Rmu_sc0000704.1_g000008

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000704.1
OG0005531
Physical Location & Seq
Reverse (-)
45589 .. 47889
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
atgaccgttgggggttccaacagtcagcaagggacagaacagatggcgggccccacatctgcagatgaaaaagcagctcaccttctctctccagcgccgtggctcgcgtgcttcttcctcccttcagctggcgcgcgcgtctgcgttactttctcttctcagcgcgttcgttcatcttttgggctgagacagcaagtgggctggtccccatctacagtgaccgagtcctggtcttggaaaagtctcaaatatgagacttcaaatggccccaagtcttatatttataagaccagatccaccaaaaagccatggttggagatgaaaagtcttataattggccaaaatctagtttggcaccccatggttggagttgccattccaagaccaagactcggatactgttcatggggcccaacaaatgggctgttccaatacatccaaggtgccaatttgaattgctctcgaattggcatgacagcccctgtgctgacaagaattgtccctagagctggccctctacactcatccgcatactttgtgagattctatttgcgagtgaagtttcaagcttccccacccacacccctcccggaactcaacttgaaaccttatacatgggacagtcagaaaattctcgggattggcaagagatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

217

Amino Acids

24.08

Weight (kDa)

10.18

Isoelectric Point (pI)

41.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 140 - 211 6.5e-13 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 285, 332
AccB1I GGYRCC 2 cut(s) 354, 443
AccB7I CCANNNNNTGG 1 cut(s) 419
AccII CGCG 5 cut(s) 107, 134, 136, 138, 165
AciI CCGC 2 cut(s) 47, 528
AclWI GGATC 1 cut(s) 288
AcoI YGGCCR 1 cut(s) 337
AcsI RAATTY 1 cut(s) 630
AcuI CTGAAG 1 cut(s) 108
AfiI CCNNNNNNNGG 7 cut(s) 128, 228, 234, 365, 392, 419, 509
AgsI TTSAA 4 cut(s) 261, 454, 566, 604
AjnI CCWGG 1 cut(s) 227
AluBI AGCT 4 cut(s) 77, 128, 509, 569
AluI AGCT 4 cut(s) 77, 128, 509, 569
Alw26I GTCTC 3 cut(s) 181, 248, 248
AlwI GGATC 1 cut(s) 288
AlwNI CAGNNNCTG 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 5 cut(s) 49, 265, 337, 409, 511
ApaI GGGCCC 2 cut(s) 53, 413
ApeKI GCWGC 1 cut(s) 74
ApoI RAATTY 1 cut(s) 630
AspLEI GCGC 5 cut(s) 97, 134, 136, 138, 165
AspS9I GGNCC 7 cut(s) 49, 50, 204, 266, 409, 410, 512
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 1 cut(s) 204
BaeGI GKGCMC 2 cut(s) 53, 413
BalI TGGCCA 1 cut(s) 339
BanI GGYRCC 2 cut(s) 354, 443
BanII GRGCYC 2 cut(s) 53, 413
BbvI GCAGC 1 cut(s) 86
BccI CCATC 2 cut(s) 37, 217
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 229
BciVI GTATCC 1 cut(s) 389
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 3 cut(s) 181, 248, 248
BfaI CTAG 2 cut(s) 347, 504
BfmI CTRYAG 2 cut(s) 60, 213
BfoI RGCGCY 1 cut(s) 98
BfuI GTATCC 1 cut(s) 389
BisI GCNGC 1 cut(s) 75
BlsI GCNGC 1 cut(s) 76
Bme1390I CCNGG 2 cut(s) 229, 590
Bme18I GGWCC 1 cut(s) 204
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 7 cut(s) 49, 50, 204, 266, 409, 410, 512
BmiI GGNNCC 9 cut(s) 16, 51, 52, 206, 268, 356, 410, 411, 445
BmrFI CCNGG 2 cut(s) 229, 590
BpmI CTGGAG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 590
BsaJI CCNNGG 4 cut(s) 98, 308, 360, 439
Bsc4I CCNNNNNNNGG 7 cut(s) 128, 228, 234, 365, 392, 419, 509
BseBI CCWGG 1 cut(s) 229
BseDI CCNNGG 4 cut(s) 98, 308, 360, 439
BseGI GGATG 2 cut(s) 435, 524
BseLI CCNNNNNNNGG 7 cut(s) 128, 228, 234, 365, 392, 419, 509
BseMII CTCAG 2 cut(s) 173, 176
BsePI GCGCGC 2 cut(s) 132, 134
BseSI GKGCMC 2 cut(s) 53, 413
BseXI GCAGC 1 cut(s) 86
Bsh1236I CGCG 5 cut(s) 107, 134, 136, 138, 165
BshFI GGCC 5 cut(s) 51, 267, 339, 411, 513
BshNI GGYRCC 2 cut(s) 354, 443
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 4 cut(s) 46, 190, 485, 632
BslI CCNNNNNNNGG 7 cut(s) 128, 228, 234, 365, 392, 419, 509
BsmAI GTCTC 3 cut(s) 181, 248, 248
BsmFI GGGAC 4 cut(s) 46, 190, 485, 632
BsnI GGCC 5 cut(s) 51, 267, 339, 411, 513
BsoBI CYCGRG 1 cut(s) 635
Bsp120I GGGCCC 2 cut(s) 49, 409
Bsp1286I GDGCHC 2 cut(s) 53, 413
Bsp143I GATC 1 cut(s) 293
Bsp19I CCATGG 2 cut(s) 308, 360
BspACI CCGC 2 cut(s) 47, 528
BspANI GGCC 5 cut(s) 51, 267, 339, 411, 513
BspCNI CTCAG 2 cut(s) 172, 177
BspFNI CGCG 5 cut(s) 107, 134, 136, 138, 165
BspLI GGNNCC 9 cut(s) 16, 51, 52, 206, 268, 356, 410, 411, 445
BspMAI CTGCAG 1 cut(s) 64
BspPI GGATC 1 cut(s) 288
BspT107I GGYRCC 2 cut(s) 354, 443
BssECI CCNNGG 4 cut(s) 98, 308, 360, 439
BssHII GCGCGC 2 cut(s) 132, 134
BssMI GATC 1 cut(s) 293
BssT1I CCWWGG 3 cut(s) 308, 360, 439
Bst2UI CCWGG 1 cut(s) 229
Bst4CI ACNGT 5 cut(s) 7, 23, 217, 401, 623
Bst6I CTCTTC 1 cut(s) 160
BstC8I GCNNGC 7 cut(s) 49, 105, 109, 130, 134, 136, 511
BstDEI CTNAG 2 cut(s) 159, 185
BstDSI CCRYGG 3 cut(s) 98, 308, 360
BstF5I GGATG 2 cut(s) 435, 524
BstFNI CGCG 5 cut(s) 107, 134, 136, 138, 165
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 5 cut(s) 97, 134, 136, 138, 165
BstKTI GATC 1 cut(s) 296
BstMAI GTCTC 3 cut(s) 181, 248, 248
BstMBI GATC 1 cut(s) 293
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstNI CCWGG 1 cut(s) 229
BstSCI CCNGG 2 cut(s) 227, 588
BstSFI CTRYAG 2 cut(s) 60, 213
BstSLI GKGCMC 2 cut(s) 53, 413
BstUI CGCG 5 cut(s) 107, 134, 136, 138, 165
BstV1I GCAGC 1 cut(s) 86
BstX2I RGATCY 1 cut(s) 293
BstXI CCANNNNNNTGG 1 cut(s) 99
BstYI RGATCY 1 cut(s) 293
BsuI GTATCC 1 cut(s) 389
BsuRI GGCC 5 cut(s) 51, 267, 339, 411, 513
BtgI CCRYGG 3 cut(s) 98, 308, 360
BtsCI GGATG 2 cut(s) 435, 524
BtsIMutI CAGTG 1 cut(s) 222
Cac8I GCNNGC 7 cut(s) 49, 105, 109, 130, 134, 136, 511
CaiI CAGNNNCTG 1 cut(s) 482
CfoI GCGC 5 cut(s) 97, 134, 136, 138, 165
Cfr13I GGNCC 7 cut(s) 49, 50, 204, 266, 409, 410, 512
CseI GACGC 1 cut(s) 127
CviAII CATG 5 cut(s) 309, 361, 405, 472, 615
DdeI CTNAG 2 cut(s) 159, 185
DpnI GATC 1 cut(s) 295
DpnII GATC 1 cut(s) 293
EaeI YGGCCR 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 160
Eco130I CCWWGG 3 cut(s) 308, 360, 439
Eco24I GRGCYC 2 cut(s) 53, 413
Eco47I GGWCC 1 cut(s) 204
Eco57I CTGAAG 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 635
EcoO109I RGGNCCY 2 cut(s) 50, 409
EcoRII CCWGG 1 cut(s) 227
EcoT14I CCWWGG 3 cut(s) 308, 360, 439
EcoT38I GRGCYC 2 cut(s) 53, 413
ErhI CCWWGG 3 cut(s) 308, 360, 439
FaeI CATG 5 cut(s) 312, 364, 408, 475, 618
FaqI GGGAC 4 cut(s) 46, 190, 485, 632
FatI CATG 5 cut(s) 308, 360, 404, 471, 614
FauI CCCGC 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 75
FokI GGATG 2 cut(s) 422, 511
FriOI GRGCYC 2 cut(s) 53, 413
Fsp4HI GCNGC 1 cut(s) 75
FspBI CTAG 2 cut(s) 347, 504
GlaI GCGC 5 cut(s) 96, 133, 135, 137, 164
GluI GCNGC 1 cut(s) 75
GsuI CTGGAG 1 cut(s) 75
HaeII RGCGCY 1 cut(s) 98
HaeIII GGCC 5 cut(s) 51, 267, 339, 411, 513
HapII CCGG 1 cut(s) 590
HgaI GACGC 1 cut(s) 127
HhaI GCGC 5 cut(s) 97, 134, 136, 138, 165
Hin1II CATG 5 cut(s) 312, 364, 408, 475, 618
Hin6I GCGC 5 cut(s) 95, 132, 134, 136, 163
HinP1I GCGC 5 cut(s) 95, 132, 134, 136, 163
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 3 cut(s) 224, 390, 543
HpaII CCGG 1 cut(s) 590
HphI GGTGA 1 cut(s) 71
Hpy188I TCNGA 2 cut(s) 395, 627
Hpy188III TCNNGA 2 cut(s) 462, 637
HpyAV CCTTC 2 cut(s) 92, 132
HpyCH4III ACNGT 5 cut(s) 7, 23, 217, 401, 623
HpyCH4V TGCA 1 cut(s) 62
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 2 cut(s) 159, 185
Hsp92II CATG 5 cut(s) 312, 364, 408, 475, 618
HspAI GCGC 5 cut(s) 95, 132, 134, 136, 163
Kzo9I GATC 1 cut(s) 293
LpnPI CCDG 9 cut(s) 105, 114, 187, 214, 241, 304, 495, 495, 603
Lsp1109I GCAGC 1 cut(s) 86
MaeI CTAG 2 cut(s) 347, 504
MaeIII GTNAC 2 cut(s) 145, 217
MalI GATC 1 cut(s) 295
MauBI CGCGCGCG 1 cut(s) 134
MboI GATC 1 cut(s) 293
MboII GAAGA 2 cut(s) 106, 147
MflI RGATCY 1 cut(s) 293
MhlI GDGCHC 2 cut(s) 53, 413
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 6 cut(s) 333, 448, 454, 465, 495, 630
MluNI TGGCCA 1 cut(s) 339
MlyI GAGTC 2 cut(s) 233, 384
MmeI TCCRAC 3 cut(s) 42, 294, 346
MnlI CCTC 3 cut(s) 128, 525, 596
Mox20I TGGCCA 1 cut(s) 339
MscI TGGCCA 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 2 cut(s) 229, 590
MvaI CCWGG 1 cut(s) 229
MvnI CGCG 5 cut(s) 107, 134, 136, 138, 165
MwoI GCNNNNNNNGC 1 cut(s) 190
NciI CCSGG 1 cut(s) 590
NcoI CCATGG 2 cut(s) 308, 360
NdeII GATC 1 cut(s) 293
NlaIII CATG 5 cut(s) 312, 364, 408, 475, 618
NlaIV GGNNCC 9 cut(s) 16, 51, 52, 206, 268, 356, 410, 411, 445
NmuCI GTSAC 1 cut(s) 217
PauI GCGCGC 2 cut(s) 132, 134
PfeI GAWTC 1 cut(s) 543
PflFI GACNNNGTC 1 cut(s) 223
PflMI CCANNNNNTGG 1 cut(s) 419
PfoI TCCNGGA 1 cut(s) 588
PkrI GCNGC 1 cut(s) 76
PleI GAGTC 2 cut(s) 232, 384
PpsI GAGTC 2 cut(s) 232, 384
PsiI TTATAA 2 cut(s) 285, 332
Psp6I CCWGG 1 cut(s) 227
PspGI CCWGG 1 cut(s) 227
PspN4I GGNNCC 9 cut(s) 16, 51, 52, 206, 268, 356, 410, 411, 445
PspOMI GGGCCC 2 cut(s) 49, 409
PspPI GGNCC 7 cut(s) 49, 50, 204, 266, 409, 410, 512
PstI CTGCAG 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 482
PsuI RGATCY 1 cut(s) 293
PsyI GACNNNGTC 1 cut(s) 223
PteI GCGCGC 2 cut(s) 132, 134
PvuII CAGCTG 1 cut(s) 128
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 1 cut(s) 293
Sau96I GGNCC 7 cut(s) 49, 50, 204, 266, 409, 410, 512
SchI GAGTC 2 cut(s) 233, 384
ScrFI CCNGG 2 cut(s) 229, 590
SduI GDGCHC 2 cut(s) 53, 413
SetI ASST 7 cut(s) 79, 84, 130, 445, 511, 571, 610
SfcI CTRYAG 2 cut(s) 60, 213
SinI GGWCC 1 cut(s) 204
Sse9I AATT 6 cut(s) 333, 448, 454, 465, 495, 630
SsiI CCGC 2 cut(s) 47, 528
SspMI CTAG 2 cut(s) 347, 504
StyD4I CCNGG 2 cut(s) 227, 588
StyI CCWWGG 3 cut(s) 308, 360, 439
TaaI ACNGT 5 cut(s) 7, 23, 217, 401, 623
TaqI TCGA 1 cut(s) 463
TaqII GACCGA 1 cut(s) 236
TasI AATT 6 cut(s) 333, 448, 454, 465, 495, 630
TfiI GAWTC 1 cut(s) 543
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 4 cut(s) 81, 162, 335, 393
TspRI CASTG 1 cut(s) 222
Tth111I GACNNNGTC 1 cut(s) 223
Van91I CCANNNNNTGG 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 204
XapI RAATTY 1 cut(s) 630
XspI CTAG 2 cut(s) 347, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.