	CCSGG	2 cut(s)	24, 25
BcoDI	GTCTC	2 cut(s)	72, 836
BfaI	CTAG	4 cut(s)	150, 551, 848, 1337
BfmI	CTRYAG	2 cut(s)	142, 858
BglII	AGATCT	1 cut(s)	579
BisI	GCNGC	3 cut(s)	235, 948, 1140
BlsI	GCNGC	3 cut(s)	236, 949, 1141
BmcAI	AGTACT	1 cut(s)	604
Bme1390I	CCNGG	5 cut(s)	24, 25, 59, 301, 385
Bme18I	GGWCC	2 cut(s)	1123, 1288
BmeT110I	CYCGRG	2 cut(s)	23, 606
BmgT120I	GGNCC	6 cut(s)	61, 1055, 1088, 1123, 1214, 1288
BmiI	GGNNCC	4 cut(s)	898, 1215, 1238, 1290
BmrFI	CCNGG	5 cut(s)	24, 25, 59, 301, 385
BmsI	GCATC	1 cut(s)	1253
BpiI	GAAGAC	1 cut(s)	729
BpuEI	CTTGAG	1 cut(s)	1399
BpuMI	CCSGG	2 cut(s)	24, 25
Bsa29I	ATCGAT	1 cut(s)	314
BsaI	GGTCTC	1 cut(s)	836
BsaJI	CCNNGG	6 cut(s)	10, 23, 24, 58, 299, 536
BsaWI	WCCGGW	1 cut(s)	70
Bsc4I	CCNNNNNNNGG	8 cut(s)	58, 59, 70, 384, 514, 594, 719, 1120
Bse118I	RCCGGY	1 cut(s)	832
Bse1I	ACTGG	5 cut(s)	44, 351, 919, 1019, 1185
Bse3DI	GCAATG	1 cut(s)	179
BseBI	CCWGG	3 cut(s)	59, 301, 385
BseCI	ATCGAT	1 cut(s)	314
BseDI	CCNNGG	6 cut(s)	10, 23, 24, 58, 299, 536
BseGI	GGATG	3 cut(s)	760, 1244, 1372
BseLI	CCNNNNNNNGG	8 cut(s)	58, 59, 70, 384, 514, 594, 719, 1120
BseMI	GCAATG	1 cut(s)	179
BseMII	CTCAG	3 cut(s)	377, 927, 1134
BseNI	ACTGG	5 cut(s)	44, 351, 919, 1019, 1185
BseRI	GAGGAG	1 cut(s)	884
BseSI	GKGCMC	2 cut(s)	461, 1239
BseXI	GCAGC	2 cut(s)	934, 1151
BshFI	GGCC	6 cut(s)	63, 234, 1057, 1089, 1097, 1216
BshNI	GGYRCC	1 cut(s)	1236
BshVI	ATCGAT	1 cut(s)	314
BsiHKCI	CYCGRG	2 cut(s)	23, 606
BsiSI	CCGG	5 cut(s)	24, 71, 80, 833, 894
BslI	CCNNNNNNNGG	8 cut(s)	58, 59, 70, 384, 514, 594, 719, 1120
BsmAI	GTCTC	2 cut(s)	72, 836
BsmI	GAATGC	1 cut(s)	1279
BsnI	GGCC	6 cut(s)	63, 234, 1057, 1089, 1097, 1216
Bso31I	GGTCTC	1 cut(s)	836
BsoBI	CYCGRG	2 cut(s)	23, 606
Bsp1286I	GDGCHC	3 cut(s)	53, 461, 1239
Bsp1407I	TGTACA	1 cut(s)	87
Bsp143I	GATC	5 cut(s)	19, 28, 99, 477, 579
Bsp19I	CCATGG	2 cut(s)	10, 536
BspACI	CCGC	5 cut(s)	53, 83, 108, 235, 692
BspANI	GGCC	6 cut(s)	63, 234, 1057, 1089, 1097, 1216
BspCNI	CTCAG	3 cut(s)	376, 926, 1135
BspDI	ATCGAT	1 cut(s)	314
BspHI	TCATGA	1 cut(s)	1158
BspLI	GGNNCC	4 cut(s)	898, 1215, 1238, 1290
BspPI	GGATC	3 cut(s)	14, 36, 107
BspT107I	GGYRCC	1 cut(s)	1236
BspTNI	GGTCTC	1 cut(s)	836
BsrDI	GCAATG	1 cut(s)	179
BsrFI	RCCGGY	1 cut(s)	832
BsrGI	TGTACA	1 cut(s)	87
BsrI	ACTGG	5 cut(s)	44, 351, 919, 1019, 1185
BssAI	RCCGGY	1 cut(s)	832
BssECI	CCNNGG	6 cut(s)	10, 23, 24, 58, 299, 536
BssMI	GATC	5 cut(s)	19, 28, 99, 477, 579
BssT1I	CCWWGG	2 cut(s)	10, 536
Bst2UI	CCWGG	3 cut(s)	59, 301, 385
Bst4CI	ACNGT	4 cut(s)	143, 509, 1036, 1399
Bst6I	CTCTTC	2 cut(s)	1069, 1278
BstAUI	TGTACA	1 cut(s)	87
BstC8I	GCNNGC	2 cut(s)	53, 834
BstDEI	CTNAG	6 cut(s)	363, 614, 913, 970, 1131, 1143
BstDSI	CCRYGG	2 cut(s)	10, 536
BstF5I	GGATG	3 cut(s)	760, 1244, 1372
BstKTI	GATC	5 cut(s)	22, 31, 102, 480, 582
BstMAI	GTCTC	2 cut(s)	72, 836
BstMBI	GATC	5 cut(s)	19, 28, 99, 477, 579
BstMWI	GCNNNNNNNGC	4 cut(s)	541, 556, 1136, 1346
BstNI	CCWGG	3 cut(s)	59, 301, 385
BstSCI	CCNGG	5 cut(s)	22, 23, 57, 299, 383
BstSFI	CTRYAG	2 cut(s)	142, 858
BstSLI	GKGCMC	2 cut(s)	461, 1239
BstV1I	GCAGC	2 cut(s)	934, 1151
BstV2I	GAAGAC	1 cut(s)	729
BstX2I	RGATCY	2 cut(s)	99, 579
BstXI	CCANNNNNNTGG	1 cut(s)	300
BstYI	RGATCY	2 cut(s)	99, 579
Bsu15I	ATCGAT	1 cut(s)	314
BsuRI	GGCC	6 cut(s)	63, 234, 1057, 1089, 1097, 1216
BsuTUI	ATCGAT	1 cut(s)	314
BtgI	CCRYGG	2 cut(s)	10, 536
BtgZI	GCGATG	1 cut(s)	851
BtsCI	GGATG	3 cut(s)	760, 1244, 1372
BtsI	GCAGTG	1 cut(s)	100
BtsIMutI	CAGTG	4 cut(s)	100, 358, 1101, 1404
Cac8I	GCNNGC	2 cut(s)	53, 834
CciI	TCATGA	1 cut(s)	1158
Cfr10I	RCCGGY	1 cut(s)	832
Cfr13I	GGNCC	6 cut(s)	61, 1055, 1088, 1123, 1214, 1288
Cfr9I	CCCGGG	1 cut(s)	23
ClaI	ATCGAT	1 cut(s)	314
CseI	GACGC	1 cut(s)	1355
Csp6I	GTAC	2 cut(s)	88, 603
CviAII	CATG	4 cut(s)	11, 537, 1159, 1226
CviQI	GTAC	2 cut(s)	88, 603
DdeI	CTNAG	6 cut(s)	363, 614, 913, 970, 1131, 1143
DpnI	GATC	5 cut(s)	21, 30, 101, 479, 581
DpnII	GATC	5 cut(s)	19, 28, 99, 477, 579
EaeI	YGGCCR	2 cut(s)	232, 1095
Eam1104I	CTCTTC	2 cut(s)	1069, 1278
EarI	CTCTTC	2 cut(s)	1069, 1278
Eco130I	CCWWGG	2 cut(s)	10, 536
Eco24I	GRGCYC	1 cut(s)	53
Eco31I	GGTCTC	1 cut(s)	836
Eco47I	GGWCC	2 cut(s)	1123, 1288
Eco57I	CTGAAG	3 cut(s)	626, 723, 1125
Eco88I	CYCGRG	2 cut(s)	23, 606
EcoO109I	RGGNCCY	3 cut(s)	1088, 1123, 1288
EcoRI	GAATTC	1 cut(s)	1315
EcoRII	CCWGG	3 cut(s)	57, 299, 383
EcoT14I	CCWWGG	2 cut(s)	10, 536
EcoT38I	GRGCYC	1 cut(s)	53
ErhI	CCWWGG	2 cut(s)	10, 536
FaeI	CATG	4 cut(s)	14, 540, 1162, 1229
FatI	CATG	4 cut(s)	10, 536, 1158, 1225
FauI	CCCGC	1 cut(s)	60
FauNDI	CATATG	1 cut(s)	1116
Fnu4HI	GCNGC	3 cut(s)	235, 948, 1140
FokI	GGATG	3 cut(s)	767, 1231, 1359
FriOI	GRGCYC	1 cut(s)	53
Fsp4HI	GCNGC	3 cut(s)	235, 948, 1140
FspBI	CTAG	4 cut(s)	150, 551, 848, 1337
GluI	GCNGC	3 cut(s)	235, 948, 1140
HaeIII	GGCC	6 cut(s)	63, 234, 1057, 1089, 1097, 1216
HapII	CCGG	5 cut(s)	24, 71, 80, 833, 894
HgaI	GACGC	1 cut(s)	1355
Hin1II	CATG	4 cut(s)	14, 540, 1162, 1229
HinfI	GANTC	4 cut(s)	388, 530, 637, 1327
HpaII	CCGG	5 cut(s)	24, 71, 80, 833, 894
HphI	GGTGA	6 cut(s)	125, 270, 367, 429, 789, 1024
Hpy166II	GTNNAC	1 cut(s)	88
Hpy188I	TCNGA	3 cut(s)	48, 704, 722
Hpy188III	TCNNGA	2 cut(s)	1159, 1196
Hpy8I	GTNNAC	1 cut(s)	88
Hpy99I	CGWCG	2 cut(s)	691, 736
HpyAV	CCTTC	6 cut(s)	177, 222, 426, 625, 915, 1100
HpyCH4III	ACNGT	4 cut(s)	143, 509, 1036, 1399
HpyCH4V	TGCA	6 cut(s)	184, 544, 697, 809, 939, 963
HpyF10VI	GCNNNNNNNGC	4 cut(s)	541, 556, 1136, 1346
HpyF3I	CTNAG	6 cut(s)	363, 614, 913, 970, 1131, 1143
Hsp92II	CATG	4 cut(s)	14, 540, 1162, 1229
KroI	GCCGGC	1 cut(s)	832
KroNI	GCCGGC	1 cut(s)	834
Kzo9I	GATC	5 cut(s)	19, 28, 99, 477, 579
LmnI	GCTCC	2 cut(s)	418, 590
Lsp1109I	GCAGC	2 cut(s)	934, 1151
LweI	GCATC	1 cut(s)	1253
MaeI	CTAG	4 cut(s)	150, 551, 848, 1337
MaeIII	GTNAC	6 cut(s)	32, 113, 346, 435, 1030, 1364
MalI	GATC	5 cut(s)	21, 30, 101, 479, 581
MboI	GATC	5 cut(s)	19, 28, 99, 477, 579
MboII	GAAGA	7 cut(s)	364, 632, 729, 875, 1086, 1147, 1295
MflI	RGATCY	2 cut(s)	99, 579
MhlI	GDGCHC	3 cut(s)	53, 461, 1239
MlsI	TGGCCA	1 cut(s)	1097
MluNI	TGGCCA	1 cut(s)	1097
MlyI	GAGTC	1 cut(s)	539
MmeI	TCCRAC	1 cut(s)	449
Mox20I	TGGCCA	1 cut(s)	1097
MroNI	GCCGGC	1 cut(s)	832
MscI	TGGCCA	1 cut(s)	1097
MseI	TTAA	2 cut(s)	443, 1311
MslI	CAYNNNNRTG	2 cut(s)	333, 454
Msp20I	TGGCCA	1 cut(s)	1097
MspA1I	CMGCKG	1 cut(s)	1142
MspI	CCGG	5 cut(s)	24, 71, 80, 833, 894
MspR9I	CCNGG	5 cut(s)	24, 25, 59, 301, 385
Mva1269I	GAATGC	1 cut(s)	1279
MvaI	CCWGG	3 cut(s)	59, 301, 385
MwoI	GCNNNNNNNGC	4 cut(s)	541, 556, 1136, 1346
NaeI	GCCGGC	1 cut(s)	834
NciI	CCSGG	2 cut(s)	24, 25
NcoI	CCATGG	2 cut(s)	10, 536
NdeI	CATATG	1 cut(s)	1116
NdeII	GATC	5 cut(s)	19, 28, 99, 477, 579
NgoMIV	GCCGGC	1 cut(s)	832
NlaIII	CATG	4 cut(s)	14, 540, 1162, 1229
NlaIV	GGNNCC	4 cut(s)	898, 1215, 1238, 1290
NmeAIII	GCCGAG	1 cut(s)	909
NmuCI	GTSAC	2 cut(s)	435, 1030
OliI	CACNNNNGTG	1 cut(s)	454
PaeR7I	CTCGAG	1 cut(s)	606
PagI	TCATGA	1 cut(s)	1158
PctI	GAATGC	1 cut(s)	1279
PdiI	GCCGGC	1 cut(s)	834
PfeI	GAWTC	3 cut(s)	388, 637, 1327
PkrI	GCNGC	3 cut(s)	236, 949, 1141
PleI	GAGTC	1 cut(s)	538
PpsI	GAGTC	1 cut(s)	538
PpuMI	RGGWCCY	2 cut(s)	1123, 1288
Psp5II	RGGWCCY	2 cut(s)	1123, 1288
Psp6I	CCWGG	3 cut(s)	57, 299, 383
PspGI	CCWGG	3 cut(s)	57, 299, 383
PspN4I	GGNNCC	4 cut(s)	898, 1215, 1238, 1290
PspPI	GGNCC	6 cut(s)	61, 1055, 1088, 1123, 1214, 1288
PspPPI	RGGWCCY	2 cut(s)	1123, 1288
PspXI	VCTCGAGB	1 cut(s)	606
PsuI	RGATCY	2 cut(s)	99, 579
PvuII	CAGCTG	1 cut(s)	1142
RsaI	GTAC	2 cut(s)	89, 604
RsaNI	GTAC	2 cut(s)	88, 603
RseI	CAYNNNNRTG	2 cut(s)	333, 454
SaqAI	TTAA	2 cut(s)	443, 1311
SatI	GCNGC	3 cut(s)	235, 948, 1140
Sau3AI	GATC	5 cut(s)	19, 28, 99, 477, 579
Sau96I	GGNCC	6 cut(s)	61, 1055, 1088, 1123, 1214, 1288
ScaI	AGTACT	1 cut(s)	604
SchI	GAGTC	1 cut(s)	539
ScrFI	CCNGG	5 cut(s)	24, 25, 59, 301, 385
SduI	GDGCHC	3 cut(s)	53, 461, 1239
SfaNI	GCATC	1 cut(s)	1253
SfcI	CTRYAG	2 cut(s)	142, 858
Sfr274I	CTCGAG	1 cut(s)	606
SinI	GGWCC	2 cut(s)	1123, 1288
SlaI	CTCGAG	1 cut(s)	606
SmaI	CCCGGG	1 cut(s)	25
SmiMI	CAYNNNNRTG	2 cut(s)	333, 454
SmlI	CTYRAG	2 cut(s)	606, 1378
SmoI	CTYRAG	2 cut(s)	606, 1378
SsiI	CCGC	5 cut(s)	53, 83, 108, 235, 692
SspMI	CTAG	4 cut(s)	150, 551, 848, 1337
StyD4I	CCNGG	5 cut(s)	22, 23, 57, 299, 383
StyI	CCWWGG	2 cut(s)	10, 536
TaaI	ACNGT	4 cut(s)	143, 509, 1036, 1399
TaqI	TCGA	5 cut(s)	314, 607, 672, 686, 1197
TatI	WGTACW	2 cut(s)	87, 602
TauI	GCSGC	1 cut(s)	237
TfiI	GAWTC	3 cut(s)	388, 637, 1327
Tru1I	TTAA	2 cut(s)	443, 1311
Tru9I	TTAA	2 cut(s)	443, 1311
TscAI	CASTG	4 cut(s)	100, 358, 1108, 1404
TseFI	GTSAC	2 cut(s)	435, 1030
TseI	GCWGC	2 cut(s)	947, 1139
Tsp45I	GTSAC	2 cut(s)	435, 1030
TspGWI	ACGGA	2 cut(s)	577, 767
TspMI	CCCGGG	1 cut(s)	23
TspRI	CASTG	4 cut(s)	100, 358, 1108, 1404
VpaK11BI	GGWCC	2 cut(s)	1123, 1288
XapI	RAATTY	2 cut(s)	994, 1315
XhoI	CTCGAG	1 cut(s)	606
XmaI	CCCGGG	1 cut(s)	23
XspI	CTAG	4 cut(s)	150, 551, 848, 1337
ZrmI	AGTACT	1 cut(s)	604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.