Rroxscaffold_5G00365540

Ribosomal protein L6e

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006940
Physical Location & Seq
Forward (+)
47114192 .. 47114990
799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365540.1

Sequence Viewer

Length: 513 bp
ATGACTACACCACCTATTTACCATTCTACTCCATCAATTCCAACCTTTCAATCACCCTCTGTCTCCACTTTTGTGCCCCAACTCCAATTTACGATCCTAAACCTCTTTCTCTTCATAGCAAACCGTTTCGGGGTTTCGGACGTCGAGTCGCCATGGGTGCAAAAGAGAGTTGCCAAAAAGACCCGAAACCCGGATCTGCTCCGTGATGTGGGTAAGTACTCGAGGTCCAAGAGGTACCACAAGCGCGGTCTTTGGGCCATTAAGGCAAACAACGGCTATGTATTCCCACGCCACAATGCCAAGGCGGCGGTTGAGATTATGGAAGAGCTTCTTCGGTTACTTGTGGGATTTGGTGACGAGGACAACGTGGATGAGAAGGACGAGCCCACAAAAGTCATAGTGGATGCACTTGTTGACAACAATGTTCTCGAGCTATTGGTTCGAGAATCGCTCCGATTGTCGATTCCGACGCGGCGAGATTGCCACCATCTACGGTACTCTCGCGACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6e Ribosomal_L6e_N

Protein Analysis

170

Amino Acids

19.6

Weight (kDa)

9.27

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6e_N PF03868 55 - 101 3.2e-09 Ribosomal protein L6, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
Acc65I GGTACC 1 cut(s) 234
AccB1I GGYRCC 1 cut(s) 234
AccII CGCG 3 cut(s) 246, 472, 504
AciI CCGC 4 cut(s) 246, 305, 308, 472
AclWI GGATC 2 cut(s) 88, 201
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 3 cut(s) 218, 236, 497
AfiI CCNNNNNNNGG 3 cut(s) 130, 190, 208
AgsI TTSAA 1 cut(s) 50
AhdI GACNNNNNGTC 1 cut(s) 145
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 2 cut(s) 328, 433
AluI AGCT 2 cut(s) 328, 433
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 2 cut(s) 88, 201
Ama87I CYCGRG 2 cut(s) 220, 428
AoxI GGCC 1 cut(s) 255
Asp700I GAANNNNTTC 1 cut(s) 327
Asp718I GGTACC 1 cut(s) 234
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 2 cut(s) 225, 255
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 2 cut(s) 45, 365
AvaI CYCGRG 2 cut(s) 220, 428
AvaII GGWCC 1 cut(s) 225
BaeGI GKGCMC 1 cut(s) 78
BanI GGYRCC 1 cut(s) 234
BanII GRGCYC 1 cut(s) 387
BccI CCATC 2 cut(s) 40, 495
BceAI ACGGC 1 cut(s) 289
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 67
BglI GCCNNNNNGGC 2 cut(s) 263, 305
BisI GCNGC 2 cut(s) 306, 473
BlsI GCNGC 2 cut(s) 307, 474
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 225
BmeRI GACNNNNNGTC 1 cut(s) 145
BmeT110I CYCGRG 2 cut(s) 220, 428
BmgT120I GGNCC 2 cut(s) 225, 255
BmiI GGNNCC 1 cut(s) 236
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 394
BplI GAGNNNNNCTC 2 cut(s) 435, 467
BpuMI CCSGG 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 2 cut(s) 152, 300
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 190, 208
BseDI CCNNGG 2 cut(s) 152, 300
BseGI GGATG 2 cut(s) 376, 409
BseLI CCNNNNNNNGG 3 cut(s) 130, 190, 208
BseSI GKGCMC 1 cut(s) 78
Bsh1236I CGCG 3 cut(s) 246, 472, 504
BshFI GGCC 1 cut(s) 257
BshNI GGYRCC 1 cut(s) 234
BsiHKCI CYCGRG 2 cut(s) 220, 428
BsiSI CCGG 1 cut(s) 191
BslI CCNNNNNNNGG 3 cut(s) 130, 190, 208
BsmAI GTCTC 1 cut(s) 67
BsnI GGCC 1 cut(s) 257
BsoBI CYCGRG 2 cut(s) 220, 428
Bsp1286I GDGCHC 2 cut(s) 78, 387
Bsp143I GATC 2 cut(s) 93, 193
Bsp19I CCATGG 1 cut(s) 152
Bsp68I TCGCGA 1 cut(s) 504
BspACI CCGC 4 cut(s) 246, 305, 308, 472
BspANI GGCC 1 cut(s) 257
BspFNI CGCG 3 cut(s) 246, 472, 504
BspLI GGNNCC 1 cut(s) 236
BspPI GGATC 2 cut(s) 88, 201
BspQI GCTCTTC 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 234
BssECI CCNNGG 2 cut(s) 152, 300
BssMI GATC 2 cut(s) 93, 193
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 2 cut(s) 152, 300
Bst4CI ACNGT 2 cut(s) 125, 495
Bst6I CTCTTC 2 cut(s) 116, 318
BstACI GRCGYC 1 cut(s) 141
BstDSI CCRYGG 1 cut(s) 152
BstF5I GGATG 2 cut(s) 376, 409
BstFNI CGCG 3 cut(s) 246, 472, 504
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 2 cut(s) 96, 196
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 2 cut(s) 93, 193
BstMWI GCNNNNNNNGC 3 cut(s) 157, 263, 305
BstSCI CCNGG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 3 cut(s) 246, 472, 504
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 152
BtsCI GGATG 2 cut(s) 376, 409
BtuMI TCGCGA 1 cut(s) 504
CfoI GCGC 1 cut(s) 246
Cfr13I GGNCC 2 cut(s) 225, 255
CseI GACGC 1 cut(s) 478
Csp6I GTAC 3 cut(s) 217, 235, 496
CviAII CATG 1 cut(s) 153
CviJI RGCY 5 cut(s) 257, 276, 328, 385, 433
CviKI_1 RGCY 5 cut(s) 257, 276, 328, 385, 433
CviQI GTAC 3 cut(s) 217, 235, 496
DpnI GATC 2 cut(s) 95, 195
DpnII GATC 2 cut(s) 93, 193
DriI GACNNNNNGTC 1 cut(s) 145
Eam1104I CTCTTC 2 cut(s) 116, 318
Eam1105I GACNNNNNGTC 1 cut(s) 145
EarI CTCTTC 2 cut(s) 116, 318
Eco130I CCWWGG 2 cut(s) 152, 300
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 225
Eco88I CYCGRG 2 cut(s) 220, 428
EcoT14I CCWWGG 2 cut(s) 152, 300
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 2 cut(s) 152, 300
FaeI CATG 1 cut(s) 156
FaiI YATR 5 cut(s) 116, 154, 279, 320, 398
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FatI CATG 1 cut(s) 152
Fnu4HI GCNGC 2 cut(s) 306, 473
FokI GGATG 2 cut(s) 383, 416
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 2 cut(s) 306, 473
GlaI GCGC 1 cut(s) 245
GluI GCNGC 2 cut(s) 306, 473
HaeIII GGCC 1 cut(s) 257
HapII CCGG 1 cut(s) 191
HgaI GACGC 1 cut(s) 478
HhaI GCGC 1 cut(s) 246
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 1 cut(s) 156
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 3 cut(s) 146, 446, 463
HpaII CCGG 1 cut(s) 191
HphI GGTGA 2 cut(s) 45, 365
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 3 cut(s) 139, 455, 468
Hpy188III TCNNGA 3 cut(s) 428, 443, 503
Hpy8I GTNNAC 1 cut(s) 415
Hpy99I CGWCG 3 cut(s) 146, 472, 509
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 2 cut(s) 125, 495
HpyCH4IV ACGT 2 cut(s) 141, 366
HpyCH4V TGCA 2 cut(s) 160, 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 263, 305
HpySE526I ACGT 2 cut(s) 141, 366
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 1 cut(s) 156
HspAI GCGC 1 cut(s) 244
KpnI GGTACC 1 cut(s) 238
Kzo9I GATC 2 cut(s) 93, 193
LguI GCTCTTC 1 cut(s) 318
LmnI GCTCC 2 cut(s) 204, 456
LpnPI CCDG 1 cut(s) 204
LweI GCATC 1 cut(s) 394
MaeII ACGT 2 cut(s) 141, 366
MaeIII GTNAC 2 cut(s) 336, 353
MalI GATC 2 cut(s) 95, 195
MboI GATC 2 cut(s) 93, 193
MboII GAAGA 3 cut(s) 103, 323, 335
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 2 cut(s) 78, 387
MluCI AATT 2 cut(s) 36, 86
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 2 cut(s) 65, 491
MnlI CCTC 5 cut(s) 67, 113, 216, 225, 352
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 1 cut(s) 71
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 1 cut(s) 191
MvnI CGCG 3 cut(s) 246, 472, 504
MwoI GCNNNNNNNGC 3 cut(s) 157, 263, 305
NciI CCSGG 1 cut(s) 191
NcoI CCATGG 1 cut(s) 152
NdeII GATC 2 cut(s) 93, 193
NlaIII CATG 1 cut(s) 156
NlaIV GGNNCC 1 cut(s) 236
NmuCI GTSAC 1 cut(s) 353
NruI TCGCGA 1 cut(s) 504
OliI CACNNNNGTG 1 cut(s) 71
PaeR7I CTCGAG 2 cut(s) 220, 428
PciSI GCTCTTC 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 2 cut(s) 446, 463
PkrI GCNGC 2 cut(s) 307, 474
PleI GAGTC 1 cut(s) 154
PpsI GAGTC 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 236
PspPI GGNCC 2 cut(s) 225, 255
PspXI VCTCGAGB 1 cut(s) 220
PsuI RGATCY 1 cut(s) 193
RruI TCGCGA 1 cut(s) 504
RsaI GTAC 3 cut(s) 218, 236, 497
RsaNI GTAC 3 cut(s) 217, 235, 496
RseI CAYNNNNRTG 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 318
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 2 cut(s) 306, 473
Sau3AI GATC 2 cut(s) 93, 193
Sau96I GGNCC 2 cut(s) 225, 255
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 2 cut(s) 78, 387
SetI ASST 9 cut(s) 16, 47, 105, 144, 227, 236, 330, 369, 435
SfaNI GCATC 1 cut(s) 394
Sfr274I CTCGAG 2 cut(s) 220, 428
SinI GGWCC 1 cut(s) 225
SlaI CTCGAG 2 cut(s) 220, 428
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 2 cut(s) 220, 428
SmoI CTYRAG 2 cut(s) 220, 428
Sse9I AATT 2 cut(s) 36, 86
SsiI CCGC 4 cut(s) 246, 305, 308, 472
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 2 cut(s) 152, 300
TaaI ACNGT 2 cut(s) 125, 495
TaiI ACGT 2 cut(s) 144, 369
TaqI TCGA 5 cut(s) 144, 221, 429, 442, 461
TasI AATT 2 cut(s) 36, 86
TatI WGTACW 1 cut(s) 216
TauI GCSGC 2 cut(s) 308, 475
TfiI GAWTC 2 cut(s) 446, 463
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TseFI GTSAC 1 cut(s) 353
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 1 cut(s) 103
TspGWI ACGGA 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 225
XhoI CTCGAG 2 cut(s) 220, 428
XmnI GAANNNNTTC 1 cut(s) 327
ZraI GACGTC 1 cut(s) 142
ZrmI AGTACT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.